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The map label for this gene is aroE

Identifier: 83594942

GI number: 83594942

Start: 4151909

End: 4152760

Strand: Reverse

Name: aroE

Synonym: Rru_A3613

Alternate gene names: 83594942

Gene position: 4152760-4151909 (Counterclockwise)

Preceding gene: 83594943

Following gene: 83594941

Centisome position: 95.4

GC content: 73.12

Gene sequence:

>852_bases
ATGATCACCGGCAAGGCCCGGGTCGCCGGGGTGATCGGCTGGCCGGTCGGTCATTCGCGCTCGCCCCGTCTGCATGGCTT
CTGGCTGGCCCGCCACGGCATCGATGGCGCCTATGTGCCCTTGGCCGTCGCCCCCGAGGCGCTGGAGCGCGCCCTCGCCG
CTCTGCCCGCCCTGGGGCTGGCCGGGGTCAATGTCACCGTTCCCCATAAGGAGCACGCGCTGGCGCGCATGGACGCCCTG
ACCGAACGGGCGCGGCGGATCGGCGCGGTCAATACCATCGTCTGCCAGAGCGATGGCCGGCTGCTTGGTGACAACACCGA
TGGCTTCGGCTTTCTTGAGAACCTGCGCCAGCGGCGGCCGGACTGGCGGGCCGGGCACGGTCCGGCGGTGGTGCTGGGCG
CCGGCGGCGCGGCGCGGGCGGTCTGCGCCAGCCTGCTCGAGGCGGGCTGTCCGGCCCTGACCCTGGTCAATCGCGATCAG
GGGCGGGCCCGCGCCCTGGCCGAGGCCCTGGCCGCTTGGTCGCCGGTGCCGATCACCCTGGCGACCTGGGACGAGGCGCC
CCGGACCCTGGGCGGCGCGGCGCTGCTGGTCAATACCACCTCGTTGGGCATGGTCGGCCAGCCGCCCCTGGATCTGGACC
TACGGGGCCTCGCGCCGAGCGCCCTGGTCACCGATATCGTCTATGCCCCCCTGGAAACTCCCCTGCTGGCGCGGGCCCGG
GCTTTGGGCCTGGCCACCGTCGATGGCCTGGGCATGCTTCTCCACCAGGGGCGGCCGGGGTTCGAGGCGTGGTTCGGCGT
CGCCCCCGAGGTTGACGACGCGCTGCGCGCCGCGGTTTTGGGTAAACCTTAG

Upstream 100 bases:

>100_bases
TGCAGTTGTTCGCCGCGATCGAGGGCGATCTGTTCTCGATCCAGGGATTACCCCTGTTGCCGCTGCTTGATTTCCTGCGC
CCCCATGGAGTTCTGCTGTC

Downstream 100 bases:

>100_bases
CAAAGGGCTGCCCAGCGATGGCGGCGAAAAGCCTGGGCGATCGCCTGCGGCGGCGGGCGGCACGATGGCCGGTGATCATC
GGGCTGACCGGGTCGATCGG

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MITGKARVAGVIGWPVGHSRSPRLHGFWLARHGIDGAYVPLAVAPEALERALAALPALGLAGVNVTVPHKEHALARMDAL
TERARRIGAVNTIVCQSDGRLLGDNTDGFGFLENLRQRRPDWRAGHGPAVVLGAGGAARAVCASLLEAGCPALTLVNRDQ
GRARALAEALAAWSPVPITLATWDEAPRTLGGAALLVNTTSLGMVGQPPLDLDLRGLAPSALVTDIVYAPLETPLLARAR
ALGLATVDGLGMLLHQGRPGFEAWFGVAPEVDDALRAAVLGKP

Sequences:

>Translated_283_residues
MITGKARVAGVIGWPVGHSRSPRLHGFWLARHGIDGAYVPLAVAPEALERALAALPALGLAGVNVTVPHKEHALARMDAL
TERARRIGAVNTIVCQSDGRLLGDNTDGFGFLENLRQRRPDWRAGHGPAVVLGAGGAARAVCASLLEAGCPALTLVNRDQ
GRARALAEALAAWSPVPITLATWDEAPRTLGGAALLVNTTSLGMVGQPPLDLDLRGLAPSALVTDIVYAPLETPLLARAR
ALGLATVDGLGMLLHQGRPGFEAWFGVAPEVDDALRAAVLGKP
>Mature_283_residues
MITGKARVAGVIGWPVGHSRSPRLHGFWLARHGIDGAYVPLAVAPEALERALAALPALGLAGVNVTVPHKEHALARMDAL
TERARRIGAVNTIVCQSDGRLLGDNTDGFGFLENLRQRRPDWRAGHGPAVVLGAGGAARAVCASLLEAGCPALTLVNRDQ
GRARALAEALAAWSPVPITLATWDEAPRTLGGAALLVNTTSLGMVGQPPLDLDLRGLAPSALVTDIVYAPLETPLLARAR
ALGLATVDGLGMLLHQGRPGFEAWFGVAPEVDDALRAAVLGKP

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=272, Percent_Identity=38.2352941176471, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1787983, Length=273, Percent_Identity=34.7985347985348, Blast_Score=136, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320332, Length=272, Percent_Identity=31.25, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_RHORT (Q2RN88)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428694.1
- HSSP:   Q9SQT8
- ProteinModelPortal:   Q2RN88
- SMR:   Q2RN88
- STRING:   Q2RN88
- GeneID:   3837069
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3613
- NMPDR:   fig|1085.1.peg.1884
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   HGIDGAY
- PhylomeDB:   Q2RN88
- ProtClustDB:   PRK00258
- BioCyc:   RRUB269796:RRU_A3613-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 29455; Mature: 29455

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: ACT_SITE 70-70

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGKARVAGVIGWPVGHSRSPRLHGFWLARHGIDGAYVPLAVAPEALERALAALPALGL
CCCCCCEEEEEEECCCCCCCCCCEEEEEEEECCCCCCEEEHEECHHHHHHHHHHHCCCCC
AGVNVTVPHKEHALARMDALTERARRIGAVNTIVCQSDGRLLGDNTDGFGFLENLRQRRP
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCHHHHHHHHHCCC
DWRAGHGPAVVLGAGGAARAVCASLLEAGCPALTLVNRDQGRARALAEALAAWSPVPITL
CCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEE
ATWDEAPRTLGGAALLVNTTSLGMVGQPPLDLDLRGLAPSALVTDIVYAPLETPLLARAR
EECCCCCHHCCCEEEEEEECCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCCHHHHHHH
ALGLATVDGLGMLLHQGRPGFEAWFGVAPEVDDALRAAVLGKP
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MITGKARVAGVIGWPVGHSRSPRLHGFWLARHGIDGAYVPLAVAPEALERALAALPALGL
CCCCCCEEEEEEECCCCCCCCCCEEEEEEEECCCCCCEEEHEECHHHHHHHHHHHCCCCC
AGVNVTVPHKEHALARMDALTERARRIGAVNTIVCQSDGRLLGDNTDGFGFLENLRQRRP
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCHHHHHHHHHCCC
DWRAGHGPAVVLGAGGAARAVCASLLEAGCPALTLVNRDQGRARALAEALAAWSPVPITL
CCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEE
ATWDEAPRTLGGAALLVNTTSLGMVGQPPLDLDLRGLAPSALVTDIVYAPLETPLLARAR
EECCCCCHHCCCEEEEEEECCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCCHHHHHHH
ALGLATVDGLGMLLHQGRPGFEAWFGVAPEVDDALRAAVLGKP
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA