Click here to switch to the map view.

The map label for this gene is manX [H]

Identifier: 83594776

GI number: 83594776

Start: 3971425

End: 3971829

Strand: Reverse

Name: manX [H]

Synonym: Rru_A3447

Alternate gene names: 83594776

Gene position: 3971829-3971425 (Counterclockwise)

Preceding gene: 83594777

Following gene: 83594775

Centisome position: 91.25

GC content: 61.73

Gene sequence:

>405_bases
ATGATTGGCATGGTTCTGGTGACCCACGGACGTTTGGCGGTGGAATTGATCAACGCCACGGAACACGTCATGGGAAAGCA
GGAGAACATCGCCGCGGTCTGTATCGGCCCGGAGGACGACATGGAGGAGCGGCGCCAGGACATCTTGCGCTGCATCGACA
AGGTCGATACCGGCGACGGGACCATCCTGCTGACCGACATGTTCGGCGGCACCCCGTCCAATCTGGCGATTTCGGTCATG
GAAAAGGCCAAGATCGAAGTGATCGCCGGGGTCAATCTGCCGATGCTGATCCGCTTGGCCACCGTGCGCGACACCCAGTC
GCTGCAAGACGCCGCGACCAGCGCCCAGGAGGCCGGCCGCAAATACATCAACGTCGCCTCGCGGCTGCTGACCCAGAATC
CCTAA

Upstream 100 bases:

>100_bases
AACGAAACGGGGCGGCGGGCCCATGTCCGCCACCGTGACCTCAAGGAGACCCCCCGATGACGCAGGCCGTCCGGGACCTC
GTACCACAGGGATCGACCTC

Downstream 100 bases:

>100_bases
GCGGACGCCTTATCGGCGAGGATGGGGCTGATCCCCCGCGCCTCCGCGACCATATATCCAAAGGACGCGATGGGAATCGC
GACCAAAAGGGCTTGCGGGG

Product: PTS system fructose subfamily IIA component

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MIGMVLVTHGRLAVELINATEHVMGKQENIAAVCIGPEDDMEERRQDILRCIDKVDTGDGTILLTDMFGGTPSNLAISVM
EKAKIEVIAGVNLPMLIRLATVRDTQSLQDAATSAQEAGRKYINVASRLLTQNP

Sequences:

>Translated_134_residues
MIGMVLVTHGRLAVELINATEHVMGKQENIAAVCIGPEDDMEERRQDILRCIDKVDTGDGTILLTDMFGGTPSNLAISVM
EKAKIEVIAGVNLPMLIRLATVRDTQSLQDAATSAQEAGRKYINVASRLLTQNP
>Mature_134_residues
MIGMVLVTHGRLAVELINATEHVMGKQENIAAVCIGPEDDMEERRQDILRCIDKVDTGDGTILLTDMFGGTPSNLAISVM
EKAKIEVIAGVNLPMLIRLATVRDTQSLQDAATSAQEAGRKYINVASRLLTQNP

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=122, Percent_Identity=36.0655737704918, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 14518; Mature: 14518

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGMVLVTHGRLAVELINATEHVMGKQENIAAVCIGPEDDMEERRQDILRCIDKVDTGDG
CCEEEEEECCCEEEEHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
TILLTDMFGGTPSNLAISVMEKAKIEVIAGVNLPMLIRLATVRDTQSLQDAATSAQEAGR
EEEEEECCCCCCCHHHEEEHHHCEEEEEECCCCHHEEEEHHHHCHHHHHHHHHHHHHHHH
KYINVASRLLTQNP
HHHHHHHHHHHCCC
>Mature Secondary Structure
MIGMVLVTHGRLAVELINATEHVMGKQENIAAVCIGPEDDMEERRQDILRCIDKVDTGDG
CCEEEEEECCCEEEEHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
TILLTDMFGGTPSNLAISVMEKAKIEVIAGVNLPMLIRLATVRDTQSLQDAATSAQEAGR
EEEEEECCCCCCCHHHEEEHHHCEEEEEECCCCHHEEEEHHHHCHHHHHHHHHHHHHHHH
KYINVASRLLTQNP
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]