Click here to switch to the map view.

The map label for this gene is dusA [H]

Identifier: 83592956

GI number: 83592956

Start: 1913328

End: 1914377

Strand: Reverse

Name: dusA [H]

Synonym: Rru_A1621

Alternate gene names: 83592956

Gene position: 1914377-1913328 (Counterclockwise)

Preceding gene: 83592958

Following gene: 83592953

Centisome position: 43.98

GC content: 67.43

Gene sequence:

>1050_bases
GTGATGGTCTCCGCGCCCGCTCCCCGATCCCAGCCCGACCGATCCCAGCCCGACCGCCGGCTGTCCGTCGCCCCGATGAT
GGACTGGACCGACCGTCATGATCGCTGGTTCTTGCGCCAGATCACCAAGCGCACCTTGCTCTATACGGAAATGATCACGG
CCCAGGCCCTGTTGCACGCCGATCCCGGGCGCTTTCTGGACCATCACCCCGACGAGCATCCTTTGGCCCTGCAGCTTGGC
GGCAGCGATCCCCGGGCCCTGGCCGAGGCCACGCGCCTAGCCGCGCCCTTTGGCTTCGCCGAGATCAACCTCAACGTCGG
CTGTCCGTCCGATCGGGTGAGCAGCGGCCGCTTCGGCGCCTGCCTGATGGCCGATCCCGCCCTGGTCGCCGAGTGCCTGT
CGGCGATGGCCGAGGCGTCAAACGCGCCGGTAACGATCAAACATCGCATCGGCATCGATGATCTCGACAGCTATGACCAG
CTTGTCGGCTTTGTCGATCAGGTGGCGCGAAGCGGCATCGCCACGATCATCGTCCATGCCCGCAAAGCGTGGCTAAAGGG
CCTCAGCCCCAAGGAAAACCGCGAGATTCCGCCCTTGCGCCACGATGTCGTCCACCGGCTGAAGGCGGATTTTCCCGGGC
TGGAGGTTATTCTCAACGGCGGCATCACCACGCTGACCCAGGCGCTTGACCATCTGGGGTCAGGGGACCCTGCGGCGCCG
GCGGTCGATGGGGTGATGATCGGCCGCGCCGCCTATGAGACGCCCTATATGCTGGCCCAGGCCGATGGCCTGATCTTTGG
CGAGGCCACGCCCGCGCCCTCGCGCCACGAGGTTGCCCTGGCCTTGCTGCCCTATCTAGACGCCATGACCGCGCGCGGCG
AACCGGTGAAGCGGCTGACCCGCCATATCCTGGGATTGTTCCACGGGCAACCGGGGGCCCGGGCCTATCGCCGCCACCTG
GGCGAGGCCGGGGTCCGCCCCGATGCCACGGCCCAGGTGCTGATCGACGCCCTGGCCCATATCCCCGCCCTTGACGAGGC
CGAGAGCTGA

Upstream 100 bases:

>100_bases
TTCCGATCCATCGGCTGCGCCATCAGACCAAAGGTGGACCCCGGGACTCCTCCCCCACACGGCCGTCGGGCCGGTTTTTT
CGCAAGGACCAAGGCTGCCC

Downstream 100 bases:

>100_bases
AGACCGGTCGGGACTTGCCCTAATCTAATACCGCATCTTGGCTTCGATGGCCCGCTGCTGGCCGGCCTCCAACCCCAGGG
CGGCGTCGGAAAAACTGGGG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MMVSAPAPRSQPDRSQPDRRLSVAPMMDWTDRHDRWFLRQITKRTLLYTEMITAQALLHADPGRFLDHHPDEHPLALQLG
GSDPRALAEATRLAAPFGFAEINLNVGCPSDRVSSGRFGACLMADPALVAECLSAMAEASNAPVTIKHRIGIDDLDSYDQ
LVGFVDQVARSGIATIIVHARKAWLKGLSPKENREIPPLRHDVVHRLKADFPGLEVILNGGITTLTQALDHLGSGDPAAP
AVDGVMIGRAAYETPYMLAQADGLIFGEATPAPSRHEVALALLPYLDAMTARGEPVKRLTRHILGLFHGQPGARAYRRHL
GEAGVRPDATAQVLIDALAHIPALDEAES

Sequences:

>Translated_349_residues
MMVSAPAPRSQPDRSQPDRRLSVAPMMDWTDRHDRWFLRQITKRTLLYTEMITAQALLHADPGRFLDHHPDEHPLALQLG
GSDPRALAEATRLAAPFGFAEINLNVGCPSDRVSSGRFGACLMADPALVAECLSAMAEASNAPVTIKHRIGIDDLDSYDQ
LVGFVDQVARSGIATIIVHARKAWLKGLSPKENREIPPLRHDVVHRLKADFPGLEVILNGGITTLTQALDHLGSGDPAAP
AVDGVMIGRAAYETPYMLAQADGLIFGEATPAPSRHEVALALLPYLDAMTARGEPVKRLTRHILGLFHGQPGARAYRRHL
GEAGVRPDATAQVLIDALAHIPALDEAES
>Mature_349_residues
MMVSAPAPRSQPDRSQPDRRLSVAPMMDWTDRHDRWFLRQITKRTLLYTEMITAQALLHADPGRFLDHHPDEHPLALQLG
GSDPRALAEATRLAAPFGFAEINLNVGCPSDRVSSGRFGACLMADPALVAECLSAMAEASNAPVTIKHRIGIDDLDSYDQ
LVGFVDQVARSGIATIIVHARKAWLKGLSPKENREIPPLRHDVVHRLKADFPGLEVILNGGITTLTQALDHLGSGDPAAP
AVDGVMIGRAAYETPYMLAQADGLIFGEATPAPSRHEVALALLPYLDAMTARGEPVKRLTRHILGLFHGQPGARAYRRHL
GEAGVRPDATAQVLIDALAHIPALDEAES

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=243, Percent_Identity=29.2181069958848, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI8923374, Length=195, Percent_Identity=32.8205128205128, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI145693211, Length=320, Percent_Identity=51.875, Blast_Score=324, Evalue=4e-90,
Organism=Escherichia coli, GI1789660, Length=251, Percent_Identity=27.8884462151394, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788462, Length=183, Percent_Identity=31.6939890710383, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37801; Mature: 37801

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVSAPAPRSQPDRSQPDRRLSVAPMMDWTDRHDRWFLRQITKRTLLYTEMITAQALLHA
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPGRFLDHHPDEHPLALQLGGSDPRALAEATRLAAPFGFAEINLNVGCPSDRVSSGRFGA
CCCCHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCE
CLMADPALVAECLSAMAEASNAPVTIKHRIGIDDLDSYDQLVGFVDQVARSGIATIIVHA
EEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RKAWLKGLSPKENREIPPLRHDVVHRLKADFPGLEVILNGGITTLTQALDHLGSGDPAAP
HHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCC
AVDGVMIGRAAYETPYMLAQADGLIFGEATPAPSRHEVALALLPYLDAMTARGEPVKRLT
HHHHHHCCHHHHCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
RHILGLFHGQPGARAYRRHLGEAGVRPDATAQVLIDALAHIPALDEAES
HHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMVSAPAPRSQPDRSQPDRRLSVAPMMDWTDRHDRWFLRQITKRTLLYTEMITAQALLHA
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPGRFLDHHPDEHPLALQLGGSDPRALAEATRLAAPFGFAEINLNVGCPSDRVSSGRFGA
CCCCHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCE
CLMADPALVAECLSAMAEASNAPVTIKHRIGIDDLDSYDQLVGFVDQVARSGIATIIVHA
EEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RKAWLKGLSPKENREIPPLRHDVVHRLKADFPGLEVILNGGITTLTQALDHLGSGDPAAP
HHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCC
AVDGVMIGRAAYETPYMLAQADGLIFGEATPAPSRHEVALALLPYLDAMTARGEPVKRLT
HHHHHHCCHHHHCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
RHILGLFHGQPGARAYRRHLGEAGVRPDATAQVLIDALAHIPALDEAES
HHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]