Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 83594950

GI number: 83594950

Start: 4160269

End: 4160766

Strand: Reverse

Name: ogt [H]

Synonym: Rru_A3621

Alternate gene names: 83594950

Gene position: 4160766-4160269 (Counterclockwise)

Preceding gene: 83594951

Following gene: 83594949

Centisome position: 95.59

GC content: 72.29

Gene sequence:

>498_bases
ATGCCCCGCATAACCCTTGCCAGCCCTGTGGGGCCCCTGTGGCTAGAGGAGCGGCACGACGCCCTGGTGGCGCTGGGGTG
GGGCGAGGACGAGACCATCCCCCCGCCCGGGGCCGGCGCTCTCGCCGCCACGCCGCTGTTGGCCGAGGCCGCCCTGCAGC
TGGCCGCCTATTTCGCCGGCGCCCGGCAAGGCTTCGATCTGCCGCTGGCCCCGGGCGGCACCGACCATCAGGCGCGGGTC
TGGGCGGCGCTCCGCGAGATCCCCTTCGGACGCACCGAAAGCTATGGCGCCCTCGCCGCCCGGGTCGGTTCTTCGGCGCG
CAGCGTCGGCACCGCCTGCGGGCGCAACCCCTTACCCATCCTTATCCCCTGCCATCGCGTTTTGGCGGCGGCGGGCGCCG
CGGGCGGTTACTCTGGGCGCGGCGGTCTTGAAACCAAGCGCCGCTTGCTCGCCCTTGAAGGGGTGCCTTTGCCCGGAGAA
ACCCTCTCGCTGTTTTAA

Upstream 100 bases:

>100_bases
CAGGGGCCCGGTGTGCGGTCGGCCGCTGACCCAGCCCGACGACCTGCGCCAGGACCTTCTCGGCCGCTGACCACCCCGCT
TCGCCAAAGGAGGCGCCGCC

Downstream 100 bases:

>100_bases
GCCTGTAAAAAAGGAGACTGCTCTTGGCCAAAGCCATTCGCTTTCGCGAGGTCGGCGGACCCGAGGTCCTGACCTTGGAA
ACCGTCGATCTGCCCGATCT

Product: methylated-DNA-(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MPRITLASPVGPLWLEERHDALVALGWGEDETIPPPGAGALAATPLLAEAALQLAAYFAGARQGFDLPLAPGGTDHQARV
WAALREIPFGRTESYGALAARVGSSARSVGTACGRNPLPILIPCHRVLAAAGAAGGYSGRGGLETKRRLLALEGVPLPGE
TLSLF

Sequences:

>Translated_165_residues
MPRITLASPVGPLWLEERHDALVALGWGEDETIPPPGAGALAATPLLAEAALQLAAYFAGARQGFDLPLAPGGTDHQARV
WAALREIPFGRTESYGALAARVGSSARSVGTACGRNPLPILIPCHRVLAAAGAAGGYSGRGGLETKRRLLALEGVPLPGE
TLSLF
>Mature_164_residues
PRITLASPVGPLWLEERHDALVALGWGEDETIPPPGAGALAATPLLAEAALQLAAYFAGARQGFDLPLAPGGTDHQARVW
AALREIPFGRTESYGALAARVGSSARSVGTACGRNPLPILIPCHRVLAAAGAAGGYSGRGGLETKRRLLALEGVPLPGET
LSLF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=169, Percent_Identity=39.0532544378698, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787596, Length=165, Percent_Identity=38.7878787878788, Blast_Score=88, Evalue=3e-19,
Organism=Escherichia coli, GI1788542, Length=90, Percent_Identity=46.6666666666667, Blast_Score=72, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533068, Length=145, Percent_Identity=33.1034482758621, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115533070, Length=145, Percent_Identity=33.1034482758621, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=68, Percent_Identity=44.1176470588235, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16980; Mature: 16848

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRITLASPVGPLWLEERHDALVALGWGEDETIPPPGAGALAATPLLAEAALQLAAYFAG
CCCCCCCCCCCCCHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ARQGFDLPLAPGGTDHQARVWAALREIPFGRTESYGALAARVGSSARSVGTACGRNPLPI
HCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCEE
LIPCHRVLAAAGAAGGYSGRGGLETKRRLLALEGVPLPGETLSLF
EEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEECC
>Mature Secondary Structure 
PRITLASPVGPLWLEERHDALVALGWGEDETIPPPGAGALAATPLLAEAALQLAAYFAG
CCCCCCCCCCCCHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ARQGFDLPLAPGGTDHQARVWAALREIPFGRTESYGALAARVGSSARSVGTACGRNPLPI
HCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCEE
LIPCHRVLAAAGAAGGYSGRGGLETKRRLLALEGVPLPGETLSLF
EEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]