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The map label for this gene is nikA [H]

Identifier: 83593612

GI number: 83593612

Start: 2635578

End: 2637239

Strand: Reverse

Name: nikA [H]

Synonym: Rru_A2277

Alternate gene names: 83593612

Gene position: 2637239-2635578 (Counterclockwise)

Preceding gene: 83593614

Following gene: 83593611

Centisome position: 60.59

GC content: 63.54

Gene sequence:

>1662_bases
ATGCCGGCCGTCGGCTTGTGTTCGGAGGTCCGGGCAGGTTGGGACGGGGGGGCCGTCGTCAGGGGAAGGGGAAAGACCGT
GAGATCGTTTCATGCTTTGGTGGGGGCGCTTGGCATCGCGTTATGCGCGCTGTCGCCCTTTGGCGCCGCCCGCGCCGGCG
AAACCTTGAATTTTTCCTGGCCGCAGAACGTCGGCCCGCTCAATCCCCATCTCTATTCTCCCAATCAGATGTTCGCCCAG
GCGATGGTCTATGAGCCGCTGGTGCGCTATCGGGCCGATGGCGCCGTGGTGCCCTGGTTGGCGCGCTCGTGGGAGATTTC
GGCCGATGGCCGGGTCTATACCTTCTCCCTGCGTCCCGATGTGCTCTTTGCCAATGGCGAGGCCTTTGACGCCAAGGCGG
TGGTCGCCAATTTCGACGCGGTTCTGGCCAACCGCGCCCGCCACCAATGGCTGGAACTGGCCAATCAGATCGAGAGGGTC
GAGGTCGTCGACGACCTGGCGTTTCGCCTGACCTTGAAAGAGGCCTATTACCCGATCCTTCAGGAACTCGCCCTGCCGCG
GCCTTTCCGCTTCATCGCGCCTTCGCGCTTTGGCAAGGCCGGCGGCACCAAGGACGGCATTGTGGAGCCGGTCGGCACCG
GACCTTGGAAATTGGTGGCCAGCGGCTTGGGCGAATACGATCTGTTCGAGCGCAACGACGCCTATTGGGGCGAGAAGCCG
GCCTATGACAAGATCATGGTCAAGGTCATCCCCGATCCGACGACCCGGGCCATCGCCTTTGAAACCGGCGATATCGATCT
GATCTACGGTGTGGATGGTCCGGTCTATCCCGATACCTTCGACCGCCTGCGCGCCATGGGCCTCTATACGGCCGCCCTGT
CGGCTCCGGTGGAAACCCGGGTGCTGGCGCTCAATTCCAACCGCGCGCCGACCAATGAGCTGGCGGTGCGGCAGGCGCTC
AATCACGCCATCGACAAGGACACGATGATCGCCTCGGTCTTCTATGGCACCCAGACCCGCGCCGACACCTTGTTTTCGAC
CAATGTGCCCTATGCCGATATCGCTCTGAAGCCCTATGCCTATGATCGGGCGCGGGCGGCCGCCTTGCTCGAGGCGGCGA
ACTGGCGTTTCGAGGCGGGAAGCGACCTGCGCAGCAAGGACGGCAAGCCGCTGCTCATCGATCTCGTCTTCGTCGGCACC
GACGCGGTCGCCAAATCGATGGCGGAAATCGTCCAGGGGGATCTGCGCAAGATCGGTGTCGGGGCCGTTCTTGTCGGCGA
GGAGGAAAGCAGCGTCTTTTCGCGCCAGCATGATGGTCGCTTCGGCATGATCTTCAATTCCACCTGGGGCGCCCCCTTCG
ATCCCCATGCCTTCGTCAGTTCGATGCGCGTGCCCTCCCATGCCGATTATCAGGCCCAACGTGGCCTGCCCGACAAGGCC
GAGATCGACGCGACGATCTCGCGGGTTCTGGTCACCACCGACGAGACGGAGCGTCAGGCGCTGTACACCAAGATCCTGAC
CCGCCTGCATGATCAGGCGGTCTATCTGCCGCTGACCCATGTCACGGCGATCGCCCTGGCCAAGAAGGCGGTGGGGCCCT
TGTCGTTCGGGGCGATGTCGAGCGAGATCCCCTTCGAAAACCTCGTTCCCCTGGCGCCGTGA

Upstream 100 bases:

>100_bases
AACGCTTTGGCGCCTGTTGGTTGCGGCCTTGAAACACGCAAAAAACGCTCACGAATTCCCACACTCCCGACATTGACATT
ATGCGACTGGGTCATCCAGT

Downstream 100 bases:

>100_bases
TCGTTATGGTCTCTTTCGTTCTCAAGCGCTTGGCGCTGCTCGTTCCCATGTTGCTCGGCGCCTCGCTGGTCATCTTCCTG
ATGCTGCGCCTGGGGCCCAG

Product: nickel ABC transporter periplasmic nickel-binding

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MPAVGLCSEVRAGWDGGAVVRGRGKTVRSFHALVGALGIALCALSPFGAARAGETLNFSWPQNVGPLNPHLYSPNQMFAQ
AMVYEPLVRYRADGAVVPWLARSWEISADGRVYTFSLRPDVLFANGEAFDAKAVVANFDAVLANRARHQWLELANQIERV
EVVDDLAFRLTLKEAYYPILQELALPRPFRFIAPSRFGKAGGTKDGIVEPVGTGPWKLVASGLGEYDLFERNDAYWGEKP
AYDKIMVKVIPDPTTRAIAFETGDIDLIYGVDGPVYPDTFDRLRAMGLYTAALSAPVETRVLALNSNRAPTNELAVRQAL
NHAIDKDTMIASVFYGTQTRADTLFSTNVPYADIALKPYAYDRARAAALLEAANWRFEAGSDLRSKDGKPLLIDLVFVGT
DAVAKSMAEIVQGDLRKIGVGAVLVGEEESSVFSRQHDGRFGMIFNSTWGAPFDPHAFVSSMRVPSHADYQAQRGLPDKA
EIDATISRVLVTTDETERQALYTKILTRLHDQAVYLPLTHVTAIALAKKAVGPLSFGAMSSEIPFENLVPLAP

Sequences:

>Translated_553_residues
MPAVGLCSEVRAGWDGGAVVRGRGKTVRSFHALVGALGIALCALSPFGAARAGETLNFSWPQNVGPLNPHLYSPNQMFAQ
AMVYEPLVRYRADGAVVPWLARSWEISADGRVYTFSLRPDVLFANGEAFDAKAVVANFDAVLANRARHQWLELANQIERV
EVVDDLAFRLTLKEAYYPILQELALPRPFRFIAPSRFGKAGGTKDGIVEPVGTGPWKLVASGLGEYDLFERNDAYWGEKP
AYDKIMVKVIPDPTTRAIAFETGDIDLIYGVDGPVYPDTFDRLRAMGLYTAALSAPVETRVLALNSNRAPTNELAVRQAL
NHAIDKDTMIASVFYGTQTRADTLFSTNVPYADIALKPYAYDRARAAALLEAANWRFEAGSDLRSKDGKPLLIDLVFVGT
DAVAKSMAEIVQGDLRKIGVGAVLVGEEESSVFSRQHDGRFGMIFNSTWGAPFDPHAFVSSMRVPSHADYQAQRGLPDKA
EIDATISRVLVTTDETERQALYTKILTRLHDQAVYLPLTHVTAIALAKKAVGPLSFGAMSSEIPFENLVPLAP
>Mature_552_residues
PAVGLCSEVRAGWDGGAVVRGRGKTVRSFHALVGALGIALCALSPFGAARAGETLNFSWPQNVGPLNPHLYSPNQMFAQA
MVYEPLVRYRADGAVVPWLARSWEISADGRVYTFSLRPDVLFANGEAFDAKAVVANFDAVLANRARHQWLELANQIERVE
VVDDLAFRLTLKEAYYPILQELALPRPFRFIAPSRFGKAGGTKDGIVEPVGTGPWKLVASGLGEYDLFERNDAYWGEKPA
YDKIMVKVIPDPTTRAIAFETGDIDLIYGVDGPVYPDTFDRLRAMGLYTAALSAPVETRVLALNSNRAPTNELAVRQALN
HAIDKDTMIASVFYGTQTRADTLFSTNVPYADIALKPYAYDRARAAALLEAANWRFEAGSDLRSKDGKPLLIDLVFVGTD
AVAKSMAEIVQGDLRKIGVGAVLVGEEESSVFSRQHDGRFGMIFNSTWGAPFDPHAFVSSMRVPSHADYQAQRGLPDKAE
IDATISRVLVTTDETERQALYTKILTRLHDQAVYLPLTHVTAIALAKKAVGPLSFGAMSSEIPFENLVPLAP

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=516, Percent_Identity=59.8837209302326, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=525, Percent_Identity=26.0952380952381, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1787052, Length=499, Percent_Identity=24.4488977955912, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787762, Length=469, Percent_Identity=23.2409381663113, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1787495, Length=528, Percent_Identity=24.8106060606061, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787551, Length=273, Percent_Identity=25.2747252747253, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1789397, Length=489, Percent_Identity=22.6993865030675, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60394; Mature: 60263

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVGLCSEVRAGWDGGAVVRGRGKTVRSFHALVGALGIALCALSPFGAARAGETLNFSW
CCCCCCHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
PQNVGPLNPHLYSPNQMFAQAMVYEPLVRYRADGAVVPWLARSWEISADGRVYTFSLRPD
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEECCCCEEEEEEECCC
VLFANGEAFDAKAVVANFDAVLANRARHQWLELANQIERVEVVDDLAFRLTLKEAYYPIL
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHH
QELALPRPFRFIAPSRFGKAGGTKDGIVEPVGTGPWKLVASGLGEYDLFERNDAYWGEKP
HHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
AYDKIMVKVIPDPTTRAIAFETGDIDLIYGVDGPVYPDTFDRLRAMGLYTAALSAPVETR
CCCEEEEEECCCCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
VLALNSNRAPTNELAVRQALNHAIDKDTMIASVFYGTQTRADTLFSTNVPYADIALKPYA
EEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEECCCH
YDRARAAALLEAANWRFEAGSDLRSKDGKPLLIDLVFVGTDAVAKSMAEIVQGDLRKIGV
HHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCC
GAVLVGEEESSVFSRQHDGRFGMIFNSTWGAPFDPHAFVSSMRVPSHADYQAQRGLPDKA
CEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHCCCCCHH
EIDATISRVLVTTDETERQALYTKILTRLHDQAVYLPLTHVTAIALAKKAVGPLSFGAMS
HHHHEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCC
SEIPFENLVPLAP
CCCCHHHCCCCCC
>Mature Secondary Structure 
PAVGLCSEVRAGWDGGAVVRGRGKTVRSFHALVGALGIALCALSPFGAARAGETLNFSW
CCCCCHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
PQNVGPLNPHLYSPNQMFAQAMVYEPLVRYRADGAVVPWLARSWEISADGRVYTFSLRPD
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEECCCCEEEEEEECCC
VLFANGEAFDAKAVVANFDAVLANRARHQWLELANQIERVEVVDDLAFRLTLKEAYYPIL
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHH
QELALPRPFRFIAPSRFGKAGGTKDGIVEPVGTGPWKLVASGLGEYDLFERNDAYWGEKP
HHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
AYDKIMVKVIPDPTTRAIAFETGDIDLIYGVDGPVYPDTFDRLRAMGLYTAALSAPVETR
CCCEEEEEECCCCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
VLALNSNRAPTNELAVRQALNHAIDKDTMIASVFYGTQTRADTLFSTNVPYADIALKPYA
EEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEECCCH
YDRARAAALLEAANWRFEAGSDLRSKDGKPLLIDLVFVGTDAVAKSMAEIVQGDLRKIGV
HHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCC
GAVLVGEEESSVFSRQHDGRFGMIFNSTWGAPFDPHAFVSSMRVPSHADYQAQRGLPDKA
CEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHCCCCCHH
EIDATISRVLVTTDETERQALYTKILTRLHDQAVYLPLTHVTAIALAKKAVGPLSFGAMS
HHHHEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCC
SEIPFENLVPLAP
CCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]