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The map label for this gene is ydgK [H]

Identifier: 83594301

GI number: 83594301

Start: 3421204

End: 3422436

Strand: Reverse

Name: ydgK [H]

Synonym: Rru_A2971

Alternate gene names: 83594301

Gene position: 3422436-3421204 (Counterclockwise)

Preceding gene: 83594304

Following gene: 83594293

Centisome position: 78.63

GC content: 66.67

Gene sequence:

>1233_bases
ATGCCCCAGCTTTCCCCGCCCACCGATCGCCATCGCGAGATCGGATTTCCCGAGTTCGTCGGCTTGATGGCGATGCTGAT
GGCGCTGACGGCGTTGTCGATCGACGTCATGCTGGTTGCCCTGGCCGATATCTCGTCGGACTACTTTTTGGCCGATGCCA
ATGACCGCCAGTTGGTCATCACCGCCTATCTGCTGGGGTTCGCCGTCGGCCAGCCGGTCTGGGGGCCGCTGTCGGACCGC
ATCGGCCGCAAGCCGGTGCTGATCGCCGGGCTGGTGATCTATGGCCTGGGCTGCCTGGGCGCCATGGTGTCGCCCAGCTT
CGAAACCCTGCTCGCCGCCCGGCTGGTCCAGGGACTGGGCGGCGCCTCGCCCCGGGTGATGACCATGGCCGTGGTCCGCG
ACCGCTATGCCGGCAGTGGCATGGCCCGGGTGATGTCGCTGTTGATGATGATCTTCATCATCGTGCCGATCCTGGCGCCG
ACCATGGGCAGCGCGGTGATGGCCTTCGCCCCCTGGCACTGGCTGTTCGGCTTCATGATGGTTTTCGATCTCATCGTGCT
GGTCTGGACCCTGACCCGGCTGCGGGAAACCCACCGGCCCCAGCGCCAACTCGCCCCGTCCTTGGGCGGGCTGCTGGAAA
CCCTGCGTGTCGTCGTCAGCAACCGCCAGACGGTGGGCTATACGGTGGCCTTCGGCTTCATGATGGGCAGTCTGATGGGC
TATGTCGGCACCGCCCAGCAGGTTTTCGTCGAGGTTTATCACGAGGTCGAGCTGTTCCCGATCCTCTTTGGCGCCATCGC
CTCGGCCATCGCCGCCGCCTCGATCATCAACGCCCGTTTCGTGACGCGGATCGGCTCGCGGCGGATGTCCCATGCCGCCT
TGCTGGGGTTCGTCGCCGTCGGGCTGGTGACGGCGGCCTTCGGCTTCCCGGCCAAGCCGCCGCTTGCCCTGTTCATGGCG
GCCCTGGGCATCCAGTTCTTTTGCTTCGGCCTGATCGGGCCGAATTTCAACGCCATGGCGATGGAGCCGATGGGCCGGGT
CGCCGGGGCCGCCTCGTCGTTCGTCGGCTTTTACTCCACCGCCGCCAGCGCCTTTCTGGGATGGGCGGTCGGCCAGCAGT
TCGACGACAGCGTCCGGCCGCTGATGATGGGCTTCGCCGGCTTTGGCATTCTCGCCCTGGTCTGCGTGCTGGTCACCGAG
CGCGGCCGGCTGTTCCGTCGCGCCGCCGCCTAA

Upstream 100 bases:

>100_bases
GGCTCTTTGGATCTGCGGGCTTTCAACGGCGAAGCGTGTTGCCTATGTTTGGCCTGCCGCGTCCCCCGGCGCGCCTTGAA
AACCCTGCCCCGGATCCCCC

Downstream 100 bases:

>100_bases
CCTTTCGCCTCGTCCTCGGGCTTGCGCAGGATATAGGTGTCCATGATCCAGCCCTTGGCTTGGCGCGCCCGCTCGCGGCG
GGCGAGGATCGCCTCGGAGG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MPQLSPPTDRHREIGFPEFVGLMAMLMALTALSIDVMLVALADISSDYFLADANDRQLVITAYLLGFAVGQPVWGPLSDR
IGRKPVLIAGLVIYGLGCLGAMVSPSFETLLAARLVQGLGGASPRVMTMAVVRDRYAGSGMARVMSLLMMIFIIVPILAP
TMGSAVMAFAPWHWLFGFMMVFDLIVLVWTLTRLRETHRPQRQLAPSLGGLLETLRVVVSNRQTVGYTVAFGFMMGSLMG
YVGTAQQVFVEVYHEVELFPILFGAIASAIAAASIINARFVTRIGSRRMSHAALLGFVAVGLVTAAFGFPAKPPLALFMA
ALGIQFFCFGLIGPNFNAMAMEPMGRVAGAASSFVGFYSTAASAFLGWAVGQQFDDSVRPLMMGFAGFGILALVCVLVTE
RGRLFRRAAA

Sequences:

>Translated_410_residues
MPQLSPPTDRHREIGFPEFVGLMAMLMALTALSIDVMLVALADISSDYFLADANDRQLVITAYLLGFAVGQPVWGPLSDR
IGRKPVLIAGLVIYGLGCLGAMVSPSFETLLAARLVQGLGGASPRVMTMAVVRDRYAGSGMARVMSLLMMIFIIVPILAP
TMGSAVMAFAPWHWLFGFMMVFDLIVLVWTLTRLRETHRPQRQLAPSLGGLLETLRVVVSNRQTVGYTVAFGFMMGSLMG
YVGTAQQVFVEVYHEVELFPILFGAIASAIAAASIINARFVTRIGSRRMSHAALLGFVAVGLVTAAFGFPAKPPLALFMA
ALGIQFFCFGLIGPNFNAMAMEPMGRVAGAASSFVGFYSTAASAFLGWAVGQQFDDSVRPLMMGFAGFGILALVCVLVTE
RGRLFRRAAA
>Mature_409_residues
PQLSPPTDRHREIGFPEFVGLMAMLMALTALSIDVMLVALADISSDYFLADANDRQLVITAYLLGFAVGQPVWGPLSDRI
GRKPVLIAGLVIYGLGCLGAMVSPSFETLLAARLVQGLGGASPRVMTMAVVRDRYAGSGMARVMSLLMMIFIIVPILAPT
MGSAVMAFAPWHWLFGFMMVFDLIVLVWTLTRLRETHRPQRQLAPSLGGLLETLRVVVSNRQTVGYTVAFGFMMGSLMGY
VGTAQQVFVEVYHEVELFPILFGAIASAIAAASIINARFVTRIGSRRMSHAALLGFVAVGLVTAAFGFPAKPPLALFMAA
LGIQFFCFGLIGPNFNAMAMEPMGRVAGAASSFVGFYSTAASAFLGWAVGQQFDDSVRPLMMGFAGFGILALVCVLVTER
GRLFRRAAA

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=402, Percent_Identity=26.3681592039801, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI48994889, Length=186, Percent_Identity=33.8709677419355, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790146, Length=221, Percent_Identity=31.2217194570136, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790794, Length=192, Percent_Identity=32.8125, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787065, Length=276, Percent_Identity=27.1739130434783, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082312, Length=337, Percent_Identity=28.7833827893175, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790195, Length=175, Percent_Identity=29.7142857142857, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44031; Mature: 43900

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PQLSPPTDRHREIGFPEFVGLMAMLMALTALSIDVMLVALADISSDYFLADANDRQLVI
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEHH
TAYLLGFAVGQPVWGPLSDRIGRKPVLIAGLVIYGLGCLGAMVSPSFETLLAARLVQGLG
HHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
GASPRVMTMAVVRDRYAGSGMARVMSLLMMIFIIVPILAPTMGSAVMAFAPWHWLFGFMM
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
VFDLIVLVWTLTRLRETHRPQRQLAPSLGGLLETLRVVVSNRQTVGYTVAFGFMMGSLMG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
YVGTAQQVFVEVYHEVELFPILFGAIASAIAAASIINARFVTRIGSRRMSHAALLGFVAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVTAAFGFPAKPPLALFMAALGIQFFCFGLIGPNFNAMAMEPMGRVAGAASSFVGFYST
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHH
AASAFLGWAVGQQFDDSVRPLMMGFAGFGILALVCVLVTERGRLFRRAAA
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]