Click here to switch to the map view.

The map label for this gene is trmH [C]

Identifier: 83594701

GI number: 83594701

Start: 3888170

End: 3888781

Strand: Direct

Name: trmH [C]

Synonym: Rru_A3372

Alternate gene names: 83594701

Gene position: 3888170-3888781 (Clockwise)

Preceding gene: 83594699

Following gene: 83594702

Centisome position: 89.33

GC content: 65.52

Gene sequence:

>612_bases
ATGACCACCGCGCTTCGCGGCTATTTTGGACTTGGCGTCGAAGGGGTAAGCAAGCCCTATAACGTCGGCAATGTCTTTCG
CACCGCCCATGCCTTCGGCGCCAGCTTCGTCTTCACCGTCGCGGCCAATTACCGCCAGGAGGAGGGGGCGAAGGTCGACA
CCTCCGACGCCCTGGCCTCCTTGCCGTTCTATCGCTTCGGCGAGGTCGACGACATGATGCTGCCCAAGGGCTGCAAACTG
GTCGGGCTGGAGATCGTGCCCGAGTCGATCGACCTGCCCAGTTTCCGCCATCCGTCCTGCGCCGCCTATGTGCTGGGCGC
CGAACGCCACGGCCTGTCGCCGGCGATGCTGGCGCGCTGCGACCATGTGGTTAAAATTCCCATGCGCTTCTCGATCAATC
TCGGTATGGCCGGGGCGATCGTTCTTTATGACCGGCTGATCACCGTGGGACGCTTCGCCCCGCGCCCGGTTCGGTCGGGC
GGACCGACCGAGGCCCTGGATACCCCGGTGCATGGCGGACCGTTTTTCCGGGCCTCGGGGGCGGCCCATCTGGAGCGCTG
GCGGGCGGCGCCGCCGCTGAACGAGGTGGCGGAGGCCCGTGAAAAAGATTAA

Upstream 100 bases:

>100_bases
GAGAAACGCCCGGACGGGGCTCCCCCTGTCCTTCCATGATGCGGATAACCCGCGCGCGCCGCAACCCGCTGTTCGGCTTC
TTCAATCGGGGACTTTAACG

Downstream 100 bases:

>100_bases
CCACGGGTTGACGCGGGCCCTCTCGCCTGTTTGCATGGCGCCTCAGCCATATCCTGAACATAGTCCTGGCCCAGGCTGCG
GGCTCGGTCACGTCCGCGAC

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: RNA Methyltransferase; 23S RRNA Methyltransferase; RNA Methyltransferase TrmH Family; Cold-Shock RNA Methyltransferase; TrmH Family RNA Methyltransferase; SpoU RRNA Methylase Family Protein; rRNA Methylase-Family Protein

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MTTALRGYFGLGVEGVSKPYNVGNVFRTAHAFGASFVFTVAANYRQEEGAKVDTSDALASLPFYRFGEVDDMMLPKGCKL
VGLEIVPESIDLPSFRHPSCAAYVLGAERHGLSPAMLARCDHVVKIPMRFSINLGMAGAIVLYDRLITVGRFAPRPVRSG
GPTEALDTPVHGGPFFRASGAAHLERWRAAPPLNEVAEAREKD

Sequences:

>Translated_203_residues
MTTALRGYFGLGVEGVSKPYNVGNVFRTAHAFGASFVFTVAANYRQEEGAKVDTSDALASLPFYRFGEVDDMMLPKGCKL
VGLEIVPESIDLPSFRHPSCAAYVLGAERHGLSPAMLARCDHVVKIPMRFSINLGMAGAIVLYDRLITVGRFAPRPVRSG
GPTEALDTPVHGGPFFRASGAAHLERWRAAPPLNEVAEAREKD
>Mature_202_residues
TTALRGYFGLGVEGVSKPYNVGNVFRTAHAFGASFVFTVAANYRQEEGAKVDTSDALASLPFYRFGEVDDMMLPKGCKLV
GLEIVPESIDLPSFRHPSCAAYVLGAERHGLSPAMLARCDHVVKIPMRFSINLGMAGAIVLYDRLITVGRFAPRPVRSGG
PTEALDTPVHGGPFFRASGAAHLERWRAAPPLNEVAEAREKD

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 21949; Mature: 21818

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTALRGYFGLGVEGVSKPYNVGNVFRTAHAFGASFVFTVAANYRQEEGAKVDTSDALAS
CCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCHHEEEEHHHHCHHCCCCCCCCHHHHHH
LPFYRFGEVDDMMLPKGCKLVGLEIVPESIDLPSFRHPSCAAYVLGAERHGLSPAMLARC
CCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHC
DHVVKIPMRFSINLGMAGAIVLYDRLITVGRFAPRPVRSGGPTEALDTPVHGGPFFRASG
CCEEEECEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCEECCC
AAHLERWRAAPPLNEVAEAREKD
HHHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TTALRGYFGLGVEGVSKPYNVGNVFRTAHAFGASFVFTVAANYRQEEGAKVDTSDALAS
CCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCHHEEEEHHHHCHHCCCCCCCCHHHHHH
LPFYRFGEVDDMMLPKGCKLVGLEIVPESIDLPSFRHPSCAAYVLGAERHGLSPAMLARC
CCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHC
DHVVKIPMRFSINLGMAGAIVLYDRLITVGRFAPRPVRSGGPTEALDTPVHGGPFFRASG
CCEEEECEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCEECCC
AAHLERWRAAPPLNEVAEAREKD
HHHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA