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The map label for this gene is pncA [H]

Identifier: 83594332

GI number: 83594332

Start: 3459252

End: 3459857

Strand: Direct

Name: pncA [H]

Synonym: Rru_A3002

Alternate gene names: 83594332

Gene position: 3459252-3459857 (Clockwise)

Preceding gene: 83594322

Following gene: 83594333

Centisome position: 79.47

GC content: 67.16

Gene sequence:

>606_bases
ATGACCGACGCCCTGGTGCTGATCGATATCCAGAACGACTTCTGTCCCGGTGGTGCCCTGGCCGTTCCCGAAGGCGATCG
GGTGGTGGCGGTGGCCAACCGGCTGGCGCCGATGTTCGGCACGGTGATCCTCAGCCAGGATTGGCACCCGGCCGATCACC
GCTCGTTTGTCACCGCCCATCCCGGCAAGGCCGCCTTCGAGTCCGTGACCATGGACTACGGCCCCCAGGTCTTGTGGCCG
CCCCATTGCGTGGCCGGAACCCGGGGCGCGGCCTTTGTCGACGGCCTGGATCTCGGCCCGGCCCATGTTATCGTGCGCAA
GGGCACCAACCGCGACACCGACAGCTATTCGGCCTTCCAGGAAAACGACAAGCGCACCAGCACCGGTCTGGCCGGGCTGT
TGCGCGAACGCGGCATCGAGCGGATCTTCCTGGCCGGATTGGCGACGGACTTCTGCGTCTGTTACAGCGCCCTGGACGCC
CGCGCCCTGGGCTTCGAGGTCTGTCTGGTCGAAGACGGCTGCCGGGCCATCGATCTTGACGGATCGCTGGACCTTGCCCG
GGCGAAGATGGCGGCGGCCGGGGTGAAGATCGTCACCAGCCCCTGA

Upstream 100 bases:

>100_bases
GGACGACGACCCGCCTCGCGGGTGACGCTGCCCCGCTGACCGATTAAACTGTGGGGCCCGCTCCGCCGTCCCGGCCCCGC
TTGTTCCTGAAAGGACCGCC

Downstream 100 bases:

>100_bases
TCGCCGCCTCGGTCGGGCGCCCCGGCGCGCCTTGCCACCCCCCGCCGGCGCCGCTATGCTGGCCAAAAGCCGGGGCAAAG
GCTGCACGGCTCACGAAATC

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MTDALVLIDIQNDFCPGGALAVPEGDRVVAVANRLAPMFGTVILSQDWHPADHRSFVTAHPGKAAFESVTMDYGPQVLWP
PHCVAGTRGAAFVDGLDLGPAHVIVRKGTNRDTDSYSAFQENDKRTSTGLAGLLRERGIERIFLAGLATDFCVCYSALDA
RALGFEVCLVEDGCRAIDLDGSLDLARAKMAAAGVKIVTSP

Sequences:

>Translated_201_residues
MTDALVLIDIQNDFCPGGALAVPEGDRVVAVANRLAPMFGTVILSQDWHPADHRSFVTAHPGKAAFESVTMDYGPQVLWP
PHCVAGTRGAAFVDGLDLGPAHVIVRKGTNRDTDSYSAFQENDKRTSTGLAGLLRERGIERIFLAGLATDFCVCYSALDA
RALGFEVCLVEDGCRAIDLDGSLDLARAKMAAAGVKIVTSP
>Mature_200_residues
TDALVLIDIQNDFCPGGALAVPEGDRVVAVANRLAPMFGTVILSQDWHPADHRSFVTAHPGKAAFESVTMDYGPQVLWPP
HCVAGTRGAAFVDGLDLGPAHVIVRKGTNRDTDSYSAFQENDKRTSTGLAGLLRERGIERIFLAGLATDFCVCYSALDAR
ALGFEVCLVEDGCRAIDLDGSLDLARAKMAAAGVKIVTSP

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=202, Percent_Identity=45.049504950495, Blast_Score=164, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI193206735, Length=186, Percent_Identity=31.1827956989247, Blast_Score=93, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17543130, Length=221, Percent_Identity=28.0542986425339, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71999339, Length=223, Percent_Identity=30.9417040358744, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321401, Length=213, Percent_Identity=28.169014084507, Blast_Score=105, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648247, Length=200, Percent_Identity=34.5, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 21360; Mature: 21228

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDALVLIDIQNDFCPGGALAVPEGDRVVAVANRLAPMFGTVILSQDWHPADHRSFVTAH
CCCEEEEEECCCCCCCCCEEECCCCCEEEEHHHHHHHHHHEEEEECCCCCCCCCEEEEEC
PGKAAFESVTMDYGPQVLWPPHCVAGTRGAAFVDGLDLGPAHVIVRKGTNRDTDSYSAFQ
CCCHHHHHEECCCCCCEECCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHH
ENDKRTSTGLAGLLRERGIERIFLAGLATDFCVCYSALDARALGFEVCLVEDGCRAIDLD
HCCCHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEECC
GSLDLARAKMAAAGVKIVTSP
CCCHHHHHHHHHCCEEEEECC
>Mature Secondary Structure 
TDALVLIDIQNDFCPGGALAVPEGDRVVAVANRLAPMFGTVILSQDWHPADHRSFVTAH
CCEEEEEECCCCCCCCCEEECCCCCEEEEHHHHHHHHHHEEEEECCCCCCCCCEEEEEC
PGKAAFESVTMDYGPQVLWPPHCVAGTRGAAFVDGLDLGPAHVIVRKGTNRDTDSYSAFQ
CCCHHHHHEECCCCCCEECCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHH
ENDKRTSTGLAGLLRERGIERIFLAGLATDFCVCYSALDARALGFEVCLVEDGCRAIDLD
HCCCHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEECC
GSLDLARAKMAAAGVKIVTSP
CCCHHHHHHHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]