| Definition | Rhodospirillum rubrum ATCC 11170 chromosome, complete genome. |
|---|---|
| Accession | NC_007643 |
| Length | 4,352,825 |
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The map label for this gene is anfD [H]
Identifier: 83592730
GI number: 83592730
Start: 1645052
End: 1646611
Strand: Reverse
Name: anfD [H]
Synonym: Rru_A1394
Alternate gene names: 83592730
Gene position: 1646611-1645052 (Counterclockwise)
Preceding gene: 83592731
Following gene: 83592729
Centisome position: 37.83
GC content: 59.87
Gene sequence:
>1560_bases ATGCCCCACCATACCTTCAAATGCAGCGAATGCATCCCCGAGCGCACCAAGCACGCGGTGATCAAGGGCGCCGACGAGGA CCTGACCTCGTGCCTGCCGCTGGGTTATCTCAATACCATTCCGGGATCGATCTCCGAGCGCGGCTGCGCCTATTGCGGCG CCAAGCACGTGATCGGCCAGCCGATGAAGGACGTCATCCATATCAGTCACGGACCGATCGGATGCACCTACGACACCTGG CAGACCAAGCGTTATATCAGCGATAACAACAACTTCCAGCTCAAGTACACCTATGCCACCGATGTTCGGGAAAAGCACAT CGTCTTTGGCGCCGAGAAGCTTCTCAAGCAGAACATCCTCGAAGCCTTCAAGGCGTTTCCCGACATCAAGCGCATGACCA TCTACCAGACCTGCGCCACGGCGCTGATCGGCGATGACATCGACGCCATCGCCTCGGAAGTGATGGACGAGTTGCCCGAT GTCGACATCTTCGTCTGCAACTCGCCGGGCTTCGCCGGGCCCAGCCAGTCGGGCGGCCACCACAAGATCAATATCGCCTG GGTCAACCAGAAGGTCGGCACCGTCGAGCCCGAGATCACCAGCGATTACGTCATCAACTATGTTGGTGAGTATAATATCC AGGGCGATCAGGAAGTTATGCAGGATTACTTCAATCGCATGGGCATCCAGATCCTGTCGACCTTCACCGGCAACGGATCC TATGACGGCCTGCGGGCGATGCACCGCGCCCATCTCAATGTGCTCGAATGCGCCCGTTCGGCCGAATACATCTGCAATGA ATTGCGCGTGCGCTATGGCATCCCGCGCCTTGATATCGACGGCTTCGGCTTCGAACCGCTGTCGGACTCGCTGCGCAAGA TCGGCCTGTTCTTCGGCATCGAAGACCGCGCCCAGGCGATCATCGACGAAGAAACCGCCAAGTGGAAACCCCAGCTTGAT TGGTACAAGGAACGCCTGCGCGGCAAGAAGGTCTGCCTGTGGCCGGGCGGCTCCAAGCTTTGGCATTGGGCCCATGTCAT CCAGGAGGAAATGGGCCTCAACGTCGTGTCGCTCTACACCAAATTCGGCCATCAGGGCGATATGGAAAAGGGCATCGCGC GCTGCGGCGAAGGCGCCCTGGCCATCGACGATCCCAACGAGCTTGAAGGCCTGGAAGCCCTGGAGATGCTCAAGCCCGAC ATCATCTTGACGGGCAAGCGCCCGGGCGAGGTCGCCAAGAAAGTGCGCGTTCCCTATCTCAACGCCCACGCCTATCACAA CGGCCCCTATAAGGGCTACGAGGGCTGGGTGCGCTTCGCCCGCGATATCTACAACGCCATCTATTCGCCGATCTTCCAGC TGTCGGCCCTCGATATCAGCAAGGACCCGATCCCGACCGACCAGGGCTTCCTGACGCCGCAGATGATCTCCGATCCGGCC CTGCCCGCCGAGGTGCGGTCTTCGACCGTGCTGACCCCCTATCGCGGCGCTTACGACACCATTTCCGCCCTGCGCGAGAA GACCTATCCGCGCTTCGATGCCGTTCCTGTCGCCCAATAA
Upstream 100 bases:
>100_bases GACCATGGATGAACTGGAAAGCATGGTCGAAAAGTACGGTCTTCTTGATTAATTCCGCGGCTGGCCGAACGTTTCATTGG CGTTAAGGAGCACGCAACCA
Downstream 100 bases:
>100_bases CAAGGATCCCCGCCATGGACGACCTCATGAAAGACAGGATCGACCTGCTCATCGACTACATCATGAAACACTGCCTCTGG CAGTTTCACTCCCGGTCCTG
Product: nitrogenase iron-iron protein, alpha chain
Products: NA
Alternate protein names: Dinitrogenase 3 subunit alpha; Nitrogenase component I [H]
Number of amino acids: Translated: 519; Mature: 518
Protein sequence:
>519_residues MPHHTFKCSECIPERTKHAVIKGADEDLTSCLPLGYLNTIPGSISERGCAYCGAKHVIGQPMKDVIHISHGPIGCTYDTW QTKRYISDNNNFQLKYTYATDVREKHIVFGAEKLLKQNILEAFKAFPDIKRMTIYQTCATALIGDDIDAIASEVMDELPD VDIFVCNSPGFAGPSQSGGHHKINIAWVNQKVGTVEPEITSDYVINYVGEYNIQGDQEVMQDYFNRMGIQILSTFTGNGS YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSDSLRKIGLFFGIEDRAQAIIDEETAKWKPQLD WYKERLRGKKVCLWPGGSKLWHWAHVIQEEMGLNVVSLYTKFGHQGDMEKGIARCGEGALAIDDPNELEGLEALEMLKPD IILTGKRPGEVAKKVRVPYLNAHAYHNGPYKGYEGWVRFARDIYNAIYSPIFQLSALDISKDPIPTDQGFLTPQMISDPA LPAEVRSSTVLTPYRGAYDTISALREKTYPRFDAVPVAQ
Sequences:
>Translated_519_residues MPHHTFKCSECIPERTKHAVIKGADEDLTSCLPLGYLNTIPGSISERGCAYCGAKHVIGQPMKDVIHISHGPIGCTYDTW QTKRYISDNNNFQLKYTYATDVREKHIVFGAEKLLKQNILEAFKAFPDIKRMTIYQTCATALIGDDIDAIASEVMDELPD VDIFVCNSPGFAGPSQSGGHHKINIAWVNQKVGTVEPEITSDYVINYVGEYNIQGDQEVMQDYFNRMGIQILSTFTGNGS YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSDSLRKIGLFFGIEDRAQAIIDEETAKWKPQLD WYKERLRGKKVCLWPGGSKLWHWAHVIQEEMGLNVVSLYTKFGHQGDMEKGIARCGEGALAIDDPNELEGLEALEMLKPD IILTGKRPGEVAKKVRVPYLNAHAYHNGPYKGYEGWVRFARDIYNAIYSPIFQLSALDISKDPIPTDQGFLTPQMISDPA LPAEVRSSTVLTPYRGAYDTISALREKTYPRFDAVPVAQ >Mature_518_residues PHHTFKCSECIPERTKHAVIKGADEDLTSCLPLGYLNTIPGSISERGCAYCGAKHVIGQPMKDVIHISHGPIGCTYDTWQ TKRYISDNNNFQLKYTYATDVREKHIVFGAEKLLKQNILEAFKAFPDIKRMTIYQTCATALIGDDIDAIASEVMDELPDV DIFVCNSPGFAGPSQSGGHHKINIAWVNQKVGTVEPEITSDYVINYVGEYNIQGDQEVMQDYFNRMGIQILSTFTGNGSY DGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSDSLRKIGLFFGIEDRAQAIIDEETAKWKPQLDW YKERLRGKKVCLWPGGSKLWHWAHVIQEEMGLNVVSLYTKFGHQGDMEKGIARCGEGALAIDDPNELEGLEALEMLKPDI ILTGKRPGEVAKKVRVPYLNAHAYHNGPYKGYEGWVRFARDIYNAIYSPIFQLSALDISKDPIPTDQGFLTPQMISDPAL PAEVRSSTVLTPYRGAYDTISALREKTYPRFDAVPVAQ
Specific function: This iron-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation. Other nitrogenase complexes utilize a molybdenum-iron protein or a vanadium-iron protein [H]
COG id: COG2710
COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000510 - InterPro: IPR005974 - InterPro: IPR010143 - InterPro: IPR000318 - InterPro: IPR011290 [H]
Pfam domain/function: PF00148 Oxidored_nitro [H]
EC number: =1.18.6.1 [H]
Molecular weight: Translated: 58255; Mature: 58124
Theoretical pI: Translated: 6.09; Mature: 6.09
Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.3 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 4.4 %Cys+Met (Translated Protein) 2.3 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 4.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPHHTFKCSECIPERTKHAVIKGADEDLTSCLPLGYLNTIPGSISERGCAYCGAKHVIGQ CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCCCCCCCCHHHHCCHHHHCC PMKDVIHISHGPIGCTYDTWQTKRYISDNNNFQLKYTYATDVREKHIVFGAEKLLKQNIL CHHHHEEECCCCCCCCCCCHHHHHEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHH EAFKAFPDIKRMTIYQTCATALIGDDIDAIASEVMDELPDVDIFVCNSPGFAGPSQSGGH HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC HKINIAWVNQKVGTVEPEITSDYVINYVGEYNIQGDQEVMQDYFNRMGIQILSTFTGNGS EEEEEEEECCCCCCCCCCCCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHEEECCCCCC YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSDSLRKIGLFFGI CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHCCC EDRAQAIIDEETAKWKPQLDWYKERLRGKKVCLWPGGSKLWHWAHVIQEEMGLNVVSLYT CHHHHHHHCCHHHCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHH KFGHQGDMEKGIARCGEGALAIDDPNELEGLEALEMLKPDIILTGKRPGEVAKKVRVPYL HHCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCEE NAHAYHNGPYKGYEGWVRFARDIYNAIYSPIFQLSALDISKDPIPTDQGFLTPQMISDPA ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCC LPAEVRSSTVLTPYRGAYDTISALREKTYPRFDAVPVAQ CCHHHCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCC >Mature Secondary Structure PHHTFKCSECIPERTKHAVIKGADEDLTSCLPLGYLNTIPGSISERGCAYCGAKHVIGQ CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCCCCCCCCHHHHCCHHHHCC PMKDVIHISHGPIGCTYDTWQTKRYISDNNNFQLKYTYATDVREKHIVFGAEKLLKQNIL CHHHHEEECCCCCCCCCCCHHHHHEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHH EAFKAFPDIKRMTIYQTCATALIGDDIDAIASEVMDELPDVDIFVCNSPGFAGPSQSGGH HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC HKINIAWVNQKVGTVEPEITSDYVINYVGEYNIQGDQEVMQDYFNRMGIQILSTFTGNGS EEEEEEEECCCCCCCCCCCCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHEEECCCCCC YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSDSLRKIGLFFGI CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHCCC EDRAQAIIDEETAKWKPQLDWYKERLRGKKVCLWPGGSKLWHWAHVIQEEMGLNVVSLYT CHHHHHHHCCHHHCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHH KFGHQGDMEKGIARCGEGALAIDDPNELEGLEALEMLKPDIILTGKRPGEVAKKVRVPYL HHCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCEE NAHAYHNGPYKGYEGWVRFARDIYNAIYSPIFQLSALDISKDPIPTDQGFLTPQMISDPA ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCC LPAEVRSSTVLTPYRGAYDTISALREKTYPRFDAVPVAQ CCHHHCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2644222; 8392330 [H]