Click here to switch to the map view.

The map label for this gene is greA

Identifier: 83594219

GI number: 83594219

Start: 3332082

End: 3332555

Strand: Direct

Name: greA

Synonym: Rru_A2887

Alternate gene names: 83594219

Gene position: 3332082-3332555 (Clockwise)

Preceding gene: 83594218

Following gene: 83594220

Centisome position: 76.55

GC content: 62.03

Gene sequence:

>474_bases
ATGGAGAAGATTCCCATGACTCCCGACGGGCTTGCCCGCCTGGAGGCAGAATTGAAGTCTTTGAAGTCCGTGGACCGGCC
AGCGGTGATCCGTGCGATCAGCGAAGCCCGTGACCACGGCGACCTTTCGGAGAATGCCGAATACCACGCCGCCCGTGAAC
GCCAGAGCTTCATCGAGGGCCGAATCAAGGAACTCGAGGATGTGACCTCGCGGGCCGAGGTGATCGACATCAGCAAGCTG
AGCGGCGATACCGTGCGCTTCGGCGCGACCGTCAGCGTGCTTGATGAGGACAGCGAGGAACAGACCACCTATCAGATCGT
CGGTGCCCACGAAGCCGACCTCAAGGCCGGGCGGATCAGCGTGGCCTCGCCGATCGGCAAGGCGCTGATCGGCAAAAAGG
TCGGCGATTCCATCGAGGTCAAGGCCCCGGGTGGCTCGAAGTTCTATGAAGTGACGATGGTCCGCTTCGGCTGA

Upstream 100 bases:

>100_bases
TTTAAAGTTTCCTGACCGCCGCAGGGGGGATTAGGCGAAGCGTCGCGTGGCGGGGTGCCGCGCGACGGCATGGACCATTG
TATTTTAAGGATCCGTTCCC

Downstream 100 bases:

>100_bases
CCCTCTCCCTCCCCTCCCTTCCCGCTCTCACCCCCGCTTCCGGCGGGGGTTTTTCTTTGCCCGCAGGCGGGCGGCGGGAT
TATCGCAGGCGTCCTCGGAG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MEKIPMTPDGLARLEAELKSLKSVDRPAVIRAISEARDHGDLSENAEYHAARERQSFIEGRIKELEDVTSRAEVIDISKL
SGDTVRFGATVSVLDEDSEEQTTYQIVGAHEADLKAGRISVASPIGKALIGKKVGDSIEVKAPGGSKFYEVTMVRFG

Sequences:

>Translated_157_residues
MEKIPMTPDGLARLEAELKSLKSVDRPAVIRAISEARDHGDLSENAEYHAARERQSFIEGRIKELEDVTSRAEVIDISKL
SGDTVRFGATVSVLDEDSEEQTTYQIVGAHEADLKAGRISVASPIGKALIGKKVGDSIEVKAPGGSKFYEVTMVRFG
>Mature_157_residues
MEKIPMTPDGLARLEAELKSLKSVDRPAVIRAISEARDHGDLSENAEYHAARERQSFIEGRIKELEDVTSRAEVIDISKL
SGDTVRFGATVSVLDEDSEEQTTYQIVGAHEADLKAGRISVASPIGKALIGKKVGDSIEVKAPGGSKFYEVTMVRFG

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=55.4140127388535, Blast_Score=166, Evalue=6e-43,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=31.6129032258064, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_RHORT (Q2RQB1)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_427971.1
- HSSP:   Q5SJG6
- ProteinModelPortal:   Q2RQB1
- SMR:   Q2RQB1
- STRING:   Q2RQB1
- GeneID:   3836328
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A2887
- NMPDR:   fig|1085.1.peg.532
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- PhylomeDB:   Q2RQB1
- ProtClustDB:   PRK00226
- BioCyc:   RRUB269796:RRU_A2887-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17058; Mature: 17058

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIPMTPDGLARLEAELKSLKSVDRPAVIRAISEARDHGDLSENAEYHAARERQSFIEG
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RIKELEDVTSRAEVIDISKLSGDTVRFGATVSVLDEDSEEQTTYQIVGAHEADLKAGRIS
HHHHHHHHHHHHHEEEHHHCCCCEEEECCEEEEECCCCCCCEEEEEECCCCCCCCCCCEE
VASPIGKALIGKKVGDSIEVKAPGGSKFYEVTMVRFG
HHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEC
>Mature Secondary Structure
MEKIPMTPDGLARLEAELKSLKSVDRPAVIRAISEARDHGDLSENAEYHAARERQSFIEG
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RIKELEDVTSRAEVIDISKLSGDTVRFGATVSVLDEDSEEQTTYQIVGAHEADLKAGRIS
HHHHHHHHHHHHHEEEHHHCCCCEEEECCEEEEECCCCCCCEEEEEECCCCCCCCCCCEE
VASPIGKALIGKKVGDSIEVKAPGGSKFYEVTMVRFG
HHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA