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The map label for this gene is hipO [H]

Identifier: 83594203

GI number: 83594203

Start: 3307070

End: 3308245

Strand: Reverse

Name: hipO [H]

Synonym: Rru_A2871

Alternate gene names: 83594203

Gene position: 3308245-3307070 (Counterclockwise)

Preceding gene: 83594204

Following gene: 83594198

Centisome position: 76.0

GC content: 68.45

Gene sequence:

>1176_bases
ATGACCCCCGCCATCCCGCCCGCCATCGCGGCGCTGACCGGTGATATGAAAGCGTGGCGTCATCATCTGCACGCCCATCC
CGAAACCGCCTTCGAAGAGCACGCCACCGCCGATTTCATCGCCGGGCTGCTCGACGACTTCGGGGTCGAGGTCCATCGCG
GGCTGGCCGGAACCGGGGTGGTCGGGGTGATCGCCGGCAAACGGACGGGAAACCGCGCGATCGGGTTGCGCGCCGATATC
GACGCCCTGCACGTCACCGAGGCCACCGGCCTGCCCCACGCCTCGGTCCATGCCGGGCGCATGCACGCCTGCGGCCATGA
CGGCCACACGGCGATGCTGCTGGGAGCGGCCAAGCATCTGGCCGCGACCCGCGATTTCGCCGGCAGGCTGATCCTCATCT
TCCAGCCCGCCGAGGAAAACGAGGGCGGCGGCAAGGTGATGGTCGAAGAGGGCTTGTTCGACCGGTTCCCCGTGGATGCG
GTCTATGGCATGCACAACTGGCCGGGGCTGGAGGAAGGCCACTTCGCCCTGCGCACCGGTCCGATCATGGCCGGCTATGA
CGTGTTCGAGATCACGCTTACCGGCAAGGGGGGCCATGCCGCCATGCCCCATCTCGGCACCGATCAGTTGGTGGCGGCCG
GGCATCTGATGACCGCCTTGCAGTCGATCGTCGCCCGCTCGGTCAATCCGACCGAGGCGGCGGTGGTGTCGGTCACCCAG
ATGCACGGCGGCGACACCTGGAACGTCCTGCCCGCCAGCGTCGTGCTGCGTGGCACCGTGCGCACCTTCACCAAAGCCGT
GCAGGATCTGATCGAGACGCGGATCACCGAGCTGTCGCGATCGATCGCCCAGGGCTTTGGCGCCGAGGCGGCGATCCATT
ACGAGCGGCGCTATCCCGCCACCGTCAACAGCCCCGAGGAAGCCGCCGTCGCCGCCCGCGTGGCCAGCGCCGTGGTCGGC
GCCGACAAGGTGGACACCAATTGCCCGCAGACCATGGGGGCGGAGGATTTCGCCTTCATGCTGGGGGTCAAGCCGGGCGC
CTATGTGCAGCTTGGCGCCGGCCCGGGGCGGGGCGGTTGCATGCTCCACAACCCCGGTTACGACTTCAACGACGCCCTTC
TGGGCGTAGGGGCGAGCTATTGGGTGGGGCTGGTCCACGACCAACTGGCCGGCTAG

Upstream 100 bases:

>100_bases
ATCCCGATGCCACGATGGAACGGACCCAGACCATCATGAAGGGCGGACGCTGCTGCGATTTCCGCTATCACCTGAAGACG
ACCGATACGGAGAACGCGCA

Downstream 100 bases:

>100_bases
GCTTCTCGCGAGCCTCGGCGGCGAGGGGGTTAGGCCCCCTCGCCGTCGGTGTTCTCGAAGTGGAGACGCACCTTGTCGAT
GCTGCGGCCCTGCATCGAGA

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTPAIPPAIAALTGDMKAWRHHLHAHPETAFEEHATADFIAGLLDDFGVEVHRGLAGTGVVGVIAGKRTGNRAIGLRADI
DALHVTEATGLPHASVHAGRMHACGHDGHTAMLLGAAKHLAATRDFAGRLILIFQPAEENEGGGKVMVEEGLFDRFPVDA
VYGMHNWPGLEEGHFALRTGPIMAGYDVFEITLTGKGGHAAMPHLGTDQLVAAGHLMTALQSIVARSVNPTEAAVVSVTQ
MHGGDTWNVLPASVVLRGTVRTFTKAVQDLIETRITELSRSIAQGFGAEAAIHYERRYPATVNSPEEAAVAARVASAVVG
ADKVDTNCPQTMGAEDFAFMLGVKPGAYVQLGAGPGRGGCMLHNPGYDFNDALLGVGASYWVGLVHDQLAG

Sequences:

>Translated_391_residues
MTPAIPPAIAALTGDMKAWRHHLHAHPETAFEEHATADFIAGLLDDFGVEVHRGLAGTGVVGVIAGKRTGNRAIGLRADI
DALHVTEATGLPHASVHAGRMHACGHDGHTAMLLGAAKHLAATRDFAGRLILIFQPAEENEGGGKVMVEEGLFDRFPVDA
VYGMHNWPGLEEGHFALRTGPIMAGYDVFEITLTGKGGHAAMPHLGTDQLVAAGHLMTALQSIVARSVNPTEAAVVSVTQ
MHGGDTWNVLPASVVLRGTVRTFTKAVQDLIETRITELSRSIAQGFGAEAAIHYERRYPATVNSPEEAAVAARVASAVVG
ADKVDTNCPQTMGAEDFAFMLGVKPGAYVQLGAGPGRGGCMLHNPGYDFNDALLGVGASYWVGLVHDQLAG
>Mature_390_residues
TPAIPPAIAALTGDMKAWRHHLHAHPETAFEEHATADFIAGLLDDFGVEVHRGLAGTGVVGVIAGKRTGNRAIGLRADID
ALHVTEATGLPHASVHAGRMHACGHDGHTAMLLGAAKHLAATRDFAGRLILIFQPAEENEGGGKVMVEEGLFDRFPVDAV
YGMHNWPGLEEGHFALRTGPIMAGYDVFEITLTGKGGHAAMPHLGTDQLVAAGHLMTALQSIVARSVNPTEAAVVSVTQM
HGGDTWNVLPASVVLRGTVRTFTKAVQDLIETRITELSRSIAQGFGAEAAIHYERRYPATVNSPEEAAVAARVASAVVGA
DKVDTNCPQTMGAEDFAFMLGVKPGAYVQLGAGPGRGGCMLHNPGYDFNDALLGVGASYWVGLVHDQLAG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=424, Percent_Identity=28.0660377358491, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41039; Mature: 40908

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAIPPAIAALTGDMKAWRHHLHAHPETAFEEHATADFIAGLLDDFGVEVHRGLAGTGV
CCCCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCE
VGVIAGKRTGNRAIGLRADIDALHVTEATGLPHASVHAGRMHACGHDGHTAMLLGAAKHL
EEEEECCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEECCCCCCEEEEHHHHHHH
AATRDFAGRLILIFQPAEENEGGGKVMVEEGLFDRFPVDAVYGMHNWPGLEEGHFALRTG
HHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCEEEEEC
PIMAGYDVFEITLTGKGGHAAMPHLGTDQLVAAGHLMTALQSIVARSVNPTEAAVVSVTQ
CEEECCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEE
MHGGDTWNVLPASVVLRGTVRTFTKAVQDLIETRITELSRSIAQGFGAEAAIHYERRYPA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
TVNSPEEAAVAARVASAVVGADKVDTNCPQTMGAEDFAFMLGVKPGAYVQLGAGPGRGGC
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCE
MLHNPGYDFNDALLGVGASYWVGLVHDQLAG
EECCCCCCCCHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPAIPPAIAALTGDMKAWRHHLHAHPETAFEEHATADFIAGLLDDFGVEVHRGLAGTGV
CCCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCE
VGVIAGKRTGNRAIGLRADIDALHVTEATGLPHASVHAGRMHACGHDGHTAMLLGAAKHL
EEEEECCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEECCCCCCEEEEHHHHHHH
AATRDFAGRLILIFQPAEENEGGGKVMVEEGLFDRFPVDAVYGMHNWPGLEEGHFALRTG
HHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCEEEEEC
PIMAGYDVFEITLTGKGGHAAMPHLGTDQLVAAGHLMTALQSIVARSVNPTEAAVVSVTQ
CEEECCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEE
MHGGDTWNVLPASVVLRGTVRTFTKAVQDLIETRITELSRSIAQGFGAEAAIHYERRYPA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
TVNSPEEAAVAARVASAVVGADKVDTNCPQTMGAEDFAFMLGVKPGAYVQLGAGPGRGGC
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCE
MLHNPGYDFNDALLGVGASYWVGLVHDQLAG
EECCCCCCCCHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]