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The map label for this gene is 83594562

Identifier: 83594562

GI number: 83594562

Start: 3726337

End: 3727875

Strand: Direct

Name: 83594562

Synonym: Rru_A3232

Alternate gene names: NA

Gene position: 3726337-3727875 (Clockwise)

Preceding gene: 83594553

Following gene: 83594563

Centisome position: 85.61

GC content: 68.62

Gene sequence:

>1539_bases
ATGCCAACCCGCAGTTCCCCAGCCGCCGTCTCTCCGTCCACCGCCTATGCCGCCCTGGAACGCCGCTTCGCCCGGCTGAG
GGCCATCGAAGACTCGATCGCCTTCCTCCATTGGGATCAGTCGGCGGTGATGCCCGCTGGCGGGGCGTCAAGCCGCGCCG
ATCAGCTCGCCACCCTGGCCGGTCTCCACCACGACCTGCTGACCGCGCCCGACACCGCCGAGATGATCGCCCTGGCCGAT
CCGCTGGCCGAAGCCGAAAGCGCCGAGGACGGGGCGTGGCGGCTGGCCAATCTGGCCGAGATCACCCGCATCCATGCCCA
TGCCATGGCCCTGCCCGGCGATCTGGTCGAAGCCCTGTCGCGGGCCGGCAACGCCTGCGAGCGGGTCTGGCGCGAGGCCC
GGCCACTGGGCGATTTCGCCCTGGTCGAAGGCCCGCTCGGCCAATTGCTTGGTCTGGTGCGCGACTCGGCGGCCGCCAAG
GCCGAGGCCCTGGGCGTCGCCCCCTATGACGCCCTGCTCGATCAATACGAGCCCGGCGGCTCCTCGGCCCGCATCGATGG
GATCTTCGATGATCTGGAGGCCTTCCTGCCCGATTTCCTCGGCCGGGTGATCGAGCGCCAGGGAGCGGCCGCCGAGGCGC
CCGCCGGTCCCTTCCCGATTCCCACCCAACAGGCGGTCGGCCGGGCGACGATGGAAAGCCTGGGCTTTGATTTCGACCAC
GGCCGCCTGGATGTCTCGCTCCATCCGTTTTGCGGCGGCTCGCCCCAGGATCTGCGGCTGACCACGCGCTATGACGAAGC
CGATTTCACCAGCGCCCTGATGGGGGTGATCCACGAAACCGGCCATGCGCTGTATGAACAGGGCCTGCCGCGCGACCGCC
TGTCCCAGCCCGTCGGCCGGGCGCGGGGGATGAGTGTTCATGAAAGCCAGTCGCTGCTTTTCGAAATGCAGGCCGGGCGC
TCGCGCGCCTTCATCAGCCATTGCGCCCCGGCCTGGAAGGCGGCCTTCGGCGGCGAGGGTGAGGCGTGGCGGGCCGACAG
CCTGTATCGCCATTTCACCCGGGTCGAGCCCGGCTTCATCCGCGTCGATGCCGACGAGGTGACCTATCCCGCCCATGTCA
TCGTCCGCTATCGCCTGGAAAAGGCCCTGATCGAAGGACGGATGGCGCTGGGCGATCTGCCGACGGCCTGGAACGACGCC
ATGGAACGGCTGCTGGGCGTGCGCCCGCCTAACGACCGCCTGGGCTGCCTGCAAGATATCCATTGGTATGATGGCGCCTG
GGGGTATTTCCCCACCTATACGCTGGGAGCGATGACCGCCGCCCAGGTGTTTGACGCGGCGAGCCGGGCGGTTCCCGATA
TCGGGCCGGCGATCGGCCGGGGTGATTTCACCCCGCTGCTCGGCTGGCTGCGCACGCATATCCACGCCAAGGGATGCCTG
CTGTCGACCGACGCCCTGCTGCGCGAAGCCACCGGCCGACCGCTCGACGCCAGTGTGTTCAAAGCGCATCTGACGCGGCG
TTATCTGGAAGACCGCTAA

Upstream 100 bases:

>100_bases
GCGAACCTGGGCTTGTCTGTCATAGTCGTTCCTCTCCATATTCCGGGTGGGACGGGTCTCTCGCTCCGTTCTCACTCCAG
GTTAGGACCCGGACATCGCC

Downstream 100 bases:

>100_bases
GCTTGCCCGCCGGGGAACGGGGCATTACTGTCCCGCCGGCGAGCGCGCTTTCCCGCCACCCGTGCTGGAGTCCGAACAGG
TGAGATATGTAAGCACACGG

Product: thermostable carboxypeptidase 1

Products: NA

Alternate protein names: Carboxypeptidase Taq [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MPTRSSPAAVSPSTAYAALERRFARLRAIEDSIAFLHWDQSAVMPAGGASSRADQLATLAGLHHDLLTAPDTAEMIALAD
PLAEAESAEDGAWRLANLAEITRIHAHAMALPGDLVEALSRAGNACERVWREARPLGDFALVEGPLGQLLGLVRDSAAAK
AEALGVAPYDALLDQYEPGGSSARIDGIFDDLEAFLPDFLGRVIERQGAAAEAPAGPFPIPTQQAVGRATMESLGFDFDH
GRLDVSLHPFCGGSPQDLRLTTRYDEADFTSALMGVIHETGHALYEQGLPRDRLSQPVGRARGMSVHESQSLLFEMQAGR
SRAFISHCAPAWKAAFGGEGEAWRADSLYRHFTRVEPGFIRVDADEVTYPAHVIVRYRLEKALIEGRMALGDLPTAWNDA
MERLLGVRPPNDRLGCLQDIHWYDGAWGYFPTYTLGAMTAAQVFDAASRAVPDIGPAIGRGDFTPLLGWLRTHIHAKGCL
LSTDALLREATGRPLDASVFKAHLTRRYLEDR

Sequences:

>Translated_512_residues
MPTRSSPAAVSPSTAYAALERRFARLRAIEDSIAFLHWDQSAVMPAGGASSRADQLATLAGLHHDLLTAPDTAEMIALAD
PLAEAESAEDGAWRLANLAEITRIHAHAMALPGDLVEALSRAGNACERVWREARPLGDFALVEGPLGQLLGLVRDSAAAK
AEALGVAPYDALLDQYEPGGSSARIDGIFDDLEAFLPDFLGRVIERQGAAAEAPAGPFPIPTQQAVGRATMESLGFDFDH
GRLDVSLHPFCGGSPQDLRLTTRYDEADFTSALMGVIHETGHALYEQGLPRDRLSQPVGRARGMSVHESQSLLFEMQAGR
SRAFISHCAPAWKAAFGGEGEAWRADSLYRHFTRVEPGFIRVDADEVTYPAHVIVRYRLEKALIEGRMALGDLPTAWNDA
MERLLGVRPPNDRLGCLQDIHWYDGAWGYFPTYTLGAMTAAQVFDAASRAVPDIGPAIGRGDFTPLLGWLRTHIHAKGCL
LSTDALLREATGRPLDASVFKAHLTRRYLEDR
>Mature_511_residues
PTRSSPAAVSPSTAYAALERRFARLRAIEDSIAFLHWDQSAVMPAGGASSRADQLATLAGLHHDLLTAPDTAEMIALADP
LAEAESAEDGAWRLANLAEITRIHAHAMALPGDLVEALSRAGNACERVWREARPLGDFALVEGPLGQLLGLVRDSAAAKA
EALGVAPYDALLDQYEPGGSSARIDGIFDDLEAFLPDFLGRVIERQGAAAEAPAGPFPIPTQQAVGRATMESLGFDFDHG
RLDVSLHPFCGGSPQDLRLTTRYDEADFTSALMGVIHETGHALYEQGLPRDRLSQPVGRARGMSVHESQSLLFEMQAGRS
RAFISHCAPAWKAAFGGEGEAWRADSLYRHFTRVEPGFIRVDADEVTYPAHVIVRYRLEKALIEGRMALGDLPTAWNDAM
ERLLGVRPPNDRLGCLQDIHWYDGAWGYFPTYTLGAMTAAQVFDAASRAVPDIGPAIGRGDFTPLLGWLRTHIHAKGCLL
STDALLREATGRPLDASVFKAHLTRRYLEDR

Specific function: Releases amino acids sequentially from the C-terminus with a broad substrate specificity (except for proline) [H]

COG id: COG2317

COG function: function code E; Zn-dependent carboxypeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001333 [H]

Pfam domain/function: PF02074 Peptidase_M32 [H]

EC number: =3.4.17.19 [H]

Molecular weight: Translated: 55561; Mature: 55430

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTRSSPAAVSPSTAYAALERRFARLRAIEDSIAFLHWDQSAVMPAGGASSRADQLATLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHH
GLHHDLLTAPDTAEMIALADPLAEAESAEDGAWRLANLAEITRIHAHAMALPGDLVEALS
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RAGNACERVWREARPLGDFALVEGPLGQLLGLVRDSAAAKAEALGVAPYDALLDQYEPGG
HHCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
SSARIDGIFDDLEAFLPDFLGRVIERQGAAAEAPAGPFPIPTQQAVGRATMESLGFDFDH
CCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
GRLDVSLHPFCGGSPQDLRLTTRYDEADFTSALMGVIHETGHALYEQGLPRDRLSQPVGR
CEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHH
ARGMSVHESQSLLFEMQAGRSRAFISHCAPAWKAAFGGEGEAWRADSLYRHFTRVEPGFI
HCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEE
RVDADEVTYPAHVIVRYRLEKALIEGRMALGDLPTAWNDAMERLLGVRPPNDRLGCLQDI
EECCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
HWYDGAWGYFPTYTLGAMTAAQVFDAASRAVPDIGPAIGRGDFTPLLGWLRTHIHAKGCL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
LSTDALLREATGRPLDASVFKAHLTRRYLEDR
EHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTRSSPAAVSPSTAYAALERRFARLRAIEDSIAFLHWDQSAVMPAGGASSRADQLATLA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHH
GLHHDLLTAPDTAEMIALADPLAEAESAEDGAWRLANLAEITRIHAHAMALPGDLVEALS
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RAGNACERVWREARPLGDFALVEGPLGQLLGLVRDSAAAKAEALGVAPYDALLDQYEPGG
HHCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
SSARIDGIFDDLEAFLPDFLGRVIERQGAAAEAPAGPFPIPTQQAVGRATMESLGFDFDH
CCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
GRLDVSLHPFCGGSPQDLRLTTRYDEADFTSALMGVIHETGHALYEQGLPRDRLSQPVGR
CEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHH
ARGMSVHESQSLLFEMQAGRSRAFISHCAPAWKAAFGGEGEAWRADSLYRHFTRVEPGFI
HCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEE
RVDADEVTYPAHVIVRYRLEKALIEGRMALGDLPTAWNDAMERLLGVRPPNDRLGCLQDI
EECCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
HWYDGAWGYFPTYTLGAMTAAQVFDAASRAVPDIGPAIGRGDFTPLLGWLRTHIHAKGCL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
LSTDALLREATGRPLDASVFKAHLTRRYLEDR
EHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765282; 1369078 [H]