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The map label for this gene is cooF

Identifier: 83592762

GI number: 83592762

Start: 1685637

End: 1686209

Strand: Direct

Name: cooF

Synonym: Rru_A1426

Alternate gene names: 83592762

Gene position: 1685637-1686209 (Clockwise)

Preceding gene: 83592761

Following gene: 83592763

Centisome position: 38.73

GC content: 63.35

Gene sequence:

>573_bases
ATGCCGCCAATCAAAGAAAATGTAATCATCTACGCCAATCCCGATCATTGTCTGTCCTGCCATTCATGCGAGTTGGCTTG
CGCCGTCGCCCATAGTGGCGGTCACGACATGATCGAGGCGATTGCCGCCAATTTGCCGCTGCATGCGCGCAACAAGGTGG
TCAGCGTCGATGGCACGGCGATGCCCATGCAGTGCCGGCAGTGCGAGGATGCGCCCTGCACCTTCGCCTGCCCGACCGGC
GCCTGTCGTCAGGCCGATGGTCAGGTTCAGATCGTCGAGCAGCACTGCATCGGTTGCAAGCTCTGCGTGATGGTCTGCCC
CTTCGGGGCGATCACCGTGCGCTCGGAAACGGTCGTCGAGCAGGGCGCCTGCACCAACCGGGGCGTCGCCAAGAAATGCG
ATCTGTGTGTCGACTGGCGCGCCAGCACCGGCAAGACGGCTCCGGCCTGTGTCGAGGCTTGCCCGACCAAGGCCATCCGC
ATGGTCGATCTCGACGCCTATCGGATCGCCTTGCGCGAGGCCCGGGCCCGCGAGATCGCCAAGTCGCACCGCCATATGCG
CGTCCAATTCTAA

Upstream 100 bases:

>100_bases
ACAGCGTTTCGCTCCAGGGAATGATTGTTGCAGACAGTGCAGTCTAAAAAGGCAAATGCGTTGTTTTGACGTGGTAATCA
ATGCGTGGAGGGCAGTACGA

Downstream 100 bases:

>100_bases
GCACCAAGGAATCGCAGTGATGACCCATCACGACTGCGCCCACTGTTCCTCCGACGCCTGCGCGACCGAAATGTTGAATT
TGGCCGAGGCCAACAGCATC

Product: 4Fe-4S ferredoxin, iron-sulfur binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MPPIKENVIIYANPDHCLSCHSCELACAVAHSGGHDMIEAIAANLPLHARNKVVSVDGTAMPMQCRQCEDAPCTFACPTG
ACRQADGQVQIVEQHCIGCKLCVMVCPFGAITVRSETVVEQGACTNRGVAKKCDLCVDWRASTGKTAPACVEACPTKAIR
MVDLDAYRIALREARAREIAKSHRHMRVQF

Sequences:

>Translated_190_residues
MPPIKENVIIYANPDHCLSCHSCELACAVAHSGGHDMIEAIAANLPLHARNKVVSVDGTAMPMQCRQCEDAPCTFACPTG
ACRQADGQVQIVEQHCIGCKLCVMVCPFGAITVRSETVVEQGACTNRGVAKKCDLCVDWRASTGKTAPACVEACPTKAIR
MVDLDAYRIALREARAREIAKSHRHMRVQF
>Mature_189_residues
PPIKENVIIYANPDHCLSCHSCELACAVAHSGGHDMIEAIAANLPLHARNKVVSVDGTAMPMQCRQCEDAPCTFACPTGA
CRQADGQVQIVEQHCIGCKLCVMVCPFGAITVRSETVVEQGACTNRGVAKKCDLCVDWRASTGKTAPACVEACPTKAIRM
VDLDAYRIALREARAREIAKSHRHMRVQF

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH). CooF is required in stoichiometric amounts in vitro for anchoring CODH to the membrane as well as

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1788811, Length=178, Percent_Identity=34.8314606741573, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI87082179, Length=177, Percent_Identity=37.2881355932203, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI2367245, Length=159, Percent_Identity=38.9937106918239, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082180, Length=179, Percent_Identity=36.8715083798883, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1789067, Length=177, Percent_Identity=37.2881355932203, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789079, Length=192, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082114, Length=196, Percent_Identity=32.1428571428571, Blast_Score=90, Evalue=8e-20,
Organism=Escherichia coli, GI1787122, Length=169, Percent_Identity=31.9526627218935, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787872, Length=165, Percent_Identity=30.3030303030303, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1787749, Length=185, Percent_Identity=34.0540540540541, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI2367345, Length=103, Percent_Identity=38.8349514563107, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI226510944, Length=96, Percent_Identity=41.6666666666667, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COOF_RHORU (P31894)

Other databases:

- EMBL:   U65510
- PIR:   B42957
- ProteinModelPortal:   P31894
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900

Pfam domain/function: PF00037 Fer4

EC number: NA

Molecular weight: Translated: 20567; Mature: 20436

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
13.2 %Cys+Met (Translated Protein)
9.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
12.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPIKENVIIYANPDHCLSCHSCELACAVAHSGGHDMIEAIAANLPLHARNKVVSVDGTA
CCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEECCCC
MPMQCRQCEDAPCTFACPTGACRQADGQVQIVEQHCIGCKLCVMVCPFGAITVRSETVVE
CCHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEHHCCCHHEEEHHCCCCEEEECHHHHHH
QGACTNRGVAKKCDLCVDWRASTGKTAPACVEACPTKAIRMVDLDAYRIALREARAREIA
CCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
KSHRHMRVQF
HHHCCEEECC
>Mature Secondary Structure 
PPIKENVIIYANPDHCLSCHSCELACAVAHSGGHDMIEAIAANLPLHARNKVVSVDGTA
CCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEECCCC
MPMQCRQCEDAPCTFACPTGACRQADGQVQIVEQHCIGCKLCVMVCPFGAITVRSETVVE
CCHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEHHCCCHHEEEHHCCCCEEEECHHHHHH
QGACTNRGVAKKCDLCVDWRASTGKTAPACVEACPTKAIRMVDLDAYRIALREARAREIA
CCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
KSHRHMRVQF
HHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1644755