Click here to switch to the map view.

The map label for this gene is fumA [H]

Identifier: 83593541

GI number: 83593541

Start: 2561938

End: 2563584

Strand: Direct

Name: fumA [H]

Synonym: Rru_A2206

Alternate gene names: 83593541

Gene position: 2561938-2563584 (Clockwise)

Preceding gene: 83593540

Following gene: 83593542

Centisome position: 58.86

GC content: 65.03

Gene sequence:

>1647_bases
ATGACCGATGTATCGGGGGATACCAGGATCATGATCGATCGCGCTTTCGCGGACGTCTTTCCCTTGGCGGAGGATAAAAC
CCTCTACCGCGCTCTCGAAGGGACGGAGGGGTTGGTTTCGGTGGAGCGGTTCCGCGGCGAGGAGATGCTGGTCGTCGCCC
CCGAGGCCCTGACCCGTCTGGCGCAAGAGGCCTTCCGCGACATCGCCCATCTGCTGCGCCCGGCCCATCTTTCCCAGCTG
CGCGCCATCATGGACGATCCCGAGGCTTCGGCCAACGACCGCTTCGTCGCCCTGGAACTGCTGAAAAACGCCAATATCTC
GGCCGGCATGGTCTTGCCGATGTGCCAGGACACCGGCACGGCGATCATTCAGGGCAAGAAGGGCCAGCGGGTGTTTTCGG
GCGACGACGACGCCCTCGCCCTGTCGGAAGGCGTGGCCCGGACCTATCAGACCCTGAACCTGCGCTATTCGCAGATGGCG
GCGCTGTCGGTTTTCGACGAGGTCAATACCGGCAACAACCTGCCCGCCCAGATCGACCTCTACGCCACGCCGGGCGACGC
CTATAAATTCCTCTTCATGGCCAAAGGCGGCGGCTCGGCCAATAAGGTCTTCCTTTACCAGCAGACCAAGGCCCTGCTCA
ATCCCGAGAGCCTCGCCCGCTTCCTTGACGCCCAGGTGCGCACGCTGGGCACCGCCGCCTGCCCGCCCTATCACCTGTCG
ATCGCCATCGGCGGCACCAGCGCCGAGGCCTGCCTGAAGGCGGTGAAACTGGCCAGCGCCAAGTATCTCGATGGCCTGCC
GACCACCGGGGGCAAGGATGGCCACGCCTTCCGCGATCTGGCGCTCGAGGCCGAGGTTCTGGCGATGACCCGGGCCATGG
GCATCGGCGCCCAGTTCGGCGGCAAGTATTTCTGCCACGACGTGCGCGTTGTCCGCCTGCCCCGCCACGGCGCGAGCTGC
CCGGTGGGCATCGGCGTCTCCTGCTCGGCCGACCGCCAGATGCTGGGCAAGATCACCGCCGAAGGGCTGTTCCTCGAACA
GCTTGAAACCAATCCCGCCCATTACCTGCCCGACGTGCGCACCGAGAAGCTGTCGGCCGAGGTGGTCAAGATCGACCTGT
CGCGGCCGATGGCGGAAATCCGCGCGACGCTCAGCGGCTATCCGATCAAGACCCGGGTCTCGCTGTCGGGGCCGATGATC
GTCGCCCGCGACATCGCCCACGCCAAGCTCAAGGAACGGCTGGATCGCGGCGAGGGCCTGCCCGAGTACTTCAAGAACGG
CGCGGTCTATTACGCCGGCCCGGCCAAGACCCCGGCCAATTACGCCTCGGGCTCCTTTGGCCCGACCACCGCCGGCCGCA
TGGACGCCTATGTCGATCTATTCCAAGAGAACGGCGGCTCGATGGTGATGATCGCCAAGGGCAACCGTTCCAAGGCGGTG
ACCGAGGCCTGCAAGAAGCACGGCGGGTTCTATCTGGGCTCGATCGGCGGCGCCGCCGCCCAACTGGCCCTCAACAGCAT
CCGCAAGGTCGAGGTTCTGGAATACCCGGAACTGGGCATGGAAGCCATCTGGCGCATCGAGGTCGAGGATTTTCCGGCCT
TCATCATCGTCGATGACAAGGGCAACGACTTCTTCCAGCAGCTTTGA

Upstream 100 bases:

>100_bases
GAATAAGGGGTGGGGCATGGCGAACGTCCCGCCCCACCCACCAAGGCCCATCCAAGGCCCATGACCATAAGCCCCCCTCG
CCCGGGGGACGGCTGTTGAA

Downstream 100 bases:

>100_bases
GACTGCGATTGCGCGTCTCCTCCCCCGGTTCGAGAGGGAGGAGGCGGTGGAGCGAAGCGGGAGCCGGACCAAGCATGGAT
GACAAAGAACGCGGCCGCTA

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MTDVSGDTRIMIDRAFADVFPLAEDKTLYRALEGTEGLVSVERFRGEEMLVVAPEALTRLAQEAFRDIAHLLRPAHLSQL
RAIMDDPEASANDRFVALELLKNANISAGMVLPMCQDTGTAIIQGKKGQRVFSGDDDALALSEGVARTYQTLNLRYSQMA
ALSVFDEVNTGNNLPAQIDLYATPGDAYKFLFMAKGGGSANKVFLYQQTKALLNPESLARFLDAQVRTLGTAACPPYHLS
IAIGGTSAEACLKAVKLASAKYLDGLPTTGGKDGHAFRDLALEAEVLAMTRAMGIGAQFGGKYFCHDVRVVRLPRHGASC
PVGIGVSCSADRQMLGKITAEGLFLEQLETNPAHYLPDVRTEKLSAEVVKIDLSRPMAEIRATLSGYPIKTRVSLSGPMI
VARDIAHAKLKERLDRGEGLPEYFKNGAVYYAGPAKTPANYASGSFGPTTAGRMDAYVDLFQENGGSMVMIAKGNRSKAV
TEACKKHGGFYLGSIGGAAAQLALNSIRKVEVLEYPELGMEAIWRIEVEDFPAFIIVDDKGNDFFQQL

Sequences:

>Translated_548_residues
MTDVSGDTRIMIDRAFADVFPLAEDKTLYRALEGTEGLVSVERFRGEEMLVVAPEALTRLAQEAFRDIAHLLRPAHLSQL
RAIMDDPEASANDRFVALELLKNANISAGMVLPMCQDTGTAIIQGKKGQRVFSGDDDALALSEGVARTYQTLNLRYSQMA
ALSVFDEVNTGNNLPAQIDLYATPGDAYKFLFMAKGGGSANKVFLYQQTKALLNPESLARFLDAQVRTLGTAACPPYHLS
IAIGGTSAEACLKAVKLASAKYLDGLPTTGGKDGHAFRDLALEAEVLAMTRAMGIGAQFGGKYFCHDVRVVRLPRHGASC
PVGIGVSCSADRQMLGKITAEGLFLEQLETNPAHYLPDVRTEKLSAEVVKIDLSRPMAEIRATLSGYPIKTRVSLSGPMI
VARDIAHAKLKERLDRGEGLPEYFKNGAVYYAGPAKTPANYASGSFGPTTAGRMDAYVDLFQENGGSMVMIAKGNRSKAV
TEACKKHGGFYLGSIGGAAAQLALNSIRKVEVLEYPELGMEAIWRIEVEDFPAFIIVDDKGNDFFQQL
>Mature_547_residues
TDVSGDTRIMIDRAFADVFPLAEDKTLYRALEGTEGLVSVERFRGEEMLVVAPEALTRLAQEAFRDIAHLLRPAHLSQLR
AIMDDPEASANDRFVALELLKNANISAGMVLPMCQDTGTAIIQGKKGQRVFSGDDDALALSEGVARTYQTLNLRYSQMAA
LSVFDEVNTGNNLPAQIDLYATPGDAYKFLFMAKGGGSANKVFLYQQTKALLNPESLARFLDAQVRTLGTAACPPYHLSI
AIGGTSAEACLKAVKLASAKYLDGLPTTGGKDGHAFRDLALEAEVLAMTRAMGIGAQFGGKYFCHDVRVVRLPRHGASCP
VGIGVSCSADRQMLGKITAEGLFLEQLETNPAHYLPDVRTEKLSAEVVKIDLSRPMAEIRATLSGYPIKTRVSLSGPMIV
ARDIAHAKLKERLDRGEGLPEYFKNGAVYYAGPAKTPANYASGSFGPTTAGRMDAYVDLFQENGGSMVMIAKGNRSKAVT
EACKKHGGFYLGSIGGAAAQLALNSIRKVEVLEYPELGMEAIWRIEVEDFPAFIIVDDKGNDFFQQL

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=529, Percent_Identity=65.2173913043478, Blast_Score=718, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=529, Percent_Identity=65.0283553875236, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=159, Percent_Identity=29.559748427673, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 59225; Mature: 59093

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVSGDTRIMIDRAFADVFPLAEDKTLYRALEGTEGLVSVERFRGEEMLVVAPEALTRL
CCCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHCCCCCCEEHHHHCCCEEEEECHHHHHHH
AQEAFRDIAHLLRPAHLSQLRAIMDDPEASANDRFVALELLKNANISAGMVLPMCQDTGT
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEEHHCCCCCCCEEEEEECCCCC
AIIQGKKGQRVFSGDDDALALSEGVARTYQTLNLRYSQMAALSVFDEVNTGNNLPAQIDL
EEEECCCCCEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEE
YATPGDAYKFLFMAKGGGSANKVFLYQQTKALLNPESLARFLDAQVRTLGTAACPPYHLS
EECCCCCEEEEEEECCCCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEE
IAIGGTSAEACLKAVKLASAKYLDGLPTTGGKDGHAFRDLALEAEVLAMTRAMGIGAQFG
EEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCC
GKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQMLGKITAEGLFLEQLETNPAHYLPDVR
CEEEEECEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEHHCCCCHHCCCCCC
TEKLSAEVVKIDLSRPMAEIRATLSGYPIKTRVSLSGPMIVARDIAHAKLKERLDRGEGL
HHHCCEEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCC
PEYFKNGAVYYAGPAKTPANYASGSFGPTTAGRMDAYVDLFQENGGSMVMIAKGNRSKAV
HHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHH
TEACKKHGGFYLGSIGGAAAQLALNSIRKVEVLEYPELGMEAIWRIEVEDFPAFIIVDDK
HHHHHHHCCEEEECCCHHHHHHHHHHHCEEEEECCCCCCCEEEEEEEECCCCEEEEEECC
GNDFFQQL
CCHHHHCC
>Mature Secondary Structure 
TDVSGDTRIMIDRAFADVFPLAEDKTLYRALEGTEGLVSVERFRGEEMLVVAPEALTRL
CCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHCCCCCCEEHHHHCCCEEEEECHHHHHHH
AQEAFRDIAHLLRPAHLSQLRAIMDDPEASANDRFVALELLKNANISAGMVLPMCQDTGT
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEEHHCCCCCCCEEEEEECCCCC
AIIQGKKGQRVFSGDDDALALSEGVARTYQTLNLRYSQMAALSVFDEVNTGNNLPAQIDL
EEEECCCCCEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEE
YATPGDAYKFLFMAKGGGSANKVFLYQQTKALLNPESLARFLDAQVRTLGTAACPPYHLS
EECCCCCEEEEEEECCCCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEE
IAIGGTSAEACLKAVKLASAKYLDGLPTTGGKDGHAFRDLALEAEVLAMTRAMGIGAQFG
EEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCC
GKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQMLGKITAEGLFLEQLETNPAHYLPDVR
CEEEEECEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEHHCCCCHHCCCCCC
TEKLSAEVVKIDLSRPMAEIRATLSGYPIKTRVSLSGPMIVARDIAHAKLKERLDRGEGL
HHHCCEEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCC
PEYFKNGAVYYAGPAKTPANYASGSFGPTTAGRMDAYVDLFQENGGSMVMIAKGNRSKAV
HHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHH
TEACKKHGGFYLGSIGGAAAQLALNSIRKVEVLEYPELGMEAIWRIEVEDFPAFIIVDDK
HHHHHHHCCEEEECCCHHHHHHHHHHHCEEEEECCCCCCCEEEEEEEECCCCEEEEEECC
GNDFFQQL
CCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]