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The map label for this gene is ycbU [H]

Identifier: 83594370

GI number: 83594370

Start: 3503624

End: 3504769

Strand: Reverse

Name: ycbU [H]

Synonym: Rru_A3040

Alternate gene names: 83594370

Gene position: 3504769-3503624 (Counterclockwise)

Preceding gene: 83594376

Following gene: 83594360

Centisome position: 80.52

GC content: 68.15

Gene sequence:

>1146_bases
ATGCTTTCGTGCCAGCGTGACCTCTTTGAGATCCCCCAGGATGTGGCTTACCTGAACGCCGCCTTCATGGGCCCCTTGAT
GACCGAGGTGGTCGCCGCCGGCCATGCCGGGGTGGCGGCCAAGGCCCGGCCCTGGGAAGTGGCGATCGACGCCTTCTTCG
ATCCGGTCGAAAAGGCGCGCGGCCTGTATGCCGGGCTGACCGGCGCCGATGTCGAGGGCATCGCCGTCGTTCCCTCAGTG
TCCTATGGCATCGCCGTGGCGGCGGCCAATCTGCCGCTGGCGGCGGGGAAGCGGGTGCTGGTTCTGGAAGAGCAGTTCCC
TTCCAATCTTTATTCGTGGCGTCGTCTGGCGACCGAGAACAACGCCGTCGTCCAGGTTGTCGCCCGCCCGGCCAACGGCA
ATTGGACCGAGGCCCTGCTCGGCGCCATCAAGCCGGGGGTCGCCATCGTCGCCTGTCCCCAGGCCCATTGGTCGGATGGC
TGCAAGATCGATCTGGTCGCCATTGGTGCCGCCTGCCGTGCGGTCGGGGCGGCCCTGGTCATCGACGGCACCCAGTCCTT
TGGCGCCATGCCCTTCGACACGGCGGCGGTCGATCCGGATTTCGCCGTCGCCGCCACCTATAAGTGGCTGCTTGGCCCCT
ATTCGCTGGGGTTCCTCTATGTGGCGCCGCGCCATCGCAACGGTCAGCCGCTGGAAGAGGGCTGGATCTGCCGCGAAGGT
AGCCGGGATTTTTCGCGGCTGGTCGATTACACCGAGAGCATGGACGCCGGGGCGCGGCGTTTCGATGTGGGCGAACGCTC
GAACTTCGCCCTGATGCCGATGGCGATCGCCGCCATGGAGCGCCTGACCGCCTGGACGCCCGCCGCCGTATCGGCCTATG
CCGGGCGGCTGACCGACCGGGTGGTCGCCGAAACGGCGGCCTGGGGCTGCACCGCCGCCCCCGCTAGCGCCCGCTCGCCC
CATTTGCTGGGGTTGGGTTTGCCGGAAGGGGTTGACGCCAAGGCCTTGGCGACCCGGCTGGCCGCCGCCCAGGTCAGCGT
CAGCGTGCGCGGCAGCCGCCTGCGCATCTCGCCCCACGTCTATAACACCGACGCCGATGTCGACCGCCTGCTTGGCGTTC
TTGAAGACGCGCTGGCAAAGGCCTGA

Upstream 100 bases:

>100_bases
TGTTACCGTGACAATCTACCGATTGCACCGGGGCAATACCAGGGCAATCGTGGGGAATGCCTTTGGGCGTCCCCCCTCTC
CTGTCTTGCGAGCGTGACCG

Downstream 100 bases:

>100_bases
GCCTTTCCCAAAAAAGCGGGCAAGACAGCTTGTGGGGTGTTTTGACGCCAGACAGACGAAAAGACCCGGAGCGCGGCTTG
GCGGTCTCTCTCCGGGTCGA

Product: aminotransferase, class V

Products: L-alanine; S [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MLSCQRDLFEIPQDVAYLNAAFMGPLMTEVVAAGHAGVAAKARPWEVAIDAFFDPVEKARGLYAGLTGADVEGIAVVPSV
SYGIAVAAANLPLAAGKRVLVLEEQFPSNLYSWRRLATENNAVVQVVARPANGNWTEALLGAIKPGVAIVACPQAHWSDG
CKIDLVAIGAACRAVGAALVIDGTQSFGAMPFDTAAVDPDFAVAATYKWLLGPYSLGFLYVAPRHRNGQPLEEGWICREG
SRDFSRLVDYTESMDAGARRFDVGERSNFALMPMAIAAMERLTAWTPAAVSAYAGRLTDRVVAETAAWGCTAAPASARSP
HLLGLGLPEGVDAKALATRLAAAQVSVSVRGSRLRISPHVYNTDADVDRLLGVLEDALAKA

Sequences:

>Translated_381_residues
MLSCQRDLFEIPQDVAYLNAAFMGPLMTEVVAAGHAGVAAKARPWEVAIDAFFDPVEKARGLYAGLTGADVEGIAVVPSV
SYGIAVAAANLPLAAGKRVLVLEEQFPSNLYSWRRLATENNAVVQVVARPANGNWTEALLGAIKPGVAIVACPQAHWSDG
CKIDLVAIGAACRAVGAALVIDGTQSFGAMPFDTAAVDPDFAVAATYKWLLGPYSLGFLYVAPRHRNGQPLEEGWICREG
SRDFSRLVDYTESMDAGARRFDVGERSNFALMPMAIAAMERLTAWTPAAVSAYAGRLTDRVVAETAAWGCTAAPASARSP
HLLGLGLPEGVDAKALATRLAAAQVSVSVRGSRLRISPHVYNTDADVDRLLGVLEDALAKA
>Mature_381_residues
MLSCQRDLFEIPQDVAYLNAAFMGPLMTEVVAAGHAGVAAKARPWEVAIDAFFDPVEKARGLYAGLTGADVEGIAVVPSV
SYGIAVAAANLPLAAGKRVLVLEEQFPSNLYSWRRLATENNAVVQVVARPANGNWTEALLGAIKPGVAIVACPQAHWSDG
CKIDLVAIGAACRAVGAALVIDGTQSFGAMPFDTAAVDPDFAVAATYKWLLGPYSLGFLYVAPRHRNGQPLEEGWICREG
SRDFSRLVDYTESMDAGARRFDVGERSNFALMPMAIAAMERLTAWTPAAVSAYAGRLTDRVVAETAAWGCTAAPASARSP
HLLGLGLPEGVDAKALATRLAAAQVSVSVRGSRLRISPHVYNTDADVDRLLGVLEDALAKA

Specific function: Fe-S cluster biosynthesis [C]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 40222; Mature: 40222

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSCQRDLFEIPQDVAYLNAAFMGPLMTEVVAAGHAGVAAKARPWEVAIDAFFDPVEKAR
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHCHHHHHC
GLYAGLTGADVEGIAVVPSVSYGIAVAAANLPLAAGKRVLVLEEQFPSNLYSWRRLATEN
CEEECCCCCCCCCEEEECCCCCCEEEEECCCCHHCCCEEEEEECCCCCHHHHHHHHCCCC
NAVVQVVARPANGNWTEALLGAIKPGVAIVACPQAHWSDGCKIDLVAIGAACRAVGAALV
CEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECHHHHHCCEEEE
IDGTQSFGAMPFDTAAVDPDFAVAATYKWLLGPYSLGFLYVAPRHRNGQPLEEGWICREG
EECCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHCCEECCC
SRDFSRLVDYTESMDAGARRFDVGERSNFALMPMAIAAMERLTAWTPAAVSAYAGRLTDR
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VVAETAAWGCTAAPASARSPHLLGLGLPEGVDAKALATRLAAAQVSVSVRGSRLRISPHV
HHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEEEECCCEEEECCEE
YNTDADVDRLLGVLEDALAKA
ECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSCQRDLFEIPQDVAYLNAAFMGPLMTEVVAAGHAGVAAKARPWEVAIDAFFDPVEKAR
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHCHHHHHC
GLYAGLTGADVEGIAVVPSVSYGIAVAAANLPLAAGKRVLVLEEQFPSNLYSWRRLATEN
CEEECCCCCCCCCEEEECCCCCCEEEEECCCCHHCCCEEEEEECCCCCHHHHHHHHCCCC
NAVVQVVARPANGNWTEALLGAIKPGVAIVACPQAHWSDGCKIDLVAIGAACRAVGAALV
CEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECHHHHHCCEEEE
IDGTQSFGAMPFDTAAVDPDFAVAATYKWLLGPYSLGFLYVAPRHRNGQPLEEGWICREG
EECCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHCCEECCC
SRDFSRLVDYTESMDAGARRFDVGERSNFALMPMAIAAMERLTAWTPAAVSAYAGRLTDR
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VVAETAAWGCTAAPASARSPHLLGLGLPEGVDAKALATRLAAAQVSVSVRGSRLRISPHV
HHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEEEECCCEEEECCEE
YNTDADVDRLLGVLEDALAKA
ECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cysteine [C]

Specific reaction: L-cysteine = L-alanine + S [C]

General reaction: transfers sulfane sulfur to ThiI during in vitro biosynthesis of 4-thiouridine in E. coli tRNA [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377; 7704254; 1353026 [H]