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The map label for this gene is lipA

Identifier: 83593212

GI number: 83593212

Start: 2174144

End: 2175088

Strand: Reverse

Name: lipA

Synonym: Rru_A1877

Alternate gene names: 83593212

Gene position: 2175088-2174144 (Counterclockwise)

Preceding gene: 83593213

Following gene: 83593211

Centisome position: 49.97

GC content: 63.07

Gene sequence:

>945_bases
ATGATGGACACTCCAATCATTCGTCACCCCGAGAAGGTTCGCCGGCCCGACAATCCGTCGCCGCGCAAACCCGAGTGGAT
TCGCGTGAGGGCCCCGGTTTCCCACGAGGCCGCGGAGGTCCGCCAGCTTATGCGCTCGAAGAACCTTTTCACCGTCTGTG
AAGAGGCTGCCTGCCCGAATATCGGCGAGTGCTGGAAGCGCCGTCACGCCACCTTCATGATCCTGGGCGATATCTGCACC
CGGGCCTGCGCCTTTTGTAACGTGCGGACCGGCAAGCCCGGCCATGTCGATGACCAGGAGCCGGTCAATCTGGCCGACTC
CGTGGTGGCGATGGGCCTGAAGCATGTCGTCATCACCTCGGTCGATCGCGATGATCTGGCCGATGGCGGGGCCGGCCATT
TCCATCGCTGCATCACCGAGGTCCGCAGCCGGGCCCCGAGTTGCTCGATCGAGGTACTGACCCCGGATTTCCGTGACAAG
CCGCAAGGCGCGCTTGCCCGGGTGGTCGAAGCCGGTCCCGATGTCTTCAATCACAATCTGGAGACCGTGCCCCGGCTATA
CCCCACCATCCGTCCGGGGGCGCGCTACTTTCATTCCCTGAAGCTTCTCGACCGGGTCAAGACGATCGATCCGGGGGTGT
TCACCAAATCGGGCATCATGGTCGGTCTGGGGGAAACCCGCGAGGAGGTTTTGCAGGTCATGGACGACATGCGTTCGGCC
GGGGTCGATTTCCTGACCATCGGTCAATACCTTCAGCCGACCTTGAAGCATGTCGCCGTCGACCGCTTCGTGACGCCCGA
TGAATTCAAGGACTATGCCGATATCGCCCGGGGCAAGGGTTTCCTTATGGTCGCCTCGTCGCCGCTCACCCGCTCCTCCC
ACCATGCGGACCGCGATTTCGAGGACCTGCGCAAGGCCCGACAGGACGCCGCCGCCACCAAGTGA

Upstream 100 bases:

>100_bases
GATTTGATCCACACCGTCTTCGCCCATCCGACCCTCTCGGAGATGGTGCATGAATCCGTGCTTGACGCTTACGGTCGCGC
CATCCATTTCTAGGGACACC

Downstream 100 bases:

>100_bases
TTCCCTGATCAATGCCCACACACGCCGAGAAACGGTTCTTACCTTACCAGCCCGAACAAATGTACGATCTGGTCGCCGAT
ATCGAAAGTTATCCGCGCTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MMDTPIIRHPEKVRRPDNPSPRKPEWIRVRAPVSHEAAEVRQLMRSKNLFTVCEEAACPNIGECWKRRHATFMILGDICT
RACAFCNVRTGKPGHVDDQEPVNLADSVVAMGLKHVVITSVDRDDLADGGAGHFHRCITEVRSRAPSCSIEVLTPDFRDK
PQGALARVVEAGPDVFNHNLETVPRLYPTIRPGARYFHSLKLLDRVKTIDPGVFTKSGIMVGLGETREEVLQVMDDMRSA
GVDFLTIGQYLQPTLKHVAVDRFVTPDEFKDYADIARGKGFLMVASSPLTRSSHHADRDFEDLRKARQDAAATK

Sequences:

>Translated_314_residues
MMDTPIIRHPEKVRRPDNPSPRKPEWIRVRAPVSHEAAEVRQLMRSKNLFTVCEEAACPNIGECWKRRHATFMILGDICT
RACAFCNVRTGKPGHVDDQEPVNLADSVVAMGLKHVVITSVDRDDLADGGAGHFHRCITEVRSRAPSCSIEVLTPDFRDK
PQGALARVVEAGPDVFNHNLETVPRLYPTIRPGARYFHSLKLLDRVKTIDPGVFTKSGIMVGLGETREEVLQVMDDMRSA
GVDFLTIGQYLQPTLKHVAVDRFVTPDEFKDYADIARGKGFLMVASSPLTRSSHHADRDFEDLRKARQDAAATK
>Mature_314_residues
MMDTPIIRHPEKVRRPDNPSPRKPEWIRVRAPVSHEAAEVRQLMRSKNLFTVCEEAACPNIGECWKRRHATFMILGDICT
RACAFCNVRTGKPGHVDDQEPVNLADSVVAMGLKHVVITSVDRDDLADGGAGHFHRCITEVRSRAPSCSIEVLTPDFRDK
PQGALARVVEAGPDVFNHNLETVPRLYPTIRPGARYFHSLKLLDRVKTIDPGVFTKSGIMVGLGETREEVLQVMDDMRSA
GVDFLTIGQYLQPTLKHVAVDRFVTPDEFKDYADIARGKGFLMVASSPLTRSSHHADRDFEDLRKARQDAAATK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=295, Percent_Identity=42.7118644067797, Blast_Score=255, Evalue=3e-68,
Organism=Homo sapiens, GI37577164, Length=256, Percent_Identity=43.359375, Blast_Score=223, Evalue=1e-58,
Organism=Escherichia coli, GI1786846, Length=277, Percent_Identity=53.4296028880866, Blast_Score=304, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI32564533, Length=299, Percent_Identity=40.4682274247492, Blast_Score=236, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=309, Percent_Identity=41.747572815534, Blast_Score=242, Evalue=6e-65,
Organism=Drosophila melanogaster, GI221513272, Length=299, Percent_Identity=44.4816053511706, Blast_Score=262, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_RHORT (Q2RT68)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426964.1
- ProteinModelPortal:   Q2RT68
- STRING:   Q2RT68
- GeneID:   3835301
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1877
- NMPDR:   fig|1085.1.peg.2450
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q2RT68
- ProtClustDB:   PRK05481
- BioCyc:   RRUB269796:RRU_A1877-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35104; Mature: 35104

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDTPIIRHPEKVRRPDNPSPRKPEWIRVRAPVSHEAAEVRQLMRSKNLFTVCEEAACPN
CCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCC
IGECWKRRHATFMILGDICTRACAFCNVRTGKPGHVDDQEPVNLADSVVAMGLKHVVITS
HHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEE
VDRDDLADGGAGHFHRCITEVRSRAPSCSIEVLTPDFRDKPQGALARVVEAGPDVFNHNL
CCHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHCCCH
ETVPRLYPTIRPGARYFHSLKLLDRVKTIDPGVFTKSGIMVGLGETREEVLQVMDDMRSA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCHHHHHHHHHHHHHC
GVDFLTIGQYLQPTLKHVAVDRFVTPDEFKDYADIARGKGFLMVASSPLTRSSHHADRDF
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCH
EDLRKARQDAAATK
HHHHHHHHHHCCCC
>Mature Secondary Structure
MMDTPIIRHPEKVRRPDNPSPRKPEWIRVRAPVSHEAAEVRQLMRSKNLFTVCEEAACPN
CCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCC
IGECWKRRHATFMILGDICTRACAFCNVRTGKPGHVDDQEPVNLADSVVAMGLKHVVITS
HHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEE
VDRDDLADGGAGHFHRCITEVRSRAPSCSIEVLTPDFRDKPQGALARVVEAGPDVFNHNL
CCHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHCCCH
ETVPRLYPTIRPGARYFHSLKLLDRVKTIDPGVFTKSGIMVGLGETREEVLQVMDDMRSA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCHHHHHHHHHHHHHC
GVDFLTIGQYLQPTLKHVAVDRFVTPDEFKDYADIARGKGFLMVASSPLTRSSHHADRDF
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCH
EDLRKARQDAAATK
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA