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The map label for this gene is rimO

Identifier: 83594143

GI number: 83594143

Start: 3244402

End: 3245727

Strand: Direct

Name: rimO

Synonym: Rru_A2811

Alternate gene names: 83594143

Gene position: 3244402-3245727 (Clockwise)

Preceding gene: 83594142

Following gene: 83594144

Centisome position: 74.54

GC content: 65.76

Gene sequence:

>1326_bases
ATGAACAAAGACCTTCAGAAGGTGGGCATCGTCAGCCTGGGCTGCCCCAAAGCCCTGGTCGATTCCGAACGCATCCTCAC
CCGCCTGCGGGCGGAAGGCTATGAGGTGTCGCCCACCTATGACGACGCCAATGTGGTGATCGTCAATACCTGCGGCTTCC
TCGATTCGGCCAAGGCCGAAAGCCTGGAGGCGATTGGCGAGGCCATGGCCCAGAACGGCCGGGTGATCGTGACCGGCTGC
ATGGGCGGCGACGAGGCCGCCATCCGCAGCGCCCATCCCGGCGTTCTGGCGGTCACCGGACCCCAGCAATACGAGCGCGT
GGTCGCCGCCGTCCACGAGGCGGCGCCGCCGGCCCATGACCCCTATCTTGATCTGGTGCCGGCCGAGGGCATCCGCTTGA
CGCCCCGCCATTACGCCTATCTGAAGATCTCGGAAGGCTGCAATCACGGCTGCACCTTCTGCATCATCCCCGATCTGCGC
GGCAAACTGGTCAGCCGCCCAGCCTCCAAGGTGCTGATGGAGGCCGAGAAGCTGGCCGAGGCCGGGGTGCGCGAGTTGCT
GGTGATCAGCCAGGACACCAGCGCCTATGGCGTCGATCTGCGCCACGGCGAAAGCCCCTGGCACGGCGCGCCGGTCAAGG
CCCGCATGACCGAGCTGTGTAGCGCCCTGGGCGAACTGGGGATCTGGGTGCGTCTGCACTATGTCTATCCCTATCCCTTC
GTCGACGAGATCATCCCGCTGATGGCCGAGGGCAAGATCCTGCCCTATCTGGATATCCCCTTCCAGCACGCCAGCCCGAA
GATCCTTGAGGCGATGAAGCGCCCGGCCGCCCAGGACCGCACGCTCGAGCGCATCCGCCGCTGGCGGACGATCTGCCCCG
ATATCGCGCTGCGCTCGACCTTCATCGTCGGCTTCCCCGGCGAAACCGAGGACGATTTCCAGCAGTTGCTCGACTGGATG
GGCGAAGCCGAACTCGACCGCGTCGGCTGCTTCAAATACGAGGCGGTGGCCGGGGCCAAGGCCAATGCCCTGGCCGATGC
CGTTCCCGAAGAGGTCAAGGAGGAGCGCTGGCACCGCTTCATGGCCGCCCAGCAGGAGATCAGCGAGCGCCGTCTGGCCC
AAAAGGTCGGAACGGTGATCGAGGCGATCATCGACGAAGTCGACGAGGAAGGCGCCATCGGCCGGACCAAGGGCGACGCC
CCCGAGATCGACGGCAGCCTGTTCCTCAACGGCGAAACCAGCCTCGCCCCGGGCGATATCGTGCCCGTGCTCATCGAGCA
TGCCGATGAATACGACCTGTGGGGCTCCCTTGCCGGCAAGGACTGA

Upstream 100 bases:

>100_bases
TGATCGTCTTTAACCGCGTCGGGGCGGCGAGCGGTCTGGCTTTTTGGAAGGAGTCTGGCCTATATGGGGGCTTAAAACTT
TCCCCCGCGCGCGAGAGAGC

Downstream 100 bases:

>100_bases
CATCGCCTGCGGCCTGCTGGCCGGGGGCGAGGGCAAACGCCTGGGCGGCGTCAGCAAGGCCCTGGTGCCGATCGCCGGTC
AGCCGATGGCCGCCTGGGCC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNKDLQKVGIVSLGCPKALVDSERILTRLRAEGYEVSPTYDDANVVIVNTCGFLDSAKAESLEAIGEAMAQNGRVIVTGC
MGGDEAAIRSAHPGVLAVTGPQQYERVVAAVHEAAPPAHDPYLDLVPAEGIRLTPRHYAYLKISEGCNHGCTFCIIPDLR
GKLVSRPASKVLMEAEKLAEAGVRELLVISQDTSAYGVDLRHGESPWHGAPVKARMTELCSALGELGIWVRLHYVYPYPF
VDEIIPLMAEGKILPYLDIPFQHASPKILEAMKRPAAQDRTLERIRRWRTICPDIALRSTFIVGFPGETEDDFQQLLDWM
GEAELDRVGCFKYEAVAGAKANALADAVPEEVKEERWHRFMAAQQEISERRLAQKVGTVIEAIIDEVDEEGAIGRTKGDA
PEIDGSLFLNGETSLAPGDIVPVLIEHADEYDLWGSLAGKD

Sequences:

>Translated_441_residues
MNKDLQKVGIVSLGCPKALVDSERILTRLRAEGYEVSPTYDDANVVIVNTCGFLDSAKAESLEAIGEAMAQNGRVIVTGC
MGGDEAAIRSAHPGVLAVTGPQQYERVVAAVHEAAPPAHDPYLDLVPAEGIRLTPRHYAYLKISEGCNHGCTFCIIPDLR
GKLVSRPASKVLMEAEKLAEAGVRELLVISQDTSAYGVDLRHGESPWHGAPVKARMTELCSALGELGIWVRLHYVYPYPF
VDEIIPLMAEGKILPYLDIPFQHASPKILEAMKRPAAQDRTLERIRRWRTICPDIALRSTFIVGFPGETEDDFQQLLDWM
GEAELDRVGCFKYEAVAGAKANALADAVPEEVKEERWHRFMAAQQEISERRLAQKVGTVIEAIIDEVDEEGAIGRTKGDA
PEIDGSLFLNGETSLAPGDIVPVLIEHADEYDLWGSLAGKD
>Mature_441_residues
MNKDLQKVGIVSLGCPKALVDSERILTRLRAEGYEVSPTYDDANVVIVNTCGFLDSAKAESLEAIGEAMAQNGRVIVTGC
MGGDEAAIRSAHPGVLAVTGPQQYERVVAAVHEAAPPAHDPYLDLVPAEGIRLTPRHYAYLKISEGCNHGCTFCIIPDLR
GKLVSRPASKVLMEAEKLAEAGVRELLVISQDTSAYGVDLRHGESPWHGAPVKARMTELCSALGELGIWVRLHYVYPYPF
VDEIIPLMAEGKILPYLDIPFQHASPKILEAMKRPAAQDRTLERIRRWRTICPDIALRSTFIVGFPGETEDDFQQLLDWM
GEAELDRVGCFKYEAVAGAKANALADAVPEEVKEERWHRFMAAQQEISERRLAQKVGTVIEAIIDEVDEEGAIGRTKGDA
PEIDGSLFLNGETSLAPGDIVPVLIEHADEYDLWGSLAGKD

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=25.9958071278826, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=25.9958071278826, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI93277076, Length=389, Percent_Identity=26.4781491002571, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787057, Length=431, Percent_Identity=69.3735498839907, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=456, Percent_Identity=27.4122807017544, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=484, Percent_Identity=25.2066115702479, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI71996771, Length=269, Percent_Identity=26.7657992565056, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356207, Length=487, Percent_Identity=23.8193018480493, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI19922432, Length=412, Percent_Identity=25, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_RHORT (Q2RQI7)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_427895.1
- ProteinModelPortal:   Q2RQI7
- SMR:   Q2RQI7
- STRING:   Q2RQI7
- GeneID:   3836251
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A2811
- NMPDR:   fig|1085.1.peg.456
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q2RQI7
- ProtClustDB:   PRK14862
- BioCyc:   RRUB269796:RRU_A2811-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48307; Mature: 48307

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDLQKVGIVSLGCPKALVDSERILTRLRAEGYEVSPTYDDANVVIVNTCGFLDSAKAE
CCCCHHHHCEEECCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCCCCHHHH
SLEAIGEAMAQNGRVIVTGCMGGDEAAIRSAHPGVLAVTGPQQYERVVAAVHEAAPPAHD
HHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCC
PYLDLVPAEGIRLTPRHYAYLKISEGCNHGCTFCIIPDLRGKLVSRPASKVLMEAEKLAE
CCEEEECCCCEEECCCEEEEEEECCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHH
AGVRELLVISQDTSAYGVDLRHGESPWHGAPVKARMTELCSALGELGIWVRLHYVYPYPF
CCHHEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCC
VDEIIPLMAEGKILPYLDIPFQHASPKILEAMKRPAAQDRTLERIRRWRTICPDIALRST
HHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHCE
FIVGFPGETEDDFQQLLDWMGEAELDRVGCFKYEAVAGAKANALADAVPEEVKEERWHRF
EEEECCCCCHHHHHHHHHHCCCCCHHHHCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHH
MAAQQEISERRLAQKVGTVIEAIIDEVDEEGAIGRTKGDAPEIDGSLFLNGETSLAPGDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCH
VPVLIEHADEYDLWGSLAGKD
HHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure
MNKDLQKVGIVSLGCPKALVDSERILTRLRAEGYEVSPTYDDANVVIVNTCGFLDSAKAE
CCCCHHHHCEEECCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCCCCHHHH
SLEAIGEAMAQNGRVIVTGCMGGDEAAIRSAHPGVLAVTGPQQYERVVAAVHEAAPPAHD
HHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCC
PYLDLVPAEGIRLTPRHYAYLKISEGCNHGCTFCIIPDLRGKLVSRPASKVLMEAEKLAE
CCEEEECCCCEEECCCEEEEEEECCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHH
AGVRELLVISQDTSAYGVDLRHGESPWHGAPVKARMTELCSALGELGIWVRLHYVYPYPF
CCHHEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCC
VDEIIPLMAEGKILPYLDIPFQHASPKILEAMKRPAAQDRTLERIRRWRTICPDIALRST
HHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHCE
FIVGFPGETEDDFQQLLDWMGEAELDRVGCFKYEAVAGAKANALADAVPEEVKEERWHRF
EEEECCCCCHHHHHHHHHHCCCCCHHHHCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHH
MAAQQEISERRLAQKVGTVIEAIIDEVDEEGAIGRTKGDAPEIDGSLFLNGETSLAPGDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCH
VPVLIEHADEYDLWGSLAGKD
HHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA