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The map label for this gene is puuA [H]

Identifier: 83593414

GI number: 83593414

Start: 2407957

End: 2409321

Strand: Reverse

Name: puuA [H]

Synonym: Rru_A2079

Alternate gene names: 83593414

Gene position: 2409321-2407957 (Counterclockwise)

Preceding gene: 83593418

Following gene: 83593412

Centisome position: 55.35

GC content: 61.76

Gene sequence:

>1365_bases
ATGAAAACCGCAGAACCGCAGACTATCGACGATCTCGAACGGTGGATCAAAAGCCGTGGCATCACCGAGGTCGAATGTCT
CGTGCCCGATCTATCGGGAATCCCCCGGGGCAAGATCCTGCCCGCGCGCAAATTCCTCTCGTGCTTGCGCGACCGGGGGT
TGCGCTTGCCCGAGTCGGTCTTCACCCAGACGGTGACCGGCGAATACCCCGAGGATGATCCGGTCGATATCGCCGATTCG
GACGTTTACCTGCGCCCCGACCCCCGGACCATCCGCGTCGTCCCCTGGTACGAGGAGCCGACGGCCCAGGTGATCAACGA
TTGCTTCCACCGCGATCGCTCCGAGGTGCATTGCGCCCCCCGTCAGGTGTTGCGCAACATCCTGGCGCTTTATGAAAAGC
AGGGGTGGAAGCCGGTGGTCGCGCCGGAACTGGAATTCTATCTGGTGCGGATCAACGCCGATCCCGATTATCCGCTGGAA
CCGCCGATCGGCCGCTCGGGCCGGGCCGAAACCGGGCGTCAGGCCTATGGCATCGACGCCGTCAACGAATTCGATCCGTT
GTTCGAGGACATCTACGATTACGCCGAGGAACAGAACCTCGACCTTGATACCCTGCACCATGAATCGGGCTCGGCGCAGA
TGGAGATCAATTTTCTCCATGGCGATCCGATGGAACTGGCCGATCAGGTGTTCTTGTTCAAGCGCACGGTGCGTCAGGCC
GCCCTTCGCCATAACGTCTATGCCACCTTCATGGCCAAGCCGCTGGCCGGTCAGCCGGGCAGCGCCATGCACCTGCACCA
AAGCATCACCGATGCCGAAAGCGGGCGGAATATCCTGGTCAAGAAGAACGGCGAGGATTCGGATCTGTTCCGCTGGCACA
TCGGCGGCCTGCAGAAATACCTGCCCCACTGCATGCTGATGCTGGCGCCCAACGTCAACAGCTACCGGCGCTTCCTGCCC
GATCAGTCGGCGCCGATCAACATGCATTGGGGCCATGACAACCGCACGGTGTCGTTGCGCGTGCCCTATGCCGACGCCAG
CGCCCGCCGGGTTGAAAACCGCCTGTCGGGGGCCGATGCCAACCCCTATCTGGCGATCGCCGCCTCATTGGCCTGCGGCT
ATCTGGGGATGATCGAAAAGATCGAACCCACCGCGCCGATCACCGGCAGCGCCTATACCCGTGCCCACAACCTGCCGCGC
CATCTGCCCGATGCCATCGAACGCTTCGAACGGGCCCGGCCGCTGCATGAATTGCTCAACGCCGATTTCGTCCGCGTCTA
CGCCTCGGTGAAATGGGCGGAATGGGATGCCTACCAGCATGTGATTTCAAGCTGGGAACGCGAATACTTGCTGCTTAACG
TCTGA

Upstream 100 bases:

>100_bases
AATGGCCGTGACTTAAACCTACGCCGCTCGGCCGGGCGAAAGCAACAGCCCACCTTTGGTGGCGGCTTCCCGCCTCCTAA
CCTTCTAATCTGGCCCTCAC

Downstream 100 bases:

>100_bases
CGTCGGCCCCGCGCCGCCCTGCCGATCTCGCCTCGGTCGGGACGGCGGCGCGGGCCTTGGCCTTACAGGGCGCGGACCTT
GTCGAGGAAGGTGGTCACCA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKTAEPQTIDDLERWIKSRGITEVECLVPDLSGIPRGKILPARKFLSCLRDRGLRLPESVFTQTVTGEYPEDDPVDIADS
DVYLRPDPRTIRVVPWYEEPTAQVINDCFHRDRSEVHCAPRQVLRNILALYEKQGWKPVVAPELEFYLVRINADPDYPLE
PPIGRSGRAETGRQAYGIDAVNEFDPLFEDIYDYAEEQNLDLDTLHHESGSAQMEINFLHGDPMELADQVFLFKRTVRQA
ALRHNVYATFMAKPLAGQPGSAMHLHQSITDAESGRNILVKKNGEDSDLFRWHIGGLQKYLPHCMLMLAPNVNSYRRFLP
DQSAPINMHWGHDNRTVSLRVPYADASARRVENRLSGADANPYLAIAASLACGYLGMIEKIEPTAPITGSAYTRAHNLPR
HLPDAIERFERARPLHELLNADFVRVYASVKWAEWDAYQHVISSWEREYLLLNV

Sequences:

>Translated_454_residues
MKTAEPQTIDDLERWIKSRGITEVECLVPDLSGIPRGKILPARKFLSCLRDRGLRLPESVFTQTVTGEYPEDDPVDIADS
DVYLRPDPRTIRVVPWYEEPTAQVINDCFHRDRSEVHCAPRQVLRNILALYEKQGWKPVVAPELEFYLVRINADPDYPLE
PPIGRSGRAETGRQAYGIDAVNEFDPLFEDIYDYAEEQNLDLDTLHHESGSAQMEINFLHGDPMELADQVFLFKRTVRQA
ALRHNVYATFMAKPLAGQPGSAMHLHQSITDAESGRNILVKKNGEDSDLFRWHIGGLQKYLPHCMLMLAPNVNSYRRFLP
DQSAPINMHWGHDNRTVSLRVPYADASARRVENRLSGADANPYLAIAASLACGYLGMIEKIEPTAPITGSAYTRAHNLPR
HLPDAIERFERARPLHELLNADFVRVYASVKWAEWDAYQHVISSWEREYLLLNV
>Mature_454_residues
MKTAEPQTIDDLERWIKSRGITEVECLVPDLSGIPRGKILPARKFLSCLRDRGLRLPESVFTQTVTGEYPEDDPVDIADS
DVYLRPDPRTIRVVPWYEEPTAQVINDCFHRDRSEVHCAPRQVLRNILALYEKQGWKPVVAPELEFYLVRINADPDYPLE
PPIGRSGRAETGRQAYGIDAVNEFDPLFEDIYDYAEEQNLDLDTLHHESGSAQMEINFLHGDPMELADQVFLFKRTVRQA
ALRHNVYATFMAKPLAGQPGSAMHLHQSITDAESGRNILVKKNGEDSDLFRWHIGGLQKYLPHCMLMLAPNVNSYRRFLP
DQSAPINMHWGHDNRTVSLRVPYADASARRVENRLSGADANPYLAIAASLACGYLGMIEKIEPTAPITGSAYTRAHNLPR
HLPDAIERFERARPLHELLNADFVRVYASVKWAEWDAYQHVISSWEREYLLLNV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=422, Percent_Identity=23.696682464455, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI87081870, Length=448, Percent_Identity=33.9285714285714, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1790301, Length=387, Percent_Identity=26.6149870801034, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 51764; Mature: 51764

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAEPQTIDDLERWIKSRGITEVECLVPDLSGIPRGKILPARKFLSCLRDRGLRLPESV
CCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHH
FTQTVTGEYPEDDPVDIADSDVYLRPDPRTIRVVPWYEEPTAQVINDCFHRDRSEVHCAP
HHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCH
RQVLRNILALYEKQGWKPVVAPELEFYLVRINADPDYPLEPPIGRSGRAETGRQAYGIDA
HHHHHHHHHHHHHCCCCCEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
VNEFDPLFEDIYDYAEEQNLDLDTLHHESGSAQMEINFLHGDPMELADQVFLFKRTVRQA
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
ALRHNVYATFMAKPLAGQPGSAMHLHQSITDAESGRNILVKKNGEDSDLFRWHIGGLQKY
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHH
LPHCMLMLAPNVNSYRRFLPDQSAPINMHWGHDNRTVSLRVPYADASARRVENRLSGADA
HHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCC
NPYLAIAASLACGYLGMIEKIEPTAPITGSAYTRAHNLPRHLPDAIERFERARPLHELLN
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCHHHHHHC
ADFVRVYASVKWAEWDAYQHVISSWEREYLLLNV
CHHEEHHHHCCHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKTAEPQTIDDLERWIKSRGITEVECLVPDLSGIPRGKILPARKFLSCLRDRGLRLPESV
CCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHH
FTQTVTGEYPEDDPVDIADSDVYLRPDPRTIRVVPWYEEPTAQVINDCFHRDRSEVHCAP
HHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCH
RQVLRNILALYEKQGWKPVVAPELEFYLVRINADPDYPLEPPIGRSGRAETGRQAYGIDA
HHHHHHHHHHHHHCCCCCEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
VNEFDPLFEDIYDYAEEQNLDLDTLHHESGSAQMEINFLHGDPMELADQVFLFKRTVRQA
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
ALRHNVYATFMAKPLAGQPGSAMHLHQSITDAESGRNILVKKNGEDSDLFRWHIGGLQKY
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHH
LPHCMLMLAPNVNSYRRFLPDQSAPINMHWGHDNRTVSLRVPYADASARRVENRLSGADA
HHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCC
NPYLAIAASLACGYLGMIEKIEPTAPITGSAYTRAHNLPRHLPDAIERFERARPLHELLN
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCHHHHHHC
ADFVRVYASVKWAEWDAYQHVISSWEREYLLLNV
CHHEEHHHHCCHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]