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The map label for this gene is vapI [H]

Identifier: 83594398

GI number: 83594398

Start: 3533712

End: 3533999

Strand: Reverse

Name: vapI [H]

Synonym: Rru_A3068

Alternate gene names: 83594398

Gene position: 3533999-3533712 (Counterclockwise)

Preceding gene: 83594399

Following gene: 83594392

Centisome position: 81.19

GC content: 65.97

Gene sequence:

>288_bases
GTGGCGCTGCCGCCCCTGCATCCGGGTGAATTTCTGGCCGATGAACTAACCGAGATCGGCGTATCGGTATCGGCGCTGGC
GCGCGCCCTTGATGTACCCCAGAGCCGCATGGCCGATATCGTCGCCGGCAAGCGATCGGTTACCGCCGACACGGCTCTGC
GCCTTGCCGCCTATTTCGGCACATCGGCCCGGCTGTGGCTGAACCTCCAGGCGGCTTACGACCTTGCGATCACCCAAGCC
AAAGAAGGCGCCCATATCGCCTCCGTCGTCCGTCCCCGGGCGGCCTGA

Upstream 100 bases:

>100_bases
GGCCAGTTCAGCATCCGCATCAACGACCAATGGCGGATTTGCTTCACCTTCCACGAGGGAGATGCCTATGACGCCGAAAT
CGTCGATTATCATTGAGGCC

Downstream 100 bases:

>100_bases
TGGACGGCCGGTCCGCTGGCGGCTACGCTTGTGAACCCATCGGCTCCGTCTCCCTTGCCTGTGGGCGGGGGTGGCTTATC
TGCCGGGGTCGACCATCGCC

Product: plasmid maintenance system antidote protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 95; Mature: 94

Protein sequence:

>95_residues
MALPPLHPGEFLADELTEIGVSVSALARALDVPQSRMADIVAGKRSVTADTALRLAAYFGTSARLWLNLQAAYDLAITQA
KEGAHIASVVRPRAA

Sequences:

>Translated_95_residues
MALPPLHPGEFLADELTEIGVSVSALARALDVPQSRMADIVAGKRSVTADTALRLAAYFGTSARLWLNLQAAYDLAITQA
KEGAHIASVVRPRAA
>Mature_94_residues
ALPPLHPGEFLADELTEIGVSVSALARALDVPQSRMADIVAGKRSVTADTALRLAAYFGTSARLWLNLQAAYDLAITQAK
EGAHIASVVRPRAA

Specific function: Unknown

COG id: COG3093

COG function: function code R; Plasmid maintenance system antidote protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693133, Length=70, Percent_Identity=35.7142857142857, Blast_Score=59, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013430
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 10027; Mature: 9895

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPPLHPGEFLADELTEIGVSVSALARALDVPQSRMADIVAGKRSVTADTALRLAAYFG
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHC
TSARLWLNLQAAYDLAITQAKEGAHIASVVRPRAA
CCEEEEEEEHHHHHHHHHCCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
ALPPLHPGEFLADELTEIGVSVSALARALDVPQSRMADIVAGKRSVTADTALRLAAYFG
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHC
TSARLWLNLQAAYDLAITQAKEGAHIASVVRPRAA
CCEEEEEEEHHHHHHHHHCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8169216 [H]