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The map label for this gene is purH

Identifier: 83594984

GI number: 83594984

Start: 4194420

End: 4196000

Strand: Reverse

Name: purH

Synonym: Rru_A3655

Alternate gene names: 83594984

Gene position: 4196000-4194420 (Counterclockwise)

Preceding gene: 83594985

Following gene: 83594983

Centisome position: 96.4

GC content: 67.74

Gene sequence:

>1581_bases
GTGCTCCATTCCCTGCCCATCCGCCGCGCCCTGATCAGCGTTTCCGACAAGGGCGGGCTTGTGCCCTTCGCCCGTTTCCT
CGCCGATCACGACATCGAGATCTTGTCGACCGGAGGCAGCGCCAAGGCGCTGGCCGATGCCGGCATTCCGGTGACCGAGG
TCGCCGATTTCACCGGTTTCCCGGAAATGCTCGATGGCCGGGTCAAGACCCTGCATCCGAAGATCCACGGCGGCATCCTG
GGCATCCGCGACAATCCCGAGCACCAGCGGGCGATGGCCGCCCATGAGATCTTGCCGATCGATCTGGTGGTGGTGAACCT
CTATCCCTTCGAAGCCACGGTGGCCAAGGGCGCCGCCTTCGAGGACTGCGTCGAGAACATCGACATCGGTGGGCCGGCCC
TGATCCGCGCCGCCGCCAAGAACCACGAGGCGGTCACCGTCGTCGTCGATCCCGAGGATTACCAGCCGGTGATGGACGCC
ATGACCGCCGAGGGCGGCGCCACCACGCTGGAGCTGCGGCGCAAGCTGGCTTCGGCCGCCTTCGCCCGCTGCGGCGCCTA
TGACGGCGCCATCAGCCGCTGGTTCCAGGGGCAGGTCGGCGACGAGACTCCGCGTCATATCGTTTTCGCCGGCCGCCTGC
GCCAGACCCTGCGCTATGGCGAGAACCCCCATCAGAAGGCGGCGTTCTATGGTCACGGCATCGCCCGCCCGGGGGTGGCC
AGCGCCGAGCAGCTTCAGGGCAAGGAGCTGAGCTACAACAACATCAATGATACCGACGCCGCCTTTGATCTGGTCTGCGA
ATTCGCCGAGCCGGCGGTGGCGATCATCAAGCACGCCAACCCCTGCGGCGTCGCCCAGGGCGCCAGCGTCGTCGAAGCCT
ATAAGGCCGCCCTCGCCTGCGATCCGGTCAGCGCCTTTGGCGGCATCGTCGCCCTCAACCGGCCGATCGATCGCGACTCG
GCGGTGGAAATCACCAAGATCTTCACCGAGGTGGTCATCGCCCCCGATGCCGACGCCGAGGCGCGGGCGATTTTCGCGGC
CAAGAAAAACCTGCGCCTGCTGCTGACCGGCGTGGTCGCCGATACCACGGCGCCCGGGCTGACCGTGCGCTCGGTCGCCG
GCGGCATGCTGGTCCAGGACCGCGACGCCGCCGATCTGCTGTCGGCCGATCTCAAGGTGGTCAGCAAGCGCACGCCGACC
GAACGCGAACTGGCCGACATGCTGATCGCCTTCAAGGTCTGCAAGCACGTCAAATCCAACGCCATCGTCTATGTCAAGGA
TGGCGCCACGGTGGGCATCGGCGCCGGCCAGATGAGCCGGGTCGACAGCGCCCGCATCGCCTCGTGGAAGGCCGATGAGG
CCGCCGAGGCGGCCGGGCTCGCCCAATCGCCGACCCAGGGGTCGGTCGTCGCCTCCGACGCCTTCTTCCCCTTCGCCGAT
GGCCTGCTGGCCGCGGCCAAGGCCGGGGCAACGGCGGTGATCCAGCCCGGCGGCAGCATGCGCGACGACGAGGTCATCAA
AGCCGCCGACGAGGCCGGCTTGGCGATGGTCTTCACCGGTTTGCGCCACTTCCGCCATTAG

Upstream 100 bases:

>100_bases
CCTTTGAGGGCGAGTGCGCCCAGGCGCGCGCCGTGCTATAGCCAGACTCAGCGATCTCCCCGCCGACCAAGCCTTTCCGC
CTCCGCCAAGGATCCCGACC

Downstream 100 bases:

>100_bases
GCCCGTGTGCGGGAACCATAGTCCCCGCACACTGGCCTAACCTAGTGATATCGAAGCAAATCGTCGTCACAGTCTGGTCC
AGACTGCTCGGGATTTGCTT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MLHSLPIRRALISVSDKGGLVPFARFLADHDIEILSTGGSAKALADAGIPVTEVADFTGFPEMLDGRVKTLHPKIHGGIL
GIRDNPEHQRAMAAHEILPIDLVVVNLYPFEATVAKGAAFEDCVENIDIGGPALIRAAAKNHEAVTVVVDPEDYQPVMDA
MTAEGGATTLELRRKLASAAFARCGAYDGAISRWFQGQVGDETPRHIVFAGRLRQTLRYGENPHQKAAFYGHGIARPGVA
SAEQLQGKELSYNNINDTDAAFDLVCEFAEPAVAIIKHANPCGVAQGASVVEAYKAALACDPVSAFGGIVALNRPIDRDS
AVEITKIFTEVVIAPDADAEARAIFAAKKNLRLLLTGVVADTTAPGLTVRSVAGGMLVQDRDAADLLSADLKVVSKRTPT
ERELADMLIAFKVCKHVKSNAIVYVKDGATVGIGAGQMSRVDSARIASWKADEAAEAAGLAQSPTQGSVVASDAFFPFAD
GLLAAAKAGATAVIQPGGSMRDDEVIKAADEAGLAMVFTGLRHFRH

Sequences:

>Translated_526_residues
MLHSLPIRRALISVSDKGGLVPFARFLADHDIEILSTGGSAKALADAGIPVTEVADFTGFPEMLDGRVKTLHPKIHGGIL
GIRDNPEHQRAMAAHEILPIDLVVVNLYPFEATVAKGAAFEDCVENIDIGGPALIRAAAKNHEAVTVVVDPEDYQPVMDA
MTAEGGATTLELRRKLASAAFARCGAYDGAISRWFQGQVGDETPRHIVFAGRLRQTLRYGENPHQKAAFYGHGIARPGVA
SAEQLQGKELSYNNINDTDAAFDLVCEFAEPAVAIIKHANPCGVAQGASVVEAYKAALACDPVSAFGGIVALNRPIDRDS
AVEITKIFTEVVIAPDADAEARAIFAAKKNLRLLLTGVVADTTAPGLTVRSVAGGMLVQDRDAADLLSADLKVVSKRTPT
ERELADMLIAFKVCKHVKSNAIVYVKDGATVGIGAGQMSRVDSARIASWKADEAAEAAGLAQSPTQGSVVASDAFFPFAD
GLLAAAKAGATAVIQPGGSMRDDEVIKAADEAGLAMVFTGLRHFRH
>Mature_526_residues
MLHSLPIRRALISVSDKGGLVPFARFLADHDIEILSTGGSAKALADAGIPVTEVADFTGFPEMLDGRVKTLHPKIHGGIL
GIRDNPEHQRAMAAHEILPIDLVVVNLYPFEATVAKGAAFEDCVENIDIGGPALIRAAAKNHEAVTVVVDPEDYQPVMDA
MTAEGGATTLELRRKLASAAFARCGAYDGAISRWFQGQVGDETPRHIVFAGRLRQTLRYGENPHQKAAFYGHGIARPGVA
SAEQLQGKELSYNNINDTDAAFDLVCEFAEPAVAIIKHANPCGVAQGASVVEAYKAALACDPVSAFGGIVALNRPIDRDS
AVEITKIFTEVVIAPDADAEARAIFAAKKNLRLLLTGVVADTTAPGLTVRSVAGGMLVQDRDAADLLSADLKVVSKRTPT
ERELADMLIAFKVCKHVKSNAIVYVKDGATVGIGAGQMSRVDSARIASWKADEAAEAAGLAQSPTQGSVVASDAFFPFAD
GLLAAAKAGATAVIQPGGSMRDDEVIKAADEAGLAMVFTGLRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=606, Percent_Identity=34.983498349835, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1790439, Length=540, Percent_Identity=55, Blast_Score=544, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71985564, Length=603, Percent_Identity=34.3283582089552, Blast_Score=285, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI71985574, Length=350, Percent_Identity=27.1428571428571, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323768, Length=605, Percent_Identity=33.7190082644628, Blast_Score=286, Evalue=8e-78,
Organism=Saccharomyces cerevisiae, GI6323056, Length=475, Percent_Identity=36, Blast_Score=259, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24649832, Length=600, Percent_Identity=34.3333333333333, Blast_Score=268, Evalue=5e-72,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_RHORT (Q2RN46)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428736.1
- HSSP:   P31335
- ProteinModelPortal:   Q2RN46
- SMR:   Q2RN46
- STRING:   Q2RN46
- GeneID:   3837111
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3655
- NMPDR:   fig|1085.1.peg.1261
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- PhylomeDB:   Q2RN46
- ProtClustDB:   PRK00881
- BioCyc:   RRUB269796:RRU_A3655-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55400; Mature: 55400

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHSLPIRRALISVSDKGGLVPFARFLADHDIEILSTGGSAKALADAGIPVTEVADFTGF
CCCCCCHHHHHEEECCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHCCCCHHHHHHCCCC
PEMLDGRVKTLHPKIHGGILGIRDNPEHQRAMAAHEILPIDLVVVNLYPFEATVAKGAAF
HHHHCCCCEEECCHHCCCEEECCCCCHHHHHHHHHHCCEEEEEEEEEECCHHHHHCCCHH
EDCVENIDIGGPALIRAAAKNHEAVTVVVDPEDYQPVMDAMTAEGGATTLELRRKLASAA
HHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
FARCGAYDGAISRWFQGQVGDETPRHIVFAGRLRQTLRYGENPHQKAAFYGHGIARPGVA
HHHHCCCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCC
SAEQLQGKELSYNNINDTDAAFDLVCEFAEPAVAIIKHANPCGVAQGASVVEAYKAALAC
CHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
DPVSAFGGIVALNRPIDRDSAVEITKIFTEVVIAPDADAEARAIFAAKKNLRLLLTGVVA
CCHHHHCCEEEECCCCCCCHHHHHHHHHHHHEECCCCCCHHHEEEECCCCCEEEEEEHHH
DTTAPGLTVRSVAGGMLVQDRDAADLLSADLKVVSKRTPTERELADMLIAFKVCKHVKSN
CCCCCCEEHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
AIVYVKDGATVGIGAGQMSRVDSARIASWKADEAAEAAGLAQSPTQGSVVASDAFFPFAD
EEEEEECCCEEEECCCCHHHCCHHHHCCCCCHHHHHHCCCCCCCCCCCEEECCCCCHHHH
GLLAAAKAGATAVIQPGGSMRDDEVIKAADEAGLAMVFTGLRHFRH
HHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLHSLPIRRALISVSDKGGLVPFARFLADHDIEILSTGGSAKALADAGIPVTEVADFTGF
CCCCCCHHHHHEEECCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHCCCCHHHHHHCCCC
PEMLDGRVKTLHPKIHGGILGIRDNPEHQRAMAAHEILPIDLVVVNLYPFEATVAKGAAF
HHHHCCCCEEECCHHCCCEEECCCCCHHHHHHHHHHCCEEEEEEEEEECCHHHHHCCCHH
EDCVENIDIGGPALIRAAAKNHEAVTVVVDPEDYQPVMDAMTAEGGATTLELRRKLASAA
HHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
FARCGAYDGAISRWFQGQVGDETPRHIVFAGRLRQTLRYGENPHQKAAFYGHGIARPGVA
HHHHCCCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCC
SAEQLQGKELSYNNINDTDAAFDLVCEFAEPAVAIIKHANPCGVAQGASVVEAYKAALAC
CHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
DPVSAFGGIVALNRPIDRDSAVEITKIFTEVVIAPDADAEARAIFAAKKNLRLLLTGVVA
CCHHHHCCEEEECCCCCCCHHHHHHHHHHHHEECCCCCCHHHEEEECCCCCEEEEEEHHH
DTTAPGLTVRSVAGGMLVQDRDAADLLSADLKVVSKRTPTERELADMLIAFKVCKHVKSN
CCCCCCEEHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
AIVYVKDGATVGIGAGQMSRVDSARIASWKADEAAEAAGLAQSPTQGSVVASDAFFPFAD
EEEEEECCCEEEECCCCHHHCCHHHHCCCCCHHHHHHCCCCCCCCCCCEEECCCCCHHHH
GLLAAAKAGATAVIQPGGSMRDDEVIKAADEAGLAMVFTGLRHFRH
HHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA