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The map label for this gene is yogA [H]

Identifier: 83594393

GI number: 83594393

Start: 3528708

End: 3529991

Strand: Direct

Name: yogA [H]

Synonym: Rru_A3063

Alternate gene names: 83594393

Gene position: 3528708-3529991 (Clockwise)

Preceding gene: 83594390

Following gene: 83594394

Centisome position: 81.07

GC content: 61.92

Gene sequence:

>1284_bases
ATGACCACGTCGGCGGAAGTCATAGAACTCAATCCCGGCACTGGCCGGAAGGATCTTTACGAACTCGGTGAAATTCCGCC
GCTCGGCCACGTTCCCAAGTCTATGTACGCCTGGGTCATCCGCCGGGATCGCCATGGCGAACCCGAGAAGTCTTTCCAGG
TTGAAGTCGTTGAAACGCCAACTCTTGACAGCCACGACGTCTTGGTGATGGTGATGGCGGCCGGCGTCAACTACAACGGG
ATCTGGGCCGGATTGGGCCAGCCGATCAGCGTTTTCGACTCGCATAAGGCCGCTTATCACATCGCCGGTTCGGATGCGGC
GGGCATCGTCTGGGCCGTCGGCGCCAAGGTCAAGCGCTGGAAGGTCGGCGACGAGGTGGTCGTCCACTGCAATCAGACCG
ACGGCGACGACGAGGAATGCAATGGTGGCGATCCGATGTTCTCGCCGACCCAGCGCATCTGGGGCTATGAGACCCCCGAT
GGCTCCTTCGCCCAGTTCACCCGCGTGCAGTCCCAGCAGGTGATGGCCCGTCCGCGCCATCTGACCTGGGAGGAAAGTGC
CAGCTACGTGCTGGTTCTGGCCACCGCCTATCGCATGCTGTTCGGCCACCGCCCCCATGTGCTGCGCCCGGGTCACAACG
TGCTGATCTGGGGCGCCTCGGGCGGCCTGGGATCGATGGCGATCCAGCTGTGCGCCACGGCGGGCGCCAATGCCATCGGC
GTCATCTCCGATGAGACCAAGCGCGATTTCGTCATGAGCCTGGGCGCCAAGGGCGTGATCAACCGCAAGGATTTCAATTG
CTGGGGCCAATTGCCCACGGTCAATGGCGAGGGCTTCGACGCCTATATGAAAGAGGTGCGCAAGTTCGGCAAGGCGATCT
GGGACATCACCGGCAAGGGCAACGACGTTGATTTCGTGTTCGAACATCCGGGCGAGCAGACCTTCCCGGTCTCGTGCAAT
GTGGTCAAGCGCGGTGGCATGGTGGTGTTTTGCGCCGGCACCACCGGCTTCAACCTGACCTTCGACGCCCGCTTTGTGTG
GATGCGCCAGAAGCGCATTCAGGGCAGCCACTTCGCCAATCTGCTCCAGGCCTCGCAAGCCAACCAGTTGGTCATCGAGC
GGCGGATCGATCCGTGCATGAGCGAAGTGTTTTCCTGGGACGATATTCCCAAGGCCCACACCAAGATGTGGAAGAATCAG
CATAAGCCGGGGAATATGGCGGTGCTGGTCCAGGCCCATCGCCCGGGCCGCCGCACCTTGGAGGATTGCCGAGAGGAAGG
GTGA

Upstream 100 bases:

>100_bases
AGTGAAAGAGGCTATTCGCTATCTGAGTCTTCAGGGCAAGGCCGATACGAGACAAAAGAACTCGAAAGCGGCCTAGAGGT
TTCAACGCGGAGGATAGACC

Downstream 100 bases:

>100_bases
AGCCGGCCAGGGGGGCGTTCCCCCCCCTGCCGATCATGATCCGCCCTCCCGCTTCCTCCCCGCCCCGCGCAAGGGCCCAG
ACGAGGGAATGAGTCCGATG

Product: crotonyl-CoA reductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTTSAEVIELNPGTGRKDLYELGEIPPLGHVPKSMYAWVIRRDRHGEPEKSFQVEVVETPTLDSHDVLVMVMAAGVNYNG
IWAGLGQPISVFDSHKAAYHIAGSDAAGIVWAVGAKVKRWKVGDEVVVHCNQTDGDDEECNGGDPMFSPTQRIWGYETPD
GSFAQFTRVQSQQVMARPRHLTWEESASYVLVLATAYRMLFGHRPHVLRPGHNVLIWGASGGLGSMAIQLCATAGANAIG
VISDETKRDFVMSLGAKGVINRKDFNCWGQLPTVNGEGFDAYMKEVRKFGKAIWDITGKGNDVDFVFEHPGEQTFPVSCN
VVKRGGMVVFCAGTTGFNLTFDARFVWMRQKRIQGSHFANLLQASQANQLVIERRIDPCMSEVFSWDDIPKAHTKMWKNQ
HKPGNMAVLVQAHRPGRRTLEDCREEG

Sequences:

>Translated_427_residues
MTTSAEVIELNPGTGRKDLYELGEIPPLGHVPKSMYAWVIRRDRHGEPEKSFQVEVVETPTLDSHDVLVMVMAAGVNYNG
IWAGLGQPISVFDSHKAAYHIAGSDAAGIVWAVGAKVKRWKVGDEVVVHCNQTDGDDEECNGGDPMFSPTQRIWGYETPD
GSFAQFTRVQSQQVMARPRHLTWEESASYVLVLATAYRMLFGHRPHVLRPGHNVLIWGASGGLGSMAIQLCATAGANAIG
VISDETKRDFVMSLGAKGVINRKDFNCWGQLPTVNGEGFDAYMKEVRKFGKAIWDITGKGNDVDFVFEHPGEQTFPVSCN
VVKRGGMVVFCAGTTGFNLTFDARFVWMRQKRIQGSHFANLLQASQANQLVIERRIDPCMSEVFSWDDIPKAHTKMWKNQ
HKPGNMAVLVQAHRPGRRTLEDCREEG
>Mature_426_residues
TTSAEVIELNPGTGRKDLYELGEIPPLGHVPKSMYAWVIRRDRHGEPEKSFQVEVVETPTLDSHDVLVMVMAAGVNYNGI
WAGLGQPISVFDSHKAAYHIAGSDAAGIVWAVGAKVKRWKVGDEVVVHCNQTDGDDEECNGGDPMFSPTQRIWGYETPDG
SFAQFTRVQSQQVMARPRHLTWEESASYVLVLATAYRMLFGHRPHVLRPGHNVLIWGASGGLGSMAIQLCATAGANAIGV
ISDETKRDFVMSLGAKGVINRKDFNCWGQLPTVNGEGFDAYMKEVRKFGKAIWDITGKGNDVDFVFEHPGEQTFPVSCNV
VKRGGMVVFCAGTTGFNLTFDARFVWMRQKRIQGSHFANLLQASQANQLVIERRIDPCMSEVFSWDDIPKAHTKMWKNQH
KPGNMAVLVQAHRPGRRTLEDCREEG

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=377, Percent_Identity=21.2201591511936, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI13236495, Length=377, Percent_Identity=21.2201591511936, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI194239676, Length=264, Percent_Identity=24.6212121212121, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17507255, Length=388, Percent_Identity=25.5154639175258, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71988145, Length=301, Percent_Identity=24.5847176079734, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324486, Length=364, Percent_Identity=25, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323961, Length=301, Percent_Identity=25.2491694352159, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319621, Length=223, Percent_Identity=30.0448430493274, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323729, Length=207, Percent_Identity=29.4685990338164, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 47361; Mature: 47230

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSAEVIELNPGTGRKDLYELGEIPPLGHVPKSMYAWVIRRDRHGEPEKSFQVEVVETP
CCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHEEECCCCCCCCCEEEEEEECC
TLDSHDVLVMVMAAGVNYNGIWAGLGQPISVFDSHKAAYHIAGSDAAGIVWAVGAKVKRW
CCCCCCEEEEEEECCCCCCCEEECCCCCCEEECCCCEEEEEECCCCCCEEEEECCCEEEE
KVGDEVVVHCNQTDGDDEECNGGDPMFSPTQRIWGYETPDGSFAQFTRVQSQQVMARPRH
ECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
LTWEESASYVLVLATAYRMLFGHRPHVLRPGHNVLIWGASGGLGSMAIQLCATAGANAIG
CCCCCCCCEEEEHHHHHHHHHCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHCCCCEEE
VISDETKRDFVMSLGAKGVINRKDFNCWGQLPTVNGEGFDAYMKEVRKFGKAIWDITGKG
EECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCC
NDVDFVFEHPGEQTFPVSCNVVKRGGMVVFCAGTTGFNLTFDARFVWMRQKRIQGSHFAN
CCEEEEEECCCCEECCEEEEEEECCCEEEEEECCCCCEEEEEEEEEEEHHHHCCHHHHHH
LLQASQANQLVIERRIDPCMSEVFSWDDIPKAHTKMWKNQHKPGNMAVLVQAHRPGRRTL
HHHHCCCCHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCHHHH
EDCREEG
HHHHHCC
>Mature Secondary Structure 
TTSAEVIELNPGTGRKDLYELGEIPPLGHVPKSMYAWVIRRDRHGEPEKSFQVEVVETP
CCCCEEEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHEEECCCCCCCCCEEEEEEECC
TLDSHDVLVMVMAAGVNYNGIWAGLGQPISVFDSHKAAYHIAGSDAAGIVWAVGAKVKRW
CCCCCCEEEEEEECCCCCCCEEECCCCCCEEECCCCEEEEEECCCCCCEEEEECCCEEEE
KVGDEVVVHCNQTDGDDEECNGGDPMFSPTQRIWGYETPDGSFAQFTRVQSQQVMARPRH
ECCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
LTWEESASYVLVLATAYRMLFGHRPHVLRPGHNVLIWGASGGLGSMAIQLCATAGANAIG
CCCCCCCCEEEEHHHHHHHHHCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHCCCCEEE
VISDETKRDFVMSLGAKGVINRKDFNCWGQLPTVNGEGFDAYMKEVRKFGKAIWDITGKG
EECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCC
NDVDFVFEHPGEQTFPVSCNVVKRGGMVVFCAGTTGFNLTFDARFVWMRQKRIQGSHFAN
CCEEEEEECCCCEECCEEEEEEECCCEEEEEECCCCCEEEEEEEEEEEHHHHCCHHHHHH
LLQASQANQLVIERRIDPCMSEVFSWDDIPKAHTKMWKNQHKPGNMAVLVQAHRPGRRTL
HHHHCCCCHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCHHHH
EDCREEG
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]