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The map label for this gene is meaA [H]

Identifier: 83594392

GI number: 83594392

Start: 3526474

End: 3528489

Strand: Reverse

Name: meaA [H]

Synonym: Rru_A3062

Alternate gene names: 83594392

Gene position: 3528489-3526474 (Counterclockwise)

Preceding gene: 83594398

Following gene: 83594391

Centisome position: 81.06

GC content: 65.33

Gene sequence:

>2016_bases
ATGTCTGGTGATGCCGCCTCCACGCCCAAGCCCAAGCTGCAAACTCGGGATAAGCCCTGGCTTTTCCGAACCTATTCGGG
GCATTCCTCGGCCCGGGCGTCGAACGCCCTCTATCGAACGAATCTTGCGCGGGGTCAGACCGGGCTGTCCGTCGCCTTCG
ATCTTCCGACTCAGACCGGCTATGACAGCGACCATGTTCTGGCGCGCGGCGAGGTCGGCAAGGTCGGCGTGCCGATCAGC
CATCTGGGCGACATGCAAACCCTGTTCGAGGGGATTCCCCTTGAAAAGATGAACACCTCGATGACGATCAACGCTCCGGC
GCCGTGGCTGCTGGCGCTTTATGTCGCCACCGCCGAGCGCCAGGGCGCCGATCGCGCCGCCCTTCAGGGCACGGTGCAGA
ACGATCTGATCAAGGAATACCTGAGCCGGGGCACTTATATCTTCCCGCCCGAGCCCTCGCTGCGGCTGATCGGCGATGTC
ATCGCCTTCACCTATCGCGAGATTCCCAAATGGAATCCGACCAACGTCTGCTCCTATCACCTACAGGAAGCCGGGGCGAC
GCCCGAGCAGGAGCTGGCCTTCGCCCTGGCCACGGCGATCGCCGTGCTTGATACGGTGAAGGCCGGCGGTCAGGTACCCG
AAGCCGATTTCCCCGAGGTGGTCGGGCGCATCAGCTTTTTCGTCAACGCCGGCATCCGCTTCATCACCGAGATGTGCAAG
ATGCGGGCGTTCTGCGACCTGTGGGATGAAATCTGCCTGACGCGCTATGGCGTCACCGACGAGAAGTTCCGCCGCTTCCG
CTATGGGGTGCAGGTCAATTCCCTGGGGTTGACCGAACAGCAGCCCGAAAACAACGTCTATCGCATCTTGCTTGAGATGC
TGGCGGTGGTCTTGTCAAAGAACGCCCGGGCGCGCGCCGTGCAGCTTCCCGCCTGGAACGAGGCGCTGGGCCTGCCCCGG
CCCTGGGATCAGCAATGGTCGCTGCGCCTTCAGCAGATCGTCGCCTTCGAGACCGATCTGCTCGATTACGGCGATATCTT
CGACGGCTCGCGCGAAATCGCCGCCAAGGTTGAAGAGCTTAAAACCGCCGCCCGCGACGAATTGGCGCGCATCGCCGCCA
TGGGCGGGGCCGAGGCGGCGGTTGAAGCCGGCTACATGAAGCAGAAGCTGGTCGAGAGCAACAGCCGGCGCATCGGCGGC
ATCGAAAGCGGCGAGCAGATCGTCGTCGGCGTCAATAAATACACCGAAGGCGCCGATAGCCCGCTGGTCGGCGGCGACGG
GGCGATCATGACCGTCGATCCCGCCGTCGAGGCCGAGCAGATCGCCGCCCTTACGGCCTGGAAGGCGGCGCGCGACGCCA
AGGACGTGGGGGCGGCCCTGGATGGTTTACGCCGGGCGGCGGCGGAAGGCACCAATATCATGGAGCCGACCATCGCCTGC
GCCCATGCCGGGGTGACCACCGGCGAATGGTCGCAGACCCTGCGCGAGGTGTTTGGCGAATACCGGGCGCCGACCGGAGT
CGGGCGGGCGGCGGCCCAGCCCGAAGCCGGCGGCCGGCTGGCCGAGGTGCGCCTTCGGGTGGATGATCTGTCGACCCGGC
TGGGGCGGCGGCTGAAGATCCTGGTCGGCAAGCCCGGGCTGGATGGCCATTCCAACGGCGCCGAGCAGATCGCCGTGCGG
GCGCGGGATTCGGGGATGGAGGTGGTCTATGAGGGCATCCGCCTGACCCCGGCGCAGATCGTCAATGCCGCCCTTGAGGA
AAGCGTTCACGTCGTTGGGCTGTCGATCCTGTCGGGGTCGCATGTGCCGCTGGTCAGCGATGTGCTTGACCGCATGCGGG
CCGAGGGGCTGGCCGATATTCCGGTGATCGTCGGCGGCATCATTCCGCCCGAGGACGAAAAGATCCTGCTGGCCGCCGGC
GTCGCCCGCGTCTACACGCCCAAGGATTACGATATCACCGCGATCATGGCCGATATCGTCGGCATCGTCGATCTGACCAC
CCGTCAGGCGGCGTAA

Upstream 100 bases:

>100_bases
AATAGTTTCGTATGAACTAGGTGATGAACAACATAGTTGCGCGGAATCCCCGTTTTTGTCCGAATTTTCCGGGAAACTCC
GCCTCTTCAGGGAGATCGAC

Downstream 100 bases:

>100_bases
GTCTTCTCGAGAGGGCGGCCGGCGGCGCGGGGCCGGCCGTTTCCCATTCGCCAAGGGCTGTGGTATTCGTTCATACCCCG
TCCTTTTGGTGGTTTTCGTC

Product: methylmalonyl-CoA mutase

Products: succinyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MSGDAASTPKPKLQTRDKPWLFRTYSGHSSARASNALYRTNLARGQTGLSVAFDLPTQTGYDSDHVLARGEVGKVGVPIS
HLGDMQTLFEGIPLEKMNTSMTINAPAPWLLALYVATAERQGADRAALQGTVQNDLIKEYLSRGTYIFPPEPSLRLIGDV
IAFTYREIPKWNPTNVCSYHLQEAGATPEQELAFALATAIAVLDTVKAGGQVPEADFPEVVGRISFFVNAGIRFITEMCK
MRAFCDLWDEICLTRYGVTDEKFRRFRYGVQVNSLGLTEQQPENNVYRILLEMLAVVLSKNARARAVQLPAWNEALGLPR
PWDQQWSLRLQQIVAFETDLLDYGDIFDGSREIAAKVEELKTAARDELARIAAMGGAEAAVEAGYMKQKLVESNSRRIGG
IESGEQIVVGVNKYTEGADSPLVGGDGAIMTVDPAVEAEQIAALTAWKAARDAKDVGAALDGLRRAAAEGTNIMEPTIAC
AHAGVTTGEWSQTLREVFGEYRAPTGVGRAAAQPEAGGRLAEVRLRVDDLSTRLGRRLKILVGKPGLDGHSNGAEQIAVR
ARDSGMEVVYEGIRLTPAQIVNAALEESVHVVGLSILSGSHVPLVSDVLDRMRAEGLADIPVIVGGIIPPEDEKILLAAG
VARVYTPKDYDITAIMADIVGIVDLTTRQAA

Sequences:

>Translated_671_residues
MSGDAASTPKPKLQTRDKPWLFRTYSGHSSARASNALYRTNLARGQTGLSVAFDLPTQTGYDSDHVLARGEVGKVGVPIS
HLGDMQTLFEGIPLEKMNTSMTINAPAPWLLALYVATAERQGADRAALQGTVQNDLIKEYLSRGTYIFPPEPSLRLIGDV
IAFTYREIPKWNPTNVCSYHLQEAGATPEQELAFALATAIAVLDTVKAGGQVPEADFPEVVGRISFFVNAGIRFITEMCK
MRAFCDLWDEICLTRYGVTDEKFRRFRYGVQVNSLGLTEQQPENNVYRILLEMLAVVLSKNARARAVQLPAWNEALGLPR
PWDQQWSLRLQQIVAFETDLLDYGDIFDGSREIAAKVEELKTAARDELARIAAMGGAEAAVEAGYMKQKLVESNSRRIGG
IESGEQIVVGVNKYTEGADSPLVGGDGAIMTVDPAVEAEQIAALTAWKAARDAKDVGAALDGLRRAAAEGTNIMEPTIAC
AHAGVTTGEWSQTLREVFGEYRAPTGVGRAAAQPEAGGRLAEVRLRVDDLSTRLGRRLKILVGKPGLDGHSNGAEQIAVR
ARDSGMEVVYEGIRLTPAQIVNAALEESVHVVGLSILSGSHVPLVSDVLDRMRAEGLADIPVIVGGIIPPEDEKILLAAG
VARVYTPKDYDITAIMADIVGIVDLTTRQAA
>Mature_670_residues
SGDAASTPKPKLQTRDKPWLFRTYSGHSSARASNALYRTNLARGQTGLSVAFDLPTQTGYDSDHVLARGEVGKVGVPISH
LGDMQTLFEGIPLEKMNTSMTINAPAPWLLALYVATAERQGADRAALQGTVQNDLIKEYLSRGTYIFPPEPSLRLIGDVI
AFTYREIPKWNPTNVCSYHLQEAGATPEQELAFALATAIAVLDTVKAGGQVPEADFPEVVGRISFFVNAGIRFITEMCKM
RAFCDLWDEICLTRYGVTDEKFRRFRYGVQVNSLGLTEQQPENNVYRILLEMLAVVLSKNARARAVQLPAWNEALGLPRP
WDQQWSLRLQQIVAFETDLLDYGDIFDGSREIAAKVEELKTAARDELARIAAMGGAEAAVEAGYMKQKLVESNSRRIGGI
ESGEQIVVGVNKYTEGADSPLVGGDGAIMTVDPAVEAEQIAALTAWKAARDAKDVGAALDGLRRAAAEGTNIMEPTIACA
HAGVTTGEWSQTLREVFGEYRAPTGVGRAAAQPEAGGRLAEVRLRVDDLSTRLGRRLKILVGKPGLDGHSNGAEQIAVRA
RDSGMEVVYEGIRLTPAQIVNAALEESVHVVGLSILSGSHVPLVSDVLDRMRAEGLADIPVIVGGIIPPEDEKILLAAGV
ARVYTPKDYDITAIMADIVGIVDLTTRQAA

Specific function: Required for the assimilation of methanol and ethanol [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=644, Percent_Identity=36.0248447204969, Blast_Score=385, Evalue=1e-107,
Organism=Escherichia coli, GI1789284, Length=637, Percent_Identity=37.3626373626374, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25144500, Length=642, Percent_Identity=37.0716510903427, Blast_Score=410, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: 5.4.99.2

Molecular weight: Translated: 72520; Mature: 72389

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDAASTPKPKLQTRDKPWLFRTYSGHSSARASNALYRTNLARGQTGLSVAFDLPTQTG
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHCHHHHHHHHCCCCCCEEEEECCCCCC
YDSDHVLARGEVGKVGVPISHLGDMQTLFEGIPLEKMNTSMTINAPAPWLLALYVATAER
CCCCCEEECCCCCCCCCCHHHHCCHHHHHCCCCHHHCCCCEEECCCCHHHHHHHHHHHHH
QGADRAALQGTVQNDLIKEYLSRGTYIFPPEPSLRLIGDVIAFTYREIPKWNPTNVCSYH
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
LQEAGATPEQELAFALATAIAVLDTVKAGGQVPEADFPEVVGRISFFVNAGIRFITEMCK
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MRAFCDLWDEICLTRYGVTDEKFRRFRYGVQVNSLGLTEQQPENNVYRILLEMLAVVLSK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCC
NARARAVQLPAWNEALGLPRPWDQQWSLRLQQIVAFETDLLDYGDIFDGSREIAAKVEEL
CCCCEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KTAARDELARIAAMGGAEAAVEAGYMKQKLVESNSRRIGGIESGEQIVVGVNKYTEGADS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCC
PLVGGDGAIMTVDPAVEAEQIAALTAWKAARDAKDVGAALDGLRRAAAEGTNIMEPTIAC
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
AHAGVTTGEWSQTLREVFGEYRAPTGVGRAAAQPEAGGRLAEVRLRVDDLSTRLGRRLKI
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHCCEEEE
LVGKPGLDGHSNGAEQIAVRARDSGMEVVYEGIRLTPAQIVNAALEESVHVVGLSILSGS
EEECCCCCCCCCCHHHEEEEECCCCHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCC
HVPLVSDVLDRMRAEGLADIPVIVGGIIPPEDEKILLAAGVARVYTPKDYDITAIMADIV
CCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCEEEEECHHHHCCCCCCCHHHHHHHHH
GIVDLTTRQAA
HHHHHHHHCCC
>Mature Secondary Structure 
SGDAASTPKPKLQTRDKPWLFRTYSGHSSARASNALYRTNLARGQTGLSVAFDLPTQTG
CCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHCHHHHHHHHCCCCCCEEEEECCCCCC
YDSDHVLARGEVGKVGVPISHLGDMQTLFEGIPLEKMNTSMTINAPAPWLLALYVATAER
CCCCCEEECCCCCCCCCCHHHHCCHHHHHCCCCHHHCCCCEEECCCCHHHHHHHHHHHHH
QGADRAALQGTVQNDLIKEYLSRGTYIFPPEPSLRLIGDVIAFTYREIPKWNPTNVCSYH
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
LQEAGATPEQELAFALATAIAVLDTVKAGGQVPEADFPEVVGRISFFVNAGIRFITEMCK
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MRAFCDLWDEICLTRYGVTDEKFRRFRYGVQVNSLGLTEQQPENNVYRILLEMLAVVLSK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCC
NARARAVQLPAWNEALGLPRPWDQQWSLRLQQIVAFETDLLDYGDIFDGSREIAAKVEEL
CCCCEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KTAARDELARIAAMGGAEAAVEAGYMKQKLVESNSRRIGGIESGEQIVVGVNKYTEGADS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCC
PLVGGDGAIMTVDPAVEAEQIAALTAWKAARDAKDVGAALDGLRRAAAEGTNIMEPTIAC
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
AHAGVTTGEWSQTLREVFGEYRAPTGVGRAAAQPEAGGRLAEVRLRVDDLSTRLGRRLKI
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHCCEEEE
LVGKPGLDGHSNGAEQIAVRARDSGMEVVYEGIRLTPAQIVNAALEESVHVVGLSILSGS
EEECCCCCCCCCCHHHEEEEECCCCHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCC
HVPLVSDVLDRMRAEGLADIPVIVGGIIPPEDEKILLAAGVARVYTPKDYDITAIMADIV
CCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCEEEEECHHHHCCCCCCCHHHHHHHHH
GIVDLTTRQAA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-methylmalonyl-CoA

Specific reaction: (R)-methylmalonyl-CoA = succinyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]