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The map label for this gene is gloB

Identifier: 83594600

GI number: 83594600

Start: 3768588

End: 3769358

Strand: Direct

Name: gloB

Synonym: Rru_A3270

Alternate gene names: 83594600

Gene position: 3768588-3769358 (Clockwise)

Preceding gene: 83594598

Following gene: 83594601

Centisome position: 86.58

GC content: 66.93

Gene sequence:

>771_bases
TTGAGCACTCTCGACATCCATCAGATCGCGGTCCTTTCGGACAATTACATCTATCTGGTCCGCTGCCGGGCCACCGGCGC
CTGCGCGGTCATCGACCCGTCGCTGGCCGAGCCGGTCCTGGCGGCGGCCGAAAGCCTGGGCTGGACGATCACCCATATCC
TCAACACCCACCACCACTATGACCACACCGGCGGCAACGAGGAAATCAAGGCCGCCACCGGCTGCGAGATCATCGGCTTC
GCCGGCGACGCCCATCGCCTGCCCGGGATCGACCGCACCGTGGTGGAAGGCGACCGCGTGGCGATCGGTCAGGCCGAAGC
GCGGGTCATCGAGACTCCCGGCCACACCCTGGGCCATATCGCCTATTGGTTTGCCGAATCGTCGGCGCTGTTTTGCGGCG
ACACGCTGTTTTCGGCCGGCTGTGGCCGGTTGTTCGAGGGGTCGGCCGGTCAGATGTGGGACTCGCTGCGCAAGCTGCGC
GCCCTGCCGGCCCAGACCCTGGTTTTCTGCGGCCATGAATATACCCAGCCCAACATCACCTTCGCCCTGACCATCGACCC
GCGCAACGAGGCCCTGCGCGCCCGGGCGCTGGAGGTCGACGCCCTGCGCGCCGCCGGCCGGCCGACCGTGCCGGCCTTCC
TCGGCGACGAGGCGCGCTCCAATCCGTTCCTGCGCGCCGATTCGGCCGATTTCCAAGAGGCCTTTGGCATGACCGGCGCC
GATCCGGTCGAGGTTTTCGCCCGTACCCGTCTCAAGAAGGATCATTTCTGA

Upstream 100 bases:

>100_bases
AGCGCGTGAGCCGGGTTGGGCGCATCGCGCGAAACGGGCGGCGGAACGATCCCCCCGCTTCAGATCACCGCCGCGAGGCC
GGCCAAAGCAGGACAGGACC

Downstream 100 bases:

>100_bases
CCATGACCGACGCCCCCCGCTTCCGCCTGCGCCATTCGCCGACCTCGCCCTTCGTGCGCAAGGTTCTCGCCTTCGCCATC
GAAACCGGCCTGCGCGATCG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSTLDIHQIAVLSDNYIYLVRCRATGACAVIDPSLAEPVLAAAESLGWTITHILNTHHHYDHTGGNEEIKAATGCEIIGF
AGDAHRLPGIDRTVVEGDRVAIGQAEARVIETPGHTLGHIAYWFAESSALFCGDTLFSAGCGRLFEGSAGQMWDSLRKLR
ALPAQTLVFCGHEYTQPNITFALTIDPRNEALRARALEVDALRAAGRPTVPAFLGDEARSNPFLRADSADFQEAFGMTGA
DPVEVFARTRLKKDHF

Sequences:

>Translated_256_residues
MSTLDIHQIAVLSDNYIYLVRCRATGACAVIDPSLAEPVLAAAESLGWTITHILNTHHHYDHTGGNEEIKAATGCEIIGF
AGDAHRLPGIDRTVVEGDRVAIGQAEARVIETPGHTLGHIAYWFAESSALFCGDTLFSAGCGRLFEGSAGQMWDSLRKLR
ALPAQTLVFCGHEYTQPNITFALTIDPRNEALRARALEVDALRAAGRPTVPAFLGDEARSNPFLRADSADFQEAFGMTGA
DPVEVFARTRLKKDHF
>Mature_255_residues
STLDIHQIAVLSDNYIYLVRCRATGACAVIDPSLAEPVLAAAESLGWTITHILNTHHHYDHTGGNEEIKAATGCEIIGFA
GDAHRLPGIDRTVVEGDRVAIGQAEARVIETPGHTLGHIAYWFAESSALFCGDTLFSAGCGRLFEGSAGQMWDSLRKLRA
LPAQTLVFCGHEYTQPNITFALTIDPRNEALRARALEVDALRAAGRPTVPAFLGDEARSNPFLRADSADFQEAFGMTGAD
PVEVFARTRLKKDHF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538322, Length=261, Percent_Identity=37.9310344827586, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=37.6923076923077, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI14150041, Length=256, Percent_Identity=36.328125, Blast_Score=157, Evalue=7e-39,
Organism=Homo sapiens, GI116642887, Length=234, Percent_Identity=38.4615384615385, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI21703352, Length=234, Percent_Identity=38.4615384615385, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=38.3838383838384, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI41327741, Length=188, Percent_Identity=28.7234042553192, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786406, Length=255, Percent_Identity=41.9607843137255, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787158, Length=176, Percent_Identity=30.1136363636364, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=254, Percent_Identity=36.6141732283465, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17538952, Length=203, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320478, Length=266, Percent_Identity=31.5789473684211, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6324614, Length=260, Percent_Identity=31.9230769230769, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356335, Length=259, Percent_Identity=38.996138996139, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24667711, Length=262, Percent_Identity=38.5496183206107, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24667703, Length=259, Percent_Identity=38.996138996139, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI221330176, Length=167, Percent_Identity=31.7365269461078, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_RHORT (Q2RP80)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428352.1
- ProteinModelPortal:   Q2RP80
- SMR:   Q2RP80
- STRING:   Q2RP80
- GeneID:   3836717
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3270
- NMPDR:   fig|1085.1.peg.911
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- PhylomeDB:   Q2RP80
- ProtClustDB:   CLSK931492
- BioCyc:   RRUB269796:RRU_A3270-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 27761; Mature: 27629

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLDIHQIAVLSDNYIYLVRCRATGACAVIDPSLAEPVLAAAESLGWTITHILNTHHHY
CCCCCCEEEEEEECCEEEEEEECCCCCEEEECCHHHHHHHHHHHHCCEEEEHHHHCCCCC
DHTGGNEEIKAATGCEIIGFAGDAHRLPGIDRTVVEGDRVAIGQAEARVIETPGHTLGHI
CCCCCCCEEECCCCCEEEEECCCCCCCCCCCCEEECCCEEEECCCCEEEEECCCCHHHHE
AYWFAESSALFCGDTLFSAGCGRLFEGSAGQMWDSLRKLRALPAQTLVFCGHEYTQPNIT
EEEEECCCEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEE
FALTIDPRNEALRARALEVDALRAAGRPTVPAFLGDEARSNPFLRADSADFQEAFGMTGA
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCEEECCCCCHHHHHCCCCC
DPVEVFARTRLKKDHF
CHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STLDIHQIAVLSDNYIYLVRCRATGACAVIDPSLAEPVLAAAESLGWTITHILNTHHHY
CCCCCEEEEEEECCEEEEEEECCCCCEEEECCHHHHHHHHHHHHCCEEEEHHHHCCCCC
DHTGGNEEIKAATGCEIIGFAGDAHRLPGIDRTVVEGDRVAIGQAEARVIETPGHTLGHI
CCCCCCCEEECCCCCEEEEECCCCCCCCCCCCEEECCCEEEECCCCEEEEECCCCHHHHE
AYWFAESSALFCGDTLFSAGCGRLFEGSAGQMWDSLRKLRALPAQTLVFCGHEYTQPNIT
EEEEECCCEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEE
FALTIDPRNEALRARALEVDALRAAGRPTVPAFLGDEARSNPFLRADSADFQEAFGMTGA
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCEEECCCCCHHHHHCCCCC
DPVEVFARTRLKKDHF
CHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA