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The map label for this gene is aceF [C]

Identifier: 83592664

GI number: 83592664

Start: 1566530

End: 1566739

Strand: Direct

Name: aceF [C]

Synonym: Rru_A1328

Alternate gene names: 83592664

Gene position: 1566530-1566739 (Clockwise)

Preceding gene: 83592663

Following gene: 83592665

Centisome position: 35.99

GC content: 60.0

Gene sequence:

>210_bases
ATGGATATCAAAGCGAAGATGCCGTCCGTGATCGTCTCGATCGATGTCGCCGTTGGCGCCACCGTGACCAAGGGCCAGAC
CCTGGTCGTTATCGAAGCGATGAAGATGAAGAACAACGTTCCGGCCCCCATCGACGGCACCGTGAAGTCGATCGCCGTGG
CGGCTGGCGACCGGCTGAAGCCCGGCGCCTTGATGATGGTTGTTGAGTGA

Upstream 100 bases:

>100_bases
CGCCGGTTCGGTAATGAAATCGACGGGGGCTCTTCAGTCGTCCCTGTCGGCCTAAGATTGAGTATCTCTAAGACTGTGTC
TCTCAAGGAGTGAACGCACT

Downstream 100 bases:

>100_bases
GCTTGTGACTTCGGGGCGGCATGGGCCGCCCCGTTTCAAGTTTTTGCCTCTCTAGGGGGTACGTGAAATGGTGATTTACG
GTTTGTTCCTGATGGGCCTG

Product: biotin/lipoyl attachment

Products: CoA; S-acetyldihydrolipoamide; acetyl-CoA; CO2; NADH; Proton [C]

Alternate protein names: Biotin/Lipoyl Attachment Domain-Containing Protein

Number of amino acids: Translated: 69; Mature: 69

Protein sequence:

>69_residues
MDIKAKMPSVIVSIDVAVGATVTKGQTLVVIEAMKMKNNVPAPIDGTVKSIAVAAGDRLKPGALMMVVE

Sequences:

>Translated_69_residues
MDIKAKMPSVIVSIDVAVGATVTKGQTLVVIEAMKMKNNVPAPIDGTVKSIAVAAGDRLKPGALMMVVE
>Mature_69_residues
MDIKAKMPSVIVSIDVAVGATVTKGQTLVVIEAMKMKNNVPAPIDGTVKSIAVAAGDRLKPGALMMVVE

Specific function: The Pyruvate Dehydrogenase Complex Catalyzes The Overall Conversion Of Pyruvate To Acetyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: Pyruvate Dehydrogenase (E1), Dihydrolipoamide Acetyltransferase (E2) And Lipoamide Dehydrog

COG id: COG4770

COG function: function code I; Acetyl/propionyl-CoA carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.3.1.12 [C]

Molecular weight: Translated: 7121; Mature: 7121

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
8.7 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
8.7 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKAKMPSVIVSIDVAVGATVTKGQTLVVIEAMKMKNNVPAPIDGTVKSIAVAAGDRLK
CCCCCCCCEEEEEEEEECCCEECCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCC
PGALMMVVE
CCEEEEEEC
>Mature Secondary Structure
MDIKAKMPSVIVSIDVAVGATVTKGQTLVVIEAMKMKNNVPAPIDGTVKSIAVAAGDRLK
CCCCCCCCEEEEEEEEECCCEECCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCC
PGALMMVVE
CCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: Lipoyl Cofactors. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.2 {dihydrolipoamide}} [C]

Substrates: Acetyl-CoA; dihydrolipoamide; pyruvate; Coenzyme A; NAD [C]

Specific reaction: Acetyl-CoA + dihydrolipoamide = CoA + S-acetyldihydrolipoamide pyruvate + Coenzyme A + NAD = acetyl-CoA + CO2 + NADH + Proton [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl imidazole; Iodine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA