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The map label for this gene is fixI [H]

Identifier: 83594653

GI number: 83594653

Start: 3833135

End: 3835564

Strand: Reverse

Name: fixI [H]

Synonym: Rru_A3324

Alternate gene names: 83594653

Gene position: 3835564-3833135 (Counterclockwise)

Preceding gene: 83594654

Following gene: 83594652

Centisome position: 88.12

GC content: 70.53

Gene sequence:

>2430_bases
ATGGACGGACTCTCCGAGACACGGCCGGCGGACTCGGCGGACGGGGGGACGGCGTGCAGGCACTGCGGCTTGCCCGTTCC
GGCCAACGGTCCGGCGGGAGCGGAGTTTTGCTGCGTCGGCTGCCGGGGCGCCTATGGGCTGATCCGCGACAGCGGGCTTG
ACCAGTATTACCAACGGCGCTGTCTTGATCCCGATACGCCGCCGCCCCGTCCCGAGGATCTGGGACCCATCGATTTTTCC
GCCCATATCCTGCGTCAGCCGACGGCGGATGGCGGCGAGGAGGCGGTTCTCCATTTGATGGTCGAGGGCATCCACTGCGC
CGCTTGCGTCTGGCTGATCGAAACCCTGTTGGGGCGCCAGCCCGGCGTATCCCAGGCCCGGCTCAATATGACCACCCGGC
GTCTCACCTTGCGCTTTCGTCCGGCCAAAGGGGAAACCGCCGCCGATCTTGGCGCCAATACCCTGCTCGCCCCGGTGGCG
CGCGTCGGCTATCGGCTGGTGCCCTATGATCCGGCGCTGCTTGATCAGGAAACCCGGCGCACCGAGAAAGCCCTGGTTCA
GGCGATGGCGGTGGCCGGTTTCGCCAGCGCCAATGTCATGCTGCTGTCGGTTTCGGTCTGGGCCGGGGTCGATATGGGGC
CGCGAACCCGCGACCTCATGCACTGGATTTCGGCACTGATCGCCGTTCCCGCCGTGATCTACGCCGTGCGCCCCTTCGCC
CATTCCGCCTTCGCCGCGCTGCGCGCCGGCCGGGCGAGCATGGATATGCCCATTACCCTGGCCGTCACCCTGGCCACCGG
CGTCAGCCTGTGGGAAACCGCCAATGGCGGCGCCCACGCCTATTTCGACGCGGCGGTAACGCTGCTGTTCTTCCTGTTGA
TCGGCCGCTTTCTTGATCACCGGGCGCGTGGCCGCGCCCGCTCGACGGCCGAGCACCTGCTGGCCCTGGGCGCCACCGCC
GTCACCGTGCTGCGCGACGACGGAACATTGGAGCATCGCCCGCCCCGGCAGGTGACCCCGGGGGCGACCGTGCTGGTGGC
CACGGGCGAGCGCATCGGCGTTGATGGCACGGTCAGCCAGGGCTGCTCCGATGTCGATACCAGCCTGCTGACCGGCGAGA
CCCTGCCCGAGCCGGTGCATCCGGGCAGCGCGGTCTTCGCCGGCACCCTCAATCTGACCGGACCGCTGCGCCTGAGCGCC
GGCGCGGTTGGCGAGGGAACGCTGCTGGCCGAGATCGTGCGCATGATGGAGGTCGCCGAACAGGGGCGGGCGCGCTATGT
GGCGATCGCCGACCGGGTGGCGCGCGCCTATGTTCCCGTCGTCCACGCCCTGGCTTTGGCCAGTTTCCTCGGCTGGCTCA
CGCTGATGGGCGCGCCCTGGCAGCAAGCCCTGATGGTGGCGGCGGCCGTGTTGATCGTGACCTGTCCCTGCGCCCTGGCC
CTGGCGGTGCCGGTGGTTCAGGTGGTGGCGACCGGCCGGCTGTTGCGCCAGGGCATTCTGGTGAAATCGCCCACCGCCCT
GGAACGTCTGACCGGCGTCGACCACGTGGTTTTCGACAAGACCGGAACCCTGACCCTGGGGCGCCCCGATCTGCGGCCGG
CCGATCCGGCGCGCGCCGATGCCTGGAGCGCCGAGGATCTCGCCGCCGCCGCCCAACTGGCCGCTGCCAGCCACCATCCC
CTGGCCCGCGCCCTGGCTTTGGCCGCGCCCGAGGCCAAGCCCGCCGCCCTGGCCCGCGAATGGCCGGGGCAGGGCATGAG
CCTGCCTTTGGCCGAGGGCGAGGCGCGACTGGGACGCGCCGTCTTCTGCGGCCTTGAGGACACCCCGGCCGAGGATGGCG
GCGGGCCGGAGCTGTGGTTCACCCGTCCGGGCCATGCGCCGGTGCGCTTTGGCTTTATCGACCGGCCGCGCCCAGACGCG
GCCGAGGTGGTGGCGGCGTTGCGCGCCCAGGGCATCGGCGTGGAATTGCTGTCGGGGGACCGGCCGGCGGTGGCCGCCGC
CCTCGCCGCGCGGATCGGGCTGAACGACTGGCGGGCGGGTTGCTCCCCCGCCGACAAGGTGGCGCGGTTGGCCGAACTGG
CGGCGGCGGGCAAGACCGTGCTGATGGTCGGCGACGGCCTGAACGACGCCCCGGCCCTGGCGGCCGCCCATGTCTCGCTG
TCGCCGGCCTCGGCGGTGGATGTGACGCAGACCGCCGCCGATGTGGTTTTCCAGGGCGAGCGTCTGACCCCGGTGATCGA
GGCGCTGGGCGTCGCCCGCCGCGCCGACCGGCTGGTCCGCCAGAATTTCGTCTTGTCCTTCGCCTATAATATCGTGACGG
TGCCTTTGGCGATCTGTGGTCTGGTAACGCCGCTGATCGCCGCGGTGGCGATGAGCAGTTCCTCGGTGGTCGTTATCCTC
AACGCCCTGCGCCTGTCGCGTCGCCGTTAA

Upstream 100 bases:

>100_bases
CCCTTCGCCGGGCAATGGGAGGTGCGGGTGGTGGCGACGCGCGGTCAGGCGGTGTTCCGCATGCGCGATCGTTTCGTTCT
TACCCCGTAAGGGACCGGTG

Downstream 100 bases:

>100_bases
TCAATTGACTATGGACAAGGGAGGCGTCGATGGAAGCCTTGCTCTATCTGATTCCGATCGCCCTGTTCCTTGGCGCGACC
GGACTTGCCGCTTTTGTCTG

Product: copper-translocating P-type ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 809; Mature: 809

Protein sequence:

>809_residues
MDGLSETRPADSADGGTACRHCGLPVPANGPAGAEFCCVGCRGAYGLIRDSGLDQYYQRRCLDPDTPPPRPEDLGPIDFS
AHILRQPTADGGEEAVLHLMVEGIHCAACVWLIETLLGRQPGVSQARLNMTTRRLTLRFRPAKGETAADLGANTLLAPVA
RVGYRLVPYDPALLDQETRRTEKALVQAMAVAGFASANVMLLSVSVWAGVDMGPRTRDLMHWISALIAVPAVIYAVRPFA
HSAFAALRAGRASMDMPITLAVTLATGVSLWETANGGAHAYFDAAVTLLFFLLIGRFLDHRARGRARSTAEHLLALGATA
VTVLRDDGTLEHRPPRQVTPGATVLVATGERIGVDGTVSQGCSDVDTSLLTGETLPEPVHPGSAVFAGTLNLTGPLRLSA
GAVGEGTLLAEIVRMMEVAEQGRARYVAIADRVARAYVPVVHALALASFLGWLTLMGAPWQQALMVAAAVLIVTCPCALA
LAVPVVQVVATGRLLRQGILVKSPTALERLTGVDHVVFDKTGTLTLGRPDLRPADPARADAWSAEDLAAAAQLAAASHHP
LARALALAAPEAKPAALAREWPGQGMSLPLAEGEARLGRAVFCGLEDTPAEDGGGPELWFTRPGHAPVRFGFIDRPRPDA
AEVVAALRAQGIGVELLSGDRPAVAAALAARIGLNDWRAGCSPADKVARLAELAAAGKTVLMVGDGLNDAPALAAAHVSL
SPASAVDVTQTAADVVFQGERLTPVIEALGVARRADRLVRQNFVLSFAYNIVTVPLAICGLVTPLIAAVAMSSSSVVVIL
NALRLSRRR

Sequences:

>Translated_809_residues
MDGLSETRPADSADGGTACRHCGLPVPANGPAGAEFCCVGCRGAYGLIRDSGLDQYYQRRCLDPDTPPPRPEDLGPIDFS
AHILRQPTADGGEEAVLHLMVEGIHCAACVWLIETLLGRQPGVSQARLNMTTRRLTLRFRPAKGETAADLGANTLLAPVA
RVGYRLVPYDPALLDQETRRTEKALVQAMAVAGFASANVMLLSVSVWAGVDMGPRTRDLMHWISALIAVPAVIYAVRPFA
HSAFAALRAGRASMDMPITLAVTLATGVSLWETANGGAHAYFDAAVTLLFFLLIGRFLDHRARGRARSTAEHLLALGATA
VTVLRDDGTLEHRPPRQVTPGATVLVATGERIGVDGTVSQGCSDVDTSLLTGETLPEPVHPGSAVFAGTLNLTGPLRLSA
GAVGEGTLLAEIVRMMEVAEQGRARYVAIADRVARAYVPVVHALALASFLGWLTLMGAPWQQALMVAAAVLIVTCPCALA
LAVPVVQVVATGRLLRQGILVKSPTALERLTGVDHVVFDKTGTLTLGRPDLRPADPARADAWSAEDLAAAAQLAAASHHP
LARALALAAPEAKPAALAREWPGQGMSLPLAEGEARLGRAVFCGLEDTPAEDGGGPELWFTRPGHAPVRFGFIDRPRPDA
AEVVAALRAQGIGVELLSGDRPAVAAALAARIGLNDWRAGCSPADKVARLAELAAAGKTVLMVGDGLNDAPALAAAHVSL
SPASAVDVTQTAADVVFQGERLTPVIEALGVARRADRLVRQNFVLSFAYNIVTVPLAICGLVTPLIAAVAMSSSSVVVIL
NALRLSRRR
>Mature_809_residues
MDGLSETRPADSADGGTACRHCGLPVPANGPAGAEFCCVGCRGAYGLIRDSGLDQYYQRRCLDPDTPPPRPEDLGPIDFS
AHILRQPTADGGEEAVLHLMVEGIHCAACVWLIETLLGRQPGVSQARLNMTTRRLTLRFRPAKGETAADLGANTLLAPVA
RVGYRLVPYDPALLDQETRRTEKALVQAMAVAGFASANVMLLSVSVWAGVDMGPRTRDLMHWISALIAVPAVIYAVRPFA
HSAFAALRAGRASMDMPITLAVTLATGVSLWETANGGAHAYFDAAVTLLFFLLIGRFLDHRARGRARSTAEHLLALGATA
VTVLRDDGTLEHRPPRQVTPGATVLVATGERIGVDGTVSQGCSDVDTSLLTGETLPEPVHPGSAVFAGTLNLTGPLRLSA
GAVGEGTLLAEIVRMMEVAEQGRARYVAIADRVARAYVPVVHALALASFLGWLTLMGAPWQQALMVAAAVLIVTCPCALA
LAVPVVQVVATGRLLRQGILVKSPTALERLTGVDHVVFDKTGTLTLGRPDLRPADPARADAWSAEDLAAAAQLAAASHHP
LARALALAAPEAKPAALAREWPGQGMSLPLAEGEARLGRAVFCGLEDTPAEDGGGPELWFTRPGHAPVRFGFIDRPRPDA
AEVVAALRAQGIGVELLSGDRPAVAAALAARIGLNDWRAGCSPADKVARLAELAAAGKTVLMVGDGLNDAPALAAAHVSL
SPASAVDVTQTAADVVFQGERLTPVIEALGVARRADRLVRQNFVLSFAYNIVTVPLAICGLVTPLIAAVAMSSSSVVVIL
NALRLSRRR

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=815, Percent_Identity=27.9754601226994, Blast_Score=250, Evalue=3e-66,
Organism=Homo sapiens, GI115529486, Length=861, Percent_Identity=26.3646922183508, Blast_Score=221, Evalue=2e-57,
Organism=Homo sapiens, GI55743073, Length=536, Percent_Identity=30.5970149253731, Blast_Score=183, Evalue=7e-46,
Organism=Homo sapiens, GI48762687, Length=260, Percent_Identity=24.6153846153846, Blast_Score=67, Evalue=9e-11,
Organism=Homo sapiens, GI48762691, Length=267, Percent_Identity=25.0936329588015, Blast_Score=67, Evalue=1e-10,
Organism=Escherichia coli, GI1786691, Length=761, Percent_Identity=31.5374507227332, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1789879, Length=726, Percent_Identity=29.7520661157025, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1786914, Length=515, Percent_Identity=28.1553398058252, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI2367363, Length=151, Percent_Identity=32.4503311258278, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556548, Length=675, Percent_Identity=26.6666666666667, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6319772, Length=617, Percent_Identity=28.6871961102107, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6320475, Length=620, Percent_Identity=26.1290322580645, Blast_Score=191, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6325221, Length=258, Percent_Identity=26.7441860465116, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321430, Length=254, Percent_Identity=25.9842519685039, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221329854, Length=506, Percent_Identity=28.2608695652174, Blast_Score=166, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR000695
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 84738; Mature: 84738

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00154 ATPASE_E1_E2 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGLSETRPADSADGGTACRHCGLPVPANGPAGAEFCCVGCRGAYGLIRDSGLDQYYQRR
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHCCCHHHHHHHH
CLDPDTPPPRPEDLGPIDFSAHILRQPTADGGEEAVLHLMVEGIHCAACVWLIETLLGRQ
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGVSQARLNMTTRRLTLRFRPAKGETAADLGANTLLAPVARVGYRLVPYDPALLDQETRR
CCCCHHHHHEEEEEEEEEEECCCCCCHHHCCCHHHHHHHHHHCCEECCCCHHHCCHHHHH
TEKALVQAMAVAGFASANVMLLSVSVWAGVDMGPRTRDLMHWISALIAVPAVIYAVRPFA
HHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HSAFAALRAGRASMDMPITLAVTLATGVSLWETANGGAHAYFDAAVTLLFFLLIGRFLDH
HHHHHHHHCCCCCCCCCEEEEEEHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RARGRARSTAEHLLALGATAVTVLRDDGTLEHRPPRQVTPGATVLVATGERIGVDGTVSQ
HHCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEECCCCCHHC
GCSDVDTSLLTGETLPEPVHPGSAVFAGTLNLTGPLRLSAGAVGEGTLLAEIVRMMEVAE
CCCHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHH
QGRARYVAIADRVARAYVPVVHALALASFLGWLTLMGAPWQQALMVAAAVLIVTCPCALA
HCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LAVPVVQVVATGRLLRQGILVKSPTALERLTGVDHVVFDKTGTLTLGRPDLRPADPARAD
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCCCCC
AWSAEDLAAAAQLAAASHHPLARALALAAPEAKPAALAREWPGQGMSLPLAEGEARLGRA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHCCCE
VFCGLEDTPAEDGGGPELWFTRPGHAPVRFGFIDRPRPDAAEVVAALRAQGIGVELLSGD
EEECCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCC
RPAVAAALAARIGLNDWRAGCSPADKVARLAELAAAGKTVLMVGDGLNDAPALAAAHVSL
CHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHEEEEEC
SPASAVDVTQTAADVVFQGERLTPVIEALGVARRADRLVRQNFVLSFAYNIVTVPLAICG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTPLIAAVAMSSSSVVVILNALRLSRRR
HHHHHHHHHHCCCCCCEEHHHHHHHHCCC
>Mature Secondary Structure
MDGLSETRPADSADGGTACRHCGLPVPANGPAGAEFCCVGCRGAYGLIRDSGLDQYYQRR
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHCCCHHHHHHHH
CLDPDTPPPRPEDLGPIDFSAHILRQPTADGGEEAVLHLMVEGIHCAACVWLIETLLGRQ
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGVSQARLNMTTRRLTLRFRPAKGETAADLGANTLLAPVARVGYRLVPYDPALLDQETRR
CCCCHHHHHEEEEEEEEEEECCCCCCHHHCCCHHHHHHHHHHCCEECCCCHHHCCHHHHH
TEKALVQAMAVAGFASANVMLLSVSVWAGVDMGPRTRDLMHWISALIAVPAVIYAVRPFA
HHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HSAFAALRAGRASMDMPITLAVTLATGVSLWETANGGAHAYFDAAVTLLFFLLIGRFLDH
HHHHHHHHCCCCCCCCCEEEEEEHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RARGRARSTAEHLLALGATAVTVLRDDGTLEHRPPRQVTPGATVLVATGERIGVDGTVSQ
HHCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEECCCCCHHC
GCSDVDTSLLTGETLPEPVHPGSAVFAGTLNLTGPLRLSAGAVGEGTLLAEIVRMMEVAE
CCCHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHH
QGRARYVAIADRVARAYVPVVHALALASFLGWLTLMGAPWQQALMVAAAVLIVTCPCALA
HCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LAVPVVQVVATGRLLRQGILVKSPTALERLTGVDHVVFDKTGTLTLGRPDLRPADPARAD
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCCCCC
AWSAEDLAAAAQLAAASHHPLARALALAAPEAKPAALAREWPGQGMSLPLAEGEARLGRA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHCCCE
VFCGLEDTPAEDGGGPELWFTRPGHAPVRFGFIDRPRPDAAEVVAALRAQGIGVELLSGD
EEECCCCCCCCCCCCCCEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCC
RPAVAAALAARIGLNDWRAGCSPADKVARLAELAAAGKTVLMVGDGLNDAPALAAAHVSL
CHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHEEEEEC
SPASAVDVTQTAADVVFQGERLTPVIEALGVARRADRLVRQNFVLSFAYNIVTVPLAICG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTPLIAAVAMSSSSVVVILNALRLSRRR
HHHHHHHHHHCCCCCCEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2536685; 11481432 [H]