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The map label for this gene is hycF [C]

Identifier: 83592759

GI number: 83592759

Start: 1683089

End: 1683589

Strand: Direct

Name: hycF [C]

Synonym: Rru_A1423

Alternate gene names: 83592759

Gene position: 1683089-1683589 (Clockwise)

Preceding gene: 83592758

Following gene: 83592760

Centisome position: 38.67

GC content: 60.48

Gene sequence:

>501_bases
GTGGCATTTCTATCCATCTTGATGAAGAACCTGCTCAAGGGCCCGTCGACCGAGCCCTTGCCGACCGCCGATAGCCCCAC
CCCCGCCGCCTATCGCGGCAAGGTGACCTTTGACGAGACCGCCTGCGTCGGCTGCAAGATGTGCGAGCACGTCTGCCCGG
GCGGGGCGATCCGCTTCGAGGAGCGGCCCGAGGGGCTGCGCTTCATGATCTGGCACAACACCTGCGTCAATTGCGGCCTG
TGCTCCCACTATTGCCTGACCAAGGCGATAAAACTTAGCAATGACTGGCATTTGTCGCATTTGCAGGCAGACAAGTATCG
CCTGACCGATCAAGCCCTTGTCGCTTATGCCGCTTGTTCGGGCTGTGGGACGAAGATGCTTCCCACCGCCGATGCCCTGA
TGCGTCTGGCTTACAAAAAGGTGAGCCCGCGTAGCGAGCATCTGCGCCATCTGTGTCCGGACTGCCGCCGCTCAGCCAGT
GTCTCAGGAGATATCCAATGA

Upstream 100 bases:

>100_bases
CGATGTCAACGTGCCGGGCTGTCCGCCGCGCCCCCAGGCGATCATCGAGGGGATCGCCAAAGCCATCGAAATTTGGGCCG
GTCGGATCTAGGAGGGCGTC

Downstream 100 bases:

>100_bases
CCCCTCATCCCACGCCGCAAGAGCTGGTGCGCGTCACGGTCAAGGCCCTCAACGGCCAACTCGCCATCGAGGAGCCCGCC
GGCGCGCCGATGTCGGTCTG

Product: 4Fe-4S ferredoxin, iron-sulfur binding

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: Hydrogenase-4 Component H; Formate Hydrogenlyase Complex Iron-Sulfur Subunit; NAD(P)H-Quinone Oxidoreductase Subunit I; Fe-Hydrogenase Subunit Beta; NADH Dehydrogenase Subunit; Membrane-Bound Hydrogenase MBH 1 Subunit Mbh1N; Hydrogenase Subunit CooX

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MAFLSILMKNLLKGPSTEPLPTADSPTPAAYRGKVTFDETACVGCKMCEHVCPGGAIRFEERPEGLRFMIWHNTCVNCGL
CSHYCLTKAIKLSNDWHLSHLQADKYRLTDQALVAYAACSGCGTKMLPTADALMRLAYKKVSPRSEHLRHLCPDCRRSAS
VSGDIQ

Sequences:

>Translated_166_residues
MAFLSILMKNLLKGPSTEPLPTADSPTPAAYRGKVTFDETACVGCKMCEHVCPGGAIRFEERPEGLRFMIWHNTCVNCGL
CSHYCLTKAIKLSNDWHLSHLQADKYRLTDQALVAYAACSGCGTKMLPTADALMRLAYKKVSPRSEHLRHLCPDCRRSAS
VSGDIQ
>Mature_165_residues
AFLSILMKNLLKGPSTEPLPTADSPTPAAYRGKVTFDETACVGCKMCEHVCPGGAIRFEERPEGLRFMIWHNTCVNCGLC
SHYCLTKAIKLSNDWHLSHLQADKYRLTDQALVAYAACSGCGTKMLPTADALMRLAYKKVSPRSEHLRHLCPDCRRSASV
SGDIQ

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 18320; Mature: 18189

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
10.8 %Cys+Met (Translated Protein)
7.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
10.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLSILMKNLLKGPSTEPLPTADSPTPAAYRGKVTFDETACVGCKMCEHVCPGGAIRFE
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCEEECCHHHHHHHHHHHHCCCCCEEEC
ERPEGLRFMIWHNTCVNCGLCSHYCLTKAIKLSNDWHLSHLQADKYRLTDQALVAYAACS
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHC
GCGTKMLPTADALMRLAYKKVSPRSEHLRHLCPDCRRSASVSGDIQ
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHCCCCCCCCC
>Mature Secondary Structure 
AFLSILMKNLLKGPSTEPLPTADSPTPAAYRGKVTFDETACVGCKMCEHVCPGGAIRFE
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCEEECCHHHHHHHHHHHHCCCCCEEEC
ERPEGLRFMIWHNTCVNCGLCSHYCLTKAIKLSNDWHLSHLQADKYRLTDQALVAYAACS
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHC
GCGTKMLPTADALMRLAYKKVSPRSEHLRHLCPDCRRSASVSGDIQ
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA