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The map label for this gene is livJ [C]

Identifier: 83594205

GI number: 83594205

Start: 3308792

End: 3309976

Strand: Reverse

Name: livJ [C]

Synonym: Rru_A2873

Alternate gene names: 83594205

Gene position: 3309976-3308792 (Counterclockwise)

Preceding gene: 83594207

Following gene: 83594204

Centisome position: 76.04

GC content: 67.09

Gene sequence:

>1185_bases
ATGAAAGTCTTCGGCCGGATTGGCCCGTTTTGCGCCTTGCTCGCCATGCTCGGGCTTGGTCTCGTTTCCCCCTCGGCCGC
CCGCGAGGATACGGGCGAGGGCGTTTCCCAGGAACCCGCCCTTCTTCTGGGCTACGAGTTGCCGCTGACCGGCGCCAATG
CGGCCTATGGTCGGGTCTTCCAGGAGGCGGCCCGCTTGCAGCTTGATCGCTTCAACGCCGCCGGTGGCGTCGGCGGCAGG
CCGGTCGACATCCTCTATGCCGATTCCCGCGATGACGCCGATCAGGCCCGCACCATCGCCCGCGCCTTCGTCGACGATCC
GCGGGTTGTCGGCGTGCTGGGCGATTTCTCCTCGACCGTCTCGATGGCGGCGGGATCGATCTATGGCAAGGAAGGCATGC
CCCAGCTGTCGCCGACCGCCGCCCATCCCGATTACATCAAGATCAGCCCCTGGCAGTTCCGCGCCATCACCACCCCGGCC
TTCGAAGGCCCCAATAACGCCGCCTGGATGATCGGCGACGGCTTCACCAGCGTCGCCGTGATCGGTGTCACCACCGATTG
GGGGCTTTCCTCGGCCCAGGCCTTCCGCAAGGCTTTCGAGCTGCGCGGCGGTGCGGTGGTGGTCAACGAAGAAGTCCCGC
CGGGCAACCGCCGCTTTGATGACGTGATCGACGAGATCGAGGATGAGGCCCCCCAGGCGATCTATCTGGCCATGGCCTAT
GAAGACGCCGCCCCCTTTCTGCGGGCGCTGCGCGCCCGGGGCAGCGCCCTGCCGGTCTATGGATCAAGCGCGCTGTATTC
GCCGAAATTCATCGATCTGGGGGGGCCGGCGGTCGAGGGGGTGCGCCTTGCCACCGCCTTCGTTCTCGGCGCGTCCGACC
CGGTGGTGGTCGAGTTCGTCAGCGCCTATGAAACCCTTTACGGCGCCATTCCCACCCTGTTCGCCGCCCACGGCTATGAC
GCCGTCGGCATCATGCTGGCGGCGGTCGGCCGGGCCGGCCCCGAGGTGACGCGCGAGAGCCTGCGCGACGCCCTTGCCGC
CACCGACCGCTACGCCGGGGTCACCGGGATCACCCGCTTTGATCCCGAAACCCGCGAAACCACCAAGATTCTGACCCGGC
TGGTGGTGCGCGAGGGCGATTTCCGGGTTATCGACCGCTCTGATTTCTCGCCGGTTCTTCCCTGA

Upstream 100 bases:

>100_bases
CGTTAGATCCTGGGGTTCCATGGGTCAGTAAACGACCGGCTTGTGGCGAGGGCGGGGCGGTCTGCGTTGTGCGATCCCCG
ATGATAGGGTTTCCCAGACG

Downstream 100 bases:

>100_bases
TCGGCCGTCGATCGCCAGTCAGAGCGTTTTCCCCCGTGGACGGAGGTCCAAGACGATCATGAGCATCAGTATTTTGGAAC
AACGGCGCATAGAAGCCGCT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKVFGRIGPFCALLAMLGLGLVSPSAAREDTGEGVSQEPALLLGYELPLTGANAAYGRVFQEAARLQLDRFNAAGGVGGR
PVDILYADSRDDADQARTIARAFVDDPRVVGVLGDFSSTVSMAAGSIYGKEGMPQLSPTAAHPDYIKISPWQFRAITTPA
FEGPNNAAWMIGDGFTSVAVIGVTTDWGLSSAQAFRKAFELRGGAVVVNEEVPPGNRRFDDVIDEIEDEAPQAIYLAMAY
EDAAPFLRALRARGSALPVYGSSALYSPKFIDLGGPAVEGVRLATAFVLGASDPVVVEFVSAYETLYGAIPTLFAAHGYD
AVGIMLAAVGRAGPEVTRESLRDALAATDRYAGVTGITRFDPETRETTKILTRLVVREGDFRVIDRSDFSPVLP

Sequences:

>Translated_394_residues
MKVFGRIGPFCALLAMLGLGLVSPSAAREDTGEGVSQEPALLLGYELPLTGANAAYGRVFQEAARLQLDRFNAAGGVGGR
PVDILYADSRDDADQARTIARAFVDDPRVVGVLGDFSSTVSMAAGSIYGKEGMPQLSPTAAHPDYIKISPWQFRAITTPA
FEGPNNAAWMIGDGFTSVAVIGVTTDWGLSSAQAFRKAFELRGGAVVVNEEVPPGNRRFDDVIDEIEDEAPQAIYLAMAY
EDAAPFLRALRARGSALPVYGSSALYSPKFIDLGGPAVEGVRLATAFVLGASDPVVVEFVSAYETLYGAIPTLFAAHGYD
AVGIMLAAVGRAGPEVTRESLRDALAATDRYAGVTGITRFDPETRETTKILTRLVVREGDFRVIDRSDFSPVLP
>Mature_394_residues
MKVFGRIGPFCALLAMLGLGLVSPSAAREDTGEGVSQEPALLLGYELPLTGANAAYGRVFQEAARLQLDRFNAAGGVGGR
PVDILYADSRDDADQARTIARAFVDDPRVVGVLGDFSSTVSMAAGSIYGKEGMPQLSPTAAHPDYIKISPWQFRAITTPA
FEGPNNAAWMIGDGFTSVAVIGVTTDWGLSSAQAFRKAFELRGGAVVVNEEVPPGNRRFDDVIDEIEDEAPQAIYLAMAY
EDAAPFLRALRARGSALPVYGSSALYSPKFIDLGGPAVEGVRLATAFVLGASDPVVVEFVSAYETLYGAIPTLFAAHGYD
AVGIMLAAVGRAGPEVTRESLRDALAATDRYAGVTGITRFDPETRETTKILTRLVVREGDFRVIDRSDFSPVLP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41864; Mature: 41864

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFGRIGPFCALLAMLGLGLVSPSAAREDTGEGVSQEPALLLGYELPLTGANAAYGRVF
CCCCHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
QEAARLQLDRFNAAGGVGGRPVDILYADSRDDADQARTIARAFVDDPRVVGVLGDFSSTV
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHH
SMAAGSIYGKEGMPQLSPTAAHPDYIKISPWQFRAITTPAFEGPNNAAWMIGDGFTSVAV
HHHHCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCCCCEEEEECCCCEEEE
IGVTTDWGLSSAQAFRKAFELRGGAVVVNEEVPPGNRRFDDVIDEIEDEAPQAIYLAMAY
EEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEEH
EDAAPFLRALRARGSALPVYGSSALYSPKFIDLGGPAVEGVRLATAFVLGASDPVVVEFV
HHHHHHHHHHHHCCCCCEEECCCCCCCCCEEECCCCHHHHHEEEEEEEECCCCCHHHHHH
SAYETLYGAIPTLFAAHGYDAVGIMLAAVGRAGPEVTRESLRDALAATDRYAGVTGITRF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
DPETRETTKILTRLVVREGDFRVIDRSDFSPVLP
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MKVFGRIGPFCALLAMLGLGLVSPSAAREDTGEGVSQEPALLLGYELPLTGANAAYGRVF
CCCCHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
QEAARLQLDRFNAAGGVGGRPVDILYADSRDDADQARTIARAFVDDPRVVGVLGDFSSTV
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHH
SMAAGSIYGKEGMPQLSPTAAHPDYIKISPWQFRAITTPAFEGPNNAAWMIGDGFTSVAV
HHHHCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCCCCEEEEECCCCEEEE
IGVTTDWGLSSAQAFRKAFELRGGAVVVNEEVPPGNRRFDDVIDEIEDEAPQAIYLAMAY
EEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEEH
EDAAPFLRALRARGSALPVYGSSALYSPKFIDLGGPAVEGVRLATAFVLGASDPVVVEFV
HHHHHHHHHHHHCCCCCEEECCCCCCCCCEEECCCCHHHHHEEEEEEEECCCCCHHHHHH
SAYETLYGAIPTLFAAHGYDAVGIMLAAVGRAGPEVTRESLRDALAATDRYAGVTGITRF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
DPETRETTKILTRLVVREGDFRVIDRSDFSPVLP
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA