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The map label for this gene is hutC [H]

Identifier: 83592630

GI number: 83592630

Start: 1528275

End: 1529009

Strand: Direct

Name: hutC [H]

Synonym: Rru_A1294

Alternate gene names: 83592630

Gene position: 1528275-1529009 (Clockwise)

Preceding gene: 83592628

Following gene: 83592631

Centisome position: 35.11

GC content: 67.76

Gene sequence:

>735_bases
ATGTGGAAAAGCGGGCCGGTGCCCCTGTATGAGGCGGTGAAGCGCGACCTTTTGCGCAAGATCGACGAAGGGCTCTATGC
GCCCGATGCCAGACTGCCCTCGGAGAACGAGCTGGTGGCCGCCTATGGCGTATCGCGGATGACCGTTCACCGCGCCTTGC
GCGAGTTGTCCCAGCAAGGCGTTCTGGTGCGCCTGCAGGGGGTTGGAACCTTCGTCCGCCGGCCGCGCCCGCAGGCGGCG
TTGATGGAGGTGCGCGATATCACCGAGGAGATCCGGGGGCGGGGGCATCGTCATGACGCCACCCTGGTGACCCTGGAACG
GCTGGGCGCCCCGCCCTTGGTCGCCCATGCCCTTGACCTCGCCGAGGGCGCCCCGTTGTTCCATTCGCTGATCGTTCACC
GCGAGGAGGGTGTTCCGGTGCAGGTCGAGGAGCGCTTCGTTCTGCCGGCCTTCGCCCCGGACTATCTGGAGCAGGATTTC
CTCGCCACCGTCGGCACTTCGACCTATCTGCTGTCGCGCGGACCGATCAGCGAGCTTGAGCACGTCATCCAGGCCGAATG
CCCCGACGAGGCCACCCGTGCCCTGCTCGACCTTGGCGGCGGCGAGCCCTGCCTGCGGCTGGTGCGCCGCACCTGGACCG
GCGATCGCCCGGTCACCTACAGCCTGCTCACCTATCCCGGATCGCGTTACAGCCTGGGCAGTCGCCAGCGCCTGGAAAGA
GGGCGTCCCGCCTAA

Upstream 100 bases:

>100_bases
GATACGACATTTCGGCGCAAGCGGGAAAACCGGGGGAAGGGGTGTCTCCGCCTGTAATGTCCTGTATAGACAAGAGGGCA
TGCTTGTGGGAGAGCCCAGG

Downstream 100 bases:

>100_bases
TAATCGGGCGTTTTTTCAAAGTGCTGTCGCTTGTGGCAAAGCGCATTGACAGGGGCGGAGGGCGATCCATAATCCGCGAC
GGGCCGGGGTCTCGTCCTCT

Product: histidine utilization repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MWKSGPVPLYEAVKRDLLRKIDEGLYAPDARLPSENELVAAYGVSRMTVHRALRELSQQGVLVRLQGVGTFVRRPRPQAA
LMEVRDITEEIRGRGHRHDATLVTLERLGAPPLVAHALDLAEGAPLFHSLIVHREEGVPVQVEERFVLPAFAPDYLEQDF
LATVGTSTYLLSRGPISELEHVIQAECPDEATRALLDLGGGEPCLRLVRRTWTGDRPVTYSLLTYPGSRYSLGSRQRLER
GRPA

Sequences:

>Translated_244_residues
MWKSGPVPLYEAVKRDLLRKIDEGLYAPDARLPSENELVAAYGVSRMTVHRALRELSQQGVLVRLQGVGTFVRRPRPQAA
LMEVRDITEEIRGRGHRHDATLVTLERLGAPPLVAHALDLAEGAPLFHSLIVHREEGVPVQVEERFVLPAFAPDYLEQDF
LATVGTSTYLLSRGPISELEHVIQAECPDEATRALLDLGGGEPCLRLVRRTWTGDRPVTYSLLTYPGSRYSLGSRQRLER
GRPA
>Mature_244_residues
MWKSGPVPLYEAVKRDLLRKIDEGLYAPDARLPSENELVAAYGVSRMTVHRALRELSQQGVLVRLQGVGTFVRRPRPQAA
LMEVRDITEEIRGRGHRHDATLVTLERLGAPPLVAHALDLAEGAPLFHSLIVHREEGVPVQVEERFVLPAFAPDYLEQDF
LATVGTSTYLLSRGPISELEHVIQAECPDEATRALLDLGGGEPCLRLVRRTWTGDRPVTYSLLTYPGSRYSLGSRQRLER
GRPA

Specific function: Repressor which binds to the hutP region in the histidine utilization (hut) operon. It blocks the expression of all the hut genes in the absence of inducer [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082252, Length=230, Percent_Identity=30, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1786950, Length=229, Percent_Identity=25.764192139738, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010248
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 27216; Mature: 27216

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKSGPVPLYEAVKRDLLRKIDEGLYAPDARLPSENELVAAYGVSRMTVHRALRELSQQG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCC
VLVRLQGVGTFVRRPRPQAALMEVRDITEEIRGRGHRHDATLVTLERLGAPPLVAHALDL
EEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHCCCCHHHHHHHHH
AEGAPLFHSLIVHREEGVPVQVEERFVLPAFAPDYLEQDFLATVGTSTYLLSRGPISELE
HCCCHHHHHHHHHCCCCCCEEECCEEECCCCCHHHHHHHHHHHHCCHHEEECCCCHHHHH
HVIQAECPDEATRALLDLGGGEPCLRLVRRTWTGDRPVTYSLLTYPGSRYSLGSRQRLER
HHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHC
GRPA
CCCC
>Mature Secondary Structure
MWKSGPVPLYEAVKRDLLRKIDEGLYAPDARLPSENELVAAYGVSRMTVHRALRELSQQG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCC
VLVRLQGVGTFVRRPRPQAALMEVRDITEEIRGRGHRHDATLVTLERLGAPPLVAHALDL
EEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHCCCCHHHHHHHHH
AEGAPLFHSLIVHREEGVPVQVEERFVLPAFAPDYLEQDFLATVGTSTYLLSRGPISELE
HCCCHHHHHHHHHCCCCCCEEECCEEECCCCCHHHHHHHHHHHHCCHHEEECCCCHHHHH
HVIQAECPDEATRALLDLGGGEPCLRLVRRTWTGDRPVTYSLLTYPGSRYSLGSRQRLER
HHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHC
GRPA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2203753 [H]