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The map label for this gene is nikB [H]

Identifier: 83593611

GI number: 83593611

Start: 2634628

End: 2635572

Strand: Reverse

Name: nikB [H]

Synonym: Rru_A2276

Alternate gene names: 83593611

Gene position: 2635572-2634628 (Counterclockwise)

Preceding gene: 83593612

Following gene: 83593610

Centisome position: 60.55

GC content: 64.87

Gene sequence:

>945_bases
ATGGTCTCTTTCGTTCTCAAGCGCTTGGCGCTGCTCGTTCCCATGTTGCTCGGCGCCTCGCTGGTCATCTTCCTGATGCT
GCGCCTGGGGCCCAGCGATCCGGCGATGGATTACCTGCGGCTATCGCGCATTCCGCCGACCGTCCAGGCCCTGGCCGAGA
CCCGCCAGAGGCTGGGGCTCGATCAGCCGCTGGCGGTTCAGTATTGGGTTTGGCTGAACAAGGCCCTGCATCTGGATTTC
GGCGTGTCCTACGCCACCGGGCGGCCGGTTCTGCCCGATCTTTTGCATTATCTGCCCGCCACCTTGCACTTGGCCGGGGT
GGCCCTGGCGCTGACCCTGGGCCTGTCGATCCCTTTGGGGATGTGGGCGGCGCGGCGGCGCGACGCCTTACCCGACCAGA
TCGTTCGCGGCGTCGCCTTTCTCGGCGTCTCGATGCCCAATTTCTGGCTGGGCTTCCTGCTGGTGCTGGTGTTTTCGGTC
GAGCTTGGCTGGCTGCCGGCGATGGGGCGGGGCGGGGCGGAGCATATGATCATGCCCGCCGTCGCCATCGCCTTCATGTC
GCTATCGATCAACGCCCGGCTGCTGCGCGCCAGCATGCTCGAGGTGTCCGGGCAGCGTCACGTCTATTACGCCCGCCTGC
GCGGCCTGTCGGCGGCCAAGGTGGAACGCGCCCATATCCTGCGCAACGCCCTGCTGCCGATCATCACGGCGACGGGCATG
CACGTTGGCGAACTGATCGGCGGCACCCTGGTCATTGAAAGCATTTTCGGCTGGCCGGGGGTTGGCCGCTTTGCGGTCTC
CGCCATCTTCAACCGCGATTATCCGGTCATCCAGTGTTTCACGCTGCTGATGGTGCTGATCTTCGTTCTTTGCAATCTGA
TCGTCGATATCGTCTATGCCTGGGCCGATCCGCGCATCCGGCTGGCGGCGGAGGGGGCCCAATGA

Upstream 100 bases:

>100_bases
CGATCGCCCTGGCCAAGAAGGCGGTGGGGCCCTTGTCGTTCGGGGCGATGTCGAGCGAGATCCCCTTCGAAAACCTCGTT
CCCCTGGCGCCGTGATCGTT

Downstream 100 bases:

>100_bases
CCATGCCTGATTGCCAGCAGGGGCATCGCCCGCCAGACGCCGCCCCGCTCCGGGGGTGGCTCGCCCGCCTGCCCATCGGC
CGAAGCTGGAGCCTGCGCTT

Product: nickel transporter permease NikB

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MVSFVLKRLALLVPMLLGASLVIFLMLRLGPSDPAMDYLRLSRIPPTVQALAETRQRLGLDQPLAVQYWVWLNKALHLDF
GVSYATGRPVLPDLLHYLPATLHLAGVALALTLGLSIPLGMWAARRRDALPDQIVRGVAFLGVSMPNFWLGFLLVLVFSV
ELGWLPAMGRGGAEHMIMPAVAIAFMSLSINARLLRASMLEVSGQRHVYYARLRGLSAAKVERAHILRNALLPIITATGM
HVGELIGGTLVIESIFGWPGVGRFAVSAIFNRDYPVIQCFTLLMVLIFVLCNLIVDIVYAWADPRIRLAAEGAQ

Sequences:

>Translated_314_residues
MVSFVLKRLALLVPMLLGASLVIFLMLRLGPSDPAMDYLRLSRIPPTVQALAETRQRLGLDQPLAVQYWVWLNKALHLDF
GVSYATGRPVLPDLLHYLPATLHLAGVALALTLGLSIPLGMWAARRRDALPDQIVRGVAFLGVSMPNFWLGFLLVLVFSV
ELGWLPAMGRGGAEHMIMPAVAIAFMSLSINARLLRASMLEVSGQRHVYYARLRGLSAAKVERAHILRNALLPIITATGM
HVGELIGGTLVIESIFGWPGVGRFAVSAIFNRDYPVIQCFTLLMVLIFVLCNLIVDIVYAWADPRIRLAAEGAQ
>Mature_314_residues
MVSFVLKRLALLVPMLLGASLVIFLMLRLGPSDPAMDYLRLSRIPPTVQALAETRQRLGLDQPLAVQYWVWLNKALHLDF
GVSYATGRPVLPDLLHYLPATLHLAGVALALTLGLSIPLGMWAARRRDALPDQIVRGVAFLGVSMPNFWLGFLLVLVFSV
ELGWLPAMGRGGAEHMIMPAVAIAFMSLSINARLLRASMLEVSGQRHVYYARLRGLSAAKVERAHILRNALLPIITATGM
HVGELIGGTLVIESIFGWPGVGRFAVSAIFNRDYPVIQCFTLLMVLIFVLCNLIVDIVYAWADPRIRLAAEGAQ

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=314, Percent_Identity=70.7006369426752, Blast_Score=446, Evalue=1e-127,
Organism=Escherichia coli, GI1787053, Length=308, Percent_Identity=39.9350649350649, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789965, Length=340, Percent_Identity=35, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1787497, Length=310, Percent_Identity=34.8387096774194, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1787761, Length=334, Percent_Identity=37.125748502994, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787550, Length=268, Percent_Identity=33.5820895522388, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1788504, Length=281, Percent_Identity=26.3345195729537, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34481; Mature: 34481

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSFVLKRLALLVPMLLGASLVIFLMLRLGPSDPAMDYLRLSRIPPTVQALAETRQRLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC
DQPLAVQYWVWLNKALHLDFGVSYATGRPVLPDLLHYLPATLHLAGVALALTLGLSIPLG
CCCHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
MWAARRRDALPDQIVRGVAFLGVSMPNFWLGFLLVLVFSVELGWLPAMGRGGAEHMIMPA
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VAIAFMSLSINARLLRASMLEVSGQRHVYYARLRGLSAAKVERAHILRNALLPIITATGM
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
HVGELIGGTLVIESIFGWPGVGRFAVSAIFNRDYPVIQCFTLLMVLIFVLCNLIVDIVYA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WADPRIRLAAEGAQ
CCCCCEEEECCCCC
>Mature Secondary Structure
MVSFVLKRLALLVPMLLGASLVIFLMLRLGPSDPAMDYLRLSRIPPTVQALAETRQRLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC
DQPLAVQYWVWLNKALHLDFGVSYATGRPVLPDLLHYLPATLHLAGVALALTLGLSIPLG
CCCHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
MWAARRRDALPDQIVRGVAFLGVSMPNFWLGFLLVLVFSVELGWLPAMGRGGAEHMIMPA
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VAIAFMSLSINARLLRASMLEVSGQRHVYYARLRGLSAAKVERAHILRNALLPIITATGM
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
HVGELIGGTLVIESIFGWPGVGRFAVSAIFNRDYPVIQCFTLLMVLIFVLCNLIVDIVYA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WADPRIRLAAEGAQ
CCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]