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The map label for this gene is metF [H]

Identifier: 83592865

GI number: 83592865

Start: 1800682

End: 1801641

Strand: Direct

Name: metF [H]

Synonym: Rru_A1530

Alternate gene names: 83592865

Gene position: 1800682-1801641 (Clockwise)

Preceding gene: 83592864

Following gene: 83592866

Centisome position: 41.37

GC content: 66.67

Gene sequence:

>960_bases
GTGACCGTGACGGCTTCGCCGCTGATCGCCCGTCCGCGCGCCCCTCGCGCCCTCTCGGTGTCGTTCGAATTCTTTCCGCC
CAAAACCGACCGCATGCGCGACCAACTGTGGGACTGCCTGCGCCAGCTCGAGCCGCTGGAGCCGTCCTTTGTGTCGGTGA
CCTATGGGGCGGGGGGATCGACGCGCGAGCGCACCCATGAGACGGTGGCGAGGATCGCCGCCGACACCACCATGAAGCCG
GCCGCCCATCTGACCTGCGTCGAGGCGACGCGGGCCGAGGTCAACGCGGTGGCGCAAGCCTATTGGCAGGCCGGCGTGCG
CCATGTTGTGGCGCTGCGTGGCGATCCGCCGACGCTTGGCGGCGCCTATGTTCCCCATCCCGGCGGCTACGCCTATGCCC
TGGATCTGGTCAAGGGCTTGCGCGACGTCGCCGATTTCGAGATTAGCGTGGCGGCCTATCCCGAGGTTCATCCCCAGGCG
CGCAGCGCCGCCGAGGATCTTGATAACCTCAAGCGCAAGATCGATGCCGGGGCGACGCGGGCGATCAGCCAGTATTTCTT
CGATGTCGACAGCTATCGCCGGTTCATGGACCGGGTGTTGGCGGCGGGAATCAGCGTGCCGGTGGTGCCCGGCATCTTGC
CGGTGGGCAATTTCCAAACGGTGCTGAAGTTCTCCAACGCCTGCGGCACCCGGGTGCCGAAATGGATGCACGAGCTGTTC
GCCGGCCTTGAGAACGACCCGGAAACCCACCGCATGGTGGCGGCGACCCTGGCGGCCGAACAGTGCCTGTCGCTGTTCGA
AGACGGGGTCGACTGTTTCCACTTTTATACCCTGAACCGTGCCGATCTGGTTCGCGCCGTCTGTCACATGCTGGGCCTGC
GCCCGGCCGCCATTCCCACCGCCTCGCGTCTCGCGCCCGATCAGGGCGCGGGGACCATCGCCCTTGGAGCTTCCGTATGA

Upstream 100 bases:

>100_bases
TGGCCGACCGTCCGCCGGTGGCCACCCCCGCTCTCCGCTCCGAGTCCGCCCCGTCCGTCTCTTCGTCCCACGCCGCTTTC
GATCAAGGGAGTTCCCCATC

Downstream 100 bases:

>100_bases
GCCGTTTTCTCGACCACCTGCGCAATCGGGTCTTGCTGTGCGACGGCGGCATGGGAAGTTTGGTCCAGGCCATGGACCTG
TCGGTGGACGGCGACTTCAT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTVTASPLIARPRAPRALSVSFEFFPPKTDRMRDQLWDCLRQLEPLEPSFVSVTYGAGGSTRERTHETVARIAADTTMKP
AAHLTCVEATRAEVNAVAQAYWQAGVRHVVALRGDPPTLGGAYVPHPGGYAYALDLVKGLRDVADFEISVAAYPEVHPQA
RSAAEDLDNLKRKIDAGATRAISQYFFDVDSYRRFMDRVLAAGISVPVVPGILPVGNFQTVLKFSNACGTRVPKWMHELF
AGLENDPETHRMVAATLAAEQCLSLFEDGVDCFHFYTLNRADLVRAVCHMLGLRPAAIPTASRLAPDQGAGTIALGASV

Sequences:

>Translated_319_residues
MTVTASPLIARPRAPRALSVSFEFFPPKTDRMRDQLWDCLRQLEPLEPSFVSVTYGAGGSTRERTHETVARIAADTTMKP
AAHLTCVEATRAEVNAVAQAYWQAGVRHVVALRGDPPTLGGAYVPHPGGYAYALDLVKGLRDVADFEISVAAYPEVHPQA
RSAAEDLDNLKRKIDAGATRAISQYFFDVDSYRRFMDRVLAAGISVPVVPGILPVGNFQTVLKFSNACGTRVPKWMHELF
AGLENDPETHRMVAATLAAEQCLSLFEDGVDCFHFYTLNRADLVRAVCHMLGLRPAAIPTASRLAPDQGAGTIALGASV
>Mature_318_residues
TVTASPLIARPRAPRALSVSFEFFPPKTDRMRDQLWDCLRQLEPLEPSFVSVTYGAGGSTRERTHETVARIAADTTMKPA
AHLTCVEATRAEVNAVAQAYWQAGVRHVVALRGDPPTLGGAYVPHPGGYAYALDLVKGLRDVADFEISVAAYPEVHPQAR
SAAEDLDNLKRKIDAGATRAISQYFFDVDSYRRFMDRVLAAGISVPVVPGILPVGNFQTVLKFSNACGTRVPKWMHELFA
GLENDPETHRMVAATLAAEQCLSLFEDGVDCFHFYTLNRADLVRAVCHMLGLRPAAIPTASRLAPDQGAGTIALGASV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=280, Percent_Identity=32.8571428571429, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1790377, Length=279, Percent_Identity=52.6881720430108, Blast_Score=313, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI25147441, Length=284, Percent_Identity=28.169014084507, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25147436, Length=284, Percent_Identity=28.169014084507, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6321313, Length=285, Percent_Identity=30.1754385964912, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=30.2721088435374, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 34635; Mature: 34504

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTASPLIARPRAPRALSVSFEFFPPKTDRMRDQLWDCLRQLEPLEPSFVSVTYGAGGS
CCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
TRERTHETVARIAADTTMKPAAHLTCVEATRAEVNAVAQAYWQAGVRHVVALRGDPPTLG
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
GAYVPHPGGYAYALDLVKGLRDVADFEISVAAYPEVHPQARSAAEDLDNLKRKIDAGATR
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AISQYFFDVDSYRRFMDRVLAAGISVPVVPGILPVGNFQTVLKFSNACGTRVPKWMHELF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHCCCCCHHHHHHHHH
AGLENDPETHRMVAATLAAEQCLSLFEDGVDCFHFYTLNRADLVRAVCHMLGLRPAAIPT
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCC
ASRLAPDQGAGTIALGASV
HHHCCCCCCCCEEEECCCC
>Mature Secondary Structure 
TVTASPLIARPRAPRALSVSFEFFPPKTDRMRDQLWDCLRQLEPLEPSFVSVTYGAGGS
CCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
TRERTHETVARIAADTTMKPAAHLTCVEATRAEVNAVAQAYWQAGVRHVVALRGDPPTLG
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
GAYVPHPGGYAYALDLVKGLRDVADFEISVAAYPEVHPQARSAAEDLDNLKRKIDAGATR
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AISQYFFDVDSYRRFMDRVLAAGISVPVVPGILPVGNFQTVLKFSNACGTRVPKWMHELF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHCCCCCHHHHHHHHH
AGLENDPETHRMVAATLAAEQCLSLFEDGVDCFHFYTLNRADLVRAVCHMLGLRPAAIPT
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCC
ASRLAPDQGAGTIALGASV
HHHCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA