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The map label for this gene is nifH [H]

Identifier: 83592731

GI number: 83592731

Start: 1646660

End: 1647487

Strand: Reverse

Name: nifH [H]

Synonym: Rru_A1395

Alternate gene names: 83592731

Gene position: 1647487-1646660 (Counterclockwise)

Preceding gene: 83592732

Following gene: 83592730

Centisome position: 37.85

GC content: 57.97

Gene sequence:

>828_bases
ATGACCCGCAAGATTGCTATTTATGGCAAGGGTGGAATCGGCAAGTCGACCACCACGCAGAACACCGCCGCCGCCATGGC
GCACTTCCACCACAAAAAGATTTTCATTCACGGCTGCGATCCCAAGGCGGATTCGACTAGGCTGATCCTTGGTGGCAAGC
CCCAGGAAACCCTGATGGACGTGCTGCGCGAGCAAGGGGCCGAGAAGGTCACCTACGACAAGGTCGTCCGCAAGGGCTAC
ATGGATATCCAGTGCGTTGAATCGGGCGGTCCCGAGCCCGGCGTCGGCTGCGCCGGTCGCGGCGTCATCACCGCCATCGA
CCTGATGGAAGCCCAGGGCGCCTATACCTCGGATCTCGATTTCGTCTTCTTCGACGTGCTGGGCGACGTGGTCTGTGGCG
GCTTCGCCATGCCGATCCGCGATGGCAAGGCCCAGGAGGTCTATATCGTCGCCTCGGGCGAGATGATGGCCGTCTATGCC
GCCAACAACATCTGCAAGGGTCTGGTGAAATACGCCAAGCAAAGCGGCGTGCGCCTGGGCGGCATCATCTGCAACAGCCG
CAAAGTCGATGGCGAGCGCGAGTTCATCGAGGAATTCACCAAGGCCATCGGCACGACGATGATCCATTTCGTGCCGCGCG
ACAATATCGTGCAAAAGGCCGAGTTCAACAAAAAGACCGTGACCGAATACGAGCCGACCGAAAATCAGGCCAATGAATAC
AGCCTGCTGGCTCAGGCGATCATCGAGAATGACAACTTCGTGATCCCCAAGCCGATGACCATGGATGAACTGGAAAGCAT
GGTCGAAAAGTACGGTCTTCTTGATTAA

Upstream 100 bases:

>100_bases
TTCCAGACTTTTCGGCGCCAAGCAAAGAAGACCCCAGGAAGGCAGGACTAAAAAACGCGTCCTTGGGCATTCTTTTAACC
GCCAACCAGGAGAGATCACG

Downstream 100 bases:

>100_bases
TTCCGCGGCTGGCCGAACGTTTCATTGGCGTTAAGGAGCACGCAACCAATGCCCCACCATACCTTCAAATGCAGCGAATG
CATCCCCGAGCGCACCAAGC

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein 2; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTRKIAIYGKGGIGKSTTTQNTAAAMAHFHHKKIFIHGCDPKADSTRLILGGKPQETLMDVLREQGAEKVTYDKVVRKGY
MDIQCVESGGPEPGVGCAGRGVITAIDLMEAQGAYTSDLDFVFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAVYA
ANNICKGLVKYAKQSGVRLGGIICNSRKVDGEREFIEEFTKAIGTTMIHFVPRDNIVQKAEFNKKTVTEYEPTENQANEY
SLLAQAIIENDNFVIPKPMTMDELESMVEKYGLLD

Sequences:

>Translated_275_residues
MTRKIAIYGKGGIGKSTTTQNTAAAMAHFHHKKIFIHGCDPKADSTRLILGGKPQETLMDVLREQGAEKVTYDKVVRKGY
MDIQCVESGGPEPGVGCAGRGVITAIDLMEAQGAYTSDLDFVFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAVYA
ANNICKGLVKYAKQSGVRLGGIICNSRKVDGEREFIEEFTKAIGTTMIHFVPRDNIVQKAEFNKKTVTEYEPTENQANEY
SLLAQAIIENDNFVIPKPMTMDELESMVEKYGLLD
>Mature_274_residues
TRKIAIYGKGGIGKSTTTQNTAAAMAHFHHKKIFIHGCDPKADSTRLILGGKPQETLMDVLREQGAEKVTYDKVVRKGYM
DIQCVESGGPEPGVGCAGRGVITAIDLMEAQGAYTSDLDFVFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAVYAA
NNICKGLVKYAKQSGVRLGGIICNSRKVDGEREFIEEFTKAIGTTMIHFVPRDNIVQKAEFNKKTVTEYEPTENQANEYS
LLAQAIIENDNFVIPKPMTMDELESMVEKYGLLD

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein (component 2) and a component 1 which is either a molybdenum-iron protein, a vanadium-iron, or an iron-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 30127; Mature: 29996

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKIAIYGKGGIGKSTTTQNTAAAMAHFHHKKIFIHGCDPKADSTRLILGGKPQETLMD
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCHHHHHH
VLREQGAEKVTYDKVVRKGYMDIQCVESGGPEPGVGCAGRGVITAIDLMEAQGAYTSDLD
HHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCH
FVFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAVYAANNICKGLVKYAKQSGVRLG
HHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCEEE
GIICNSRKVDGEREFIEEFTKAIGTTMIHFVPRDNIVQKAEFNKKTVTEYEPTENQANEY
EEEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCHHH
SLLAQAIIENDNFVIPKPMTMDELESMVEKYGLLD
HHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRKIAIYGKGGIGKSTTTQNTAAAMAHFHHKKIFIHGCDPKADSTRLILGGKPQETLMD
CCEEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCHHHHHH
VLREQGAEKVTYDKVVRKGYMDIQCVESGGPEPGVGCAGRGVITAIDLMEAQGAYTSDLD
HHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCH
FVFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAVYAANNICKGLVKYAKQSGVRLG
HHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCEEE
GIICNSRKVDGEREFIEEFTKAIGTTMIHFVPRDNIVQKAEFNKKTVTEYEPTENQANEY
EEEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCHHH
SLLAQAIIENDNFVIPKPMTMDELESMVEKYGLLD
HHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332060 [H]