BacMap

Definition	Rhodospirillum rubrum ATCC 11170 chromosome, complete genome.
Accession	NC_007643
Length	4,352,825

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The map label for this gene is paaF [C]

Identifier: 83593491

GI number: 83593491

Start: 2504014

End: 2504799

Strand: Direct

Name: paaF [C]

Synonym: Rru_A2156

Alternate gene names: 83593491

Gene position: 2504014-2504799 (Clockwise)

Preceding gene: 83593485

Following gene: 83593492

Centisome position: 57.53

GC content: 69.21

Gene sequence:

>786_bases
ATGACGGTTCCCCCTCCCCTGCTGGAGTCCCTTACCGTTGACGGCGTGCGCACCCTGACCCTCAACCGCCCGCAAGCCCG
CAACGCGCTGTCAAAGGCCTTGATGGCCGCCCTGCAAGACGCCCTGGATCAGGCGGCGGAGGATCCGGCGGTGCGGGTGG
TGGTGATCGCCGCCAGCGGGCCGGTGTTTTGCGCCGGCCACGACCTGCGCGAGTTGCGCGGCGAGCCAAACGAGGCCGCC
CATGCCGCCCTGTTCGCCCAATGCTCGCGCCTGATGACAACCATCGTCCGCCTGCCCAAGCCGGTGATCGCCCGGGTTCA
GGGGCTGGCGACGGCGGCGGGCTGCCAGCTTGTCGCCTCGTGCGATCTGGCGGTGGCGGCGCGCCGCGCCAGCTTCGCCA
CCCCCGGCGTCACCATCGGCCTGTTCTGCTCGACTCCGATGGTCGCCCTGTCGCGCGCCGTCGGCCGCAAGACGGCGATG
GAGATGCTGTTGACCGGCCGGCCGCTCGACGCCGAAGAAGCCCTGGCCCGCGGGTTGATCACCCAGGTGGTCGAGGAGGC
CGAACTGGCCGCCGCCACCGACGCCCTGGCGCGGATCCTCGCCGAGAAATCGCCGCTGACCCTGGCCATCGGCAAAGAGG
CCTTTTACCAGCAGATCGAGCTCCCCCTCGACGAGGCCTATGCCTATGCCAGCGCCGTGATGACCCGAAACATGCAAACC
AAAGACGCCGCCGAAGGCATCGACGCCTTCCTTGAAAAACGCCCGCCCCGCTGGACTGGACAATGA

Upstream 100 bases:

>100_bases
ACCAAATCAAAGACTCGCAGCCGTTTCCTGAACAATATCGACAACACGGCTCCGGCCAGGATTTCCCCCTGGCCGACCCG
GGCGCAAGGATCGGACAGCC

Downstream 100 bases:

>100_bases
CCGCCCCTTTGCCGATGCCCCGCCCGTCGTCCCGTAAGCTCGGAGTGATTTTTGTGCTGCTCGCCCCCCTGCTGCTGGGC
GCTTGCGCCGGCAATACGGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTVPPPLLESLTVDGVRTLTLNRPQARNALSKALMAALQDALDQAAEDPAVRVVVIAASGPVFCAGHDLRELRGEPNEAA
HAALFAQCSRLMTTIVRLPKPVIARVQGLATAAGCQLVASCDLAVAARRASFATPGVTIGLFCSTPMVALSRAVGRKTAM
EMLLTGRPLDAEEALARGLITQVVEEAELAAATDALARILAEKSPLTLAIGKEAFYQQIELPLDEAYAYASAVMTRNMQT
KDAAEGIDAFLEKRPPRWTGQ

Sequences:

>Translated_261_residues
MTVPPPLLESLTVDGVRTLTLNRPQARNALSKALMAALQDALDQAAEDPAVRVVVIAASGPVFCAGHDLRELRGEPNEAA
HAALFAQCSRLMTTIVRLPKPVIARVQGLATAAGCQLVASCDLAVAARRASFATPGVTIGLFCSTPMVALSRAVGRKTAM
EMLLTGRPLDAEEALARGLITQVVEEAELAAATDALARILAEKSPLTLAIGKEAFYQQIELPLDEAYAYASAVMTRNMQT
KDAAEGIDAFLEKRPPRWTGQ
>Mature_260_residues
TVPPPLLESLTVDGVRTLTLNRPQARNALSKALMAALQDALDQAAEDPAVRVVVIAASGPVFCAGHDLRELRGEPNEAAH
AALFAQCSRLMTTIVRLPKPVIARVQGLATAAGCQLVASCDLAVAARRASFATPGVTIGLFCSTPMVALSRAVGRKTAME
MLLTGRPLDAEEALARGLITQVVEEAELAAATDALARILAEKSPLTLAIGKEAFYQQIELPLDEAYAYASAVMTRNMQTK
DAAEGIDAFLEKRPPRWTGQ

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=50.6024096385542, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=33.2, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI4502327, Length=253, Percent_Identity=33.596837944664, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI4757966, Length=247, Percent_Identity=29.5546558704453, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI51538581, Length=247, Percent_Identity=29.5546558704453, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI25453481, Length=247, Percent_Identity=29.5546558704453, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI51538573, Length=247, Percent_Identity=29.5546558704453, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI4757968, Length=247, Percent_Identity=29.5546558704453, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI49355787, Length=247, Percent_Identity=29.5546558704453, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI70995211, Length=268, Percent_Identity=26.865671641791, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI221136756, Length=246, Percent_Identity=29.2682926829268, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI221136753, Length=246, Percent_Identity=29.2682926829268, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI221307494, Length=246, Percent_Identity=29.2682926829268, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI150378541, Length=253, Percent_Identity=30.4347826086957, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI260274832, Length=262, Percent_Identity=25.9541984732824, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI45643119, Length=262, Percent_Identity=25.9541984732824, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260275230, Length=262, Percent_Identity=25.9541984732824, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI116235487, Length=163, Percent_Identity=32.5153374233129, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI37594471, Length=231, Percent_Identity=29.4372294372294, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI37594469, Length=231, Percent_Identity=29.4372294372294, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI68989263, Length=159, Percent_Identity=29.559748427673, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI20127408, Length=175, Percent_Identity=28, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157694516, Length=178, Percent_Identity=24.1573033707865, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI213417737, Length=178, Percent_Identity=24.1573033707865, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=247, Percent_Identity=37.246963562753, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=34.8837209302326, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=32.319391634981, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=35.5731225296443, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1788597, Length=255, Percent_Identity=30.9803921568627, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788682, Length=194, Percent_Identity=29.8969072164948, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1790281, Length=191, Percent_Identity=26.1780104712042, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=245, Percent_Identity=43.6734693877551, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=33.6065573770492, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25145438, Length=251, Percent_Identity=32.2709163346614, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=258, Percent_Identity=29.0697674418605, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=240, Percent_Identity=28.75, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=202, Percent_Identity=29.7029702970297, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=272, Percent_Identity=27.5735294117647, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=258, Percent_Identity=28.6821705426357, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=207, Percent_Identity=29.4685990338164, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=219, Percent_Identity=24.6575342465753, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=216, Percent_Identity=26.8518518518519, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922422, Length=248, Percent_Identity=41.5322580645161, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=33.7301587301587, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=33.7301587301587, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=30.7086614173228, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=27.7777777777778, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24654903, Length=199, Percent_Identity=27.6381909547739, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24650670, Length=167, Percent_Identity=31.7365269461078, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21357171, Length=191, Percent_Identity=30.3664921465969, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583165, Length=210, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27657; Mature: 27526

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPPPLLESLTVDGVRTLTLNRPQARNALSKALMAALQDALDQAAEDPAVRVVVIAASG
CCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
PVFCAGHDLRELRGEPNEAAHAALFAQCSRLMTTIVRLPKPVIARVQGLATAAGCQLVAS
CEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
CDLAVAARRASFATPGVTIGLFCSTPMVALSRAVGRKTAMEMLLTGRPLDAEEALARGLI
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TQVVEEAELAAATDALARILAEKSPLTLAIGKEAFYQQIELPLDEAYAYASAVMTRNMQT
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCH
KDAAEGIDAFLEKRPPRWTGQ
HHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVPPPLLESLTVDGVRTLTLNRPQARNALSKALMAALQDALDQAAEDPAVRVVVIAASG
CCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
PVFCAGHDLRELRGEPNEAAHAALFAQCSRLMTTIVRLPKPVIARVQGLATAAGCQLVAS
CEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
CDLAVAARRASFATPGVTIGLFCSTPMVALSRAVGRKTAMEMLLTGRPLDAEEALARGLI
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TQVVEEAELAAATDALARILAEKSPLTLAIGKEAFYQQIELPLDEAYAYASAVMTRNMQT
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCH
KDAAEGIDAFLEKRPPRWTGQ
HHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA