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The map label for this gene is gltX

Identifier: 83592936

GI number: 83592936

Start: 1886099

End: 1887505

Strand: Reverse

Name: gltX

Synonym: Rru_A1601

Alternate gene names: 83592936

Gene position: 1887505-1886099 (Counterclockwise)

Preceding gene: 83592938

Following gene: 83592935

Centisome position: 43.36

GC content: 66.03

Gene sequence:

>1407_bases
ATGACCGTCGTCACCCGTTTTGCGCCCTCGCCCACCGGTTTCCTGCATATCGGCGGCGCCCGTACCGCCCTGTTCAACTG
GTTGTTCGCCCGCCATCACGGTGGCCGCTTCCTGTTGCGCATCGAAGACACGGACCGCGTGCGATCCACCCCCGAGGCGG
TGGCGGCGATTTTCGATGGTTTGGAATGGCTGGGGCTGGATTGGGACGAAGAGCCGACCTTCCAGTTCGCCCGCGCCGCC
CGCCATGCCGAAGCCGCCCACGAGCTGGTGGCCAAGGGTCTGGCCTATCGCTGCTATTGCACCCCCGACGAGTTGACGGC
GATGCGCGAGGAGCAGAAAGCCAAGGGCCTGCCGCCGCGCTATAACGGCCTGTGGCGCGATCGCGATCCCTCCGAGGCCC
CCGCTGGCGTCGCCCCGGTGATCCGCCTCAAGGCGCCCCAGGACGGCGAGACGACGATCACCGATTCCGTTCAAGGGGCG
GTGACGGTCGCCAACAACCAGCTTGACGACATGATCTTGCTGCGCAGCGACGGCACGCCGACCTATATGCTCTCGGTGGT
GGTCGATGACCACGACATGGGGGTAACCCATGTCATTCGCGGCGACGATCACCTGACCAACGCCTTCCGCCAGACCCAGC
TCTACTGGGCCCTGGGATGGGAGACGCCGGTCTTCGCCCATATTCCGCTGATCCACGGCGCCGATGGCGCCAAGCTGTCC
AAGCGCCATGGCGCGCTGGGCGCCGAGGCCTATCGCGACATGGGCTTCCTGCCCGAGGCCTTGCGCAATTATCTGGTCCG
CCTGGGCTGGGCCCATGGCGACGACGAGGTGTTCACCACCGAACAGGCCGTCGAATGGTTCAGCCTGGAAAGCATCGGCC
GCTCGCCCTCGCGCTTCGATATGCAGAAGCTCACGGCGCTCAACGGGTCGTATATGCGCGAAACCGATGACGACCGCCTG
ACCGCCCTGCTCGTTCCCCGACTCGAATCCACCTTGGGCCTCGACATCCCGGCCGCCGGCCATGCCCTGTTGCGGGCGGG
AATGGCCGGGCTGAAGGAGCGGGCCAAGACCCTGGTCGAACTGGCCGACCTCGCCGCCTTCTATGTCCGGCCGCGCCCTT
TGGCGATCGACGCCAAGGCCGAAAAGGCCCTTGATGACGAGGGGCGGACGATCCTGGCCGCCCTGATCGACAAGCTTGAT
GACTTCTCCCCGTGGACGCGCGACTCCTTGGAAAATCTCGCGCGCGTAATGTCCGAGGAACGCGGGGTGAAATTGGGCAA
GGTCGCCCAGCCCATCCGCGCGGCGCTGACCGGCAGCACGGTCTCGCCGCCGATCTTCGAGGTCATGGAAATCCTTGGCC
CCGACGAGACTCTCGGGCGTCTTCGCGACGCCCAAAGCCACTCCTAA

Upstream 100 bases:

>100_bases
CGTGTGCCCCGCGCACCTTGTCTTCCCGCCTGCCCGTACCCTTTCTGGCTTTCCCATTCCTGGCTTTCCCATTCCTGGCT
TTCCCATTCCGGACACGATC

Downstream 100 bases:

>100_bases
TAGCAAGGTCCGCCGGGCAACAAAAAATTGATCGCGGTGATGGGGCTTGGTGTATTGTGCGCCACGCCGATGGGTTCGCA
GATGCCCGCCGAATTTCTAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTVVTRFAPSPTGFLHIGGARTALFNWLFARHHGGRFLLRIEDTDRVRSTPEAVAAIFDGLEWLGLDWDEEPTFQFARAA
RHAEAAHELVAKGLAYRCYCTPDELTAMREEQKAKGLPPRYNGLWRDRDPSEAPAGVAPVIRLKAPQDGETTITDSVQGA
VTVANNQLDDMILLRSDGTPTYMLSVVVDDHDMGVTHVIRGDDHLTNAFRQTQLYWALGWETPVFAHIPLIHGADGAKLS
KRHGALGAEAYRDMGFLPEALRNYLVRLGWAHGDDEVFTTEQAVEWFSLESIGRSPSRFDMQKLTALNGSYMRETDDDRL
TALLVPRLESTLGLDIPAAGHALLRAGMAGLKERAKTLVELADLAAFYVRPRPLAIDAKAEKALDDEGRTILAALIDKLD
DFSPWTRDSLENLARVMSEERGVKLGKVAQPIRAALTGSTVSPPIFEVMEILGPDETLGRLRDAQSHS

Sequences:

>Translated_468_residues
MTVVTRFAPSPTGFLHIGGARTALFNWLFARHHGGRFLLRIEDTDRVRSTPEAVAAIFDGLEWLGLDWDEEPTFQFARAA
RHAEAAHELVAKGLAYRCYCTPDELTAMREEQKAKGLPPRYNGLWRDRDPSEAPAGVAPVIRLKAPQDGETTITDSVQGA
VTVANNQLDDMILLRSDGTPTYMLSVVVDDHDMGVTHVIRGDDHLTNAFRQTQLYWALGWETPVFAHIPLIHGADGAKLS
KRHGALGAEAYRDMGFLPEALRNYLVRLGWAHGDDEVFTTEQAVEWFSLESIGRSPSRFDMQKLTALNGSYMRETDDDRL
TALLVPRLESTLGLDIPAAGHALLRAGMAGLKERAKTLVELADLAAFYVRPRPLAIDAKAEKALDDEGRTILAALIDKLD
DFSPWTRDSLENLARVMSEERGVKLGKVAQPIRAALTGSTVSPPIFEVMEILGPDETLGRLRDAQSHS
>Mature_467_residues
TVVTRFAPSPTGFLHIGGARTALFNWLFARHHGGRFLLRIEDTDRVRSTPEAVAAIFDGLEWLGLDWDEEPTFQFARAAR
HAEAAHELVAKGLAYRCYCTPDELTAMREEQKAKGLPPRYNGLWRDRDPSEAPAGVAPVIRLKAPQDGETTITDSVQGAV
TVANNQLDDMILLRSDGTPTYMLSVVVDDHDMGVTHVIRGDDHLTNAFRQTQLYWALGWETPVFAHIPLIHGADGAKLSK
RHGALGAEAYRDMGFLPEALRNYLVRLGWAHGDDEVFTTEQAVEWFSLESIGRSPSRFDMQKLTALNGSYMRETDDDRLT
ALLVPRLESTLGLDIPAAGHALLRAGMAGLKERAKTLVELADLAAFYVRPRPLAIDAKAEKALDDEGRTILAALIDKLDD
FSPWTRDSLENLARVMSEERGVKLGKVAQPIRAALTGSTVSPPIFEVMEILGPDETLGRLRDAQSHS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=496, Percent_Identity=32.4596774193548, Blast_Score=235, Evalue=7e-62,
Organism=Escherichia coli, GI1788743, Length=465, Percent_Identity=43.8709677419355, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI226510925, Length=284, Percent_Identity=37.3239436619718, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1786895, Length=131, Percent_Identity=35.1145038167939, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=483, Percent_Identity=32.2981366459627, Blast_Score=217, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6324540, Length=498, Percent_Identity=33.9357429718875, Blast_Score=243, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6321192, Length=230, Percent_Identity=28.695652173913, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=111, Percent_Identity=36.036036036036, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=35.5555555555556, Blast_Score=264, Evalue=9e-71,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_RHORT (Q2RTZ4)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426688.1
- ProteinModelPortal:   Q2RTZ4
- SMR:   Q2RTZ4
- STRING:   Q2RTZ4
- GeneID:   3835018
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1601
- NMPDR:   fig|1085.1.peg.2766
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q2RTZ4
- ProtClustDB:   PRK01406
- BioCyc:   RRUB269796:RRU_A1601-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51709; Mature: 51578

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVTRFAPSPTGFLHIGGARTALFNWLFARHHGGRFLLRIEDTDRVRSTPEAVAAIFDG
CCEEEECCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHHCC
LEWLGLDWDEEPTFQFARAARHAEAAHELVAKGLAYRCYCTPDELTAMREEQKAKGLPPR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCC
YNGLWRDRDPSEAPAGVAPVIRLKAPQDGETTITDSVQGAVTVANNQLDDMILLRSDGTP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCEEEEECCCCCCEEEEECCCCC
TYMLSVVVDDHDMGVTHVIRGDDHLTNAFRQTQLYWALGWETPVFAHIPLIHGADGAKLS
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHEEEEEECCCCCCEEEECEEECCCCCHHH
KRHGALGAEAYRDMGFLPEALRNYLVRLGWAHGDDEVFTTEQAVEWFSLESIGRSPSRFD
HHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHH
MQKLTALNGSYMRETDDDRLTALLVPRLESTLGLDIPAAGHALLRAGMAGLKERAKTLVE
HHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LADLAAFYVRPRPLAIDAKAEKALDDEGRTILAALIDKLDDFSPWTRDSLENLARVMSEE
HHHHHHHHCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RGVKLGKVAQPIRAALTGSTVSPPIFEVMEILGPDETLGRLRDAQSHS
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TVVTRFAPSPTGFLHIGGARTALFNWLFARHHGGRFLLRIEDTDRVRSTPEAVAAIFDG
CEEEECCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHHCC
LEWLGLDWDEEPTFQFARAARHAEAAHELVAKGLAYRCYCTPDELTAMREEQKAKGLPPR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCC
YNGLWRDRDPSEAPAGVAPVIRLKAPQDGETTITDSVQGAVTVANNQLDDMILLRSDGTP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCEEEEECCCCCCEEEEECCCCC
TYMLSVVVDDHDMGVTHVIRGDDHLTNAFRQTQLYWALGWETPVFAHIPLIHGADGAKLS
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHEEEEEECCCCCCEEEECEEECCCCCHHH
KRHGALGAEAYRDMGFLPEALRNYLVRLGWAHGDDEVFTTEQAVEWFSLESIGRSPSRFD
HHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHH
MQKLTALNGSYMRETDDDRLTALLVPRLESTLGLDIPAAGHALLRAGMAGLKERAKTLVE
HHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LADLAAFYVRPRPLAIDAKAEKALDDEGRTILAALIDKLDDFSPWTRDSLENLARVMSEE
HHHHHHHHCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RGVKLGKVAQPIRAALTGSTVSPPIFEVMEILGPDETLGRLRDAQSHS
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA