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The map label for this gene is pflB [H]

Identifier: 83594330

GI number: 83594330

Start: 3454293

End: 3456551

Strand: Reverse

Name: pflB [H]

Synonym: Rru_A3000

Alternate gene names: 83594330

Gene position: 3456551-3454293 (Counterclockwise)

Preceding gene: 83594331

Following gene: 83594329

Centisome position: 79.41

GC content: 63.52

Gene sequence:

>2259_bases
ATGAGTATCGTGCAAAAGGACATTCCCGCCACCAACCCCTGGAAAGGCTTTACCGAGGGCCCGTGGCAGACCGGCATCGA
TGTCCGTGATTTCATCCAGAAAAACGTGACCCCCTATCAGGGTGACGGCGCCTTCCTGGCCGGGCCGACCGACCGAACCC
GCCTGTTGTGGGAAAAACTGTCCGCGCTGTTGCTCAAGGAGCGCGAGAAGGGGGTGCTTGACGTTTCGGCCGACCGCGCC
TCGTCGATCACCGCCCATGACGCCGGCTATATCGACCGCGACGCCGAGATCATCGTCGGCCTGCAGACCGACGCCCCGCT
CAAGCGCGCCATCATGCCCAACGGCGGCCTGCGCATGGTTCAAAGCGGCCTGGAGGCCTATGGCTTCGCCAGCGATCCCA
CCGTCAACGAGGTGTGGACCAAATACCGCAAAAGCCACAACCAGGGCATCTTCGACGTTTACAGCCCCGATGTTCTGGCC
GCCCGCAAATCGGGCGTGATCACCGGCCTGCCCGACGCCTATGGCCGGGGCCGCATCATCGGCGACTATCGCCGCGTCGC
CCTCTATGGCGTCGATTTCCTGCGCGACCAGAAGCAAATGGAATTCCACGAGCTTGATGACGCGGTGTTCAGCGAGGACG
TCATCCGCCTGCGCGAGGAACTGTCCGAGCAGTGGCGGGCGCTCGATGAGCTCAAGCAGATGGCGGCCAAATACGGCCAT
GGCATCGGTCGCCCAGCGGCCAATGCCCGCGAAGCCATCCAGTGGACCTATTTCGCCTATCTGGCGGCGGTCAAGGAGCA
GAACGGCGCGGCGATGTCGCTGGGCCGGGTGTCGAGCTTCCTTGATATCTACATCCAGCGCGATATCGAAGCCGGCCTTC
TCAGCGAAGACGGCGCCCAGGAGATGATCGACGATCTAGTGATCAAGCTGCGCATCGTCCGCTTCCTGCGCACCCCGGAC
TACGATCAGCTGTTCTCGGGCGATCCGACCTGGGTCACCGAATGCGTCGGCGGCATGGCCGAGGATGGCCGTACCCTGGT
CACCCGCAACAGCTTCCGCTTCCTCAATACCCTTTACAACCTGGGTCCGGCCCCCGAGCCCAATCTGACCGTGCTGTGGA
GCACGGCCCTGCCGCAGAGCTTCAAGACCTTCTGCGCCAAGGTTTCGGCCGATACCAGCGCCATTCAATACGAGAATGAC
GACCTGATGCGCCGGCGCTGGGGCGATGATTACGGCATCGCCTGCTGCGTGTCGGCCATGCGCATCGGCAAGCAGATGCA
GTTCTTCGGCGCCCGCGCCAATCTGGCCAAGGCCCTGCTCTACGCCATCAACGGCGGCGTCGATGAAAAATCCGGTCAGG
TGGTGGCCAAGGGCTTCGCGCCGATCACCGCCGATGTCCTCGACTATGACGAGGTGATGGCCAAGCTCGACAAGACCATG
GACTGGCTGGCCCAGACCTATGTCAAGGCGCTCAACACCATCCACTACATGCACGACAAATACGCCTATGAGCGGATCGA
GATGGCGCTGCATGACCGCGACATCCTGCGCACCATGGCCTGCGGCATCGCCGGGCTGTCGGTGGCGGCCGATTCGCTTT
CGGCCATCAAGTTCGCCAAGGTCCATGTCGTGCGCGACGACAAGGGGCTGGCCGTCGATTACACGATCGAGGGCGACTAT
CCCGCCTATGGCAACAACGACGACCGCGCCGATTCCATCGCCGTCTGGCTGACCGAAACCTTCATGGAAAAGGTCCGCGC
CCAGCCGCGCTTCTACCGCGACGCGATGCCGACCCAATCGGTTCTGACCATCACCTCGAACGTGGTTTACGGCAAGAAGA
CCGGCAACACCCCCGACGGCCGCCGCGCCGGCGAGCCCTTCGCGCCGGGTGCCAACCCGATGAACGGCCGCGATGTCAAA
GGCTTCGTCGCCTCGGGCGCCTCGGTCGCCAAGCTGCCCTATGCCGCCGCCCTCGATGGCATCAGCTGGACCGCGTCGGC
CACCCCGGATTCGCTCGGACGCACCGACGAGGAGCGGGCGGTCAATCTGTCGAATTGCCTGGACGCCTTCGCCACCGCCG
GCGGCTTCCACGTCAACGTCAACGTCTTCAACCGCGACACCTTGCTCCACGCCATGGACCACCCGGAGCTTTACCCGCAG
TTGACCATCCGCGTGTCGGGCTACGCCGTGAATTTCGTCAAGCTGACCCGTGAACAGCAGCTCGACGTTATCGGCCGCAC
CTTCCACCAGACCATGTGA

Upstream 100 bases:

>100_bases
CTCCCACACCTTGTGACAAAGGCGCCGCCGCCGGTGGCGCCTTCCCCGACCAGGAACCGCTTTCCCCCGATTTTCGGTTA
TCCGCTCATAGGAGCTTCTG

Downstream 100 bases:

>100_bases
TCCATCCCCTTTCCCGGGCCGCCGGTCGGCCCGGGATTTCGAACGGTGCGACGGGCCGGCAAGCCTCCCCGGTCCGTCGC
AGGAAGAGGACAAACCGATG

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 752; Mature: 751

Protein sequence:

>752_residues
MSIVQKDIPATNPWKGFTEGPWQTGIDVRDFIQKNVTPYQGDGAFLAGPTDRTRLLWEKLSALLLKEREKGVLDVSADRA
SSITAHDAGYIDRDAEIIVGLQTDAPLKRAIMPNGGLRMVQSGLEAYGFASDPTVNEVWTKYRKSHNQGIFDVYSPDVLA
ARKSGVITGLPDAYGRGRIIGDYRRVALYGVDFLRDQKQMEFHELDDAVFSEDVIRLREELSEQWRALDELKQMAAKYGH
GIGRPAANAREAIQWTYFAYLAAVKEQNGAAMSLGRVSSFLDIYIQRDIEAGLLSEDGAQEMIDDLVIKLRIVRFLRTPD
YDQLFSGDPTWVTECVGGMAEDGRTLVTRNSFRFLNTLYNLGPAPEPNLTVLWSTALPQSFKTFCAKVSADTSAIQYEND
DLMRRRWGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGVDEKSGQVVAKGFAPITADVLDYDEVMAKLDKTM
DWLAQTYVKALNTIHYMHDKYAYERIEMALHDRDILRTMACGIAGLSVAADSLSAIKFAKVHVVRDDKGLAVDYTIEGDY
PAYGNNDDRADSIAVWLTETFMEKVRAQPRFYRDAMPTQSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMNGRDVK
GFVASGASVAKLPYAAALDGISWTASATPDSLGRTDEERAVNLSNCLDAFATAGGFHVNVNVFNRDTLLHAMDHPELYPQ
LTIRVSGYAVNFVKLTREQQLDVIGRTFHQTM

Sequences:

>Translated_752_residues
MSIVQKDIPATNPWKGFTEGPWQTGIDVRDFIQKNVTPYQGDGAFLAGPTDRTRLLWEKLSALLLKEREKGVLDVSADRA
SSITAHDAGYIDRDAEIIVGLQTDAPLKRAIMPNGGLRMVQSGLEAYGFASDPTVNEVWTKYRKSHNQGIFDVYSPDVLA
ARKSGVITGLPDAYGRGRIIGDYRRVALYGVDFLRDQKQMEFHELDDAVFSEDVIRLREELSEQWRALDELKQMAAKYGH
GIGRPAANAREAIQWTYFAYLAAVKEQNGAAMSLGRVSSFLDIYIQRDIEAGLLSEDGAQEMIDDLVIKLRIVRFLRTPD
YDQLFSGDPTWVTECVGGMAEDGRTLVTRNSFRFLNTLYNLGPAPEPNLTVLWSTALPQSFKTFCAKVSADTSAIQYEND
DLMRRRWGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGVDEKSGQVVAKGFAPITADVLDYDEVMAKLDKTM
DWLAQTYVKALNTIHYMHDKYAYERIEMALHDRDILRTMACGIAGLSVAADSLSAIKFAKVHVVRDDKGLAVDYTIEGDY
PAYGNNDDRADSIAVWLTETFMEKVRAQPRFYRDAMPTQSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMNGRDVK
GFVASGASVAKLPYAAALDGISWTASATPDSLGRTDEERAVNLSNCLDAFATAGGFHVNVNVFNRDTLLHAMDHPELYPQ
LTIRVSGYAVNFVKLTREQQLDVIGRTFHQTM
>Mature_751_residues
SIVQKDIPATNPWKGFTEGPWQTGIDVRDFIQKNVTPYQGDGAFLAGPTDRTRLLWEKLSALLLKEREKGVLDVSADRAS
SITAHDAGYIDRDAEIIVGLQTDAPLKRAIMPNGGLRMVQSGLEAYGFASDPTVNEVWTKYRKSHNQGIFDVYSPDVLAA
RKSGVITGLPDAYGRGRIIGDYRRVALYGVDFLRDQKQMEFHELDDAVFSEDVIRLREELSEQWRALDELKQMAAKYGHG
IGRPAANAREAIQWTYFAYLAAVKEQNGAAMSLGRVSSFLDIYIQRDIEAGLLSEDGAQEMIDDLVIKLRIVRFLRTPDY
DQLFSGDPTWVTECVGGMAEDGRTLVTRNSFRFLNTLYNLGPAPEPNLTVLWSTALPQSFKTFCAKVSADTSAIQYENDD
LMRRRWGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGVDEKSGQVVAKGFAPITADVLDYDEVMAKLDKTMD
WLAQTYVKALNTIHYMHDKYAYERIEMALHDRDILRTMACGIAGLSVAADSLSAIKFAKVHVVRDDKGLAVDYTIEGDYP
AYGNNDDRADSIAVWLTETFMEKVRAQPRFYRDAMPTQSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMNGRDVKG
FVASGASVAKLPYAAALDGISWTASATPDSLGRTDEERAVNLSNCLDAFATAGGFHVNVNVFNRDTLLHAMDHPELYPQL
TIRVSGYAVNFVKLTREQQLDVIGRTFHQTM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=750, Percent_Identity=64.5333333333333, Blast_Score=1011, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=750, Percent_Identity=62.6666666666667, Blast_Score=955, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=575, Percent_Identity=27.304347826087, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1790388, Length=647, Percent_Identity=26.5842349304482, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788933, Length=59, Percent_Identity=66.1016949152542, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 83406; Mature: 83275

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVQKDIPATNPWKGFTEGPWQTGIDVRDFIQKNVTPYQGDGAFLAGPTDRTRLLWEKL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHH
SALLLKEREKGVLDVSADRASSITAHDAGYIDRDAEIIVGLQTDAPLKRAIMPNGGLRMV
HHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCCHHHH
QSGLEAYGFASDPTVNEVWTKYRKSHNQGIFDVYSPDVLAARKSGVITGLPDAYGRGRII
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCEECCCCCCCCCCEE
GDYRRVALYGVDFLRDQKQMEFHELDDAVFSEDVIRLREELSEQWRALDELKQMAAKYGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIGRPAANAREAIQWTYFAYLAAVKEQNGAAMSLGRVSSFLDIYIQRDIEAGLLSEDGAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHH
EMIDDLVIKLRIVRFLRTPDYDQLFSGDPTWVTECVGGMAEDGRTLVTRNSFRFLNTLYN
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHCCCCCCCCEEEECCHHHHHHHHHH
LGPAPEPNLTVLWSTALPQSFKTFCAKVSADTSAIQYENDDLMRRRWGDDYGIACCVSAM
CCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCHHHHHHHHH
RIGKQMQFFGARANLAKALLYAINGGVDEKSGQVVAKGFAPITADVLDYDEVMAKLDKTM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
DWLAQTYVKALNTIHYMHDKYAYERIEMALHDRDILRTMACGIAGLSVAADSLSAIKFAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
VHVVRDDKGLAVDYTIEGDYPAYGNNDDRADSIAVWLTETFMEKVRAQPRFYRDAMPTQS
EEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
VLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMNGRDVKGFVASGASVAKLPYAAALDG
EEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCHHHHHCC
ISWTASATPDSLGRTDEERAVNLSNCLDAFATAGGFHVNVNVFNRDTLLHAMDHPELYPQ
CCEECCCCCHHCCCCCHHHHCCHHHHHHHHHCCCCEEEEEEEECCCHHHHCCCCCCCCCE
LTIRVSGYAVNFVKLTREQQLDVIGRTFHQTM
EEEEEECEEEEEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIVQKDIPATNPWKGFTEGPWQTGIDVRDFIQKNVTPYQGDGAFLAGPTDRTRLLWEKL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHH
SALLLKEREKGVLDVSADRASSITAHDAGYIDRDAEIIVGLQTDAPLKRAIMPNGGLRMV
HHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCCHHHH
QSGLEAYGFASDPTVNEVWTKYRKSHNQGIFDVYSPDVLAARKSGVITGLPDAYGRGRII
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCEECCCCCCCCCCEE
GDYRRVALYGVDFLRDQKQMEFHELDDAVFSEDVIRLREELSEQWRALDELKQMAAKYGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIGRPAANAREAIQWTYFAYLAAVKEQNGAAMSLGRVSSFLDIYIQRDIEAGLLSEDGAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHH
EMIDDLVIKLRIVRFLRTPDYDQLFSGDPTWVTECVGGMAEDGRTLVTRNSFRFLNTLYN
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHCCCCCCCCEEEECCHHHHHHHHHH
LGPAPEPNLTVLWSTALPQSFKTFCAKVSADTSAIQYENDDLMRRRWGDDYGIACCVSAM
CCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCHHHHHHHHH
RIGKQMQFFGARANLAKALLYAINGGVDEKSGQVVAKGFAPITADVLDYDEVMAKLDKTM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
DWLAQTYVKALNTIHYMHDKYAYERIEMALHDRDILRTMACGIAGLSVAADSLSAIKFAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
VHVVRDDKGLAVDYTIEGDYPAYGNNDDRADSIAVWLTETFMEKVRAQPRFYRDAMPTQS
EEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
VLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMNGRDVKGFVASGASVAKLPYAAALDG
EEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCHHHHHCC
ISWTASATPDSLGRTDEERAVNLSNCLDAFATAGGFHVNVNVFNRDTLLHAMDHPELYPQ
CCEECCCCCHHCCCCCHHHHCCHHHHHHHHHCCCCEEEEEEEECCCHHHHCCCCCCCCCE
LTIRVSGYAVNFVKLTREQQLDVIGRTFHQTM
EEEEEECEEEEEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA