Click here to switch to the map view.

The map label for this gene is nolF [H]

Identifier: 83593430

GI number: 83593430

Start: 2424229

End: 2425293

Strand: Reverse

Name: nolF [H]

Synonym: Rru_A2095

Alternate gene names: 83593430

Gene position: 2425293-2424229 (Counterclockwise)

Preceding gene: 83593431

Following gene: 83593429

Centisome position: 55.72

GC content: 70.33

Gene sequence:

>1065_bases
ATGAGGATTCTGCGGCAGGCCGTTCTGGCGACCGTCCTTGTTCTGGGGGGGGGCGCGGCCTTGGTCGGGCGATCGTGGCT
GATGCCCGATGCCGATGGCAAGCAGAGCGCCCCCGCCCTGCGGGCCCTGCCGGTCGAGGTGGCGGCGGTGCGGCGGGCGC
GTGTGGCCGATCGGGTGGAAGCCGTGGGCACCACCCGCGCCCATCAGGCGATCGCCGTGGTCCCTTTGGCCTCGGGCCGC
ATCGTTGAGATGCCCTTTCCCCCCGGTCGCAAGGTCAATAAGGGCGATGTTCTCGTCCGTCTGGACGATGCCGCCCAGCG
CGCCGATGTCGAGGAGGCCGAGGCGACGCTGACCCAGGTCAGCCGGGCTTTGGAGCGCGCGCGGTCGCTGCGGCGGACCC
AGGCGGTGGCCCAGGCGACGGTCGATGACCTGCAAAGCAACCGCGCCGCCGCCGCCGCCCGCCTGGATCGAGCCCACAAG
GATCTGGCCGACCGGGTGATCGCCGCGCCCTTCGCCGGCATCGTCGGCTTCCGCGAGCTTGATGTCGGCGCCCGGGTTTC
CGAGGGCGAACGCCTGACCACCCTTGATGATCTGTCCAGCGTCGATGTCGAATTCGCCGTGCCCGAGATCCATTTCGGCC
GCCTGCGCCCGGGCATGGCCATCGAGGCGACTTCGCAGGCCTTTCCCGGACGGATCTTCACCGGCGCCATTGATCGCATC
GATAGTCGCATTGGCGAGGTGTCGCGGGCCTTCCGCGTGCGCGCCACCCTGCCCAACCCCCAGGGCCTGTTGCCGTCGGG
GTTGTTCCTCAATGTCTCCATCGTCATCGAGGAGCGCCCGGCCCTGATGGTGCCCGAAGAGGCCATCCAAGCTGAAGCCG
GGGGCAGCGTGGTCTATGTGATCCAGGGCGGCGCGGTGCGACGCCAGCCGATCGTTTTGGGCCAGCGCACCGGCACCGCC
GTGGAGGTGCGCGACGGCTTGGCCGAGGGCGATCAGGTGGTGAGCCGGGGCATCCAGCGCCTGCGCGACGGCTCGCCGGT
GACGGTGATCGGCTCGGCCGGTTAG

Upstream 100 bases:

>100_bases
CACCCAAAGCCGATGCGTAGAGACCAACGAGCATTGACCAAGGGCAAGGCGCCGCCTTTTTTTCGAAAAAGGCGGGCCGG
GCGGAACAAAAGGAAGGGTG

Downstream 100 bases:

>100_bases
AGCGATGGGCGTTAACCCTAAGTCCTCGCGCATCGCTTTAACAGACTGATATTGACTCAAATCCTTGTCGCATTCTGATG
CGGAATGCTTGCGGGATTTG

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRILRQAVLATVLVLGGGAALVGRSWLMPDADGKQSAPALRALPVEVAAVRRARVADRVEAVGTTRAHQAIAVVPLASGR
IVEMPFPPGRKVNKGDVLVRLDDAAQRADVEEAEATLTQVSRALERARSLRRTQAVAQATVDDLQSNRAAAAARLDRAHK
DLADRVIAAPFAGIVGFRELDVGARVSEGERLTTLDDLSSVDVEFAVPEIHFGRLRPGMAIEATSQAFPGRIFTGAIDRI
DSRIGEVSRAFRVRATLPNPQGLLPSGLFLNVSIVIEERPALMVPEEAIQAEAGGSVVYVIQGGAVRRQPIVLGQRTGTA
VEVRDGLAEGDQVVSRGIQRLRDGSPVTVIGSAG

Sequences:

>Translated_354_residues
MRILRQAVLATVLVLGGGAALVGRSWLMPDADGKQSAPALRALPVEVAAVRRARVADRVEAVGTTRAHQAIAVVPLASGR
IVEMPFPPGRKVNKGDVLVRLDDAAQRADVEEAEATLTQVSRALERARSLRRTQAVAQATVDDLQSNRAAAAARLDRAHK
DLADRVIAAPFAGIVGFRELDVGARVSEGERLTTLDDLSSVDVEFAVPEIHFGRLRPGMAIEATSQAFPGRIFTGAIDRI
DSRIGEVSRAFRVRATLPNPQGLLPSGLFLNVSIVIEERPALMVPEEAIQAEAGGSVVYVIQGGAVRRQPIVLGQRTGTA
VEVRDGLAEGDQVVSRGIQRLRDGSPVTVIGSAG
>Mature_354_residues
MRILRQAVLATVLVLGGGAALVGRSWLMPDADGKQSAPALRALPVEVAAVRRARVADRVEAVGTTRAHQAIAVVPLASGR
IVEMPFPPGRKVNKGDVLVRLDDAAQRADVEEAEATLTQVSRALERARSLRRTQAVAQATVDDLQSNRAAAAARLDRAHK
DLADRVIAAPFAGIVGFRELDVGARVSEGERLTTLDDLSSVDVEFAVPEIHFGRLRPGMAIEATSQAFPGRIFTGAIDRI
DSRIGEVSRAFRVRATLPNPQGLLPSGLFLNVSIVIEERPALMVPEEAIQAEAGGSVVYVIQGGAVRRQPIVLGQRTGTA
VEVRDGLAEGDQVVSRGIQRLRDGSPVTVIGSAG

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=332, Percent_Identity=25.3012048192771, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789929, Length=327, Percent_Identity=25.9938837920489, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789665, Length=342, Percent_Identity=26.0233918128655, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786668, Length=371, Percent_Identity=26.4150943396226, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081796, Length=366, Percent_Identity=25.6830601092896, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37544; Mature: 37544

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILRQAVLATVLVLGGGAALVGRSWLMPDADGKQSAPALRALPVEVAAVRRARVADRVE
CHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHH
AVGTTRAHQAIAVVPLASGRIVEMPFPPGRKVNKGDVLVRLDDAAQRADVEEAEATLTQV
HHCCCHHCCEEEEEEECCCCEEECCCCCCCCCCCCCEEEEECCHHHHCCHHHHHHHHHHH
SRALERARSLRRTQAVAQATVDDLQSNRAAAAARLDRAHKDLADRVIAAPFAGIVGFREL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
DVGARVSEGERLTTLDDLSSVDVEFAVPEIHFGRLRPGMAIEATSQAFPGRIFTGAIDRI
CCCCCCCCCCEEEEHHHHCCCCEEEECCCEECCCCCCCCEEEECCCCCCCCHHHHHHHHH
DSRIGEVSRAFRVRATLPNPQGLLPSGLFLNVSIVIEERPALMVPEEAIQAEAGGSVVYV
HHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEEEEEECCCCEECCHHHHHHCCCCCEEEE
IQGGAVRRQPIVLGQRTGTAVEVRDGLAEGDQVVSRGIQRLRDGSPVTVIGSAG
EECCCEECCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
>Mature Secondary Structure
MRILRQAVLATVLVLGGGAALVGRSWLMPDADGKQSAPALRALPVEVAAVRRARVADRVE
CHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHH
AVGTTRAHQAIAVVPLASGRIVEMPFPPGRKVNKGDVLVRLDDAAQRADVEEAEATLTQV
HHCCCHHCCEEEEEEECCCCEEECCCCCCCCCCCCCEEEEECCHHHHCCHHHHHHHHHHH
SRALERARSLRRTQAVAQATVDDLQSNRAAAAARLDRAHKDLADRVIAAPFAGIVGFREL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
DVGARVSEGERLTTLDDLSSVDVEFAVPEIHFGRLRPGMAIEATSQAFPGRIFTGAIDRI
CCCCCCCCCCEEEEHHHHCCCCEEEECCCEECCCCCCCCEEEECCCCCCCCHHHHHHHHH
DSRIGEVSRAFRVRATLPNPQGLLPSGLFLNVSIVIEERPALMVPEEAIQAEAGGSVVYV
HHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEEEEEECCCCEECCHHHHHHCCCCCEEEE
IQGGAVRRQPIVLGQRTGTAVEVRDGLAEGDQVVSRGIQRLRDGSPVTVIGSAG
EECCCEECCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]