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The map label for this gene is hycG [C]

Identifier: 83592758

GI number: 83592758

Start: 1682651

End: 1683079

Strand: Direct

Name: hycG [C]

Synonym: Rru_A1422

Alternate gene names: 83592758

Gene position: 1682651-1683079 (Clockwise)

Preceding gene: 83592757

Following gene: 83592759

Centisome position: 38.66

GC content: 61.77

Gene sequence:

>429_bases
ATGAATTTTCTTTCGCGTATGTCCAAGAAGTCGCCCTGGCTTTACCGCATCAACGCCGGATCGTGTAACGGCTGCGATGT
CGAGCTGGCGACCACCGCCTGCATTCCGCGCTATGACGTCGAACGCCTTGGCTGTCAGTACTGCGGCAGCCCCAAGCACG
CCGATATCGTGCTGGTCACCGGCCCTTTGACCGCCCGGGTCAAGGACAAGGTGCTGCGGGTCTATGAGGAAATCCCCGAT
CCCAAGGTCACCGTAGCCATCGGGGTTTGCCCGATCTCGGGCGGGGTGTTCCGGGAAAGCTACAGCATCGTCGGCCCGAT
CGACCGTTATCTGCCCGTCGATGTCAACGTGCCGGGCTGTCCGCCGCGCCCCCAGGCGATCATCGAGGGGATCGCCAAAG
CCATCGAAATTTGGGCCGGTCGGATCTAG

Upstream 100 bases:

>100_bases
GCGCATCGATCAGGCGGTGATCTTTTATCTGAAATGGCCAGAGCTTCTGGGCATCGCCAGCCTTGCCCTGGTGGCAATGA
ACCTGTGAGGGATGACGGCC

Downstream 100 bases:

>100_bases
GAGGGCGTCGTGGCATTTCTATCCATCTTGATGAAGAACCTGCTCAAGGGCCCGTCGACCGAGCCCTTGCCGACCGCCGA
TAGCCCCACCCCCGCCGCCT

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MNFLSRMSKKSPWLYRINAGSCNGCDVELATTACIPRYDVERLGCQYCGSPKHADIVLVTGPLTARVKDKVLRVYEEIPD
PKVTVAIGVCPISGGVFRESYSIVGPIDRYLPVDVNVPGCPPRPQAIIEGIAKAIEIWAGRI

Sequences:

>Translated_142_residues
MNFLSRMSKKSPWLYRINAGSCNGCDVELATTACIPRYDVERLGCQYCGSPKHADIVLVTGPLTARVKDKVLRVYEEIPD
PKVTVAIGVCPISGGVFRESYSIVGPIDRYLPVDVNVPGCPPRPQAIIEGIAKAIEIWAGRI
>Mature_142_residues
MNFLSRMSKKSPWLYRINAGSCNGCDVELATTACIPRYDVERLGCQYCGSPKHADIVLVTGPLTARVKDKVLRVYEEIPD
PKVTVAIGVCPISGGVFRESYSIVGPIDRYLPVDVNVPGCPPRPQAIIEGIAKAIEIWAGRI

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=126, Percent_Identity=36.5079365079365, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789074, Length=139, Percent_Identity=41.0071942446043, Blast_Score=124, Evalue=3e-30,
Organism=Escherichia coli, GI1788834, Length=134, Percent_Identity=41.7910447761194, Blast_Score=121, Evalue=2e-29,
Organism=Escherichia coli, GI1788624, Length=124, Percent_Identity=36.2903225806452, Blast_Score=85, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=109, Percent_Identity=36.697247706422, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI18859983, Length=123, Percent_Identity=36.5853658536585, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24642371, Length=123, Percent_Identity=36.5853658536585, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24651058, Length=109, Percent_Identity=39.4495412844037, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 15503; Mature: 15503

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLSRMSKKSPWLYRINAGSCNGCDVELATTACIPRYDVERLGCQYCGSPKHADIVLVT
CCHHHHHHCCCCEEEEEECCCCCCCCEEEEHEECCCCCCHHHHHHHHCCCCCCCEEEEEE
GPLTARVKDKVLRVYEEIPDPKVTVAIGVCPISGGVFRESYSIVGPIDRYLPVDVNVPGC
CCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEECCCCEECCCCCCEEEECCCCCC
PPRPQAIIEGIAKAIEIWAGRI
CCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNFLSRMSKKSPWLYRINAGSCNGCDVELATTACIPRYDVERLGCQYCGSPKHADIVLVT
CCHHHHHHCCCCEEEEEECCCCCCCCEEEEHEECCCCCCHHHHHHHHCCCCCCCEEEEEE
GPLTARVKDKVLRVYEEIPDPKVTVAIGVCPISGGVFRESYSIVGPIDRYLPVDVNVPGC
CCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEECCCCEECCCCCCEEEECCCCCC
PPRPQAIIEGIAKAIEIWAGRI
CCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]