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The map label for this gene is nudF [H]

Identifier: 83592860

GI number: 83592860

Start: 1795332

End: 1795964

Strand: Reverse

Name: nudF [H]

Synonym: Rru_A1525

Alternate gene names: 83592860

Gene position: 1795964-1795332 (Counterclockwise)

Preceding gene: 83592861

Following gene: 83592858

Centisome position: 41.26

GC content: 68.09

Gene sequence:

>633_bases
ATGCGACCGACCAAGGTGAGCGTTATCGAGCGGACCGCCGCCTTTCGCGGCTATTTCCATGTCGACCGCGTGCGGTTGCG
CCACGAGCTTTATTCGGGGGCGACCGGCCCGGAAATCGTCCGCGAGGTGTTCGAGCGCGGTCATGCCGCCGCCGTGCTGC
TTTATGACGCCGATGCCGATGTGGTGGTGCTGATTGAACAATTCCGCCCCGGCCCCTGGGCGGCGGGCGAAGAGCCGTGG
ATGCTCGAGATCGTCGCCGGCATCATCGACGCCGGGGAAACCGTCGAAGCGGTGGCGCGGCGCGAATGCCAGGAGGAATG
CGGCCTGATCCCCGACGAGTTGGTGCCGATCACCCGGGTTTTCGTCTCGCCCGGCGTCATGACCGAAACCGTCGCGCTGT
ATTGCGGCCGGGTCGACAGCCGCGCCGCCGGCGGCGTTCATGGGCTGGCCGAGGAAGGCGAGGATATCCGCGTTGTTCCC
ATGGCCGCCGAGGCCTTCATCGATCTGGCCCAAAGCGGCGCCCTGACCAATGCCACCGCCGCCCTGGCCGGTTATTGGTT
CGCGCTGAACCGGGCCTCGCTGCGCGCGCGCTGGCGCACCGGCGATGTCTCCCCCGGATCCTCGGCGGCCTAA

Upstream 100 bases:

>100_bases
CCGCCGAGGAAATCCGCGCCCTGGCCGGGGAAGTCCTGGCCCACCTGTCCTGATCCCTTCCCTCTTCCCTCTTCCCCTTT
ATCTTGGAGCCTGTGTGCCG

Downstream 100 bases:

>100_bases
CCCCGCCTTACCCCCCTATCGTTCGTCGAAGGCGGCGCGCAAGGCGATCAACGCCGCATCGAAGCCGTCTTCGACCGACC
GCACGGCGCGGGCCATCATC

Product: nucleoside diphosphate pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MRPTKVSVIERTAAFRGYFHVDRVRLRHELYSGATGPEIVREVFERGHAAAVLLYDADADVVVLIEQFRPGPWAAGEEPW
MLEIVAGIIDAGETVEAVARRECQEECGLIPDELVPITRVFVSPGVMTETVALYCGRVDSRAAGGVHGLAEEGEDIRVVP
MAAEAFIDLAQSGALTNATAALAGYWFALNRASLRARWRTGDVSPGSSAA

Sequences:

>Translated_210_residues
MRPTKVSVIERTAAFRGYFHVDRVRLRHELYSGATGPEIVREVFERGHAAAVLLYDADADVVVLIEQFRPGPWAAGEEPW
MLEIVAGIIDAGETVEAVARRECQEECGLIPDELVPITRVFVSPGVMTETVALYCGRVDSRAAGGVHGLAEEGEDIRVVP
MAAEAFIDLAQSGALTNATAALAGYWFALNRASLRARWRTGDVSPGSSAA
>Mature_210_residues
MRPTKVSVIERTAAFRGYFHVDRVRLRHELYSGATGPEIVREVFERGHAAAVLLYDADADVVVLIEQFRPGPWAAGEEPW
MLEIVAGIIDAGETVEAVARRECQEECGLIPDELVPITRVFVSPGVMTETVALYCGRVDSRAAGGVHGLAEEGEDIRVVP
MAAEAFIDLAQSGALTNATAALAGYWFALNRASLRARWRTGDVSPGSSAA

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=195, Percent_Identity=43.0769230769231, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI1788810, Length=147, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 22667; Mature: 22667

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPTKVSVIERTAAFRGYFHVDRVRLRHELYSGATGPEIVREVFERGHAAAVLLYDADAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCC
VVVLIEQFRPGPWAAGEEPWMLEIVAGIIDAGETVEAVARRECQEECGLIPDELVPITRV
EEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FVSPGVMTETVALYCGRVDSRAAGGVHGLAEEGEDIRVVPMAAEAFIDLAQSGALTNATA
HHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEEEEHHHHHHHHHHCCCCCHHHH
ALAGYWFALNRASLRARWRTGDVSPGSSAA
HHHHHHHHHHHHHHEEEECCCCCCCCCCCC
>Mature Secondary Structure
MRPTKVSVIERTAAFRGYFHVDRVRLRHELYSGATGPEIVREVFERGHAAAVLLYDADAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCC
VVVLIEQFRPGPWAAGEEPWMLEIVAGIIDAGETVEAVARRECQEECGLIPDELVPITRV
EEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FVSPGVMTETVALYCGRVDSRAAGGVHGLAEEGEDIRVVPMAAEAFIDLAQSGALTNATA
HHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEEEEHHHHHHHHHHCCCCCHHHH
ALAGYWFALNRASLRARWRTGDVSPGSSAA
HHHHHHHHHHHHHHEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]