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The map label for this gene is cooH

Identifier: 83592761

GI number: 83592761

Start: 1684101

End: 1685186

Strand: Direct

Name: cooH

Synonym: Rru_A1425

Alternate gene names: 83592761

Gene position: 1684101-1685186 (Clockwise)

Preceding gene: 83592760

Following gene: 83592762

Centisome position: 38.69

GC content: 60.96

Gene sequence:

>1086_bases
ATGAGCACCTATACCATTCCCGTCGGCCCGCTGCATGTGGCCCTTGAAGAGCCGATGTACTTCCGCATCGAGGTCGATGG
CGAGAAGGTGGTCAGCGTCGATATCACCGCCGGCCATGTCCATCGCGGCATCGAATACCTGGCGACCAAGCGCAACATCT
ATCAAAATATCGTGCTGACCGAGCGCGTCTGCTCGCTCTGCTCCAACAGCCATCCGCAAACTTATTGCATGGCCCTGGAA
AGCATCACCGGCATGGTGGTGCCGCCGCGCGCCCAATATCTGCGGGTGATCGCCGACGAGACCAAGCGCGTCGCCTCGCA
TATGTTCAACGTCGCCATCCTTGCCCATATCGTCGGTTTTGATTCGCTGTTCATGCATGTCATGGAAGCCCGCGAGATCA
TGCAAGACACCAAGGAAGCGGTCTTTGGCAATCGCATGGACATCGCCGCCATGGCGATCGGCGGGGTCAAATACGATCTT
GATAAGGACGGGCGCGATTATTTCATCGGTCAGCTCGATAAGCTTGAACCAACGCTGCGCGACGAGATCATCCCGCTTTA
TCAGACCAACCCGTCGATCGTTGATCGCACCCGGGGCATTGGCGTTCTGAGCGCGGCCGATTGCGTGGACTATGGCCTGA
TGGGGCCGGTGGCGCGCGGCTCGGGCCATGCCTATGACGTGCGCAAGCAGGCGCCCTATGCGGTTTATGATCGTCTGGAC
TTCGAGATGGCCCTGGGCGAGCACGGCGATGTGTGGTCGCGCGCCATGGTGCGCTGGCAAGAGGCCCTGACCTCGATCGG
GCTGATCCGCCAATGCCTGCGCGACATGCCCGACGGTCCGACGAAGGCGGGGCCGGTGCCGCCCATTCCCGCTGGCGAGG
CGGTCGCCAAGACCGAGGCGCCGCGCGGCGAGTTGATCTATTACCTGAAGACCAACGGCACCGACCGGCCCGAACGCCTG
AAATGGCGCGTTCCCACCTATATGAACTGGGATGCGCTCAATGTGATGATGGCCGGGGCGCGGATTTCCGATATCCCGCT
GATCGTCAACAGCATCGATCCCTGCATCTCCTGTACGGAACGCTGA

Upstream 100 bases:

>100_bases
GCGGAGCGATGGAGCGGCTGATCCCGCTGTCGGTTCTGGCCAATGCCGCCAGCACCAAGATGCTGTGGGAAAGCCTGACG
GTCATGCCCGAGGAGGCGAA

Downstream 100 bases:

>100_bases
ACGCCCCAGACAGAGAAGCGTTCTCATCTTCTAGTTCTGCTGGTTTTTCTTGATAAATCCCCGCGATGGCGATGAACGCT
TCGTGGGGATTTTGCCGAGT

Product: ech hydrogenase subunit E

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSTYTIPVGPLHVALEEPMYFRIEVDGEKVVSVDITAGHVHRGIEYLATKRNIYQNIVLTERVCSLCSNSHPQTYCMALE
SITGMVVPPRAQYLRVIADETKRVASHMFNVAILAHIVGFDSLFMHVMEAREIMQDTKEAVFGNRMDIAAMAIGGVKYDL
DKDGRDYFIGQLDKLEPTLRDEIIPLYQTNPSIVDRTRGIGVLSAADCVDYGLMGPVARGSGHAYDVRKQAPYAVYDRLD
FEMALGEHGDVWSRAMVRWQEALTSIGLIRQCLRDMPDGPTKAGPVPPIPAGEAVAKTEAPRGELIYYLKTNGTDRPERL
KWRVPTYMNWDALNVMMAGARISDIPLIVNSIDPCISCTER

Sequences:

>Translated_361_residues
MSTYTIPVGPLHVALEEPMYFRIEVDGEKVVSVDITAGHVHRGIEYLATKRNIYQNIVLTERVCSLCSNSHPQTYCMALE
SITGMVVPPRAQYLRVIADETKRVASHMFNVAILAHIVGFDSLFMHVMEAREIMQDTKEAVFGNRMDIAAMAIGGVKYDL
DKDGRDYFIGQLDKLEPTLRDEIIPLYQTNPSIVDRTRGIGVLSAADCVDYGLMGPVARGSGHAYDVRKQAPYAVYDRLD
FEMALGEHGDVWSRAMVRWQEALTSIGLIRQCLRDMPDGPTKAGPVPPIPAGEAVAKTEAPRGELIYYLKTNGTDRPERL
KWRVPTYMNWDALNVMMAGARISDIPLIVNSIDPCISCTER
>Mature_360_residues
STYTIPVGPLHVALEEPMYFRIEVDGEKVVSVDITAGHVHRGIEYLATKRNIYQNIVLTERVCSLCSNSHPQTYCMALES
ITGMVVPPRAQYLRVIADETKRVASHMFNVAILAHIVGFDSLFMHVMEAREIMQDTKEAVFGNRMDIAAMAIGGVKYDLD
KDGRDYFIGQLDKLEPTLRDEIIPLYQTNPSIVDRTRGIGVLSAADCVDYGLMGPVARGSGHAYDVRKQAPYAVYDRLDF
EMALGEHGDVWSRAMVRWQEALTSIGLIRQCLRDMPDGPTKAGPVPPIPAGEAVAKTEAPRGELIYYLKTNGTDRPERLK
WRVPTYMNWDALNVMMAGARISDIPLIVNSIDPCISCTER

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH)

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase

Homologues:

Organism=Homo sapiens, GI4758786, Length=384, Percent_Identity=31.5104166666667, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI260898743, Length=374, Percent_Identity=31.8181818181818, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1789076, Length=360, Percent_Identity=37.2222222222222, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1788832, Length=357, Percent_Identity=35.2941176470588, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI145693162, Length=386, Percent_Identity=27.979274611399, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17555284, Length=394, Percent_Identity=27.6649746192893, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17568379, Length=394, Percent_Identity=27.6649746192893, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24638644, Length=384, Percent_Identity=30.7291666666667, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI221459469, Length=380, Percent_Identity=28.6842105263158, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COOH_RHORU (P31895)

Other databases:

- EMBL:   U65510
- PIR:   T51319
- ProteinModelPortal:   P31895
- SMR:   P31895
- InterPro:   IPR001135
- InterPro:   IPR001501

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases

EC number: NA

Molecular weight: Translated: 40281; Mature: 40150

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTYTIPVGPLHVALEEPMYFRIEVDGEKVVSVDITAGHVHRGIEYLATKRNIYQNIVLT
CCEEEECCCCEEEEECCCEEEEEEECCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
ERVCSLCSNSHPQTYCMALESITGMVVPPRAQYLRVIADETKRVASHMFNVAILAHIVGF
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSLFMHVMEAREIMQDTKEAVFGNRMDIAAMAIGGVKYDLDKDGRDYFIGQLDKLEPTLR
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEECCCCCCEEECCCHHHCCHHHH
DEIIPLYQTNPSIVDRTRGIGVLSAADCVDYGLMGPVARGSGHAYDVRKQAPYAVYDRLD
HCEEEEECCCCCHHHHCCCCCEEEHHHHHHHCCCCCCCCCCCCEEEHHCCCCCHHHHCCC
FEMALGEHGDVWSRAMVRWQEALTSIGLIRQCLRDMPDGPTKAGPVPPIPAGEAVAKTEA
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCC
PRGELIYYLKTNGTDRPERLKWRVPTYMNWDALNVMMAGARISDIPLIVNSIDPCISCTE
CCCCEEEEEECCCCCCCHHEEEECCCEECCHHHHHEEECCCCCCCCEEECCCCHHHHCCC
R
C
>Mature Secondary Structure 
STYTIPVGPLHVALEEPMYFRIEVDGEKVVSVDITAGHVHRGIEYLATKRNIYQNIVLT
CEEEECCCCEEEEECCCEEEEEEECCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
ERVCSLCSNSHPQTYCMALESITGMVVPPRAQYLRVIADETKRVASHMFNVAILAHIVGF
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSLFMHVMEAREIMQDTKEAVFGNRMDIAAMAIGGVKYDLDKDGRDYFIGQLDKLEPTLR
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEECCCCCCEEECCCHHHCCHHHH
DEIIPLYQTNPSIVDRTRGIGVLSAADCVDYGLMGPVARGSGHAYDVRKQAPYAVYDRLD
HCEEEEECCCCCHHHHCCCCCEEEHHHHHHHCCCCCCCCCCCCEEEHHCCCCCHHHHCCC
FEMALGEHGDVWSRAMVRWQEALTSIGLIRQCLRDMPDGPTKAGPVPPIPAGEAVAKTEA
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCC
PRGELIYYLKTNGTDRPERLKWRVPTYMNWDALNVMMAGARISDIPLIVNSIDPCISCTE
CCCCEEEEEECCCCCCCHHEEEECCCEECCHHHHHEEECCCCCCCCEEECCCCHHHHCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755