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The map label for this gene is lutB [H]

Identifier: 83594934

GI number: 83594934

Start: 4144702

End: 4146120

Strand: Reverse

Name: lutB [H]

Synonym: Rru_A3605

Alternate gene names: 83594934

Gene position: 4146120-4144702 (Counterclockwise)

Preceding gene: 83594935

Following gene: 83594933

Centisome position: 95.25

GC content: 67.3

Gene sequence:

>1419_bases
ATGGATCCCAAAAGCCGCGCATTCAAGGACAACGCCCGCAAGGCGCTGACCGACGACACCCTGGCCTCGGCCTTGGGCAC
GATGAAGGCCGGTTTCCGCCGCGCCCGGGCCGAGGCGATCGGGCGATTGCCCGAATTCGACGCCCTGCGCGATGCCGGCC
GCGACCTGAAAAACCATGTTCTGGACAATCTCGATTTCTATCTGGAGACCTTCGAAGCCAAGGTGATCGCCCAGGGCGGC
CACGTTCATTGGTGCCGCGACGCCGCCGAGGCCCGCGAGACCATTTTGGCCATCTGCCGCGACTCGGGCGCGAAAACGGT
GACCAAGGGCAAGTCGATGATCACCGAGGAGATCGGCCTCAACGCCTATCTCGAGGCCAATGGCGTCACGCCGATCGAAA
CCGATCTGGGCGAATACATCATCCAGCTGCGCGGCGAGACCCCCAGCCACATCGTCGCCCCGGCCATCCACCTGCGCCGT
CCCCATGTCGCCGAGGCCTTCCGCAAGGCCCATACCCAGTTCGCTGCCGATCGCCGGCTGGAAGAGCACCGCGATTTCCT
CGACGAGGCCCGCGCGGTGATGCGGGGGAATTTCCTGGCCGCCGATGTCGGCATCACCGGCGCCAATATGATGATCGCCG
AGACCGGATCGACGGTGATCGTCACCAACGAGGGCAATGGCGATCTGACCCAGTTGCTGCCGCGCGTGCATGTCGTGGTC
GCCAGCCTGGAAAAGATCGTGCCGACCCTTGATGACGCCGCCCTGGTGCTGCGCCTGCTCGCCCGTTCGGCCTCGGGTCA
GGACATGAGCGCCTATACCACCTTCTCCACCGGCCCGCGCCGGCCGGACGACGTCGATGGCCCCGACGCCTTTCATGTCG
TGCTGCTCGACAACGGCCGTTCCGACATGCTGGGCACGGGTTTTCAGGAGATGCTGCGCTGCATCCGCTGCGCCGCCTGC
ATGAACCACTGTCCGGTCTATGGCGCCATCGGCGGCCACGCCTATGGCTGGGTCTATCCCGGGCCGATGGGCTCGGTGCT
GACCCCGGCGTTGATCGGGCTGGAGCAGGCGGCCGATCTGCCCAACGCCTCGACGCTGTGCGGGCGCTGCGAAGAGGTTT
GCCCGATGCGCATCCCCTTGCCGCGCATGCTGCGCCACTGGCGCGAAAAGGAATTCGAGCGCCATCTGACGCCAAAGCCG
GCGCGCTTCGGCCTTGGCGCCTGGGCCTTCGTCGCCGCCCGCCCAGGGCTTTACCGGCTGACGACGCGGTTGATCGCCGG
GGCGCTGTCGCGCCTTGGCGGAAAACGCGGCCATCTGCGCTCGCTGGGGCTGGCCAATGGCTGGACGGCGGTGCGCGCCA
TGCCCGCCCCCGAAGGCCGCACCTTCATGGACCGCTGGCGGCAGGGGGAGCGGCCATGA

Upstream 100 bases:

>100_bases
CGAGGTTCTGGCCGGAATGGCCGAGGGCCCCGGATTGGGGGATGGCGCCGAGACCCCGCCAAGCCCTCGCGTCCCCCATC
CGGTCTGAAGGACGCGCCCC

Downstream 100 bases:

>100_bases
CGTCTCCTCCGCCCGAGCGGCCGCCGGAAAACCCCTCCGAGCCGCCGCGGATCGAGCCCGATCTGCCGCAGCCGCCCTCG
GCGGCCGAATTGCGGGCGCG

Product: iron-sulfur cluster binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MDPKSRAFKDNARKALTDDTLASALGTMKAGFRRARAEAIGRLPEFDALRDAGRDLKNHVLDNLDFYLETFEAKVIAQGG
HVHWCRDAAEARETILAICRDSGAKTVTKGKSMITEEIGLNAYLEANGVTPIETDLGEYIIQLRGETPSHIVAPAIHLRR
PHVAEAFRKAHTQFAADRRLEEHRDFLDEARAVMRGNFLAADVGITGANMMIAETGSTVIVTNEGNGDLTQLLPRVHVVV
ASLEKIVPTLDDAALVLRLLARSASGQDMSAYTTFSTGPRRPDDVDGPDAFHVVLLDNGRSDMLGTGFQEMLRCIRCAAC
MNHCPVYGAIGGHAYGWVYPGPMGSVLTPALIGLEQAADLPNASTLCGRCEEVCPMRIPLPRMLRHWREKEFERHLTPKP
ARFGLGAWAFVAARPGLYRLTTRLIAGALSRLGGKRGHLRSLGLANGWTAVRAMPAPEGRTFMDRWRQGERP

Sequences:

>Translated_472_residues
MDPKSRAFKDNARKALTDDTLASALGTMKAGFRRARAEAIGRLPEFDALRDAGRDLKNHVLDNLDFYLETFEAKVIAQGG
HVHWCRDAAEARETILAICRDSGAKTVTKGKSMITEEIGLNAYLEANGVTPIETDLGEYIIQLRGETPSHIVAPAIHLRR
PHVAEAFRKAHTQFAADRRLEEHRDFLDEARAVMRGNFLAADVGITGANMMIAETGSTVIVTNEGNGDLTQLLPRVHVVV
ASLEKIVPTLDDAALVLRLLARSASGQDMSAYTTFSTGPRRPDDVDGPDAFHVVLLDNGRSDMLGTGFQEMLRCIRCAAC
MNHCPVYGAIGGHAYGWVYPGPMGSVLTPALIGLEQAADLPNASTLCGRCEEVCPMRIPLPRMLRHWREKEFERHLTPKP
ARFGLGAWAFVAARPGLYRLTTRLIAGALSRLGGKRGHLRSLGLANGWTAVRAMPAPEGRTFMDRWRQGERP
>Mature_472_residues
MDPKSRAFKDNARKALTDDTLASALGTMKAGFRRARAEAIGRLPEFDALRDAGRDLKNHVLDNLDFYLETFEAKVIAQGG
HVHWCRDAAEARETILAICRDSGAKTVTKGKSMITEEIGLNAYLEANGVTPIETDLGEYIIQLRGETPSHIVAPAIHLRR
PHVAEAFRKAHTQFAADRRLEEHRDFLDEARAVMRGNFLAADVGITGANMMIAETGSTVIVTNEGNGDLTQLLPRVHVVV
ASLEKIVPTLDDAALVLRLLARSASGQDMSAYTTFSTGPRRPDDVDGPDAFHVVLLDNGRSDMLGTGFQEMLRCIRCAAC
MNHCPVYGAIGGHAYGWVYPGPMGSVLTPALIGLEQAADLPNASTLCGRCEEVCPMRIPLPRMLRHWREKEFERHLTPKP
ARFGLGAWAFVAARPGLYRLTTRLIAGALSRLGGKRGHLRSLGLANGWTAVRAMPAPEGRTFMDRWRQGERP

Specific function: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate [H]

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1786498, Length=466, Percent_Identity=38.412017167382, Blast_Score=353, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825 [H]

Pfam domain/function: PF02589 DUF162 [H]

EC number: NA

Molecular weight: Translated: 51747; Mature: 51747

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPKSRAFKDNARKALTDDTLASALGTMKAGFRRARAEAIGRLPEFDALRDAGRDLKNHV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LDNLDFYLETFEAKVIAQGGHVHWCRDAAEARETILAICRDSGAKTVTKGKSMITEEIGL
HHHHHHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
NAYLEANGVTPIETDLGEYIIQLRGETPSHIVAPAIHLRRPHVAEAFRKAHTQFAADRRL
CEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
EEHRDFLDEARAVMRGNFLAADVGITGANMMIAETGSTVIVTNEGNGDLTQLLPRVHVVV
HHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHH
ASLEKIVPTLDDAALVLRLLARSASGQDMSAYTTFSTGPRRPDDVDGPDAFHVVLLDNGR
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCC
SDMLGTGFQEMLRCIRCAACMNHCPVYGAIGGHAYGWVYPGPMGSVLTPALIGLEQAADL
CCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PNASTLCGRCEEVCPMRIPLPRMLRHWREKEFERHLTPKPARFGLGAWAFVAARPGLYRL
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHCCCCHHHH
TTRLIAGALSRLGGKRGHLRSLGLANGWTAVRAMPAPEGRTFMDRWRQGERP
HHHHHHHHHHHCCCCCCCHHHCCCCCCCHHEEECCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MDPKSRAFKDNARKALTDDTLASALGTMKAGFRRARAEAIGRLPEFDALRDAGRDLKNHV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LDNLDFYLETFEAKVIAQGGHVHWCRDAAEARETILAICRDSGAKTVTKGKSMITEEIGL
HHHHHHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
NAYLEANGVTPIETDLGEYIIQLRGETPSHIVAPAIHLRRPHVAEAFRKAHTQFAADRRL
CEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
EEHRDFLDEARAVMRGNFLAADVGITGANMMIAETGSTVIVTNEGNGDLTQLLPRVHVVV
HHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHH
ASLEKIVPTLDDAALVLRLLARSASGQDMSAYTTFSTGPRRPDDVDGPDAFHVVLLDNGR
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCC
SDMLGTGFQEMLRCIRCAACMNHCPVYGAIGGHAYGWVYPGPMGSVLTPALIGLEQAADL
CCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PNASTLCGRCEEVCPMRIPLPRMLRHWREKEFERHLTPKPARFGLGAWAFVAARPGLYRL
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHCCCCHHHH
TTRLIAGALSRLGGKRGHLRSLGLANGWTAVRAMPAPEGRTFMDRWRQGERP
HHHHHHHHHHHCCCCCCCHHHCCCCCCCHHEEECCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA