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The map label for this gene is hycD [H]

Identifier: 83592757

GI number: 83592757

Start: 1681667

End: 1682638

Strand: Direct

Name: hycD [H]

Synonym: Rru_A1421

Alternate gene names: 83592757

Gene position: 1681667-1682638 (Clockwise)

Preceding gene: 83592756

Following gene: 83592758

Centisome position: 38.63

GC content: 63.27

Gene sequence:

>972_bases
ATGACTGATAGCCTTGTTCAAACCCTGTCCGTGCTGTTCGCGGCGGTGGTCTTTCCCGGCGGGATCTTCGCCATCACCCT
GGGGCTCGCCCTCAAGGGCTTTGACCGGCGGGTCTTCGCCCGGCTGCAACGCCGGGTGGGCGCCCCCTTGCTCCAGCCGT
TCTATGACGTGCTGAAGCTGATGACCAAGCGCACCCTAGTGCCCGAGAACGCCAATCTGGTGGTGTTTTTGATGGTGCCG
CTGCTGGGGCTGGCCAGCATGGGCGTGGCGGCGGCGATGATCCCGATCCCCGGGCTTTATGATCCAAGCCCGGCCTTCGG
CGACTTGTTGGTGCTGTTTTACCTGCTCTCGGTGCCGGCGGTGATGCTGATGCTGGCGGGATCGGCTTCGGGGTCGCCCT
TCGGCGGCATCGGCTTTTCGCGCGAGATGGCGATCATGCTGGCCTATGAGGGGCCGATCCTGCTGGTCATCGTCAGCGTG
GCGCTGAAGACCGGGGCGGCCCTTGGCCAGCCGATCTGCCTGTCGCTCTCGGAGATCGTGCGCTACCAGCAAGCCCACGG
CGCCTATCTGTTCGATCCGTGGATGTGGCCGGCGGTGCTGACCTATATCGCCTTCATTCCGGCCAATCTCGGCATCTCGC
CCTTTGATATTCCCGAGGCGGAATCCGAGGTGCTGGAAGGGCCGTTGATCGAATATTCCGGCGTCGCCCTTGGCCTGTTG
AAGCTGACCTCGGCGGTCAAATCCGTCGTCGTCATCGGCCTGGGCATCGTTTTGTTCTTCCCCAATGGCCCCGAGGGCCT
TTTCGGGCTGGTGGTCTTCGCCCTGAAATGCCTGGTCATCACCGTCTGCGGGGTGTCGGTGCTGCGCGCCGCCGTCGGCC
GGATGCGCATCGATCAGGCGGTGATCTTTTATCTGAAATGGCCAGAGCTTCTGGGCATCGCCAGCCTTGCCCTGGTGGCA
ATGAACCTGTGA

Upstream 100 bases:

>100_bases
GCTTCCGCCATGCATGTCCCGGCTTCGGGTCTGTACGAGACACCCGATCGCCTGATCCGCAAGGTTCTTTTCGCCAAGAC
TTCGCCCGAGGGCCGCGCCC

Downstream 100 bases:

>100_bases
GGGATGACGGCCATGAATTTTCTTTCGCGTATGTCCAAGAAGTCGCCCTGGCTTTACCGCATCAACGCCGGATCGTGTAA
CGGCTGCGATGTCGAGCTGG

Product: membrane bound hydrogenase subunit mbhM

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTDSLVQTLSVLFAAVVFPGGIFAITLGLALKGFDRRVFARLQRRVGAPLLQPFYDVLKLMTKRTLVPENANLVVFLMVP
LLGLASMGVAAAMIPIPGLYDPSPAFGDLLVLFYLLSVPAVMLMLAGSASGSPFGGIGFSREMAIMLAYEGPILLVIVSV
ALKTGAALGQPICLSLSEIVRYQQAHGAYLFDPWMWPAVLTYIAFIPANLGISPFDIPEAESEVLEGPLIEYSGVALGLL
KLTSAVKSVVVIGLGIVLFFPNGPEGLFGLVVFALKCLVITVCGVSVLRAAVGRMRIDQAVIFYLKWPELLGIASLALVA
MNL

Sequences:

>Translated_323_residues
MTDSLVQTLSVLFAAVVFPGGIFAITLGLALKGFDRRVFARLQRRVGAPLLQPFYDVLKLMTKRTLVPENANLVVFLMVP
LLGLASMGVAAAMIPIPGLYDPSPAFGDLLVLFYLLSVPAVMLMLAGSASGSPFGGIGFSREMAIMLAYEGPILLVIVSV
ALKTGAALGQPICLSLSEIVRYQQAHGAYLFDPWMWPAVLTYIAFIPANLGISPFDIPEAESEVLEGPLIEYSGVALGLL
KLTSAVKSVVVIGLGIVLFFPNGPEGLFGLVVFALKCLVITVCGVSVLRAAVGRMRIDQAVIFYLKWPELLGIASLALVA
MNL
>Mature_322_residues
TDSLVQTLSVLFAAVVFPGGIFAITLGLALKGFDRRVFARLQRRVGAPLLQPFYDVLKLMTKRTLVPENANLVVFLMVPL
LGLASMGVAAAMIPIPGLYDPSPAFGDLLVLFYLLSVPAVMLMLAGSASGSPFGGIGFSREMAIMLAYEGPILLVIVSVA
LKTGAALGQPICLSLSEIVRYQQAHGAYLFDPWMWPAVLTYIAFIPANLGISPFDIPEAESEVLEGPLIEYSGVALGLLK
LTSAVKSVVVIGLGIVLFFPNGPEGLFGLVVFALKCLVITVCGVSVLRAAVGRMRIDQAVIFYLKWPELLGIASLALVAM
NL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Homo sapiens, GI251831107, Length=289, Percent_Identity=25.2595155709343, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789077, Length=277, Percent_Identity=30.6859205776173, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87082115, Length=239, Percent_Identity=32.2175732217573, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1788618, Length=302, Percent_Identity=29.1390728476821, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 34371; Mature: 34240

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSLVQTLSVLFAAVVFPGGIFAITLGLALKGFDRRVFARLQRRVGAPLLQPFYDVLKL
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHH
MTKRTLVPENANLVVFLMVPLLGLASMGVAAAMIPIPGLYDPSPAFGDLLVLFYLLSVPA
HHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
VMLMLAGSASGSPFGGIGFSREMAIMLAYEGPILLVIVSVALKTGAALGQPICLSLSEIV
HHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCHHCCCHHHHHHHHHH
RYQQAHGAYLFDPWMWPAVLTYIAFIPANLGISPFDIPEAESEVLEGPLIEYSGVALGLL
HHHHHCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEECCCHHHHHH
KLTSAVKSVVVIGLGIVLFFPNGPEGLFGLVVFALKCLVITVCGVSVLRAAVGRMRIDQA
HHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIFYLKWPELLGIASLALVAMNL
HEEEEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDSLVQTLSVLFAAVVFPGGIFAITLGLALKGFDRRVFARLQRRVGAPLLQPFYDVLKL
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHH
MTKRTLVPENANLVVFLMVPLLGLASMGVAAAMIPIPGLYDPSPAFGDLLVLFYLLSVPA
HHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
VMLMLAGSASGSPFGGIGFSREMAIMLAYEGPILLVIVSVALKTGAALGQPICLSLSEIV
HHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCHHCCCHHHHHHHHHH
RYQQAHGAYLFDPWMWPAVLTYIAFIPANLGISPFDIPEAESEVLEGPLIEYSGVALGLL
HHHHHCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEECCCHHHHHH
KLTSAVKSVVVIGLGIVLFFPNGPEGLFGLVVFALKCLVITVCGVSVLRAAVGRMRIDQA
HHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIFYLKWPELLGIASLALVAMNL
HEEEEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]