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The map label for this gene is mcbR [H]

Identifier: 83592591

GI number: 83592591

Start: 1476687

End: 1477421

Strand: Reverse

Name: mcbR [H]

Synonym: Rru_A1255

Alternate gene names: 83592591

Gene position: 1477421-1476687 (Counterclockwise)

Preceding gene: 83592594

Following gene: 83592590

Centisome position: 33.94

GC content: 68.71

Gene sequence:

>735_bases
ATGAAACCTCCCCCGCCGCTCGACCTGCCGCAGATCGACCGCCAGCCGCGCGAGACCGTCAATCAATGGGTTTATCGTCG
GCTGCGGCTGGCGGTGATGATCGGGCGAATTCCGCCGGGGCGGCCCTTGACCATCCGCGACCTCGCCCTCGCCCTTGAGG
TCAGCACCATGCCGGTGCGCGAGGCCCTGCGCCGGCTGGCCTCGGAACGGGCGCTCGAGGTTCGCGACAACCGGCGGATC
GAGGTGCCGGTGATGACCGCCCTGAAATTCCGCGAGCTCTGCGAGGCGCGCATCGCCCTGGAATGCCACGCCGCCGAGCG
CGCCCTGCCCTTCATCACCGCCGATCGCCTAGCCTTGCTCGCCGGCCTTGACGCGCAGATCGACGCCGCCCAGGCCGCGG
GCGATCGCGATGCGCTCACCTTGCACAATCAGGCCTTCCACCGGGCGCTTTACGAGACCAACCCCCATCAGGTGGTGCTG
CCCTTGATCGAAAGCGTGTGGCTGCAGCTGGGGCCCTTCACCCGGCTCGCCCTCGCGCGCCTGGACGAGTTCTATCATGT
CGACCGCCATTCCGAGGCCCTGGCCGCCCTGCGCTCGGGCAACCCCTTGGCCCTGCGCGTCGCCATCGAGGCCGATATCC
GCGACGGCATCTTGCAAGTCGGAACCACCGAATTGCTCAACGCCTATATCGCCGCCGGCGCCACCCTGGGGACGGAGACG
GCGTTTGGCCGTTGA

Upstream 100 bases:

>100_bases
GCGCCACCGGGCGTCTCTGGACCATGGGCGGCGGATGGATTAGATGTGTTCGTAAATGTGATCATCTTTTCCCCCGTCCG
CTGGAGAGACAGCCGTCCCC

Downstream 100 bases:

>100_bases
CAGACCAAAAATGTGATCATATTTTTGTGATCACAATTGAGGCATGCCAGATCCTTGCCTAAACCCTGACCTGCCTGTCC
CGTCCTTTCTTTTTGCGGAG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKPPPPLDLPQIDRQPRETVNQWVYRRLRLAVMIGRIPPGRPLTIRDLALALEVSTMPVREALRRLASERALEVRDNRRI
EVPVMTALKFRELCEARIALECHAAERALPFITADRLALLAGLDAQIDAAQAAGDRDALTLHNQAFHRALYETNPHQVVL
PLIESVWLQLGPFTRLALARLDEFYHVDRHSEALAALRSGNPLALRVAIEADIRDGILQVGTTELLNAYIAAGATLGTET
AFGR

Sequences:

>Translated_244_residues
MKPPPPLDLPQIDRQPRETVNQWVYRRLRLAVMIGRIPPGRPLTIRDLALALEVSTMPVREALRRLASERALEVRDNRRI
EVPVMTALKFRELCEARIALECHAAERALPFITADRLALLAGLDAQIDAAQAAGDRDALTLHNQAFHRALYETNPHQVVL
PLIESVWLQLGPFTRLALARLDEFYHVDRHSEALAALRSGNPLALRVAIEADIRDGILQVGTTELLNAYIAAGATLGTET
AFGR
>Mature_244_residues
MKPPPPLDLPQIDRQPRETVNQWVYRRLRLAVMIGRIPPGRPLTIRDLALALEVSTMPVREALRRLASERALEVRDNRRI
EVPVMTALKFRELCEARIALECHAAERALPFITADRLALLAGLDAQIDAAQAAGDRDALTLHNQAFHRALYETNPHQVVL
PLIESVWLQLGPFTRLALARLDEFYHVDRHSEALAALRSGNPLALRVAIEADIRDGILQVGTTELLNAYIAAGATLGTET
AFGR

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=214, Percent_Identity=28.0373831775701, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27175; Mature: 27175

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPPPPLDLPQIDRQPRETVNQWVYRRLRLAVMIGRIPPGRPLTIRDLALALEVSTMPVR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCHHHH
EALRRLASERALEVRDNRRIEVPVMTALKFRELCEARIALECHAAERALPFITADRLALL
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AGLDAQIDAAQAAGDRDALTLHNQAFHRALYETNPHQVVLPLIESVWLQLGPFTRLALAR
HCCCCCCHHHHCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCHHHHHHHH
LDEFYHVDRHSEALAALRSGNPLALRVAIEADIRDGILQVGTTELLNAYIAAGATLGTET
HHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHCHHHHHHHHHHCCCCCCCCC
AFGR
CCCC
>Mature Secondary Structure
MKPPPPLDLPQIDRQPRETVNQWVYRRLRLAVMIGRIPPGRPLTIRDLALALEVSTMPVR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCHHHH
EALRRLASERALEVRDNRRIEVPVMTALKFRELCEARIALECHAAERALPFITADRLALL
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AGLDAQIDAAQAAGDRDALTLHNQAFHRALYETNPHQVVLPLIESVWLQLGPFTRLALAR
HCCCCCCHHHHCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCHHHHHHHH
LDEFYHVDRHSEALAALRSGNPLALRVAIEADIRDGILQVGTTELLNAYIAAGATLGTET
HHHHHHCCCHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHCHHHHHHHHHHCCCCCCCCC
AFGR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]