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The map label for this gene is ccsA [H]

Identifier: 83592935

GI number: 83592935

Start: 1884670

End: 1885971

Strand: Reverse

Name: ccsA [H]

Synonym: Rru_A1600

Alternate gene names: 83592935

Gene position: 1885971-1884670 (Counterclockwise)

Preceding gene: 83592936

Following gene: 83592909

Centisome position: 43.33

GC content: 60.14

Gene sequence:

>1302_bases
ATGACGGACTCCAAGCAAACCGTGACCCTTGTCGACAACCGCTCAGGAAAAAGTTACGAATTTCCCATCCTGAGCGGTTC
GACCGGGCCCGATGTGGTAGATATTCGCAAGTTCTACACGGAAACGGGGATGTTCACCTACGATCCGGGCTATACCTCGA
CGGGCAGCTGTTCCTCCGCCGTGACCTATATCGACGGCGACGCCGGCATCCTGCTCCACCGTGGCTATGCCATCGAAGAC
CTTGCCATGCACTGCGACTTTACCGAAGTCGCCTATCTTTTGCTGCGCGGCGAATTGCCGACGGCGCCCAAGAAGACCGA
GTTCGAGAAGATCATCACCTATCACACGATGGTCCACGAACAGCTGCACAAGTTCTATGACGGCTTCCGCCGCGATGCCC
ATCCGATGGCCGTCATGTGCGGCGTGGTCGGGGCGCTCTCGGCCTTCTATCACGACAGCATCGACATCACCGATCCGATC
CAGCGCATGATCGCCCAGCACCGGCTGGTGGCGAAGATGCCGACGATCGCCGCCTGGGCCTATAAGTACTCCCAGGGCCA
GCCCTTCATGTATCCGCGCAACAACCTGACCTACGCGGAAAACTTCCTGCACATGATGTTCGCCACCCCGTGCGAGGAAT
ACAAGGTCAGCCCGGTTCTGGCCCGCGCCATGGACCGCATCCTGATCTTGCATGCCGACCACGAGCAGAACGCCTCGACC
TCGACCGTGCGGCTGGCCGGATCGTCGGGGGCCAATCCCTTCGCCTGTATCGCCGCCGGCATCGCCTCGCTGTGGGGCCC
CGCCCATGGCGGCGCCAATGAGGCCGTGCTGAAGATGCTGACCGAGATCGGCAGCCCCGATCGCGTTGACGACTTCGTCA
AGAAAGCCAAGGACAAGAACGATCCGTTCCGTCTGATGGGCTTTGGTCACCGCGTTTACAAAAATTACGACCCGCGCGCC
AAGATCATGGCCGCGACCTGCCACGAAGTGCTCGACGAGCTTGGCATCGTCGACGAGCCGCTGCTTGAAATCGCCATGCG
GCTGGAAAAGATCGCGCTGGAAGACGAATACTTCATCGAAAAGAAGCTCTATCCCAACGTCGATTTCTATTCCGGCATCA
TCTTCCGCGCCATCGGCATCCCGACCTCGATGTTCACCTGCCTGTTCGCCCTGGCGCGCACCGTTGGCTGGATCAGCCAG
TGGAAGGAAATGATCGAGGACCCCGACCAGAAGATCGGCCGGCCGCGCCAGCTGTACAATGGCGCCACCAAACGCGACTT
CCTGCCGATGGACAAGCGCTAA

Upstream 100 bases:

>100_bases
TTGATCGCGGTGATGGGGCTTGGTGTATTGTGCGCCACGCCGATGGGTTCGCAGATGCCCGCCGAATTTCTAGAATCACT
CTAGCATGGGGGAATGACCT

Downstream 100 bases:

>100_bases
ACCCAAGGCTTTTCGCTCCCGATCAAAAAAAGGCGGCCTCCACAAAGGAGACCGCCTTTTTATCGTCCCGCTCTCCGGCG
GCTCACCGCCTTAGATCAGG

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTDSKQTVTLVDNRSGKSYEFPILSGSTGPDVVDIRKFYTETGMFTYDPGYTSTGSCSSAVTYIDGDAGILLHRGYAIED
LAMHCDFTEVAYLLLRGELPTAPKKTEFEKIITYHTMVHEQLHKFYDGFRRDAHPMAVMCGVVGALSAFYHDSIDITDPI
QRMIAQHRLVAKMPTIAAWAYKYSQGQPFMYPRNNLTYAENFLHMMFATPCEEYKVSPVLARAMDRILILHADHEQNAST
STVRLAGSSGANPFACIAAGIASLWGPAHGGANEAVLKMLTEIGSPDRVDDFVKKAKDKNDPFRLMGFGHRVYKNYDPRA
KIMAATCHEVLDELGIVDEPLLEIAMRLEKIALEDEYFIEKKLYPNVDFYSGIIFRAIGIPTSMFTCLFALARTVGWISQ
WKEMIEDPDQKIGRPRQLYNGATKRDFLPMDKR

Sequences:

>Translated_433_residues
MTDSKQTVTLVDNRSGKSYEFPILSGSTGPDVVDIRKFYTETGMFTYDPGYTSTGSCSSAVTYIDGDAGILLHRGYAIED
LAMHCDFTEVAYLLLRGELPTAPKKTEFEKIITYHTMVHEQLHKFYDGFRRDAHPMAVMCGVVGALSAFYHDSIDITDPI
QRMIAQHRLVAKMPTIAAWAYKYSQGQPFMYPRNNLTYAENFLHMMFATPCEEYKVSPVLARAMDRILILHADHEQNAST
STVRLAGSSGANPFACIAAGIASLWGPAHGGANEAVLKMLTEIGSPDRVDDFVKKAKDKNDPFRLMGFGHRVYKNYDPRA
KIMAATCHEVLDELGIVDEPLLEIAMRLEKIALEDEYFIEKKLYPNVDFYSGIIFRAIGIPTSMFTCLFALARTVGWISQ
WKEMIEDPDQKIGRPRQLYNGATKRDFLPMDKR
>Mature_432_residues
TDSKQTVTLVDNRSGKSYEFPILSGSTGPDVVDIRKFYTETGMFTYDPGYTSTGSCSSAVTYIDGDAGILLHRGYAIEDL
AMHCDFTEVAYLLLRGELPTAPKKTEFEKIITYHTMVHEQLHKFYDGFRRDAHPMAVMCGVVGALSAFYHDSIDITDPIQ
RMIAQHRLVAKMPTIAAWAYKYSQGQPFMYPRNNLTYAENFLHMMFATPCEEYKVSPVLARAMDRILILHADHEQNASTS
TVRLAGSSGANPFACIAAGIASLWGPAHGGANEAVLKMLTEIGSPDRVDDFVKKAKDKNDPFRLMGFGHRVYKNYDPRAK
IMAATCHEVLDELGIVDEPLLEIAMRLEKIALEDEYFIEKKLYPNVDFYSGIIFRAIGIPTSMFTCLFALARTVGWISQW
KEMIEDPDQKIGRPRQLYNGATKRDFLPMDKR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=349, Percent_Identity=26.9340974212034, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786939, Length=433, Percent_Identity=65.5889145496536, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI1786527, Length=371, Percent_Identity=33.6927223719677, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17555174, Length=445, Percent_Identity=25.3932584269663, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319850, Length=329, Percent_Identity=28.2674772036474, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324328, Length=343, Percent_Identity=26.2390670553936, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325257, Length=367, Percent_Identity=26.158038147139, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24640126, Length=421, Percent_Identity=26.603325415677, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24640124, Length=421, Percent_Identity=26.603325415677, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21356863, Length=353, Percent_Identity=27.4787535410765, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48745; Mature: 48614

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSKQTVTLVDNRSGKSYEFPILSGSTGPDVVDIRKFYTETGMFTYDPGYTSTGSCSSA
CCCCCCEEEEEECCCCCCEECEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCE
VTYIDGDAGILLHRGYAIEDLAMHCDFTEVAYLLLRGELPTAPKKTEFEKIITYHTMVHE
EEEEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
QLHKFYDGFRRDAHPMAVMCGVVGALSAFYHDSIDITDPIQRMIAQHRLVAKMPTIAAWA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
YKYSQGQPFMYPRNNLTYAENFLHMMFATPCEEYKVSPVLARAMDRILILHADHEQNAST
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCEEEEECCCCCCCCC
STVRLAGSSGANPFACIAAGIASLWGPAHGGANEAVLKMLTEIGSPDRVDDFVKKAKDKN
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCC
DPFRLMGFGHRVYKNYDPRAKIMAATCHEVLDELGIVDEPLLEIAMRLEKIALEDEYFIE
CCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
KKLYPNVDFYSGIIFRAIGIPTSMFTCLFALARTVGWISQWKEMIEDPDQKIGRPRQLYN
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHC
GATKRDFLPMDKR
CCCCCCCCCCCCC
>Mature Secondary Structure 
TDSKQTVTLVDNRSGKSYEFPILSGSTGPDVVDIRKFYTETGMFTYDPGYTSTGSCSSA
CCCCCEEEEEECCCCCCEECEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCE
VTYIDGDAGILLHRGYAIEDLAMHCDFTEVAYLLLRGELPTAPKKTEFEKIITYHTMVHE
EEEEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
QLHKFYDGFRRDAHPMAVMCGVVGALSAFYHDSIDITDPIQRMIAQHRLVAKMPTIAAWA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
YKYSQGQPFMYPRNNLTYAENFLHMMFATPCEEYKVSPVLARAMDRILILHADHEQNAST
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCEEEEECCCCCCCCC
STVRLAGSSGANPFACIAAGIASLWGPAHGGANEAVLKMLTEIGSPDRVDDFVKKAKDKN
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCC
DPFRLMGFGHRVYKNYDPRAKIMAATCHEVLDELGIVDEPLLEIAMRLEKIALEDEYFIE
CCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
KKLYPNVDFYSGIIFRAIGIPTSMFTCLFALARTVGWISQWKEMIEDPDQKIGRPRQLYN
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHC
GATKRDFLPMDKR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]