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The map label for this gene is aphA [H]

Identifier: 83594626

GI number: 83594626

Start: 3791931

End: 3793883

Strand: Direct

Name: aphA [H]

Synonym: Rru_A3296

Alternate gene names: 83594626

Gene position: 3791931-3793883 (Clockwise)

Preceding gene: 83594625

Following gene: 83594627

Centisome position: 87.11

GC content: 64.31

Gene sequence:

>1953_bases
ATGGATACGTCTCCTTCCATCGATCAGGATTTCCGCACCCTGGTTGAAGTCGCGACCGACGCCATCATGGTCATCGGCGG
TGACGGCACCATCCTTTATGCCAATGACGCGGCCGGCGAGTTGTTTGGTCATCGCCCGGCCGACCTGCTGGGCCTGCCCT
TCGGTCATCCGGCGATCCATGGTCAGATCTCCGAACTGGACATCCATGGCCGCGACCGCCGCCGGGTCGCCGAAATGCGC
GTTTCCTCGGTGCATTGGGAAGGCGAGGCCAGCCACGTCATCATCTTGCGCGACGTCAGCGACCGGGTGCGCATGGCGGC
GTCGCTGCGCGACAGATTGGCCTTCGAACAGCTTCTTCTTGAAATCTCGGCGACCTTCGCCCGGGCCCGATCCTCGGAGC
GTCAGACGGCGAGCGCCCAGGCCTTGGGATTGTTTTGCGACTATGTCCGGGCCGAACAGGCCTTTCTGCTGGCGCGGACC
GAGACGGGAGCCTTCGAGATCATCGCCTTTTATCCGGCCGGTGAGCGCGGCAATGATCGGGCCCTATCGCCGGCCCTGGG
CGAGGAGGTGGAAGCCTGCCTGCGCGACAACAGGGTGCAGGCCCTTGTCGGCACCACCCTGGCCGCCAGCGGCGACGGGC
GGACAGACGACTCCGCCCGCTGGATCGGCATCCTGCCCCTGACCGATGGCGAGGAGGGCTTCGGCGCCTTGTGTCTGATG
CGCGCCACCCCGCCCGGCTTTGGCGCGGCGGCCTATGACCTCGAGTCCCTGCGTCCGGCCCCGACGCTTTTTCTCGACGC
CCTGCGCCGTATGCGGCTGGAAGACCTGCTCACCCGCGCCATGGTCAAACAGGCCTCGGTCTTCCGCCAGCTGGTCGAAG
GCCTCTGTCTGGTCCGTGGCCGCCGCGTCGTCCAGGCCAATCAAGCCCTGGCCGACATGATGGGCTATCAGGTCGACGAC
ATGCCCGGGCTGGAGACCAAAGCCTTCTTCGCCACCGAGGAAAGCTATGAGGCGGTCGGGGAGAAAGGGTACCAGGATGT
GCGCGAAGGCCGCCTTTACAGCGCCGAGCACCTGTTCAAGCGACGGACTGGCGAACGCTTCTGGGGCGAGTTGAAAGGCG
CCCCGGTCGAGGGCGAGGATAACGCCGATGGGGAAAGCGTCTGGGTTTTGCGCGATATCACCGATCTGCGCGAGGCGGCC
GCTCGCAAACAGCAACTGATCGACGAATTAAGCCGCTCGAACGCCGAGCTGGAGCGCTTTGCCTTCATCGCCTCCCATGA
TCTGAAGGAGCCGGTGCGCATGGTGGCGTCCTATGTCAACCTGCTGTCGAAGCGCTATGGCAACAGCTTCGATGGCGAAG
GGCGTGAGTTTTTGAACTTCGCCCTGGAGGGGGCGCGGCGCATCCACCAGATGATCGAGGGCATCCTTGATTACGCCCGC
ATCGAGGCGTCCTCCCCCGATCTTGACGCCGTCGCCCTTGAGGGCCCGCTTGATCGCGCCCTGGCAAGCCTCGCCGATAC
CTTGCGCGACTCCGAGGCGCGGATCACCGTCCAGCGCCCTTTGCCCACCGTTCTCGCCGTTCCGTCCGAAATGGAACGGG
TCTTCGTCAATCTGATCGGCAATGCCGTCAAATTCGCCAAACCCGATCAATCCCCGCACATCGACATCATGGTCGAGACC
TGCCCAAGCCCGCCGAAAGGGAAGGATCAGGGATCTCCGCTTTGCCATATCACCATCCGCGATGCCGGGATCGGCATTCC
CGAGGCGGCGCGCGAAAAGGTCTTTGATATTTTCTGGCGGCTGCATGGCCGGGAGAAATACGACGGCACCGGCCTGGGCC
TGGCCATCGTCAAGCGCATCATCGATCGCCGGGGCGGCCGGATCTGGATCGAATCCCGCGAAGGAACGGGAACCACCATC
CATATCCTCCAGCCCCTGGCGCCGAGCCCATAA

Upstream 100 bases:

>100_bases
CCTTCCGCACCGTGACCGGCATCCTGGCCGGCAATCCCCAATACTCCCACCAAAGCGAGGCCGACCGCCTCGACAGACTA
TTTCCCAAGTGACGGCATCG

Downstream 100 bases:

>100_bases
ACCACCGCCCTGGGCGATAAATCCGCAGGGCGCAACCGTTTGCCTGAAAAATCGGCAAAACCCGATCTCCTTCCAGGCCG
GGAGTGGACAAGCAAATCCC

Product: PAS/PAC sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MDTSPSIDQDFRTLVEVATDAIMVIGGDGTILYANDAAGELFGHRPADLLGLPFGHPAIHGQISELDIHGRDRRRVAEMR
VSSVHWEGEASHVIILRDVSDRVRMAASLRDRLAFEQLLLEISATFARARSSERQTASAQALGLFCDYVRAEQAFLLART
ETGAFEIIAFYPAGERGNDRALSPALGEEVEACLRDNRVQALVGTTLAASGDGRTDDSARWIGILPLTDGEEGFGALCLM
RATPPGFGAAAYDLESLRPAPTLFLDALRRMRLEDLLTRAMVKQASVFRQLVEGLCLVRGRRVVQANQALADMMGYQVDD
MPGLETKAFFATEESYEAVGEKGYQDVREGRLYSAEHLFKRRTGERFWGELKGAPVEGEDNADGESVWVLRDITDLREAA
ARKQQLIDELSRSNAELERFAFIASHDLKEPVRMVASYVNLLSKRYGNSFDGEGREFLNFALEGARRIHQMIEGILDYAR
IEASSPDLDAVALEGPLDRALASLADTLRDSEARITVQRPLPTVLAVPSEMERVFVNLIGNAVKFAKPDQSPHIDIMVET
CPSPPKGKDQGSPLCHITIRDAGIGIPEAAREKVFDIFWRLHGREKYDGTGLGLAIVKRIIDRRGGRIWIESREGTGTTI
HILQPLAPSP

Sequences:

>Translated_650_residues
MDTSPSIDQDFRTLVEVATDAIMVIGGDGTILYANDAAGELFGHRPADLLGLPFGHPAIHGQISELDIHGRDRRRVAEMR
VSSVHWEGEASHVIILRDVSDRVRMAASLRDRLAFEQLLLEISATFARARSSERQTASAQALGLFCDYVRAEQAFLLART
ETGAFEIIAFYPAGERGNDRALSPALGEEVEACLRDNRVQALVGTTLAASGDGRTDDSARWIGILPLTDGEEGFGALCLM
RATPPGFGAAAYDLESLRPAPTLFLDALRRMRLEDLLTRAMVKQASVFRQLVEGLCLVRGRRVVQANQALADMMGYQVDD
MPGLETKAFFATEESYEAVGEKGYQDVREGRLYSAEHLFKRRTGERFWGELKGAPVEGEDNADGESVWVLRDITDLREAA
ARKQQLIDELSRSNAELERFAFIASHDLKEPVRMVASYVNLLSKRYGNSFDGEGREFLNFALEGARRIHQMIEGILDYAR
IEASSPDLDAVALEGPLDRALASLADTLRDSEARITVQRPLPTVLAVPSEMERVFVNLIGNAVKFAKPDQSPHIDIMVET
CPSPPKGKDQGSPLCHITIRDAGIGIPEAAREKVFDIFWRLHGREKYDGTGLGLAIVKRIIDRRGGRIWIESREGTGTTI
HILQPLAPSP
>Mature_650_residues
MDTSPSIDQDFRTLVEVATDAIMVIGGDGTILYANDAAGELFGHRPADLLGLPFGHPAIHGQISELDIHGRDRRRVAEMR
VSSVHWEGEASHVIILRDVSDRVRMAASLRDRLAFEQLLLEISATFARARSSERQTASAQALGLFCDYVRAEQAFLLART
ETGAFEIIAFYPAGERGNDRALSPALGEEVEACLRDNRVQALVGTTLAASGDGRTDDSARWIGILPLTDGEEGFGALCLM
RATPPGFGAAAYDLESLRPAPTLFLDALRRMRLEDLLTRAMVKQASVFRQLVEGLCLVRGRRVVQANQALADMMGYQVDD
MPGLETKAFFATEESYEAVGEKGYQDVREGRLYSAEHLFKRRTGERFWGELKGAPVEGEDNADGESVWVLRDITDLREAA
ARKQQLIDELSRSNAELERFAFIASHDLKEPVRMVASYVNLLSKRYGNSFDGEGREFLNFALEGARRIHQMIEGILDYAR
IEASSPDLDAVALEGPLDRALASLADTLRDSEARITVQRPLPTVLAVPSEMERVFVNLIGNAVKFAKPDQSPHIDIMVET
CPSPPKGKDQGSPLCHITIRDAGIGIPEAAREKVFDIFWRLHGREKYDGTGLGLAIVKRIIDRRGGRIWIESREGTGTTI
HILQPLAPSP

Specific function: Photoreceptor which exists in two forms that are reversibly interconvertible by light:the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region [H]

COG id: COG4251

COG function: function code T; Bacteriophytochrome (light-regulated signal transduction histidine kinase)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=278, Percent_Identity=26.2589928057554, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI48994928, Length=271, Percent_Identity=25.830258302583, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1786912, Length=252, Percent_Identity=28.1746031746032, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1790436, Length=248, Percent_Identity=25, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788713, Length=285, Percent_Identity=23.5087719298246, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788549, Length=278, Percent_Identity=26.2589928057554, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87082128, Length=271, Percent_Identity=25.0922509225092, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR013654
- InterPro:   IPR016132
- InterPro:   IPR001294
- InterPro:   IPR013515
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF01590 GAF; PF02518 HATPase_c; PF00512 HisKA; PF08446 PAS_2; PF00360 Phytochrome [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 71705; Mature: 71705

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSPSIDQDFRTLVEVATDAIMVIGGDGTILYANDAAGELFGHRPADLLGLPFGHPAIH
CCCCCCCCHHHHHHHHHHCCEEEEECCCCEEEEECCCCHHHCCCCCHHHHCCCCCCCHHC
GQISELDIHGRDRRRVAEMRVSSVHWEGEASHVIILRDVSDRVRMAASLRDRLAFEQLLL
CEEEEEECCCCHHHHHHHHHHHHEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
EISATFARARSSERQTASAQALGLFCDYVRAEQAFLLARTETGAFEIIAFYPAGERGNDR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEEEEECCCCCCCC
ALSPALGEEVEACLRDNRVQALVGTTLAASGDGRTDDSARWIGILPLTDGEEGFGALCLM
CCCCHHHHHHHHHHHCCCHHHHHHHHEECCCCCCCCCCCCEEEEEEECCCCCCCCEEEEE
RATPPGFGAAAYDLESLRPAPTLFLDALRRMRLEDLLTRAMVKQASVFRQLVEGLCLVRG
ECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RRVVQANQALADMMGYQVDDMPGLETKAFFATEESYEAVGEKGYQDVREGRLYSAEHLFK
CHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHCCCHHHHHHHH
RRTGERFWGELKGAPVEGEDNADGESVWVLRDITDLREAAARKQQLIDELSRSNAELERF
HHCCHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AFIASHDLKEPVRMVASYVNLLSKRYGNSFDGEGREFLNFALEGARRIHQMIEGILDYAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEASSPDLDAVALEGPLDRALASLADTLRDSEARITVQRPLPTVLAVPSEMERVFVNLIG
HCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCHHHHHHHHHHHH
NAVKFAKPDQSPHIDIMVETCPSPPKGKDQGSPLCHITIRDAGIGIPEAAREKVFDIFWR
HHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
LHGREKYDGTGLGLAIVKRIIDRRGGRIWIESREGTGTTIHILQPLAPSP
HCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MDTSPSIDQDFRTLVEVATDAIMVIGGDGTILYANDAAGELFGHRPADLLGLPFGHPAIH
CCCCCCCCHHHHHHHHHHCCEEEEECCCCEEEEECCCCHHHCCCCCHHHHCCCCCCCHHC
GQISELDIHGRDRRRVAEMRVSSVHWEGEASHVIILRDVSDRVRMAASLRDRLAFEQLLL
CEEEEEECCCCHHHHHHHHHHHHEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
EISATFARARSSERQTASAQALGLFCDYVRAEQAFLLARTETGAFEIIAFYPAGERGNDR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEEEEECCCCCCCC
ALSPALGEEVEACLRDNRVQALVGTTLAASGDGRTDDSARWIGILPLTDGEEGFGALCLM
CCCCHHHHHHHHHHHCCCHHHHHHHHEECCCCCCCCCCCCEEEEEEECCCCCCCCEEEEE
RATPPGFGAAAYDLESLRPAPTLFLDALRRMRLEDLLTRAMVKQASVFRQLVEGLCLVRG
ECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RRVVQANQALADMMGYQVDDMPGLETKAFFATEESYEAVGEKGYQDVREGRLYSAEHLFK
CHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHCCCHHHHHHHH
RRTGERFWGELKGAPVEGEDNADGESVWVLRDITDLREAAARKQQLIDELSRSNAELERF
HHCCHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AFIASHDLKEPVRMVASYVNLLSKRYGNSFDGEGREFLNFALEGARRIHQMIEGILDYAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEASSPDLDAVALEGPLDRALASLADTLRDSEARITVQRPLPTVLAVPSEMERVFVNLIG
HCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCHHHHHHHHHHHH
NAVKFAKPDQSPHIDIMVETCPSPPKGKDQGSPLCHITIRDAGIGIPEAAREKVFDIFWR
HHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
LHGREKYDGTGLGLAIVKRIIDRRGGRIWIESREGTGTTIHILQPLAPSP
HCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]