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The map label for this gene is yomI [H]

Identifier: 83593201

GI number: 83593201

Start: 2162763

End: 2164757

Strand: Direct

Name: yomI [H]

Synonym: Rru_A1866

Alternate gene names: 83593201

Gene position: 2162763-2164757 (Clockwise)

Preceding gene: 83593197

Following gene: 83593204

Centisome position: 49.69

GC content: 69.02

Gene sequence:

>1995_bases
GTGCGTTTGCTTTCCATCGTGCTCGTCTGCTTGATCATTCTGCCCCTCGCCGCCCGGGCCGCCCCGCTGTCGGTCAGCGA
TGAGGCGACCTATGCCGGCGCCTTTCGCGCCGCGGATCTCGGGCAATGGCCCCAGGCGATGGCCCTGGCGCGCAGCGCGT
CGGATCAGACGCTGCGCGATGTCGTGCTCTGGCGCCAATTGCGCGCCGCCGGCCGCGACAACGCCGGCCTTGAGGACCGC
ATGGCTTTCGTCCTCGGCCATCCCGACTGGCCCTTGCTGTCAACCATCCAGCGCCTAGCCGAAGACGACATGCTGTCGCT
GCCGCGCGCGCCCTCGCAGGTCATCGGCTTTCTCGAACGCCGCAAGCCGGTCTCCCCCGGCGGCGAGATCGCCCTGGCCC
GGGCCTATCTGGCCTCGGGCGCCCGCGACAAGGCGGCGCGGCTGATCCGCTCGGCCTGGGTCGCGGGGGATTTCACCGCC
AGCCAGGAAAAGGACCTGCTCGGCACCTATGGCGACCTGCTGCGCACCGACGACCATTGGGCGCGCGCCGACAGGGTGAT
GTGGGACAACCGCATCGACGCGGCCCGGCGCATGGTGCCGCGGCTGGACAAGGCGCGCGCCGCGGTGATCACCGCCCGGG
TGACCCTGGCCGAGGGCAAGATCGACCCCAACGCCGCGGTCAACGCCGTGCCCAAGGCCCTGCAAAATGATCCGGGTCTG
GTTTTCGAACGCGCCCGCTGGCGACGGCTGGCCGGGCTTGACGACGCGGCGATCGAGTTGATGCTCCACCGGCCCTCGGC
CGAGGGCCGGGCCGACCGCTGGTGGCAGGAACGCGCCGTTCTGCTGCGCGAGGCCTTGGATCGCGGCCTGATCACCAAGG
CCTATCGTCTGGCCGCCACCCACGAAAACGACCGGGGCGCCGCCTTCGCCGAGGGGGAATGGTTGGCCGGCTGGATCGCC
TTGCGCTTCCTTCACGACGCGCCCCAGGCCTTCAAGCACTTCAAAACCCTGTATGAAGGGGTCTCGACGCCGATCAGCCT
GTCGCGCGGCGCCTATTGGGCCGGGCGGGCGGCCGAGGCGATGAAGCGGCCCGACGAGGCGCGCCAGTGGTACCAGAAGG
CCAGCGCCTTTCCCGCCACCTTTTATGGTCAATTGGCCGCCGAGCGCACCGGCGCTCCGCTCTCGAAACTGTTGCCCAAG
GCCGATGCGGCGGCGAGCGACGCCGAACGCAAGGCCCTAGCCAATGATCGGCTGGTCGTCATCGCCAGCCAACTGGCCCA
AATCGGCCGAATCGATGAATTGCTGTCTTTCACCCTGGCTTTGAACACCAAGCACAAGGGGGACGGCGCCCGGGCGATGA
TCGCCGGCATCGCCCTGAAGGCCGGCTATCCCGGCGTCGGCGTCTTCGTGTCGCGCCGCGCCGCCCAGGATGGCACCTAT
CTGGTCGAGGCCGGCTATCCGCTGCCCAAGGCCCTAGCCCCTTCGATCAGCGAGCACGCCCGCCGCCAGGGCGTCGATCC
GGCGGCGCTTCTCGGGCTGATCCGCCAGGAAAGCAATTTCGACCGCGCGGCGCGCTCGGCGGTGGGCGCCTTGGGGCTGA
TGCAGCTTATGCCGGGCACGGCGCGCACGGTTTCAGGCCAGGAGGGGCTGGCCTTCGATCAGGCGCTGCTGACCCGCGAT
CCCCTCTACAACGTGCGGCTGGGAGCGCGCTATTTCGGCGATCTGCTCAAGCGCTTTGATGGATCGGTGGTGCTGGCGGC
GGCGGCCTATAACGCCGGCCCGGGGCGGCCGACCGAATGGATCGCCCGCAATGGCGATCCGCGCGCCATGGATCTGGCCG
ACGCCATCGACTGGATCGAGATGATCCCCTATCGCGAAACCCGCAATTACGTTCAGCGGGTGATCGAAGGCATGCTGGTT
TATCAGGTGCGCCTTGGTCAGACCCTGCCGGCCAAGGCGCCGGCTCAGGCCCTGGGCATCGCCGGGCGCTCGTAA

Upstream 100 bases:

>100_bases
GGTCCGGGCCGGATTGACGAGAGGGGAGCGGTGACGCCGCGCCAAAACCGCGCCCGCCTTTCCCCTCCAGGCTCTGACCC
CACCGCGAGGTTACCTTCCC

Downstream 100 bases:

>100_bases
GGGCACGCCGCAGACGGGCCCTGGCGCGACGGTCCTGAAATTTGCTTCAGGCACGCCGCTTTACGGGGAGAGGCGAGCGA
ATTCGTCGTCGCTTTCCGGT

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MRLLSIVLVCLIILPLAARAAPLSVSDEATYAGAFRAADLGQWPQAMALARSASDQTLRDVVLWRQLRAAGRDNAGLEDR
MAFVLGHPDWPLLSTIQRLAEDDMLSLPRAPSQVIGFLERRKPVSPGGEIALARAYLASGARDKAARLIRSAWVAGDFTA
SQEKDLLGTYGDLLRTDDHWARADRVMWDNRIDAARRMVPRLDKARAAVITARVTLAEGKIDPNAAVNAVPKALQNDPGL
VFERARWRRLAGLDDAAIELMLHRPSAEGRADRWWQERAVLLREALDRGLITKAYRLAATHENDRGAAFAEGEWLAGWIA
LRFLHDAPQAFKHFKTLYEGVSTPISLSRGAYWAGRAAEAMKRPDEARQWYQKASAFPATFYGQLAAERTGAPLSKLLPK
ADAAASDAERKALANDRLVVIASQLAQIGRIDELLSFTLALNTKHKGDGARAMIAGIALKAGYPGVGVFVSRRAAQDGTY
LVEAGYPLPKALAPSISEHARRQGVDPAALLGLIRQESNFDRAARSAVGALGLMQLMPGTARTVSGQEGLAFDQALLTRD
PLYNVRLGARYFGDLLKRFDGSVVLAAAAYNAGPGRPTEWIARNGDPRAMDLADAIDWIEMIPYRETRNYVQRVIEGMLV
YQVRLGQTLPAKAPAQALGIAGRS

Sequences:

>Translated_664_residues
MRLLSIVLVCLIILPLAARAAPLSVSDEATYAGAFRAADLGQWPQAMALARSASDQTLRDVVLWRQLRAAGRDNAGLEDR
MAFVLGHPDWPLLSTIQRLAEDDMLSLPRAPSQVIGFLERRKPVSPGGEIALARAYLASGARDKAARLIRSAWVAGDFTA
SQEKDLLGTYGDLLRTDDHWARADRVMWDNRIDAARRMVPRLDKARAAVITARVTLAEGKIDPNAAVNAVPKALQNDPGL
VFERARWRRLAGLDDAAIELMLHRPSAEGRADRWWQERAVLLREALDRGLITKAYRLAATHENDRGAAFAEGEWLAGWIA
LRFLHDAPQAFKHFKTLYEGVSTPISLSRGAYWAGRAAEAMKRPDEARQWYQKASAFPATFYGQLAAERTGAPLSKLLPK
ADAAASDAERKALANDRLVVIASQLAQIGRIDELLSFTLALNTKHKGDGARAMIAGIALKAGYPGVGVFVSRRAAQDGTY
LVEAGYPLPKALAPSISEHARRQGVDPAALLGLIRQESNFDRAARSAVGALGLMQLMPGTARTVSGQEGLAFDQALLTRD
PLYNVRLGARYFGDLLKRFDGSVVLAAAAYNAGPGRPTEWIARNGDPRAMDLADAIDWIEMIPYRETRNYVQRVIEGMLV
YQVRLGQTLPAKAPAQALGIAGRS
>Mature_664_residues
MRLLSIVLVCLIILPLAARAAPLSVSDEATYAGAFRAADLGQWPQAMALARSASDQTLRDVVLWRQLRAAGRDNAGLEDR
MAFVLGHPDWPLLSTIQRLAEDDMLSLPRAPSQVIGFLERRKPVSPGGEIALARAYLASGARDKAARLIRSAWVAGDFTA
SQEKDLLGTYGDLLRTDDHWARADRVMWDNRIDAARRMVPRLDKARAAVITARVTLAEGKIDPNAAVNAVPKALQNDPGL
VFERARWRRLAGLDDAAIELMLHRPSAEGRADRWWQERAVLLREALDRGLITKAYRLAATHENDRGAAFAEGEWLAGWIA
LRFLHDAPQAFKHFKTLYEGVSTPISLSRGAYWAGRAAEAMKRPDEARQWYQKASAFPATFYGQLAAERTGAPLSKLLPK
ADAAASDAERKALANDRLVVIASQLAQIGRIDELLSFTLALNTKHKGDGARAMIAGIALKAGYPGVGVFVSRRAAQDGTY
LVEAGYPLPKALAPSISEHARRQGVDPAALLGLIRQESNFDRAARSAVGALGLMQLMPGTARTVSGQEGLAFDQALLTRD
PLYNVRLGARYFGDLLKRFDGSVVLAAAAYNAGPGRPTEWIARNGDPRAMDLADAIDWIEMIPYRETRNYVQRVIEGMLV
YQVRLGQTLPAKAPAQALGIAGRS

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 10 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=275, Percent_Identity=26.5454545454545, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR008258
- InterPro:   IPR016047
- InterPro:   IPR010090
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01551 Peptidase_M23; PF10145 PhageMin_Tail; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 72451; Mature: 72451

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS01307 MOTA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLSIVLVCLIILPLAARAAPLSVSDEATYAGAFRAADLGQWPQAMALARSASDQTLRD
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHCCCCCCHHHHHHHHCCCHHHHHH
VVLWRQLRAAGRDNAGLEDRMAFVLGHPDWPLLSTIQRLAEDDMLSLPRAPSQVIGFLER
HHHHHHHHHCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHH
RKPVSPGGEIALARAYLASGARDKAARLIRSAWVAGDFTASQEKDLLGTYGDLLRTDDHW
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHH
ARADRVMWDNRIDAARRMVPRLDKARAAVITARVTLAEGKIDPNAAVNAVPKALQNDPGL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHCCCCCH
VFERARWRRLAGLDDAAIELMLHRPSAEGRADRWWQERAVLLREALDRGLITKAYRLAAT
HHHHHHHHHHCCCCHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
HENDRGAAFAEGEWLAGWIALRFLHDAPQAFKHFKTLYEGVSTPISLSRGAYWAGRAAEA
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
MKRPDEARQWYQKASAFPATFYGQLAAERTGAPLSKLLPKADAAASDAERKALANDRLVV
HHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHCCCCEEE
IASQLAQIGRIDELLSFTLALNTKHKGDGARAMIAGIALKAGYPGVGVFVSRRAAQDGTY
EHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCE
LVEAGYPLPKALAPSISEHARRQGVDPAALLGLIRQESNFDRAARSAVGALGLMQLMPGT
EEECCCCCCHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
ARTVSGQEGLAFDQALLTRDPLYNVRLGARYFGDLLKRFDGSVVLAAAAYNAGPGRPTEW
CCCCCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHH
IARNGDPRAMDLADAIDWIEMIPYRETRNYVQRVIEGMLVYQVRLGQTLPAKAPAQALGI
HHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCC
AGRS
CCCC
>Mature Secondary Structure
MRLLSIVLVCLIILPLAARAAPLSVSDEATYAGAFRAADLGQWPQAMALARSASDQTLRD
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHCCCCCCHHHHHHHHCCCHHHHHH
VVLWRQLRAAGRDNAGLEDRMAFVLGHPDWPLLSTIQRLAEDDMLSLPRAPSQVIGFLER
HHHHHHHHHCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHH
RKPVSPGGEIALARAYLASGARDKAARLIRSAWVAGDFTASQEKDLLGTYGDLLRTDDHW
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHH
ARADRVMWDNRIDAARRMVPRLDKARAAVITARVTLAEGKIDPNAAVNAVPKALQNDPGL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHCCCCCH
VFERARWRRLAGLDDAAIELMLHRPSAEGRADRWWQERAVLLREALDRGLITKAYRLAAT
HHHHHHHHHHCCCCHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
HENDRGAAFAEGEWLAGWIALRFLHDAPQAFKHFKTLYEGVSTPISLSRGAYWAGRAAEA
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
MKRPDEARQWYQKASAFPATFYGQLAAERTGAPLSKLLPKADAAASDAERKALANDRLVV
HHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHCCCCEEE
IASQLAQIGRIDELLSFTLALNTKHKGDGARAMIAGIALKAGYPGVGVFVSRRAAQDGTY
EHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCE
LVEAGYPLPKALAPSISEHARRQGVDPAALLGLIRQESNFDRAARSAVGALGLMQLMPGT
EEECCCCCCHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
ARTVSGQEGLAFDQALLTRDPLYNVRLGARYFGDLLKRFDGSVVLAAAAYNAGPGRPTEW
CCCCCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHH
IARNGDPRAMDLADAIDWIEMIPYRETRNYVQRVIEGMLVYQVRLGQTLPAKAPAQALGI
HHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCC
AGRS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]