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The map label for this gene is metE [H]

Identifier: 83593053

GI number: 83593053

Start: 2009449

End: 2011794

Strand: Direct

Name: metE [H]

Synonym: Rru_A1718

Alternate gene names: 83593053

Gene position: 2009449-2011794 (Clockwise)

Preceding gene: 83593049

Following gene: 83593054

Centisome position: 46.16

GC content: 64.28

Gene sequence:

>2346_bases
GTGTCTCTTCTCTCTTTGCCTATCAGCACTTTAGGGGTGCCGCGGATCGGTCCGCGTCGCGAACTCAAAACCGCGCTCGA
AGCCTATTGGTCGGGTCAATCCGACGCCAAGGCGCTTCTGGAAACCGCGGCCGCCTTGCGCGTGGCCAATTGGGCCCGCC
AGCACGCCCAGGGCGTCAGCGTCATTCCCTCGAATGATTTCTCGCTTTATGACCATGTGCTTGATACCAGCGTCATGGTC
GGCGCCATTCCCCGAGCCTATGGCTGGACGGGCGGACCCGTGCCGCTTGATACCTATTTCGCCATGGCGCGGGGCAGCCA
GGGCGCGGCCTGCGCCCATGGCGCGGCGGAAGACACCCACGGCGTTCCGGCCCTTGAAATGACCAAGTGGTTTGACACGA
ATTACCACTATATGGTTCCGGAATTCACCAAAGACCAAACCTTCACCCTGGCCTCGCGCAAGCCAGTCGAAGAATTCAAC
GAAGCCAAGGCCCTTGGCTATAAAACCCGTCCGGTTCTTCTCGGGCCGGTCACCTTCCTAAAGCTTGGAAAAAGCAAGGA
CCTCTCCCTTAATCCGCTTTCCTTGCTGGACAGCCTGCTTCCCGTATATGGTGACATTCTGCGCCAGCTTCACGAAGCGG
GCGCTGAGTGGGTGCAGATCGACGAGCCCTGCCTTGTCCTTGATCTCGACGAGCCGACGCGCGAGGCGCTGCGCCAAACT
TATTGCCGCTTCGCCGATGCCCTGCCGGGCCTGAAGATCATGCTGACCTCCTATTTCGGCGGGCTGGGCGATAATCTCGA
GACCGCCCTGACCCTGCCGGTCGCCGGCCTTCATCTCGACCTTGTCCGCGCCCCCGACCAACTCGACGCCGTGATCGCCA
AGGCGCCGCGCCGGCTGGTCTTGTCCCTTGGCGTCATCAACGGCCGTAACGTTTGGCGGGCGCCCTTGTCGGGGATCGTG
ACGTGGCTGGAACCGGTGCTGGCCCGCATCGGCGCCGAGCGGATCGAACTGGCGCCGTCGTGCTCCTTGCTGCATGTGCC
GATCGATCTCGAACTGGAAACCGATCTCGCCCCCGACCTGAAGGACTGGCTGGCCTTTTCGGTGCAGAAAATGGCCGAAC
TGGCCGTGCTCGGCCAGGCCTTGGCGCGGGGCCGGGCCTGTGTGAGCGACCAGCTCGCGGCCTCCGATCTTGCCGCCAAG
CGCCGCGCCACATCGACCAGCGTCCATGACAAGGCGGTTGCCGCCCGCCTTGCGGCGATCACCCCCGAGAGCGCCAAGCG
CTCCCACGCCTTTGCCCAGCGCGCGCCGGCGCAACGCGCTGCCCTTGGCCTGCCGCCCTTCCCGACGACGACGATCGGCT
CCTTTCCCCAAACCGCCGCGGTGCGCCAAGCCCGTTCGGCCCATGCCAAGGGCACGCTCAGCGACGCCGATTACGAGGCC
TTCCTGCGCCAAGAGACCGAGGCGGCCATTCGCTGGCAGGAGGACATCGGGCTCGATGTGCTGGTCCACGGCGAATTCGA
ACGCAACGACATGGTGCAGTATTTTGGCGAACGGCTTGCGGGCTTCGCCTTCACCCGCCACGGCTGGGTGCAAAGCTATG
GCTCGCGCTATGTGCGCCCGCCGATTTTGTTTGGCGATGTGTCGCGGCCCCAGCCGATGACGGTGTCATGGTGGCGTTAC
GCCCAATCGCTCACCGCCAAGCCGGTCAAGGGCATGGTGACCGGCCCGGTGACCATCCTCACCTGGTCCTTCGTGCGTGA
TGATGTGCCGCGCGCCGTGGCCTGCCGCCAGATCGCCCTGGCCATCGGCGACGAGGTACGCGATCTGGAAGCCGCCGGCG
CCCAGATGATCCAGATCGACGAAGCGGCGCTGCGCGAAGGATTGCCGCTGCGCAAGGCCGAATGGGGGGCGTATCTTGAC
TGGGCCATCGGTTGCTTCCGGCTTTGCGCCTCGGGCCTTGCCGATGTGACCCAGATCCACACCCATATGTGCTATTCCGA
ATTCAACGACATCATCGCCGCCATCGGCGCGATGGACGCCGATGTCATTTCCATCGAGACCTCGCGCTCGAAGATGGAGC
TGCTCGACGCCTTTCGCCTCTATAAATACCCCAATGAGATCGGTCCGGGCGTTTACGACATCCACTCGCCCCGCGTGCCC
GAGGTTGGGGAAATGGTTGATTTGCTCAGGCTCGCCCGCCAGCGCCTCGCCGATGATCAGATCTGGGTCAATCCCGATTG
CGGGCTGAAGACCCGCCACTGGGACGAGGTGCGGCCGGCGCTGGTCAATATGGTGGCGGCGGCCCGCGCCCTGCGGGCGG
TGCTGCTGACGGGAGAGGATCAATGA

Upstream 100 bases:

>100_bases
GTGTCCCGGTGGTGCGCCTGCGATCCACGGGGTTTGCCACCGTCCGGATTCCGCCGGCCTCCGCTCGGCCTTTGCCCCCA
ACGTCGATTGGGGTAAGCCG

Downstream 100 bases:

>100_bases
CCGACCGATCCGCGGGGACGGCGGCCGCGACCCGGCTGGTCGATATCGTCTTCCCCGAGCACACCAACCACCATGGCACG
CTGTTTGGCGGCATCGGCCT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 781; Mature: 780

Protein sequence:

>781_residues
MSLLSLPISTLGVPRIGPRRELKTALEAYWSGQSDAKALLETAAALRVANWARQHAQGVSVIPSNDFSLYDHVLDTSVMV
GAIPRAYGWTGGPVPLDTYFAMARGSQGAACAHGAAEDTHGVPALEMTKWFDTNYHYMVPEFTKDQTFTLASRKPVEEFN
EAKALGYKTRPVLLGPVTFLKLGKSKDLSLNPLSLLDSLLPVYGDILRQLHEAGAEWVQIDEPCLVLDLDEPTREALRQT
YCRFADALPGLKIMLTSYFGGLGDNLETALTLPVAGLHLDLVRAPDQLDAVIAKAPRRLVLSLGVINGRNVWRAPLSGIV
TWLEPVLARIGAERIELAPSCSLLHVPIDLELETDLAPDLKDWLAFSVQKMAELAVLGQALARGRACVSDQLAASDLAAK
RRATSTSVHDKAVAARLAAITPESAKRSHAFAQRAPAQRAALGLPPFPTTTIGSFPQTAAVRQARSAHAKGTLSDADYEA
FLRQETEAAIRWQEDIGLDVLVHGEFERNDMVQYFGERLAGFAFTRHGWVQSYGSRYVRPPILFGDVSRPQPMTVSWWRY
AQSLTAKPVKGMVTGPVTILTWSFVRDDVPRAVACRQIALAIGDEVRDLEAAGAQMIQIDEAALREGLPLRKAEWGAYLD
WAIGCFRLCASGLADVTQIHTHMCYSEFNDIIAAIGAMDADVISIETSRSKMELLDAFRLYKYPNEIGPGVYDIHSPRVP
EVGEMVDLLRLARQRLADDQIWVNPDCGLKTRHWDEVRPALVNMVAAARALRAVLLTGEDQ

Sequences:

>Translated_781_residues
MSLLSLPISTLGVPRIGPRRELKTALEAYWSGQSDAKALLETAAALRVANWARQHAQGVSVIPSNDFSLYDHVLDTSVMV
GAIPRAYGWTGGPVPLDTYFAMARGSQGAACAHGAAEDTHGVPALEMTKWFDTNYHYMVPEFTKDQTFTLASRKPVEEFN
EAKALGYKTRPVLLGPVTFLKLGKSKDLSLNPLSLLDSLLPVYGDILRQLHEAGAEWVQIDEPCLVLDLDEPTREALRQT
YCRFADALPGLKIMLTSYFGGLGDNLETALTLPVAGLHLDLVRAPDQLDAVIAKAPRRLVLSLGVINGRNVWRAPLSGIV
TWLEPVLARIGAERIELAPSCSLLHVPIDLELETDLAPDLKDWLAFSVQKMAELAVLGQALARGRACVSDQLAASDLAAK
RRATSTSVHDKAVAARLAAITPESAKRSHAFAQRAPAQRAALGLPPFPTTTIGSFPQTAAVRQARSAHAKGTLSDADYEA
FLRQETEAAIRWQEDIGLDVLVHGEFERNDMVQYFGERLAGFAFTRHGWVQSYGSRYVRPPILFGDVSRPQPMTVSWWRY
AQSLTAKPVKGMVTGPVTILTWSFVRDDVPRAVACRQIALAIGDEVRDLEAAGAQMIQIDEAALREGLPLRKAEWGAYLD
WAIGCFRLCASGLADVTQIHTHMCYSEFNDIIAAIGAMDADVISIETSRSKMELLDAFRLYKYPNEIGPGVYDIHSPRVP
EVGEMVDLLRLARQRLADDQIWVNPDCGLKTRHWDEVRPALVNMVAAARALRAVLLTGEDQ
>Mature_780_residues
SLLSLPISTLGVPRIGPRRELKTALEAYWSGQSDAKALLETAAALRVANWARQHAQGVSVIPSNDFSLYDHVLDTSVMVG
AIPRAYGWTGGPVPLDTYFAMARGSQGAACAHGAAEDTHGVPALEMTKWFDTNYHYMVPEFTKDQTFTLASRKPVEEFNE
AKALGYKTRPVLLGPVTFLKLGKSKDLSLNPLSLLDSLLPVYGDILRQLHEAGAEWVQIDEPCLVLDLDEPTREALRQTY
CRFADALPGLKIMLTSYFGGLGDNLETALTLPVAGLHLDLVRAPDQLDAVIAKAPRRLVLSLGVINGRNVWRAPLSGIVT
WLEPVLARIGAERIELAPSCSLLHVPIDLELETDLAPDLKDWLAFSVQKMAELAVLGQALARGRACVSDQLAASDLAAKR
RATSTSVHDKAVAARLAAITPESAKRSHAFAQRAPAQRAALGLPPFPTTTIGSFPQTAAVRQARSAHAKGTLSDADYEAF
LRQETEAAIRWQEDIGLDVLVHGEFERNDMVQYFGERLAGFAFTRHGWVQSYGSRYVRPPILFGDVSRPQPMTVSWWRYA
QSLTAKPVKGMVTGPVTILTWSFVRDDVPRAVACRQIALAIGDEVRDLEAAGAQMIQIDEAALREGLPLRKAEWGAYLDW
AIGCFRLCASGLADVTQIHTHMCYSEFNDIIAAIGAMDADVISIETSRSKMELLDAFRLYKYPNEIGPGVYDIHSPRVPE
VGEMVDLLRLARQRLADDQIWVNPDCGLKTRHWDEVRPALVNMVAAARALRAVLLTGEDQ

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=773, Percent_Identity=53.0401034928849, Blast_Score=779, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=768, Percent_Identity=48.9583333333333, Blast_Score=728, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 85693; Mature: 85562

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLSLPISTLGVPRIGPRRELKTALEAYWSGQSDAKALLETAAALRVANWARQHAQGVS
CCCHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
VIPSNDFSLYDHVLDTSVMVGAIPRAYGWTGGPVPLDTYFAMARGSQGAACAHGAAEDTH
EECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
GVPALEMTKWFDTNYHYMVPEFTKDQTFTLASRKPVEEFNEAKALGYKTRPVLLGPVTFL
CCCHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCHHHH
KLGKSKDLSLNPLSLLDSLLPVYGDILRQLHEAGAEWVQIDEPCLVLDLDEPTREALRQT
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHH
YCRFADALPGLKIMLTSYFGGLGDNLETALTLPVAGLHLDLVRAPDQLDAVIAKAPRRLV
HHHHHHCCCCHHHHHHHHHCCCCCCCHHEEECCCCCCEEEECCCCHHHHHHHHHCCHHHH
LSLGVINGRNVWRAPLSGIVTWLEPVLARIGAERIELAPSCSLLHVPIDLELETDLAPDL
HHEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHEEECCCCCEEEEEEEEEECCCCCCCH
KDWLAFSVQKMAELAVLGQALARGRACVSDQLAASDLAAKRRATSTSVHDKAVAARLAAI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
TPESAKRSHAFAQRAPAQRAALGLPPFPTTTIGSFPQTAAVRQARSAHAKGTLSDADYEA
CCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
FLRQETEAAIRWQEDIGLDVLVHGEFERNDMVQYFGERLAGFAFTRHGWVQSYGSRYVRP
HHHHHHHHHHEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PILFGDVSRPQPMTVSWWRYAQSLTAKPVKGMVTGPVTILTWSFVRDDVPRAVACRQIAL
CEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHEEHHHHHHCCHHHHHHHHHHH
AIGDEVRDLEAAGAQMIQIDEAALREGLPLRKAEWGAYLDWAIGCFRLCASGLADVTQIH
HHCCHHHHHHHCCCCEEEECHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
THMCYSEFNDIIAAIGAMDADVISIETSRSKMELLDAFRLYKYPNEIGPGVYDIHSPRVP
HHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHCCCEEECCCCCCC
EVGEMVDLLRLARQRLADDQIWVNPDCGLKTRHWDEVRPALVNMVAAARALRAVLLTGED
CHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
Q
C
>Mature Secondary Structure 
SLLSLPISTLGVPRIGPRRELKTALEAYWSGQSDAKALLETAAALRVANWARQHAQGVS
CCHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
VIPSNDFSLYDHVLDTSVMVGAIPRAYGWTGGPVPLDTYFAMARGSQGAACAHGAAEDTH
EECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
GVPALEMTKWFDTNYHYMVPEFTKDQTFTLASRKPVEEFNEAKALGYKTRPVLLGPVTFL
CCCHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCHHHH
KLGKSKDLSLNPLSLLDSLLPVYGDILRQLHEAGAEWVQIDEPCLVLDLDEPTREALRQT
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHH
YCRFADALPGLKIMLTSYFGGLGDNLETALTLPVAGLHLDLVRAPDQLDAVIAKAPRRLV
HHHHHHCCCCHHHHHHHHHCCCCCCCHHEEECCCCCCEEEECCCCHHHHHHHHHCCHHHH
LSLGVINGRNVWRAPLSGIVTWLEPVLARIGAERIELAPSCSLLHVPIDLELETDLAPDL
HHEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHEEECCCCCEEEEEEEEEECCCCCCCH
KDWLAFSVQKMAELAVLGQALARGRACVSDQLAASDLAAKRRATSTSVHDKAVAARLAAI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
TPESAKRSHAFAQRAPAQRAALGLPPFPTTTIGSFPQTAAVRQARSAHAKGTLSDADYEA
CCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
FLRQETEAAIRWQEDIGLDVLVHGEFERNDMVQYFGERLAGFAFTRHGWVQSYGSRYVRP
HHHHHHHHHHEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PILFGDVSRPQPMTVSWWRYAQSLTAKPVKGMVTGPVTILTWSFVRDDVPRAVACRQIAL
CEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHEEHHHHHHCCHHHHHHHHHHH
AIGDEVRDLEAAGAQMIQIDEAALREGLPLRKAEWGAYLDWAIGCFRLCASGLADVTQIH
HHCCHHHHHHHCCCCEEEECHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
THMCYSEFNDIIAAIGAMDADVISIETSRSKMELLDAFRLYKYPNEIGPGVYDIHSPRVP
HHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHCCCEEECCCCCCC
EVGEMVDLLRLARQRLADDQIWVNPDCGLKTRHWDEVRPALVNMVAAARALRAVLLTGED
CHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA