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The map label for this gene is oprM [H]

Identifier: 83593387

GI number: 83593387

Start: 2374586

End: 2376085

Strand: Direct

Name: oprM [H]

Synonym: Rru_A2052

Alternate gene names: 83593387

Gene position: 2374586-2376085 (Clockwise)

Preceding gene: 83593386

Following gene: 83593388

Centisome position: 54.55

GC content: 65.33

Gene sequence:

>1500_bases
ATGCCCCGTAACTCTCTTTCGCAGCGGACCCCGATCATGCGCTCCTCCTTGATGTCTTGCGTTGCCGTTCTGGCGCTGCT
CGGGGGATGCTCGTTCATCCCCGATTACCAGCGGCCGGCGGCGCCGGTTCCCACCGCCTGGCCCGATGGTCCGGCCTATG
ACCAGGCCCAGGCCGGGGGCGAGACCATCGCCACCCTCGGCTGGCGGGAGTTCTTCCGCGACCCGGCGCTCCAGCGCCTG
ATCGCCCAGGCCCTGGAGCATAATCGCGACCTGCGCTCGGCCGCCCTGACGGTGGAAAGCGCCCGGGCCACCTATCGTAT
CGAACGGGCCGATCTGCTGCCCACCGTCAACGGCGGCACCGACCTAACCCACCAACGCACGCCGCGCACGGCCACCCAGA
CCGGCAAGGCCCAGACGACGACCAATTACTCGGCCAATATCGGCACCACGTCCTATGAGATCGATTTCTTTGGCCGTATC
CGCAGCCTGGAGGAAGAGGCGCTCGAGCAATATTTCGCCACCGAGGAGGCCCGGCGAAGCGTTCAGATCGCCTTGATCGC
CGAGGTTGCCAACGCCTATATCACCCTGCTGGCCGACCGAACCTTGTTGCAGCTCACCGAAGAGACCCTGAACACCCGCA
TCGAATCCTTCAATCTGACCCAGCAAAGCTTCGATCGCGGCGTCGGCACCCGCCTGGATGTCACCCAGGCCCAGACCACG
GTGGAAACCGCCCGGGCCAACCGCGCCATCTATACCCGCTATGTCGCCCAGGATATCAACGCCCTGCGCCTGCTGGTCGG
CGATCAGCTTGATGCGAACCTGCTGGCCGGCACGCCGCCGCTCGATCTTGGGCGGTTTTTCCCCAATGTTCCCGCCGGTC
TGCCCTCGGACCTGCTGCTGCGCCGGCCCGATATCCTGGAAGCCGAGCACAAGCTGGTGGCGGCCAACGCCAATATCGGC
GCGGCGCGGGCGGCCTTCTGGCCGAAGATCTCGCTGACGGGCAATCTGGGAACGGCCAGCGCCTCGCTCGGCGATCTGTT
TAGCAGCGGCTCCCTGGCCTGGAGCGTCGGCCCCTCGCTCAGCGTGCCGCTGTTTGATTTCTGGCGCAACGAGGCGACCT
TGGAAAGCGCCAAGGTCCAGCGTGAAATCGCCGTCGCCGCCTACGAGAAATCGATCCAGACCGCCTTCCGCGAAGTCGCC
GACGCCCTGGCGGCCAAGGGAACCCTGGGCGATCAGTTGCGCGCCCAGCAAGATCTGGTCGCCGCCACCCAGGAAAGCTA
CGCCCTGTCGCGCAACCGCTATGACCAGGGCATCGACAATTACCTCTCGACCCTGGATTCCCAGCGCTCGCTCTATGCGG
CCCAGCAAGATCTGATCAGCGTGCGGGCCAACCAGCTCACCAATTACATCACCCTCTATAAGGTGCTGGGCGGCGGCAGC
TTCGCCACTTCGCCCTCCGAACTGCCCAATCCGGCGGCCAGCGTCAGCACCTTTCCCTAA

Upstream 100 bases:

>100_bases
ATTTTCTTCGTACCCTTGTTCTTCATCCTGATCTTGCGCGCCTTCACCAAAAAGACCCCATCGACGCCCCCCGCGCCCCC
CTCCCCCGGAGCCGCCGCCG

Downstream 100 bases:

>100_bases
CCCCCGAAACTCCCTCCCCTCACGAAGATCGCCGAACCTTGGTGTTCCGTGGGGGCGAGGGTCTTTCCCCCGGCTTGGCT
TTTTCCCCGAGCGAAGCTGG

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MPRNSLSQRTPIMRSSLMSCVAVLALLGGCSFIPDYQRPAAPVPTAWPDGPAYDQAQAGGETIATLGWREFFRDPALQRL
IAQALEHNRDLRSAALTVESARATYRIERADLLPTVNGGTDLTHQRTPRTATQTGKAQTTTNYSANIGTTSYEIDFFGRI
RSLEEEALEQYFATEEARRSVQIALIAEVANAYITLLADRTLLQLTEETLNTRIESFNLTQQSFDRGVGTRLDVTQAQTT
VETARANRAIYTRYVAQDINALRLLVGDQLDANLLAGTPPLDLGRFFPNVPAGLPSDLLLRRPDILEAEHKLVAANANIG
AARAAFWPKISLTGNLGTASASLGDLFSSGSLAWSVGPSLSVPLFDFWRNEATLESAKVQREIAVAAYEKSIQTAFREVA
DALAAKGTLGDQLRAQQDLVAATQESYALSRNRYDQGIDNYLSTLDSQRSLYAAQQDLISVRANQLTNYITLYKVLGGGS
FATSPSELPNPAASVSTFP

Sequences:

>Translated_499_residues
MPRNSLSQRTPIMRSSLMSCVAVLALLGGCSFIPDYQRPAAPVPTAWPDGPAYDQAQAGGETIATLGWREFFRDPALQRL
IAQALEHNRDLRSAALTVESARATYRIERADLLPTVNGGTDLTHQRTPRTATQTGKAQTTTNYSANIGTTSYEIDFFGRI
RSLEEEALEQYFATEEARRSVQIALIAEVANAYITLLADRTLLQLTEETLNTRIESFNLTQQSFDRGVGTRLDVTQAQTT
VETARANRAIYTRYVAQDINALRLLVGDQLDANLLAGTPPLDLGRFFPNVPAGLPSDLLLRRPDILEAEHKLVAANANIG
AARAAFWPKISLTGNLGTASASLGDLFSSGSLAWSVGPSLSVPLFDFWRNEATLESAKVQREIAVAAYEKSIQTAFREVA
DALAAKGTLGDQLRAQQDLVAATQESYALSRNRYDQGIDNYLSTLDSQRSLYAAQQDLISVRANQLTNYITLYKVLGGGS
FATSPSELPNPAASVSTFP
>Mature_498_residues
PRNSLSQRTPIMRSSLMSCVAVLALLGGCSFIPDYQRPAAPVPTAWPDGPAYDQAQAGGETIATLGWREFFRDPALQRLI
AQALEHNRDLRSAALTVESARATYRIERADLLPTVNGGTDLTHQRTPRTATQTGKAQTTTNYSANIGTTSYEIDFFGRIR
SLEEEALEQYFATEEARRSVQIALIAEVANAYITLLADRTLLQLTEETLNTRIESFNLTQQSFDRGVGTRLDVTQAQTTV
ETARANRAIYTRYVAQDINALRLLVGDQLDANLLAGTPPLDLGRFFPNVPAGLPSDLLLRRPDILEAEHKLVAANANIGA
ARAAFWPKISLTGNLGTASASLGDLFSSGSLAWSVGPSLSVPLFDFWRNEATLESAKVQREIAVAAYEKSIQTAFREVAD
ALAAKGTLGDQLRAQQDLVAATQESYALSRNRYDQGIDNYLSTLDSQRSLYAAQQDLISVRANQLTNYITLYKVLGGGSF
ATSPSELPNPAASVSTFP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=466, Percent_Identity=40.343347639485, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI2367348, Length=481, Percent_Identity=24.1164241164241, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54314; Mature: 54183

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRNSLSQRTPIMRSSLMSCVAVLALLGGCSFIPDYQRPAAPVPTAWPDGPAYDQAQAGG
CCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
ETIATLGWREFFRDPALQRLIAQALEHNRDLRSAALTVESARATYRIERADLLPTVNGGT
CHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEHHHCCCCCCCCC
DLTHQRTPRTATQTGKAQTTTNYSANIGTTSYEIDFFGRIRSLEEEALEQYFATEEARRS
CCCCCCCCCCHHCCCCCEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
VQIALIAEVANAYITLLADRTLLQLTEETLNTRIESFNLTQQSFDRGVGTRLDVTQAQTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEHHHHHHH
VETARANRAIYTRYVAQDINALRLLVGDQLDANLLAGTPPLDLGRFFPNVPAGLPSDLLL
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHH
RRPDILEAEHKLVAANANIGAARAAFWPKISLTGNLGTASASLGDLFSSGSLAWSVGPSL
CCCCHHCCCCEEEEECCCCCCHHHHHCCCEEEECCCCCCCHHHHHHHCCCCEEEECCCCC
SVPLFDFWRNEATLESAKVQREIAVAAYEKSIQTAFREVADALAAKGTLGDQLRAQQDLV
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AATQESYALSRNRYDQGIDNYLSTLDSQRSLYAAQQDLISVRANQLTNYITLYKVLGGGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FATSPSELPNPAASVSTFP
CCCCCHHCCCCCCCCCCCC
>Mature Secondary Structure 
PRNSLSQRTPIMRSSLMSCVAVLALLGGCSFIPDYQRPAAPVPTAWPDGPAYDQAQAGG
CCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
ETIATLGWREFFRDPALQRLIAQALEHNRDLRSAALTVESARATYRIERADLLPTVNGGT
CHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEHHHCCCCCCCCC
DLTHQRTPRTATQTGKAQTTTNYSANIGTTSYEIDFFGRIRSLEEEALEQYFATEEARRS
CCCCCCCCCCHHCCCCCEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
VQIALIAEVANAYITLLADRTLLQLTEETLNTRIESFNLTQQSFDRGVGTRLDVTQAQTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEHHHHHHH
VETARANRAIYTRYVAQDINALRLLVGDQLDANLLAGTPPLDLGRFFPNVPAGLPSDLLL
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHH
RRPDILEAEHKLVAANANIGAARAAFWPKISLTGNLGTASASLGDLFSSGSLAWSVGPSL
CCCCHHCCCCEEEEECCCCCCHHHHHCCCEEEECCCCCCCHHHHHHHCCCCEEEECCCCC
SVPLFDFWRNEATLESAKVQREIAVAAYEKSIQTAFREVADALAAKGTLGDQLRAQQDLV
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AATQESYALSRNRYDQGIDNYLSTLDSQRSLYAAQQDLISVRANQLTNYITLYKVLGGGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FATSPSELPNPAASVSTFP
CCCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]