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The map label for this gene is ispB [H]

Identifier: 83594282

GI number: 83594282

Start: 3399890

End: 3400972

Strand: Reverse

Name: ispB [H]

Synonym: Rru_A2950

Alternate gene names: 83594282

Gene position: 3400972-3399890 (Counterclockwise)

Preceding gene: 83594289

Following gene: 83594281

Centisome position: 78.13

GC content: 65.37

Gene sequence:

>1083_bases
ATGCTCCGCCTGACTCCAGGGGGGGTCCGTCGCCATCATCAGGGATCAGGTAAGGCCTTGGCCAACGTCGTCAATCACGA
AAAACAGCGCGAAGGGCGGGAAGACGCCCTTGATGTGCTTCTCACACTGGTCGGCGGCGACCTGAAAAGGGTCAATGAGA
TCATCGTCGAACGGATGCACAGTCCGATCGCCCTGATCCCGCAACTGGCCGGCCATATCATCGCCGCCGGCGGCAAACGC
CTGCGGCCGCTGCTCACCCTCGCCTCGGCCCGCCTGTGCGGCTACGAGGGCGAGCGCCACGCCCAGCTCGCCGCCTGCGT
CGAATTCATCCATACCGCCACCTTGTTGCATGACGACGTGGTCGATGAAAGCCTGTTGCGCCGGGGCCGCGACAGCGCCA
ACGCCCTGTGGGGCAACAAGGCCAGCGTGCTGGTCGGCGACTTCCTGTTCTCGCGCTCCTTCGAACTGATGGTCGAGGAC
GGGGTGCTCAAGGTCTTGGGCATCTTGTCCCACGCCAGCCGGGTGATCGCCGAGGGCGAGGTTGCCCAGCTGATCACCGC
CAATGACATGGCGACGACCGAGGCCCAGTACCTTGAGGTCATCCGCGGCAAGACCGCCCAGTTGTTCGCCGCCGCCTGCC
GCATCGGCGCCGTCGTCGCCGAACGCCCGGAGGCCGAGGAAGAGGCGCTTGACACCTATGGCATGACCCTGGGTATCGCC
TTCCAGCTGGTCGATGACTATCTGGACTATTCGGCCCGCCAGCAGGATCTGGGCAAGACGGTCGGCGATGATTTCATGGA
CGGCAAGATCACCTTGCCCGTCATCCTGGCCTATCAGCGCGGCACCGACGACGAGCGCGCCTTCTGGCGCCGCTGCATGG
AAGCCCAGGACATGGCCGAGCCCGAGAAGGATCTGGCCAAGGCCATCGAGCTGATGACCCGCTACGGCACCCTGGCCGAG
ACCGTGGAACGCGCCCGCCACTACGGCGAACGCGCCCGCTCGGCCCTGGACATCTTCGCCGATGGCCCGGCCAAGGCGGC
TTTGGCCGGCATTGTCGATTTCTGCATCGAACGGGCCCGATAA

Upstream 100 bases:

>100_bases
CGTTCAAAGGGCGCCGGTCAACCCAAGGGAAAAGCGGCGAACCCCATTACTTTCGCGCGACCCGGGCCTGCTCGCTTGAC
CCGAGCGGCTGGGCGCTCCT

Downstream 100 bases:

>100_bases
GGCGCGTTTTCTGCTTGCGCCCCCCGGGCCGGGGGGCTATAACCCCGGCCCTCGGACGGAGTGTAGCTCAGCCTGGTAGA
GCACTGTCTTCGGGAGGCAG

Product: farnesyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MLRLTPGGVRRHHQGSGKALANVVNHEKQREGREDALDVLLTLVGGDLKRVNEIIVERMHSPIALIPQLAGHIIAAGGKR
LRPLLTLASARLCGYEGERHAQLAACVEFIHTATLLHDDVVDESLLRRGRDSANALWGNKASVLVGDFLFSRSFELMVED
GVLKVLGILSHASRVIAEGEVAQLITANDMATTEAQYLEVIRGKTAQLFAAACRIGAVVAERPEAEEEALDTYGMTLGIA
FQLVDDYLDYSARQQDLGKTVGDDFMDGKITLPVILAYQRGTDDERAFWRRCMEAQDMAEPEKDLAKAIELMTRYGTLAE
TVERARHYGERARSALDIFADGPAKAALAGIVDFCIERAR

Sequences:

>Translated_360_residues
MLRLTPGGVRRHHQGSGKALANVVNHEKQREGREDALDVLLTLVGGDLKRVNEIIVERMHSPIALIPQLAGHIIAAGGKR
LRPLLTLASARLCGYEGERHAQLAACVEFIHTATLLHDDVVDESLLRRGRDSANALWGNKASVLVGDFLFSRSFELMVED
GVLKVLGILSHASRVIAEGEVAQLITANDMATTEAQYLEVIRGKTAQLFAAACRIGAVVAERPEAEEEALDTYGMTLGIA
FQLVDDYLDYSARQQDLGKTVGDDFMDGKITLPVILAYQRGTDDERAFWRRCMEAQDMAEPEKDLAKAIELMTRYGTLAE
TVERARHYGERARSALDIFADGPAKAALAGIVDFCIERAR
>Mature_360_residues
MLRLTPGGVRRHHQGSGKALANVVNHEKQREGREDALDVLLTLVGGDLKRVNEIIVERMHSPIALIPQLAGHIIAAGGKR
LRPLLTLASARLCGYEGERHAQLAACVEFIHTATLLHDDVVDESLLRRGRDSANALWGNKASVLVGDFLFSRSFELMVED
GVLKVLGILSHASRVIAEGEVAQLITANDMATTEAQYLEVIRGKTAQLFAAACRIGAVVAERPEAEEEALDTYGMTLGIA
FQLVDDYLDYSARQQDLGKTVGDDFMDGKITLPVILAYQRGTDDERAFWRRCMEAQDMAEPEKDLAKAIELMTRYGTLAE
TVERARHYGERARSALDIFADGPAKAALAGIVDFCIERAR

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=319, Percent_Identity=29.153605015674, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4758430, Length=226, Percent_Identity=22.5663716814159, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI83700220, Length=226, Percent_Identity=22.5663716814159, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789578, Length=327, Percent_Identity=45.2599388379205, Blast_Score=277, Evalue=7e-76,
Organism=Escherichia coli, GI1786623, Length=226, Percent_Identity=30.9734513274336, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=234, Percent_Identity=36.3247863247863, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319475, Length=312, Percent_Identity=31.0897435897436, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325188, Length=226, Percent_Identity=25.6637168141593, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24651612, Length=301, Percent_Identity=31.5614617940199, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281365769, Length=241, Percent_Identity=24.0663900414938, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24660002, Length=241, Percent_Identity=24.0663900414938, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 39505; Mature: 39505

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLTPGGVRRHHQGSGKALANVVNHEKQREGREDALDVLLTLVGGDLKRVNEIIVERMH
CCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHC
SPIALIPQLAGHIIAAGGKRLRPLLTLASARLCGYEGERHAQLAACVEFIHTATLLHDDV
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VDESLLRRGRDSANALWGNKASVLVGDFLFSRSFELMVEDGVLKVLGILSHASRVIAEGE
HHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
VAQLITANDMATTEAQYLEVIRGKTAQLFAAACRIGAVVAERPEAEEEALDTYGMTLGIA
HHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FQLVDDYLDYSARQQDLGKTVGDDFMDGKITLPVILAYQRGTDDERAFWRRCMEAQDMAE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
PEKDLAKAIELMTRYGTLAETVERARHYGERARSALDIFADGPAKAALAGIVDFCIERAR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLRLTPGGVRRHHQGSGKALANVVNHEKQREGREDALDVLLTLVGGDLKRVNEIIVERMH
CCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHC
SPIALIPQLAGHIIAAGGKRLRPLLTLASARLCGYEGERHAQLAACVEFIHTATLLHDDV
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VDESLLRRGRDSANALWGNKASVLVGDFLFSRSFELMVEDGVLKVLGILSHASRVIAEGE
HHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
VAQLITANDMATTEAQYLEVIRGKTAQLFAAACRIGAVVAERPEAEEEALDTYGMTLGIA
HHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FQLVDDYLDYSARQQDLGKTVGDDFMDGKITLPVILAYQRGTDDERAFWRRCMEAQDMAE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
PEKDLAKAIELMTRYGTLAETVERARHYGERARSALDIFADGPAKAALAGIVDFCIERAR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]