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The map label for this gene is phoA [H]

Identifier: 83592625

GI number: 83592625

Start: 1522107

End: 1523849

Strand: Direct

Name: phoA [H]

Synonym: Rru_A1289

Alternate gene names: 83592625

Gene position: 1522107-1523849 (Clockwise)

Preceding gene: 83592624

Following gene: 83592626

Centisome position: 34.97

GC content: 63.51

Gene sequence:

>1743_bases
ATGCGGACCCTTTGCGCGACCTTGATGCTCACCACCTGCCTGGGCACCGCCGCCCAGGCCGCCACCGTCTATCCGCTCGA
TCGGGCGACGATCCTCGCCGGCTCGCCCTTCGATCTGAAAGTCGAGTTCGACGGGGCCGTCAAAGCCGCCGACGTGACGG
TGACCATCAATGGTCAGCCCGCCGCCAAGGTGTTGGGCAAGGACGTTCAGTTCGTCGAAAAGGAAGCGGGCACCGAGGCC
TCGGCCGTACTGATCCGCGCCGCCACCCTGGCCAAGCCGGGCTCCTATACCCTGACCGCCAGCGCCGGGGCGGACACCAA
AAGCGTCACCTGGGATGTTTACGGCACGCCGGCGACGGCCAAGGCCAAGAACGTCATCTTCTTCCTGGGCGACGGCCTGT
CGGTGGCCCACCGCACCGGCGCCCGCCTGATGTCCAAGGGCATGACCGAGGGCAAGGCCAACGGCCGCCTGAACATGGAC
GACCTCGACCATATGGCCTTCATCGGCACCTCGGCGACCAATGCCGTCGCCACCGATAGCGCCAATACCATGTCGGCCTA
TATGACCGGCCACAAGACGGCGGTGAACGCCCTTGGCGTCTATGCCGACCGCACCGAGGACAGCCTGGACGATCCCCGCG
TCGAGACCATTGCCGAGGCCCTGCGCCGCACCACCACGAAGTCGATCGGCGTCGTGGCGATGTCCGAGCTTGAAGACGCC
ACCCCGGCCGCCGTTTTCGCCCATACCCGCAAGCGGGCCGATAAGGCCAAGATCACCGAGATGATCTTCGATCTGAAACC
CGACGTGCTGCTCGGCGGCGGCTCGGCCTTCTTCCTGCCCAAAAGCGTCCCCGGTTCCAAGCGCAAGGACGACAAGGACT
ATGTCGCCGCGTTCAAGGACGCGGGCTATACCGTGGCGACCTCGGCCAGCGAACTGGAAGCCGCCAAGGGCACCAATAGC
GGCAAGATCCTGGGCCTTTTCCACACCGGCAATATGGATACCTATCTCGATCGCGTGCAGTTGAAGAAGGGCACCGTCGG
CAAGTTCCCCGACCAGCCCGGTCTGGTCGAGATGACCACCGTCGCCCTGGGCCAGCTTGCCAAGAACCCGGACGGCTTCT
TCCTGATGGTCGAAGGCTCGTCGATCGACAAGATGGCCCATCCGATGGATTGGGAACGCTCGATGGTCGAGACCATCGAA
TTCGATCAGGCGATCGGCGTCGCCCGCGAGTTCGTCAAAACCCATCCCGATACGCTGATCGTCGTCACCGGCGACCATAC
CCACGGCGTGTCGATCATCGGCACCATCGACGATAACAAGCCCGGTGCCGAGATGCGCGACAAGGTCGGCACCTATGCCG
ATGCCGGTTTCCCCAATTACACCGACGAAAACAAGGACGGCTATCCCGACCGCGTCGATGTCTCGCGCCGTCTGGCGATC
TTCTCGAGCAATTTCCCCGATTACTACGAAACCTTCCAGCCCAAGCTCGATGGCCCCTTCGTTCCCGCCATTCAGAACGA
GAAGAAGGAATACGTCGCCAACGAGGCCTATAAGTCGGTTCCCGGCGCCGTGTTGCGCATCGGCAATCTGCCGCGCAGCG
AAGACACCGCCGTCCATGCCGTCGACGATGTCGTTCTCCAGGCCACCGGCCCCGGCGCCGAGGGCTTCAAGGGCTATATG
GAGGAAAGCGACGTCTATCGCGTGCTGGCCGAAACCCTGGCGCTCGCTCCGGCGAAAAAGTAA

Upstream 100 bases:

>100_bases
GGGGTATTGGTTTTACTCCCAGCGCCAAGGGAACCGGAATGATCTGTACGAATTTTTCCGCGATCCCGTCAGAGCCCCTT
TTGCTCCCGAGGAGATCTCC

Downstream 100 bases:

>100_bases
GGCGCGCCGCGTTTCGATCTTCGGGCGTCCGGCGGCCTTCGCCGCCGGGCGTCGCCCTCCTGGCCGAAAGGGGTTCGTTT
CATGACCACAGGGCCGCTGT

Product: alkaline phosphatase

Products: NA

Alternate protein names: High molecular weight phosphatase; H-AP [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MRTLCATLMLTTCLGTAAQAATVYPLDRATILAGSPFDLKVEFDGAVKAADVTVTINGQPAAKVLGKDVQFVEKEAGTEA
SAVLIRAATLAKPGSYTLTASAGADTKSVTWDVYGTPATAKAKNVIFFLGDGLSVAHRTGARLMSKGMTEGKANGRLNMD
DLDHMAFIGTSATNAVATDSANTMSAYMTGHKTAVNALGVYADRTEDSLDDPRVETIAEALRRTTTKSIGVVAMSELEDA
TPAAVFAHTRKRADKAKITEMIFDLKPDVLLGGGSAFFLPKSVPGSKRKDDKDYVAAFKDAGYTVATSASELEAAKGTNS
GKILGLFHTGNMDTYLDRVQLKKGTVGKFPDQPGLVEMTTVALGQLAKNPDGFFLMVEGSSIDKMAHPMDWERSMVETIE
FDQAIGVAREFVKTHPDTLIVVTGDHTHGVSIIGTIDDNKPGAEMRDKVGTYADAGFPNYTDENKDGYPDRVDVSRRLAI
FSSNFPDYYETFQPKLDGPFVPAIQNEKKEYVANEAYKSVPGAVLRIGNLPRSEDTAVHAVDDVVLQATGPGAEGFKGYM
EESDVYRVLAETLALAPAKK

Sequences:

>Translated_580_residues
MRTLCATLMLTTCLGTAAQAATVYPLDRATILAGSPFDLKVEFDGAVKAADVTVTINGQPAAKVLGKDVQFVEKEAGTEA
SAVLIRAATLAKPGSYTLTASAGADTKSVTWDVYGTPATAKAKNVIFFLGDGLSVAHRTGARLMSKGMTEGKANGRLNMD
DLDHMAFIGTSATNAVATDSANTMSAYMTGHKTAVNALGVYADRTEDSLDDPRVETIAEALRRTTTKSIGVVAMSELEDA
TPAAVFAHTRKRADKAKITEMIFDLKPDVLLGGGSAFFLPKSVPGSKRKDDKDYVAAFKDAGYTVATSASELEAAKGTNS
GKILGLFHTGNMDTYLDRVQLKKGTVGKFPDQPGLVEMTTVALGQLAKNPDGFFLMVEGSSIDKMAHPMDWERSMVETIE
FDQAIGVAREFVKTHPDTLIVVTGDHTHGVSIIGTIDDNKPGAEMRDKVGTYADAGFPNYTDENKDGYPDRVDVSRRLAI
FSSNFPDYYETFQPKLDGPFVPAIQNEKKEYVANEAYKSVPGAVLRIGNLPRSEDTAVHAVDDVVLQATGPGAEGFKGYM
EESDVYRVLAETLALAPAKK
>Mature_580_residues
MRTLCATLMLTTCLGTAAQAATVYPLDRATILAGSPFDLKVEFDGAVKAADVTVTINGQPAAKVLGKDVQFVEKEAGTEA
SAVLIRAATLAKPGSYTLTASAGADTKSVTWDVYGTPATAKAKNVIFFLGDGLSVAHRTGARLMSKGMTEGKANGRLNMD
DLDHMAFIGTSATNAVATDSANTMSAYMTGHKTAVNALGVYADRTEDSLDDPRVETIAEALRRTTTKSIGVVAMSELEDA
TPAAVFAHTRKRADKAKITEMIFDLKPDVLLGGGSAFFLPKSVPGSKRKDDKDYVAAFKDAGYTVATSASELEAAKGTNS
GKILGLFHTGNMDTYLDRVQLKKGTVGKFPDQPGLVEMTTVALGQLAKNPDGFFLMVEGSSIDKMAHPMDWERSMVETIE
FDQAIGVAREFVKTHPDTLIVVTGDHTHGVSIIGTIDDNKPGAEMRDKVGTYADAGFPNYTDENKDGYPDRVDVSRRLAI
FSSNFPDYYETFQPKLDGPFVPAIQNEKKEYVANEAYKSVPGAVLRIGNLPRSEDTAVHAVDDVVLQATGPGAEGFKGYM
EESDVYRVLAETLALAPAKK

Specific function: Has only a phosphomonoesterase activity [H]

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Secreted. Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=504, Percent_Identity=28.7698412698413, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI94721246, Length=510, Percent_Identity=28.0392156862745, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI116734717, Length=346, Percent_Identity=30.635838150289, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI157266292, Length=350, Percent_Identity=30.5714285714286, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI294660770, Length=338, Percent_Identity=29.585798816568, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI294660772, Length=294, Percent_Identity=29.2517006802721, Blast_Score=109, Evalue=8e-24,
Organism=Escherichia coli, GI48994877, Length=359, Percent_Identity=32.8690807799443, Blast_Score=135, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320689, Length=340, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21358067, Length=496, Percent_Identity=29.6370967741936, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI19921912, Length=496, Percent_Identity=29.4354838709677, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24657835, Length=508, Percent_Identity=27.755905511811, Blast_Score=137, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24657842, Length=510, Percent_Identity=27.4509803921569, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21355151, Length=487, Percent_Identity=27.7207392197125, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI18859923, Length=463, Percent_Identity=27.4298056155508, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355981, Length=489, Percent_Identity=27.8118609406953, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24657827, Length=492, Percent_Identity=27.4390243902439, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI21355149, Length=401, Percent_Identity=28.6783042394015, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI85725200, Length=354, Percent_Identity=29.3785310734463, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI85815833, Length=354, Percent_Identity=29.3785310734463, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585213, Length=358, Percent_Identity=25.9776536312849, Blast_Score=95, Evalue=2e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 61951; Mature: 61951

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLCATLMLTTCLGTAAQAATVYPLDRATILAGSPFDLKVEFDGAVKAADVTVTINGQP
CHHHHHHHHHHHHHCCHHCCEEEEECCCEEEEECCCEEEEEEECCCEEEEEEEEEECCCC
AAKVLGKDVQFVEKEAGTEASAVLIRAATLAKPGSYTLTASAGADTKSVTWDVYGTPATA
HHHHHCCHHHHHHHHCCCCHHHEEEEHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCC
KAKNVIFFLGDGLSVAHRTGARLMSKGMTEGKANGRLNMDDLDHMAFIGTSATNAVATDS
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEECCC
ANTMSAYMTGHKTAVNALGVYADRTEDSLDDPRVETIAEALRRTTTKSIGVVAMSELEDA
CCCEEEHHCCCHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHCHHCCEEEEHHCCCC
TPAAVFAHTRKRADKAKITEMIFDLKPDVLLGGGSAFFLPKSVPGSKRKDDKDYVAAFKD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCCCCCCCCCCHHHHHHHHC
AGYTVATSASELEAAKGTNSGKILGLFHTGNMDTYLDRVQLKKGTVGKFPDQPGLVEMTT
CCEEEECCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHH
VALGQLAKNPDGFFLMVEGSSIDKMAHPMDWERSMVETIEFDQAIGVAREFVKTHPDTLI
HHHHHHHCCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VVTGDHTHGVSIIGTIDDNKPGAEMRDKVGTYADAGFPNYTDENKDGYPDRVDVSRRLAI
EEECCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEE
FSSNFPDYYETFQPKLDGPFVPAIQNEKKEYVANEAYKSVPGAVLRIGNLPRSEDTAVHA
EECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEE
VDDVVLQATGPGAEGFKGYMEESDVYRVLAETLALAPAKK
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRTLCATLMLTTCLGTAAQAATVYPLDRATILAGSPFDLKVEFDGAVKAADVTVTINGQP
CHHHHHHHHHHHHHCCHHCCEEEEECCCEEEEECCCEEEEEEECCCEEEEEEEEEECCCC
AAKVLGKDVQFVEKEAGTEASAVLIRAATLAKPGSYTLTASAGADTKSVTWDVYGTPATA
HHHHHCCHHHHHHHHCCCCHHHEEEEHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCC
KAKNVIFFLGDGLSVAHRTGARLMSKGMTEGKANGRLNMDDLDHMAFIGTSATNAVATDS
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEECCC
ANTMSAYMTGHKTAVNALGVYADRTEDSLDDPRVETIAEALRRTTTKSIGVVAMSELEDA
CCCEEEHHCCCHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHCHHCCEEEEHHCCCC
TPAAVFAHTRKRADKAKITEMIFDLKPDVLLGGGSAFFLPKSVPGSKRKDDKDYVAAFKD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCCCCCCCCCCHHHHHHHHC
AGYTVATSASELEAAKGTNSGKILGLFHTGNMDTYLDRVQLKKGTVGKFPDQPGLVEMTT
CCEEEECCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHH
VALGQLAKNPDGFFLMVEGSSIDKMAHPMDWERSMVETIEFDQAIGVAREFVKTHPDTLI
HHHHHHHCCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VVTGDHTHGVSIIGTIDDNKPGAEMRDKVGTYADAGFPNYTDENKDGYPDRVDVSRRLAI
EEECCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEE
FSSNFPDYYETFQPKLDGPFVPAIQNEKKEYVANEAYKSVPGAVLRIGNLPRSEDTAVHA
EECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEE
VDDVVLQATGPGAEGFKGYMEESDVYRVLAETLALAPAKK
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 8454193 [H]