Click here to switch to the map view.

The map label for this gene is paaK [H]

Identifier: 83593311

GI number: 83593311

Start: 2286031

End: 2287344

Strand: Reverse

Name: paaK [H]

Synonym: Rru_A1976

Alternate gene names: 83593311

Gene position: 2287344-2286031 (Counterclockwise)

Preceding gene: 83593312

Following gene: 83593310

Centisome position: 52.55

GC content: 66.74

Gene sequence:

>1314_bases
ATGCATATTTCCAACGATGGCTATATGTTCGCGAAAGATCGCGAGACGATGCCGCGCGAGCACCTCGCCGGCCTGCAATT
CGCCCGTCTCAAGGACACCCTGACCCGGGTTTACGCCAGCGTTCCCCATTACCGACGGGCTTTCGAGGCCAAGGGGGTCT
CGCCCGAGGATCTGAACACGCTTGAGGATCTGGCGCGGTTCCCCTTCACGGTGAAGGACGACCTGCGCGAGGCCTATCCC
TTCAAGCTGTTCGCCGTGCCGCGCGATCAGGTGGTGCGGCTCCACGCCTCGTCGGGCACCACCGGCTTGCCCACGGTGGT
CGGCTATACCCGCGCCGATATCGACACCTGGGCCGGGCTGATGGCGCGCTGCATCGCCTGCGCCGGTGGCCGGCCGGGCG
ATATCTTGCATAACGCCTATGGCTACGGCCTGTTCACCGGCGGTCTGGGCGCCCATTACGGCGCCGAGGCCCTGGGTTGT
TCCGTGGTGCCGATGTCGGGGGGCAATACGGAAAAGCAGATCCTGCTGCTCAAGGATTTCGGCGCCAATGTCGTGTGCTG
CACGCCATCCTACGCGATCAATCTCGCCGAGGAGGCCGAGCGGCGGGGCGTTGATCTCAAGGGCGGACCCTTGCGGGTGG
GGCTGTTTGGCGCCGAGCCCTGGTGCCAGGGCATGCGCCTGGAACTCGAGGAGCGCCTTGGTATCCGCGCCATGGACGTG
TACGGCCTAAGCGAGATCATGGGGCCGGGCGTCGCGGTCGAATGCGCCGAGCAGGCCGGTCTGCATGGCTGGGAGGATCA
TTTCCTGTTCGAGGTCATCGACCCGGAAACCCTGCGGCCGCTGCCCGTGGGCGAGCAGGGGGAGTTGGTCATCACCACCC
TGACCAAGCAGGCCCTGCCGATGATCCGCTACCGCACCCGCGATATCACCCGGCTGTCGGACGAGCCTTGCCCCTGCGGC
CGCACCCACCGGCGCATCCTGCGGGTGACCGGGCGCAACGACGACATGATGATCATCCGCGGGGTCAATGTGTATCCCTC
GCAGCTGGAAGCGGTGATGGTCGGCCGCCCGGGCCTCGCCCCCCATTACCAGCTGATCTTGCAACGCGACGGCTCGCTGG
ACACCCTGACCGTCGAGGTCGAGGCCCGCGAGGGGCTGCCCTGCGACGACTACGACGCCATCGGCCGCGACCTGGGCCGC
CATATCAAATCCCTGGTCGGCGTCACCGCCCGCATCATCGTGGTGCCCCCGGGCCAGATCGCCCGCTCGCAAGGCAAGGC
CGCCCGGGTGCGCGATCTGCGCAAACAGTATTAG

Upstream 100 bases:

>100_bases
GGCGGCCGCCCATCGGGCGCGCGCCGAGGATGCGGCGGCCGAGTGAGCTTTTTGCGGGCGGCGCGGGGGAAAGGCGCCGC
TGATCTTTGAGGGGGGACCG

Downstream 100 bases:

>100_bases
GGGGGGGCGCAGAAGGGGGCGGCCGAGGCTGTGGCTCAGCCACAGGGGGGCTTTGCCCTCCTTCTGCCCCAGATATCGAA
ACAAGACGCTCACGCTGCTA

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MHISNDGYMFAKDRETMPREHLAGLQFARLKDTLTRVYASVPHYRRAFEAKGVSPEDLNTLEDLARFPFTVKDDLREAYP
FKLFAVPRDQVVRLHASSGTTGLPTVVGYTRADIDTWAGLMARCIACAGGRPGDILHNAYGYGLFTGGLGAHYGAEALGC
SVVPMSGGNTEKQILLLKDFGANVVCCTPSYAINLAEEAERRGVDLKGGPLRVGLFGAEPWCQGMRLELEERLGIRAMDV
YGLSEIMGPGVAVECAEQAGLHGWEDHFLFEVIDPETLRPLPVGEQGELVITTLTKQALPMIRYRTRDITRLSDEPCPCG
RTHRRILRVTGRNDDMMIIRGVNVYPSQLEAVMVGRPGLAPHYQLILQRDGSLDTLTVEVEAREGLPCDDYDAIGRDLGR
HIKSLVGVTARIIVVPPGQIARSQGKAARVRDLRKQY

Sequences:

>Translated_437_residues
MHISNDGYMFAKDRETMPREHLAGLQFARLKDTLTRVYASVPHYRRAFEAKGVSPEDLNTLEDLARFPFTVKDDLREAYP
FKLFAVPRDQVVRLHASSGTTGLPTVVGYTRADIDTWAGLMARCIACAGGRPGDILHNAYGYGLFTGGLGAHYGAEALGC
SVVPMSGGNTEKQILLLKDFGANVVCCTPSYAINLAEEAERRGVDLKGGPLRVGLFGAEPWCQGMRLELEERLGIRAMDV
YGLSEIMGPGVAVECAEQAGLHGWEDHFLFEVIDPETLRPLPVGEQGELVITTLTKQALPMIRYRTRDITRLSDEPCPCG
RTHRRILRVTGRNDDMMIIRGVNVYPSQLEAVMVGRPGLAPHYQLILQRDGSLDTLTVEVEAREGLPCDDYDAIGRDLGR
HIKSLVGVTARIIVVPPGQIARSQGKAARVRDLRKQY
>Mature_437_residues
MHISNDGYMFAKDRETMPREHLAGLQFARLKDTLTRVYASVPHYRRAFEAKGVSPEDLNTLEDLARFPFTVKDDLREAYP
FKLFAVPRDQVVRLHASSGTTGLPTVVGYTRADIDTWAGLMARCIACAGGRPGDILHNAYGYGLFTGGLGAHYGAEALGC
SVVPMSGGNTEKQILLLKDFGANVVCCTPSYAINLAEEAERRGVDLKGGPLRVGLFGAEPWCQGMRLELEERLGIRAMDV
YGLSEIMGPGVAVECAEQAGLHGWEDHFLFEVIDPETLRPLPVGEQGELVITTLTKQALPMIRYRTRDITRLSDEPCPCG
RTHRRILRVTGRNDDMMIIRGVNVYPSQLEAVMVGRPGLAPHYQLILQRDGSLDTLTVEVEAREGLPCDDYDAIGRDLGR
HIKSLVGVTARIIVVPPGQIARSQGKAARVRDLRKQY

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=427, Percent_Identity=57.1428571428571, Blast_Score=470, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 48173; Mature: 48173

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHISNDGYMFAKDRETMPREHLAGLQFARLKDTLTRVYASVPHYRRAFEAKGVSPEDLNT
CEECCCCEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHH
LEDLARFPFTVKDDLREAYPFKLFAVPRDQVVRLHASSGTTGLPTVVGYTRADIDTWAGL
HHHHHHCCCCHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCHHHCCHHHHHHHHHHH
MARCIACAGGRPGDILHNAYGYGLFTGGLGAHYGAEALGCSVVPMSGGNTEKQILLLKDF
HHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCHHHHCCEEEECCCCCCCCEEEEEEEC
GANVVCCTPSYAINLAEEAERRGVDLKGGPLRVGLFGAEPWCQGMRLELEERLGIRAMDV
CCCEEEECCCCEEECHHHHHHCCCCCCCCCEEEEEECCCHHHCCCCEEHHHHCCCEEEEC
YGLSEIMGPGVAVECAEQAGLHGWEDHFLFEVIDPETLRPLPVGEQGELVITTLTKQALP
CCHHHHHCCCCEEEEHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHH
MIRYRTRDITRLSDEPCPCGRTHRRILRVTGRNDDMMIIRGVNVYPSQLEAVMVGRPGLA
HHHHHCCHHHHCCCCCCCCCHHHHEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCC
PHYQLILQRDGSLDTLTVEVEAREGLPCDDYDAIGRDLGRHIKSLVGVTARIIVVPPGQI
HHEEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHH
ARSQGKAARVRDLRKQY
HHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MHISNDGYMFAKDRETMPREHLAGLQFARLKDTLTRVYASVPHYRRAFEAKGVSPEDLNT
CEECCCCEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHH
LEDLARFPFTVKDDLREAYPFKLFAVPRDQVVRLHASSGTTGLPTVVGYTRADIDTWAGL
HHHHHHCCCCHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCHHHCCHHHHHHHHHHH
MARCIACAGGRPGDILHNAYGYGLFTGGLGAHYGAEALGCSVVPMSGGNTEKQILLLKDF
HHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCHHHHCCEEEECCCCCCCCEEEEEEEC
GANVVCCTPSYAINLAEEAERRGVDLKGGPLRVGLFGAEPWCQGMRLELEERLGIRAMDV
CCCEEEECCCCEEECHHHHHHCCCCCCCCCEEEEEECCCHHHCCCCEEHHHHCCCEEEEC
YGLSEIMGPGVAVECAEQAGLHGWEDHFLFEVIDPETLRPLPVGEQGELVITTLTKQALP
CCHHHHHCCCCEEEEHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHH
MIRYRTRDITRLSDEPCPCGRTHRRILRVTGRNDDMMIIRGVNVYPSQLEAVMVGRPGLA
HHHHHCCHHHHCCCCCCCCCHHHHEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCC
PHYQLILQRDGSLDTLTVEVEAREGLPCDDYDAIGRDLGRHIKSLVGVTARIIVVPPGQI
HHEEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHH
ARSQGKAARVRDLRKQY
HHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]