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The map label for this gene is yafC [H]

Identifier: 83594231

GI number: 83594231

Start: 3341485

End: 3342453

Strand: Direct

Name: yafC [H]

Synonym: Rru_A2899

Alternate gene names: 83594231

Gene position: 3341485-3342453 (Clockwise)

Preceding gene: 83594230

Following gene: 83594233

Centisome position: 76.77

GC content: 62.75

Gene sequence:

>969_bases
GTGGTTTGGGGAGGGAGCCCCGGATCGGCCAAAGCGGCGAGCGGAAAACGGGGCATGGCGCAACGGCCGGGCAATATGGA
TTGGGATAAGCTGCGCGTCTTCCACTCGGTGGCGGAAGCGGGCAGTTTCACCCACGCCGGGGAAGCCCTCAATCTCAGTC
AATCGGCGGTCAGCCGTCAAATCAGCGCCCTGGAGGAAAGCCTCAAGGTGGCGCTGTTCCATCGCCACGCCCGGGGTCTG
ATCCTGACCGAACAGGGCGAGTTGCTGTTTGATACGGTTCACGATGTCTTTGGCAAGCTCGCCACCGTCGAGGCCAAGCT
GGGCGAGACCAAGGAGCGGCCGGAAGGGCCGCTGACCATCACCACCACCTTGTCCTTTGGTGCCATCTGGTTGACGCCGC
GCATCAAGCGCTTCATGGACCGCTATCCCGATATCGATGTCACCTTGGCGCTCAATGATACCGAGCTGGATCTGGCGATG
CGTCAGGCCGACGTGGCCCTGCGCTTCATGCCGCCGCGCCAGCCCGACCTGATCCAGCGCCAGCTTTTCAGCATGCCCTA
TCATGTCTTCGCCTCGCCCGATTACCTGAAGAAGCACGGCACGCCCCGGCGGGTGGAGGATCTCGACGATCACGCCCTTG
TCGTCTATGGCGAGGAGTTCAAGCCGCCGGTCGCCAATATGAGCTGGCTGCTGACGGCGGGCACCCGCGAGGGGCGGGCG
CGCAAGCCGATCCTTAAGGTCAACAACATCTATGGGATGTTCCGCGCCGTCGAAAGCGGCCTGGGCATCGCCGCCCTGCC
CGATTACTTCTCGAGCGAGGCGTCGACTCTGGTCAAGATCCTCCCCGAGGAACACGGCCCCGAGATGCAGATCTTCTTTG
TCTATGCCGAGGAATTGCGCCACAGCAAGCGGGTCGCGGTCTTCCGCGATTTTCTGATCAAGGAACTGGGCAGTTCCGGT
CTGGCCTGA

Upstream 100 bases:

>100_bases
TCCTGGCTTTGACGCAGGAATGACAAGACCGGCAAGCCGACCGCGAAGGGGCGTTCCACGCCCTTTTGGCGGTTTGGCCT
GCTGACAGGGATGGACCGGC

Downstream 100 bases:

>100_bases
CCCCCGCGAACCACCCGATCAAAAGACTGATTTTGAACACTTTTCCCAGGGCTGCGGGAAAAGGGCCATAGCCATGCGCC
GACCGCATGGCCTGCTTGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MVWGGSPGSAKAASGKRGMAQRPGNMDWDKLRVFHSVAEAGSFTHAGEALNLSQSAVSRQISALEESLKVALFHRHARGL
ILTEQGELLFDTVHDVFGKLATVEAKLGETKERPEGPLTITTTLSFGAIWLTPRIKRFMDRYPDIDVTLALNDTELDLAM
RQADVALRFMPPRQPDLIQRQLFSMPYHVFASPDYLKKHGTPRRVEDLDDHALVVYGEEFKPPVANMSWLLTAGTREGRA
RKPILKVNNIYGMFRAVESGLGIAALPDYFSSEASTLVKILPEEHGPEMQIFFVYAEELRHSKRVAVFRDFLIKELGSSG
LA

Sequences:

>Translated_322_residues
MVWGGSPGSAKAASGKRGMAQRPGNMDWDKLRVFHSVAEAGSFTHAGEALNLSQSAVSRQISALEESLKVALFHRHARGL
ILTEQGELLFDTVHDVFGKLATVEAKLGETKERPEGPLTITTTLSFGAIWLTPRIKRFMDRYPDIDVTLALNDTELDLAM
RQADVALRFMPPRQPDLIQRQLFSMPYHVFASPDYLKKHGTPRRVEDLDDHALVVYGEEFKPPVANMSWLLTAGTREGRA
RKPILKVNNIYGMFRAVESGLGIAALPDYFSSEASTLVKILPEEHGPEMQIFFVYAEELRHSKRVAVFRDFLIKELGSSG
LA
>Mature_322_residues
MVWGGSPGSAKAASGKRGMAQRPGNMDWDKLRVFHSVAEAGSFTHAGEALNLSQSAVSRQISALEESLKVALFHRHARGL
ILTEQGELLFDTVHDVFGKLATVEAKLGETKERPEGPLTITTTLSFGAIWLTPRIKRFMDRYPDIDVTLALNDTELDLAM
RQADVALRFMPPRQPDLIQRQLFSMPYHVFASPDYLKKHGTPRRVEDLDDHALVVYGEEFKPPVANMSWLLTAGTREGRA
RKPILKVNNIYGMFRAVESGLGIAALPDYFSSEASTLVKILPEEHGPEMQIFFVYAEELRHSKRVAVFRDFLIKELGSSG
LA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=33.6805555555556, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=28.0276816608997, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787589, Length=279, Percent_Identity=28.673835125448, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789440, Length=287, Percent_Identity=26.8292682926829, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=27.3037542662116, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787128, Length=192, Percent_Identity=31.25, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789173, Length=186, Percent_Identity=34.9462365591398, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789639, Length=238, Percent_Identity=27.3109243697479, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI157672245, Length=150, Percent_Identity=34.6666666666667, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788706, Length=186, Percent_Identity=29.5698924731183, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786448, Length=155, Percent_Identity=32.258064516129, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790262, Length=254, Percent_Identity=27.1653543307087, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790011, Length=254, Percent_Identity=23.2283464566929, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788748, Length=254, Percent_Identity=26.3779527559055, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=258, Percent_Identity=29.8449612403101, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=120, Percent_Identity=29.1666666666667, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI2367136, Length=260, Percent_Identity=25, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35892; Mature: 35892

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWGGSPGSAKAASGKRGMAQRPGNMDWDKLRVFHSVAEAGSFTHAGEALNLSQSAVSRQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
ISALEESLKVALFHRHARGLILTEQGELLFDTVHDVFGKLATVEAKLGETKERPEGPLTI
HHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
TTTLSFGAIWLTPRIKRFMDRYPDIDVTLALNDTELDLAMRQADVALRFMPPRQPDLIQR
EEEECCCEEEECHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCEEEEECCCCCHHHHHH
QLFSMPYHVFASPDYLKKHGTPRRVEDLDDHALVVYGEEFKPPVANMSWLLTAGTREGRA
HHHHCCEEEECCCHHHHHCCCCCHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCC
RKPILKVNNIYGMFRAVESGLGIAALPDYFSSEASTLVKILPEEHGPEMQIFFVYAEELR
CCCEEEHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHH
HSKRVAVFRDFLIKELGSSGLA
CHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVWGGSPGSAKAASGKRGMAQRPGNMDWDKLRVFHSVAEAGSFTHAGEALNLSQSAVSRQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
ISALEESLKVALFHRHARGLILTEQGELLFDTVHDVFGKLATVEAKLGETKERPEGPLTI
HHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
TTTLSFGAIWLTPRIKRFMDRYPDIDVTLALNDTELDLAMRQADVALRFMPPRQPDLIQR
EEEECCCEEEECHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCEEEEECCCCCHHHHHH
QLFSMPYHVFASPDYLKKHGTPRRVEDLDDHALVVYGEEFKPPVANMSWLLTAGTREGRA
HHHHCCEEEECCCHHHHHCCCCCHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCC
RKPILKVNNIYGMFRAVESGLGIAALPDYFSSEASTLVKILPEEHGPEMQIFFVYAEELR
CCCEEEHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHH
HSKRVAVFRDFLIKELGSSGLA
CHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]